苏云金芽胞杆菌含不同质粒和不同基因工程菌的生长代谢

用微量热法研究了世界上产量最大的微生物杀中心剂苏云芽胞杆菌(Bacillusthuringiensis)野生菌株YBT－1463,无晶体突变株BMB160和无质粒、无晶体突变株BMB171以及含量不同杀虫蛋白基因工程菌BMB－304－171－A和BMB－304－171－B的生长代谢动力学变化。结果是,含有14个质粒的野生菌株YBT－1463的生长代谢热比无晶体含6个质粒的变株BMB160和无晶体、无质粒突变株BMB171低;将杀虫晶体蛋白基因vryⅠAc和cryⅠC分别转入受体菌BMB171中后,两个工程菌BMB－303－171－A和BMB－304－171－B生长代谢热与受体菌BMB171相比也吸显降低;表明质粒形成一个耗能过程,当受体菌BMB171转入杀虫晶体蛋白基因后,工菌比受茶杯菌的放热大幅度减少,表明基因编码杀虫晶体蛋白也是一个耗能过程,但是含基因cryⅠAc和cryⅠC工程菌BMB－304－171－A和BMB－304－171－B之间的生长代谢没有明显差异。首次报道这些热动力学变化,对杀虫剂发酵生产过程的代谢控有重要指导意义。     

芽胞杆菌全细胞热裂解气相色谱聚类分析

通过热裂解气相色谱对１４ 个芽胞杆菌模式菌株全细胞进行分析, 确定芽胞杆菌不同属间特征性谱峰;利用ＳＴＡＴＩＳＴＩＣＡ 系统对热裂解气相色谱图中３０ 个主要谱峰进行聚类分析, 绘制出的树状谱清楚地表明各菌株间的亲缘关系。     

芽胞杆菌模式菌株细胞脂肪酸组分的气相色谱分析

通过气相色谱对１０个芽胞杆菌模式菌株的细胞脂肪酸组分及其含量进行分析,经ＵＰＧＭＡ,ＮＴＳＹＳ－Ｐｃ系统聚类分析绘制的树状图谱可以清楚地将４个属的菌株分成４个不同的类群,而且同一类群中不同的种也表现出一定的差异。由此可见,控制芽胞杆菌的培养条件及生长状态,同样有效地使脂肪酸组分成为芽胞杆菌化学分类的重要指证。     

盐生盐杆菌生长过程热动力学研究

用 LKB2 2 77生物活性检测系统测定了盐生盐杆菌 R1、J7、S9以及 R1和 J7的融合子 F9生长的产热功率曲线 .根据曲线的特征 ,建立了古生菌生长过程的热动力学方程 :ln[P· ( 1 -P/Pm) r- 1 ]=ln[P0 · ( 1 -P0 /Pm) r- 1 ]+k· t.由此求得了盐生盐杆菌的生长速率常数 ,并对此模型和融合子 F9的生长进行了讨论 .该热动力学方程描述了一系列非理想的细菌生长过程的产热功率曲线 ,并将其与经典的指数式生长模型和 lo-gistic模型进行了比较 ,它具有更广泛的适用性 .首次报道了微量热技术在古生菌中的应用     

Schiff碱药物对产气杆菌代谢抑制的热力学研究

用微量热法测定产气杆菌在不同温度条件下受到Schiff碱药物抑制的热谱曲线, 根据其指数生长期的热谱信息, 确定它在不同温度条件下的生长速率常数和反应活化能, 找出了该细菌的最佳生长温度。同时借用化学反应中的过渡态理论, 得到一系列热力学参数, 对细菌在受到抑制时的生长代谢进行了热力学分析。     

营养缺陷型酿酒酵母AY生长代谢的热动力学研究

用微量热法研究了尿嘧啶缺陷型酿酒酵母AY,和该菌株分别经穿梭质粒pYLZ2、重组质粒pYLZ2/f27、pYLZ2/622转化的3种菌株的生长代谢热动力学.尿嘧啶缺陷型酿酒酵母AY在基本培养基中不生长,没有代谢热效应产生,而在葡萄糖蛋白胨培养基和丰富营养培养基(YPD)中生长,并且在YPD中生长最好;向基本培养基中加入尿嘧啶后,AY能够生长,而且随着尿嘧啶浓度的增加,其生长速率常数增大;经质粒转化的3个菌株均能在基本培养基中生长,代谢产热曲线各不相同,与转入质粒的结构、功能密切相关.研究结果表明了各菌株遗传特征的差异.     

