硫化镉纳米微粒作探针共振瑞利散射测定某些蒽环类抗癌药物

在pH=5.0—9.0的水溶液中, 硫化镉纳米微粒[(CdS)n]与蒽环类抗生素米托蒽醌(MXT)、 表柔比星(EPI)和柔红霉素(DNR)凭借静电引力及疏水作用力结合, 形成粒径更大的聚集体, 导致共振瑞利散射(RRS)的增强并产生新的RRS光谱, 最大的RRS峰位于292 nm(MXT体系)、 285 nm(DNR体系)和315 nm(EPI体系). 与此同时还观察到二级散射(SOS)和倍频散射(FDS)强度明显提高. 其最大SOS峰位于540 nm(MXT体系)和560 nm(EPI及DNR体系), 而最大的FDS峰分别位于335 nm(MXT体系)、 320 nm(EPI体系)和330 nm(DNR体系). 在一定条件下, 3种散射强度(ΔI)均与药物的浓度成正比, 反应具有高灵敏度, 对于3种药物的检出限在3.6—9.1 ng/mL之间. 其中(CdS)n-MXT体系灵敏度最高, 对MXT的检出限分别为4.1 ng/mL(RRS)、 3.8 ng/mL(SOS)和3.6 ng/mL(FDS). 据此发展了一种用纳米硫化镉作探针, 灵敏、 简便并快速测定蒽环类抗癌药物的共振瑞利散射新方法.     

金纳米微粒作探针共振瑞利散射法测定某些蒽环类抗癌药物

在近中性至弱碱性介质中, 金纳米微粒与表柔比星(EPI)、柔红霉素(DNR)和米托蒽醌(MXT)等蒽环类抗癌药物借静电引力、疏水作用力结合, 形成粒径更大的聚集体, 导致共振瑞利散射(RRS)的显著增强并产生新的RRS光谱, 三种结合产物的最大RRS峰均位于313 nm附近, 并在510～610 nm之间有一宽的散射带. 其散射强度(ΔI)与3种抗癌药物的浓度成正比, 对EPI, DNR和MXT的线性范围分别为0.009～0.50, 0.010～0.70 和0.030～1.20 μg•mL^－1, 它们的检出限(3σ)分别为2.7, 3.1和9.0 ng•mL^－1. 研究了反应产物的吸收、荧光和RRS光谱特征, 适宜的反应条件及分析化学性质, 发展了一种用RRS技术灵敏、简便、快速测定蒽环类抗癌药物的新方法.     

Resonance Rayleigh scattering spectra of ion-association nanoparticles of [Co(4-[(5-Chloro-2-pyridyl) azo]-1, 3-diaminobenzene)2]-sodium dodecyl benzene sulfonate system and its analytical application

In pH 1.8-3.0 Britton-Robinson (BR) buffer solution, cobalt (II) reacts with 4-[(5-Chloro-2-pyridyl) azo]-1, 3-diaminobenzene (5-Cl-PADAB, L) to form a cationic chelate [CoL₂]²⁺. When interacting with anionic surfactants (AS) such as sodium dodecyl benzene sulfonate (SDBS), sodium dodecyl sulfate (SDS) or sodium dodecyl sulfonate (SLS), the chelate can only react with SDBS to form ternary ion-association complexes ([CoL₂][SDBS]₂). By virtue of the extrusion action of water and Van der Waals force, the hydrophobic ion-association complexes draw close to each other and further aggregate to form {[CoL₂][SDBS]₂}_n nanoparticles with an average diameter of 30 nm. As a result, resonance Rayleigh scattering (RRS) is enhanced greatly and new RRS spectra appear. Under the same conditions, both SDS and SLS exhibit no similar reactions and do not result in obvious change of RRS. Therefore, SDBS can be determined selectively by RRS method in the presence of SDS or SLS. The wavelength of 516 nm was chosen as a detection wavelength, the linear range and the detection limit (3σ) are 0.05-6.0 μg mL⁻¹ and 0.015 μg mL⁻¹ for the determination of SDBS, respectively. The characteristics of RRS spectra of the [CoL₂]²⁺-SDBS system, the optimum conditions of the reaction and the influencing factors have been investigated. The effects of coexisting substances have been examined too, indicating a good selectivity of the method for the determination of SDBS. The method can be used for the determination of SDBS in waste water and river water samples, and the results are satisfactory compared with those of standard samples of SDBS. Based on the formation of {[CoL₂][SDBS]₂}_n nanoparticles, a sensitive, simple and rapid method has been developed for the determination of SDBS in environmental water samples using a RRS technique. Moreover, the reaction mechanism was discussed.     

