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吲哚二碳菁染料的合成 总被引:2,自引:0,他引:2
合成了五种N-烷基吲哚二碳菁染料,通过质谱、核磁共振氢谱及红外光谱确证了化合物结构。测定了化合物在溶液中的紫外吸收光谱和溶解度,讨论了溶解度、熔点与染料分子结构的关系。 相似文献
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新型水溶性荧光标示剂吲哚方酸菁染料的合成及光谱性能 总被引:1,自引:0,他引:1
用方酸与不同的N烷基取代吲哚啉季铵盐缩合制备了一系列对称的水溶性方酸菁染料. 通过核磁共振氢谱和质谱对合成的染料结构进行了表征, 研究了它们在不同溶剂中的吸收和发射光谱. 结果表明, 随着溶剂极性的增大, 染料的吸收光谱发生蓝移, 表现为负向溶剂化效应, 在极性溶剂中的荧光量子产率比在水中的大. 考察了N位取代基对染料水溶液光稳定性的影响, 结果表明在吲哚环N原子上引入较大的苄基有助于提高光稳定性, 且随着苄基上取代基吸电子能力的增强, 染料的光稳定性增强. 相似文献
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在吡啶中, 吲哚啉碘盐与原乙酸三乙酯缩合合成了一些新的吲哚碳菁染料.根据紫外-可见光谱, 荧光光谱讨论了它们的取代基和光谱特征的关系. 相似文献
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以蛋白质或多肽修饰的吲哚类菁染料Cy3为内核, 采用实验条件简单的油包水反相微乳液方法成核, 通过正硅酸乙酯水解形成的网状二氧化硅包壳的方法制备吲哚类菁染料Cy3嵌入的核壳荧光纳米颗粒. 考察了以不同等电点的蛋白质和多肽修饰的Cy3为内核材料对吲哚类菁染料Cy3嵌入的核壳荧光纳米颗粒制备的影响. 结果表明, 分别采用人免疫球蛋白(IgG)或多聚赖氨酸修饰的Cy3为内核材料, 都能制备荧光强度高、荧光稳定性强和染料泄漏极少的Cy3嵌入的核壳荧光纳米颗粒. 进一步对Cy3嵌入的核壳荧光纳米颗粒进行了表征, 并将基于这一新型的荧光纳米颗粒建立起来的生物标记方法初步应用于流感病毒DNA的检测, 其检测线性范围为3.18×10-10~1.27×10-9 mol/L, 检测下限为3.51×10-10 mol/L, 相关系数r为0.986 5. 相似文献
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将(未)确定的1-(3'-磺酸丙基)2,3,3-三甲基吲哚啉内盐与原乙酸三乙酯在吡啶或酸酐中回流合成了五个氮原子上带有磺酸丙基的取代吲哚碳菁染料. 这些染料和杂环氮原子上带有烷基取代的吲哚碳菁相比, 它们具有较高的熔点, 在极性溶剂中具有较高的溶解度. 这些染料的最大吸收值在540~580nm之间, 并具有较高的消光系数(1~2×10^5L·mol·cm^-^1) . 相似文献
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含有吲哚杂环的菁染料广泛地应用于各个领域,可作为荧光探针、医用检验试剂、织物染料、激光染料,更普遍的是作为光记录介质,感光材料里的添加剂. 相似文献
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With the development of life science,there is a grow ing requirement for high sensit ive detect ion technolog ies in analysis.Fluorescence label is a convenient method.Indocyanines with larger ex tinction coefficients(105L/mol cm),good fluorescent proper ties and fluorescence enhancement combining with biomass have been used as a new generation of fluorescent label compounds in biological analysis.How ever,photostability of these dyes has been a problem.In this paper the substituents on the nitro gen atoms in the heterocyclic rings of these indo-cyanines were designed as p-carbox ybenzyl groups,and ser ies novel straight chain polymet hine indocyanines w ere synthesized.Introduction of rigid rings into the methine chain resulted in other series novel 3H-indocya-nines. 相似文献
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Two fluorescent indocyanine dyes containing at least one p-carboxybenzyl group on the nitrogen atoms in the heterocyclic rings were designed and synthesized.Their absorption maxima were 549nm and 551nm in water respectively.They had good water solubility and photostability. 相似文献
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Canjia Zhai Dr. Cynthia L. Schreiber Sasha Padilla-Coley Dr. Allen G. Oliver Prof. Bradley D. Smith 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2020,132(52):23948-23955
A general synthetic method creates a new class of covalently connected, self-threaded, fluorescent molecular probes with figure-eight topology, an encapsulated deep-red fluorophore, and two peripheral peptide loops. The globular molecular shape and rigidified peptide loops enhance imaging performance by promoting water solubility, eliminating probe self-aggregation, and increasing probe stability. Moreover, the peptide loops determine the affinity and selectivity for targets within complex biological samples such as cell culture, tissue histology slices, or living subjects. For example, a probe with cell-penetrating peptide loops targets the surface of cell plasma membranes, whereas, a probe with bone-targeting peptide loops selectively stains the skeleton within a living mouse. The unique combination of bright deep-red fluorescence, high stability, and predictable peptide-based targeting is ideal for photon intense fluorescence microscopy and biological imaging. 相似文献