厌氧菌生长代谢的微量量热学研究

本世纪七十年代厌氧细菌学的崛起，是近代微生物学的重要进展山．它揭示了一个前所未知或知之不多的微生物世界．在人的皮肤和粘膜表面寄生着几百种、数以亿万计的细菌，它们中绝大多数在有氧的条件下不能生长繁殖，故称为厌氧菌．厌氧细菌学的出现，引起了医务工作者的高度重视．十多年来，积累了大量资料，为医学中的微生物学、免疫学、传染病学、流行病学甚至许多临床学科，增添了新的知识，对厌氧菌的感染，用目前医院的常规细菌培养方法不能检出，用常用的抗生素治疗，也常无效．它是临床上许多疑难杂症迁延不愈和反复发作的重要原因之…     

营养缺陷型酿酒酵母AY生长代谢的热动力学研究

用微量热法研究了尿嘧啶缺陷型酿酒酵母AY,和该菌株分别经穿梭质粒pYLZ-2、重组质粒pYLZ-2/f27、pYLZ-2/622转化的3种菌株的生长代谢热动力学.尿嘧啶缺陷型酿酒酵母AY在基本培养基中不生长,没有代谢热效应产生,而在葡萄糖蛋白胨培养基和丰富营养培养基(YPD)中生长,并且在YPD中生长最好;向基本培养基中加入尿嘧啶后,AY能够生长,而且随着尿嘧啶浓度的增加,其生长速率常数增大;经质粒转化的3个菌株均能在基本培养基中生长,代谢产热曲线各不相同,与转入质粒的结构、功能密切相关.研究结果表明了各菌株遗传特征的差异.     

微量热法研究鹿茸4个活性部位对肠道特征菌群生长代谢的影响

建立灵敏表征鹿茸中具有补益活性的4个部位对两种肠道特征菌群生长代谢影响的评价方法.采用微量热法,在不同给药条件作用下,以产热功率-时间曲线(热谱曲线)的特征参数(生长速率常数(k)、最大产热功率(Pmax)、达峰时间(tp)及有效率(E)等)为指标,对肠道主要有益菌(青春双歧杆菌)生长代谢程度进行客观地量化评价.挑选鹿茸促菌最强的活性部位,进一步考察该部位对病原微生物(金黄色葡萄球菌)生长抑制的作用规律.结果表明,鹿茸不同活性部位促进青春双歧杆菌生长代谢强弱顺序为:正丁醇乙醚氯仿乙酸乙酯.活性最强的正丁醇萃取液对病原微生物(金黄色葡萄球菌)抑制影响的研究表明,正丁醇萃取液在浓度大于200μg·mL-1时,对金葡菌生长具有抑制作用.且tp随药液浓度的增加而相应地延长;k,Pmax均随浓度的增加而相应地减小.微量热法具有灵敏准确、实时在线、重现性好等特点,可用于名贵药材——鹿茸的活性部位的筛选,也为药效物质的研究提供了一个新的研究思路和方法.     