Resonance Rayleigh scattering spectral method for the determination of raloxifene using gold nanoparticle as a probe

When gold nanoparticles were being prepared by sodium citrate reduction method, citrate anions self-assembled on the surface of gold nanoparticles to form supermolecular complex anions with negative charges, and protonated raloxifene (Ralo) was positively charged and could bind with the complex anions to form larger aggregates through electrostatic force and hydrophobic effects, which could result in the remarkable enhancement of the resonance Rayleigh scattering intensity (RRS), and the appearance of new RRS spectra. At the same time, the second-order scattering (SOS) and frequency-doubling scattering (FDS) intensities were also enhanced. The maximum wavelengths were located near 370 nm for RRS, 520 nm for SOS, and 350 nm for FDS, respectively. Among them, the RRS method had the highest sensitivity and the detection limit was 5.60 ng mL⁻¹ for Ralo, and its linear range was 0.05-2.37 μg mL⁻¹. A new RRS method for the determination of trace Ralo using gold nanoparticles probe was developed. The optimum conditions of the reaction and influencing factors were investigated. In addition, the reaction mechanism and the reasons for the enhancement of RRS were discussed.     

盐酸氯丙嗪作探针二级散射和倍频散射法测定环境水样中某些阴离子表面活性剂

在pH3.0~5.0的HAc-NaAc缓冲溶液中,盐酸氯丙嗪与十二烷基苯磺酸钠、十二烷基磺酸钠和十二烷基硫酸钠等阴离子表面活性剂反应形成离子缔合物时,仅能引起吸收光谱和荧光光谱的微小变化,但却能导致二级散射(SOS)和倍频散射(FDS)的显著增强。最大SOS峰均在552nm附近,最大FDS峰均在390nm附近。其中SOS法灵敏度更高,它对十二烷基苯磺酸钠、十二烷基硫酸钠和十二烷基磺酸钠的检出限分别为0.047、0.106和0.117mg/L,而其线性范围分别为0.2~12、0.4~15和0.4~20.0mg/L。研究了反应产物的吸收、荧光、SOS和FDS光谱特征、适宜的反应条件及分析化学性质,据此发展了一种用SOS技术灵敏、简便、快速测定阴离子表面活性剂的环境友好型新方法。     

Effects of interaction of folic acid with uranium (VI) and basic triphenylmethane dyes on resonance Rayleigh scattering spectra and their analytical applications

In pH 4.2-4.8 HAc-NaAc buffer solution, folic acid (FA) could react with uranium (VI) to form a 2:1 anionic chelate which further reacted with some basic triphenylmethane dyes (BTPMD) such as Ethyl Violet (EV), Methyl Violet (MV) and Crystal Violet (CV) to form 1:2 ion-association complexes. As a result, not only the absorption spectra were changed, but also the intensities of resonance Rayleigh scattering (RRS) were enhanced greatly and the new RRS spectra were observed. The maximum RRS wavelengths were located at 328 nm for EV system, 325 nm for MV system and 328 nm for CV system. The fading degree (ΔA) and RRS intensities (ΔI) of three systems were different. Under given conditions, the ΔA and ΔI were all directly proportional to the concentration of FA. The linear ranges and the detection limits of RRS methods were 0.0039-5.0 μg mL⁻¹ and 1.2 ng mL⁻¹ for EV system, 0.0073-4.0 μg mL⁻¹ and 2.2 ng mL⁻¹ for MV system, 0.014-3.5 μg mL⁻¹ and 4.7 ng mL⁻¹ for CV system. The RRS methods exhibited higher sensitivity, so they are more suitable for the determination of trace FA. The optimum conditions, the influencing factors and the effects of coexisting substances on the reaction were investigated. The method can be applied to the determination of FA in serum and urine samples with satisfactory results. The structure of the ternary ion-association complex and the reaction mechanism were discussed in this work.     

Spectral study on nuclear fast red-clomifene citrate-sodium dodecyl benzene sulfonate system and its analytical application

The interaction of clomifene citrate with nuclear fast red and sodium dodecyl benzene sulfonate (SDBS) was investigated by absorption and resonance rayleigh scattering (RRS) spectrometry. Clomifene citrate can react with nuclear fast red and sodium dodecyl benzene sulfonate to from a ternary complex, resulting in the enhancement of RRS intensity and the appearance of new RRS peaks. Based on that, a novel method is proposed for the determination of clomifene citrate by RRS technique. On the optimum conditions, there was good linearship between the increments of RRS intensity and the concentration of clomifene citrate in the range of 0.25 to 20.0 μg/mL, with the detection limit of 29 ng/mL. The proposed method was applied to assay the clomifene citrate tablets and capsules in agreement with the method in Chinese Pharmacopeia. In addition, the method was also applied to detect clomifene citrate in serum and urine samples with satisfactory results. The studies indicate that clomifene citrate acts as a bridge to band with nuclear fast red and SDBS by virtue of electrostatic attraction to form ion-association complexes.     

Resonance Rayleigh scattering, frequency doubling scattering and second-order scattering spectra of the heparin-crystal violet system and their analytical application

In pH 6.0-11.2 Britton-Robinson buffer solution, binding of heparin with crystal violet (CV) can result in a significant enhancement of resonance Rayleigh scattering (RRS) and resonance non-linear scattering, such as frequency doubling scattering (FDS) and second-order scattering (SOS). Their maximum scattering wavelengths, λ_ex/λ_em, appear at 492 nm/492 nm for RRS, 984 nm/492 nm for FDS and 492 nm/984 nm for SOS, respectively. The optimum conditions of the reaction, the influencing factors and the relationship between the three scattering intensities and the concentration of heparin have been investigated. New methods for the determination of trace amounts of heparin based on the RRS, FDS and SOS methods have been developed. The methods exhibit high sensitivities, the detection limit for heparin is 2.9 ng ml⁻¹ for the RRS method, 3.5 ng ml⁻¹ for the FDS method and 3.3 ng ml⁻¹ for the SOS method. The methods have good selectivity and were applied to the determination of heparin in heparin sodium injection samples with satisfactory results.     

Study on the resonance Rayleigh scattering spectra of the interactions of palladium (II)-cephalosporins chelates with 4,5-dibromofluorescein and their analytical applications

In pH 2.8-3.8 BR buffer medium, the third generation cephalosporin antibiotics (TGCs) such as ceftazidime (CZD), ceftriaxone (CTRX), cefoperazone (CPZ), and cefotaxime (CFTM) react with palladium(II) (Pd(II)) to form 1:2 yellowish-brown cationic chelates, which further react with 4, 5-dibromofluorescein (DBF) to form 1:3 brown ion-association complexes. As a result, not only the spectra of absorption and fluorescence are changed, but also the resonance Rayleigh scattering (RRS) is enhanced greatly and the new RRS spectra are observed. The four TGCs products have similar spectral characteristics and their maximum RRS wavelengths are all located at 291 nm. The quantitative determination ranges and the detection limits of the four TGCs are 0.0065-1.0 μg mL⁻¹ and 2.0 ng mL⁻¹ for CZD, 0.0070-1.1 μg mL⁻¹ and 2.2 ng mL⁻¹ for CTRX, 0.0090-1.6 μg mL⁻¹ and 2.7 ng mL⁻¹ for CPZ, and 0.014-2.2 μg mL⁻¹ and 4.2 ng mL⁻¹ for CFTM, respectively. The optimum conditions of the reactions and the effects of foreign substances are investigated, and the composition of ion-association complexes is discussed also. Based on the ion-association reaction, a highly sensitive, simple and rapid method has been proposed to the determination of TGCs.     