微量热法研究硒对大肠杆菌生长代谢的作用

硒 是生物必需的微量元素之一．自从１９７３年Ｒｏｔｒｕｃｋ报导了第一个硒酶———红细胞谷胱甘肽过氧化物酶［１］,硒在生命科学中的研究取得了令人瞩目的进展．继红细胞谷胱甘肽过氧化物酶之后 ,从哺乳动物中还发现硒为多种酶所必需 ;此外 ,在不同的动物组织中一系列含硒蛋白被分离和鉴别．随着分子生物学技术的发展和硒在生物化学中的应用 ,人们对硒的生物化学本质有了更加深刻的认识 ,其中 ,遗传基因硒代半胱氨酸密码子ＵＧＡ的发现使硒的生物化学研究进入了一个新的阶段．与此同时 ,小分子有机硒化合物Ｅｂｓｅｌｅｎ生物活性的发现…     

The effect of adding virgin material or extra stabilizer on the recyclability of polypropylene as studied by multi-cell imaging chemiluminescence and microcalorimetry

The effect of adding virgin material or new stabilizers on the recyclability of polypropylene (PP) was determined. Stabilized PP was subjected to oven ageing at 130 °C for 100, 250 or 500 h before and after upgrading with virgin material (0, 20, 50, 80 or 100%) or additional stabilizer during reprocessing. The effect of upgrading recycled PP with extra stabilizer or virgin material was determined by measuring the elongation at break, yellowness change, oxidation induction time (OIT) or total luminous intensity (TLI) by chemiluminescence (CL) techniques. Selected specimens were analysed by microcalorimetry (MC).It has been shown that upgrading recycled PP with virgin material was not effective. Adding 80% virgin material did not increase the lifetime more than adding 20% virgin material. This indicates that a small amount of recycled material can induce degradation by contamination. Adding extra stabilizers instead of virgin material was much more effective. Of all techniques used the OIT as determined with CL showed a clear difference in residual stability of differently treated materials and seems to be the most valuable analytical method to determine the recyclability of PP. A comparison between CL and MC shows that MC is sensitive enough for measuring the solid-state oxidation of stabilized PP. Slightly lower OITs were detected by MC probably associated with the static atmosphere used during the measurements. The TLI method is not suitable to determine the degradation state of to be recycled material.     

Structural property and function of D-erythro asymmetric chain sphingomyelins as studied by microcalorimetry and electron microscopy

D-erythro sphingomyelines (SM) having a defined acyl chain were synthesized with sphingosylphosphorylcholine as a starting material, and both a structural property and its relating phase transition phenomenon were compared between a symmetric chain length SM (palmitoyl-SM: C16-SM) and asymmetric chain length SMs (behenoyl-SM: C22-SM, lignoceryl-SM: C24-SM). Furthermore, effect of increasing a content of asymmetric chain SMs in the mixture systems of C22-SM/C16-SM, and C24-SM/C16-SM was investigated. The present calorimetric and electron microscopic studies revealed that (1) The main transition enthalpy is smaller for the asymmetric chain SMs than for the symmetric chain SM by about 3 kJ mol⁻¹, although the acyl chain length is longer for the former than for latter; (2) Relatively small size vesicles (100∼200 nm diameters) surrounded by one or more lamellae are observed for the asymmetric chain SMs, in contrast to large multilamellar vesicles (1500∼2500 nm diameters) having at least fifteen stained lamellae for the symmetric chain SM and (3) The coexisting asymmetric chain SMs cause the decrease in size and multiplicity for the MLV of the symmetric chain SM, simultaneously with a decrease in the main transition enthalpy.     

Glass transition of semi-crystalline PLLA with different morphologies as studied by dynamic mechanical analysis

Poly(l-lactic acid) was crystallized from the glassy state at different temperatures to produce fully transformed semi-crystalline specimens exhibiting different lamellar morphologies. The materials were tested by dynamic mechanical analysis, where a T _g decrease was found with an increasing crystallization temperature. Considering a three-phase model, this tendency was related to the corresponding increase in the thickness of the rigid amorphous phase. It is suggested that this phase could, in some extent, accommodate through local translational/rotational motions the cooperative motions taking place within the mobile amorphous phase. This could be due to the non-compact structure of the cooperatively rearranging regions, which can present a string-like or fractal structure in their edges. The width of the loss factor peak associated to the glass transition increases with increasing crystallization temperature, suggesting an increase in the broadness of the distribution of relaxation times. The drop in the storage modulus across T _g varies systematically with the crystallization temperature in the different materials and could be correlated with the crystalline content. Above T _g, the loss factor exhibits a plateau-like behaviour at significantly high values, which seems to be a rather general behaviour in semi-crystalline systems that could be related to the contribution of pure irreversible flow in the overall viscoelastic behaviour.     

Viscoelastic properties of chitosan with different hydration degrees as studied by dynamic mechanical analysis

Dynamic mechanical analysis, DMA, is an adequate technique for characterizing the mechanical features of biomaterials, as one can use test conditions that can more closely simulate the physiological environments in which they are going to be applied. In this work it was possible to perform different tests on chitosan membranes using low/moderate hydration levels, as well in completely wet conditions. In the first case the data obtained at different relative humidity environments were rationalized under a time-humidity superposition principle, where a master curve for the storage modulus could be obtained along a wide range of frequencies. The temperature dependence of the shift factors exhibited a curvature opposite to that expected by the WLF equation, and is consistent with relaxation dynamics behavior below the glass transition. Temperature scans above room temperature in both dry and wet conditions did not reveal strong variations in the viscoelastic properties. It was possible to follow in real time the water uptake in an initially-dry membrane. During the initial strong and fast decrease of the storage modulus the loss factor exhibited a peak that should correspond to the occurrence of the glass transition resulting from the plasticization effect of water. Upon equilibration the loss factor reached similar values as for the dry material (tandelta approximately equal to 0.5). The viscoelastic characterization reported in this work for chitosan may be useful in the use of such material for a variety of biomedical applications.     

Free‐volume change in volume phase transition of polyacrylamide gel as studied by positron annihilation: Salt dependence

Variation of free‐volume parameters—average radius size, number concentration, and size distribution—of a polyacrylamide (PAAm) gel containing 4 mol % carboxylate anions is studied during a volume phase transition (VPT) caused by a change of sodium chloride (NaCl) concentration. A positron annihilation lifetime technique is used for the determination of the free‐volume characteristics. The measurement is performed in an acetone–water 3 : 2 (v/v) [0.27 : 0.73 (mol/mol)] mixed solvent at 20°C, and the free‐volume parameters deduced from the analysis of a positron annihilation curve are utilized. An average free‐volume size of the swollen PAAm gel, ∼ 0.32 nm in radius, almost agrees with that of the mixed solvent for a corresponding salt concentration, while the size of the collapsed gel, which is ∼ 0.28 nm in radius, is smaller than that of the mixed solvent. The results for the collapsed gel indicate that the hydrogen bond plays a significant role in the nanoscopic environment. The radius of the free‐volume of the swollen PAAm gel seems to be influenced by the composition between acetone and water. An inhomogeneity of the nanoscopic structure inside the PAAm gels is discussed in terms of a dispersion of a size distribution of the free‐volume. It is concluded that a change of the nanoscopic environment of the PAAm gel during the VPT can be monitored through the free‐volume parameters obtained by the positron annihilation lifetime technique. © 1999 John Wiley & Sons, Inc. J Polym Sci B: Polym Phys 37: 2634–2641, 1999     

Complexes of Schiff base of gossypol with n-butylamine and some monovalent or bivalent cations studied by ESI MS, NMR, FT-IR as well as PM5 semiempirical methods

A Schiff base of gossypol with n-butylamine [GSBN] was shown to be capable of complexation of 2H⁺, Li⁺, Ca²⁺ and Ba²⁺ cations. This process of complex formation was studied by ESI mass spectrometry, ¹H and ¹³C NMR and FT-IR spectroscopy as well as by PM5 semiempirical method. It was found that gossypol Schiff base can form a 1:2 complex with H⁺ and 1:1 complexes with Li⁺, Ca²⁺ and Ba²⁺ cations. In all complexes the Schiff base of gossypol with metal cations exists in enamine-enamine tautomer, whereas in the 1:2 complex with H⁺ the imine-imine tautomer was found. The metal cations are coordinated through oxygen atoms of the O₁H(O₁,H) hydroxyl groups and a lone pair of an N-atom. The structures of these complexes were calculated by PM5 semiempirical method and discussed.