Study on the resonance Rayleigh scattering, second-order scattering and frequency doubling scattering spectra of the interactions of palladium(II)-ceftriaxone chelate with anionic surfactants and their analytical applications

In pH 1.8-2.9 Britton-Robinson (BR) buffer medium, ceftriaxone (CTRX) can react with palladium(II) (Pd(II)) to form 1:2 cationic chelate, which can further react with anionic surfactants (AS) such as sodium lauryl sulfonate (SLS), sodium dodecyl sulfate (SDS) and sodium dodecylbenzene sulfonate (SDBS) to form 1:3 ion-association complexes. As a result, the resonance Rayleigh scattering (RRS), second-order scattering (SOS) and frequency doubling scattering (FDS) were enhanced greatly. The maximum RRS, SOS and FDS wavelengths of three ion-association complexes were located at 335 nm, 560 nm and 390 nm, respectively. The increments of scattering intensity (DeltaI) were directly proportional to the concentrations of CTRX in certain ranges. The detection limits (3sigma) of CTRX for SLS, SDBS and SDS systems were 1.8 ng ml(-1), 2.3 ng ml(-1) and 2.3 ng ml(-1) (RRS method), 4.9 ng ml(-1), 7.4 ng ml(-1) and 4.7 ng ml(-1) (SOS method) and 6.8 ng ml(-1), 7.3 ng ml(-1) and 9.1 ng ml(-1) (FDS method), separately. The sensitivity of RRS method was higher than those of SOS and FDS methods. The optimum conditions of RRS method and the influence factors were investigated, and the composition of ion-association complexes and the reaction mechanism were discussed also. The effects of foreign substances were tested and it showed that the method has a good selectivity. Based on the ion-association reaction, the sensitive, simple and rapid methods for the determination of CTRX have been developed.     

盐酸氯丙嗪作探针共振瑞利散射法测定环境水样中某些阴离子表面活性剂

在pH 3.0～5.0的HAc-NaAc缓冲溶液中, 盐酸氯丙嗪与十二烷基苯磺酸钠(SDBS)、十二烷基硫酸钠(SDS)和十二烷基磺酸钠(SLS)等阴离子表面活性剂反应形成离子缔合物时, 能导致共振瑞利散射(RRS)的显著增强并产生新的RRS光谱, 最大RRS峰分别位于277, 369和277 nm处, 方法对SDBS, SDS和SLS的检出限分别为0.018, 0.046和0.200 μg/mL, 其线性范围分别为0.09~10.0, 0.15~15.0 和0.67~12.5 μg/mL. 研究了适宜的反应条件及分析化学性质, 提出了一种用RRS技术灵敏、简便并快速测定阴离子表面活性剂的新方法.     

钴(II)-2-(5-溴-2-吡啶偶氮)-5-二乙氨基酚体系共振瑞利散射法测定环境 水样中十二烷基苯磺酸钠

在pH 1.8～3.0的Britton-Robinson (BR)缓冲溶液中, 钴(II)与2-(5-溴-2-吡啶偶氮)-5-二乙氨基酚(5-Br-PADAP)(HL)反应形成紫红色螯合阳离子, 此时仅能引起吸收光谱的变化, 不能导致共振瑞利散射(RRS)的增强. 当钴(II)-5-Br-PADAP螯合阳离子与阴离子表面活性剂十二烷基苯磺酸钠(SDBS)、十二烷基磺酸钠(SLS)和十二烷基硫酸钠(SDS)作用时, 仅能与SDBS进一步反应形成三元离子缔合物并引起RRS的显著增强, 而不与SDS和SLS产生类似反应. 离子缔合物的RRS峰分别位于306, 370和650 nm处, 在一定范围内RRS增强(ΔI)与SDBS浓度成正比, 当用650 nm处测量时, 其检出限为0.043 μg•mL－1, 线性范围为0.14～6.0 μg•mL－1. 文中研究了反应产物的RRS光谱特征, 适宜的反应条件及分析化学性质, 据此发展了一种在一定量SDS和SLS等阴离子表面活性剂存在下选择性测定SDBS的新方法, 方法灵敏、简便、快速,用于天然水和污水中SDBS的测定, 获得满意结果. 文中还对反应机理进行了讨论.     

Resonance Rayleigh scattering measurement of aminoglycoside antibiotics with Evans Blue.

In a weak acid medium, some aminoglycoside antibiotics, such as kanamycin (KANA), gentamicin (GEN), tobramycin (TOB) and neomycin (NEO), or acid bisazo dye Evans Blue (EB) can only produce very weak resonance Rayleigh scattering (RRS) signals. However, when two agents react with each other to form ion-association complexes, the RRS intensity can be greatly enhanced and a new RRS spectrum with a significant enhancement of the RRS intensity in the wavelength range from 350 nm to 600 nm can be observed. The maximum scattering peak is at 570 nm. There is a linear relationship between the RRS intensity and the antibiotic concentration in the range of 0.01-6.0 microg mL(-1) at 570 nm. This RRS method for the determination of aminoglycoside antibiotics at trace-amount levels has been developed. The detection limits (3sigma) of the four antibiotics, whose order of sensitivity from high to low ranks as KANA > NEO > TOB > GEN, are 5.2-6.9 ng mL(-1). This method has good selectivity and has been successfully applied to the quick determination of antibiotics not only for injections and ear drops, but for clinic serum samples as well. In addition, the reaction mechanism by using a quantum chemistry method and the influencing factors of the RRS spectra and the enhancement reasons of RRS have been discussed.     

Resonance Rayleigh scattering spectra for studying the interaction of aminoglycoside antibiotics with pontamine sky blue and their analytical applications

In a weakly acid medium, some aminoglycoside antibiotics, such as kanamycin (KANA), gentamicin (GEN), tobramycin (TOB), and neomycin (NEO), or acid bisazo dye pontamine sky blue (PSB) can only produce very weak resonance Rayleigh scattering (RRS) signals. However, when the two agents react with each other to form the ion association complexes, the RRS intensity can be enhanced greatly and a new RRS spectrum and a significant enhancement of the RRS intensity in the wavelength range 350-600 nm can be observed. The maximum scattering peak is at 580 nm. There is a linear relationship between the RRS intensity and the antibiotic concentration in the range 0.01-6.0 microg mL(-1) at 580 nm. This RRS method has therefore been developed for the determination of trace levels of aminoglycoside antibiotics. The detection limits (3 sigma) of the four antibiotics, whose order of sensitivity is KANA>NEO>TOB>GEN, are 5.8-6.9 ng mL(-1). This method has a good selectivity and has been successfully applied to the quick determination of antibiotics not only for injections and ear drops, but clinic serum samples as well. In addition, quantum chemistry-based analysis of the reaction mechanism, the factors influencing the RRS spectra, and the reasons for the enhancement of RRS are discussed.     

Resonance Rayleigh scattering determination of trace amounts of Al in natural waters and biological samples based on the formation of an Al(III)-morin-surfactant complex

A novel method for the determination of trace amounts of Al(III) based on resonance Rayleigh scattering (RRS) has been developed. In the presence of some surfactants, Al(III) can react with morin and form an Al(III)-morin-surfactant complex, which results in the enhancement of RRS intensity and the appearance of the corresponding RRS spectral characteristics. Their maximum scatter peaks are at 476 nm for the cetyltrimethylammonium bromide (CTAB) system, 489 nm for the cetylpyridinium chloride (CPC) system, 474 nm for the Triton X-100 system, and 473 nm for the Tween-20 system. The enhanced RRS intensity is directly proportional to the concentration of Al(III). The detection limits are in the range of (0.50-1.2)×10⁻⁷ mol l⁻¹ depending on the surfactant. The characteristics of RRS spectra of the complexes, the optimum conditions of these reactions and the influencing factors have been investigated. The method has high selectivity, and was successfully applied to the determination of Al(III) in natural and biological samples. Furthermore, according to different complexation capacity of Al(III)-morin-CTAB system under two pH conditions, speciation analysis of Al(III) in natural waters was explored. The labile monomeric Al fraction (mainly inorganic Al, Al_i) is determined at acidic pH and the total monomeric Al fraction (Al_a) is determined at alkaline pH. The results are in agreement with those obtained by Driscoll’s 8-hydroxyquinoline extraction-ion exchange method.     

铁氰化钾高灵敏共振瑞利散射法测定青霉素类抗生素

在稀HCl介质中,K3[Fe(CN)6]与阿莫西林(AMO)、氨苄西林(AMP)、氯唑西林钠(CLO)、羧苄西林钠(CAR)和青霉素钠(BEN)等抗生素药物在加热条件下反应生成结合产物,会导致溶液的共振瑞利散射(RRS)强度急剧增强,并产生新的RRS光谱,5种反应产物的最大散射峰均位于330 nm附近。在一定的浓度范围内,不同的反应体系散射强度(∆I)与药物浓度成正比,反应具有很高的灵敏度,K3[Fe(CN)6]对5种药物的检出限分别在4.61至5.62 ng·mL-1之间。本文研究了RRS的光谱特征和适当的反应条件,并讨论了反应机理和散射增强的原因,还考察了共存物质的影响,表明方法具有较好的选择性,可用于胶囊、片剂和人血清及尿液中青霉素类药物的测定。     

曲利本红与氨基糖苷类抗生素相互作用的共振瑞利散射光谱及其分析应用

在弱酸条件下，酸性双偶氮染料曲利本红（TR）或硫酸卡那霉（KANA）、硫酸 新霉素（NEO）、硫酸庆大霉素（GEN）和硫酸妥布霉素（TOB）等氨基糖苷类抗生 素的各自共振瑞利散射（RRS）十分微弱，但两者相互作用形成离子缔合物时能使 RRS急剧提高并产生新的RRS光谱，在400～535nm之间有一个强的散射带，最大散射 峰位于400nm处，在0.013～6.0μg·mL~(-1)范围内RRS强度与抗生素浓度成正比， 可用于氨基糖苷类抗生素的测定，对不同抗生素的检出限（3σ）在12.9～17.6ng ·mL~(-1)之间，其灵敏度的顺序是KANA＞NEO＞TOB＞GEN,方法有较好的选择性， 可用于市售抗生素注射液或滴耳液中药物含量和临床血药浓度的快速测定，中还用 量子化学方法对反应机理进行探讨，并讨论了的RRS光谱特性的影响因素和RRS增强 的原因。     

铜（Ⅱ)-氟喹诺酮类抗生素螯合物与虎红体系的吸收和共振瑞利散射光谱及其分析应用研究

在pH 4.2~4.8的B-R缓冲介质中,莫西沙星（MXFX)和加替沙星（GTF)等氟喹诺酮类抗生素（FLQs)能与铜（Ⅱ)形成螯合阳离子,进一步与虎红（Tf)阴离子通过静电引力和疏水作用形成FLQs∶Cu（Ⅱ)∶Tf为1∶1∶1的离子缔合物,体系反应导致共振瑞利散射（RRS)显著增强并出现新的RRS光谱。两种药物的反应产物具有相似的光谱特征,最大RRS峰位于373 nm处,并在590 nm处有1个较小的散射峰。在373 nm处一定浓度的抗生素与散射增强（ΔI)成正比,MXFX和GTF的线性范围分别为0.031~7.8 mg/L和0.029~9.0 mg/L。据此建立了测定氟喹诺酮类药物的新方法,方法用于胶囊和人尿液中FLQs的测定并取得满意结果。同时对反应机理及RRS增强原因进行了讨论。     

Special characteristics of fluorescence and resonance Rayleigh scattering for cadmium telluride nanocrystal aqueous solution and its interactions with aminoglycoside antibiotics

CdTe nanocrystals (CdTe NCs) were achieved by reaction of CdCl₂ with KHTe solution and were capped with sodium mercaptoacetate. The product was detected by transmission electron microscopy (TEM), high-resolution transmission electron microscopy (HRTEM), energy dispersive spectroscopy (EDS), fluorescence spectra, ultraviolet-visible spectra and X-ray diffraction (XRD). The CdTe NCs are of cubic structure and the average size is about 5 nm. The fluorescence quantum yield of CdTe NCs aqueous solution increased from 37% to 97% after 20 d under room light. The maximum λ _em of fluorescence changed from 543 nm to 510 nm and the blue shift was 33 nm. CdTe NCs aqueous solution can be steady for at least 10 months at 4 in° a refrigerator. The resonance Rayleigh scattering (RRS) of CdTe NCs in the aqueous solution was investigated. The maximum scattering peak was located at about 554 nm. The interactions of CdTe NCs with amikacin sulfate (AS) and micronomicin sulfate (MS) were investigated respectively. The effects of AS and MS on fluorescence and RRS of CdTe NCs were analyzed. It was found that AS and MS quenched the photoluminescence of CdTe NCs and enhanced RRS of CdTe NCs. Under optimum conditions, there are linear relationships between quenching intensity (F ₀-F), intensity of RRS (I-I ₀) and concentration of AS and MS. The detection limits (3б) of AS and MS are respectively 3.4 ng·mL⁻¹ and 2.6 ng·mL⁻¹ by the fluorescence quenching method, and 15.2 ng·mL⁻¹ and 14.0 ng·mL⁻¹ by the RRS method. The methods have high sensitivity, thus CdTe NCs may be used as fluorescence probes and RRS probes for the detection of aminoglycoside antibiotics. Supported by the National Natural Science Foundation of China (Grant No. 20475045)     

某些蒽环类抗癌药物与刚果红相互作用的吸收、荧光和共振瑞利散射光谱研究

在pH 2.5左右的酸性介质中, 刚果红与表柔比星、柔红霉素和米托蒽醌等蒽环类抗生素反应形成离子缔合物时, 仅能引起吸收光谱和荧光光谱的微小变化, 但却能导致共振瑞利散射(RRS)的显著增强并产生新的RRS光谱, 与此同时也观察到二级散射(SOS)和倍频散射(FDS)的增强. 最大RRS峰位于370 nm附近, 并在280 nm附近有另一散射峰. 而它们的SOS峰均在530 nm附近, 最大FDS峰均位于353 nm处. 其中RRS法灵敏度最高, 它对表柔比星、柔红霉素和米托蒽醌的检出限分别为0.054, 0.058和0.033 μg/mL, 而其线性范围分别为0.05～12.0, 0.05～12.0和0.04～7.5 μg/mL. 文中研究了反应产物的吸收、荧光和RRS光谱特征, 适宜的反应条件及分析化学性质, 据此发展了一种用RRS技术灵敏、简便、快速测定蒽环类抗癌药物的新方法.