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Amandeep Kaur Dr. Jacek L. Kolanowski Dr. Elizabeth J. New 《Angewandte Chemie (International ed. in English)》2016,55(5):1602-1613
The redox chemistry of the cell is key to its function and health, and the development of chemical tools to study redox biology is important. While fluxes in oxidative state are essential for healthy cell function, a chronically elevated oxidative capacity is linked to disease. It is therefore essential that probes of biological redox states distinguish between these two conditions by the reversible sensing of changes over time. In this review, we discuss the current progress towards such probes, and identify key directions for future research in this nascent field of vital biological interest. 相似文献
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以阿拉伯糖和磷酸酪蛋白肽进行水热反应,制备水溶性多色荧光碳点,利用透射电子显微镜(TEM)、紫外吸收光谱(UV)、荧光光谱(FL)、红外光谱(FTIR)和X射线衍射(XRD)等对所制备碳点的粒径大小、吸收光谱、发光性质、表面基团等进行表征,并考察了其性能和对不同金属离子的识别作用。结果表明:制备的荧光碳点平均粒径为4.62 nm,其紫外最大吸收波长为281 nm,XRD峰值约为21°,可在紫外灯下发出明亮的荧光,最大发射波长为414 nm,且呈荧光多元发射。红外光谱分析表明存在—COOH,—NH2和—OH基团。该荧光碳点具有良好的性能,且对Cu2+和Fe3+有较强的选择性识别作用,其原因可能是荧光碳点的聚合导致粒径增大从而使荧光强度减弱。该碳点有望作为荧光探针用于检测分析和生物成像等领域。 相似文献
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采用傅里叶变换红外光谱、电镜/X射线能谱和原子吸收光谱等现代仪器测试方法对一种水溶性涂料的化学成分进行检测分析,对材料的红外光谱特征进行解析,综合定性和定量分析结果,确定出该涂料的各种组份及其配比. 相似文献
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Dr. Elodie Heyer Pauline Lory Dr. Jérôme Leprince Dr. Mathieu Moreau Prof. Anthony Romieu Prof. Massimo Guardigli Prof. Aldo Roda Dr. Raymond Ziessel 《Angewandte Chemie (International ed. in English)》2015,54(10):2995-2999
The preparation of highly water‐soluble and strongly fluorescent diketopyrrolopyrrole (DPP) dyes using an unusual taurine‐like sulfonated linker has been achieved. Exchanging a phenyl for a thienyl substituent shifts the emission wavelength to near λ=600 nm. The free carboxylic acid group present in these new derivatives was readily activated and the dyes were subsequently covalently linked to a model protein (bovine serum albumin; BSA). The bioconjugates were characterized by electronic absorption, fluorescence spectroscopy and MALDI‐TOF mass spectrometry, thus enabling precise determination of the labeling density (ratio DPP/BSA about 3 to 8). Outstanding values of fluorescence quantum yield (30 % to 59 %) for these bioconjugates are obtained. The photostability of these DPP dyes is considerably greater than that of fluorescein under the same irradiation conditions. Remarkably low detection limits between 80 and 300 molecules/μm2 were found for the BSA bioconjugates by fluorescence imaging with a epifluorescence microscope. 相似文献
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In addition to being the energy powerhouse of the cell, mitochondria are an important source of reactive oxygen species (ROS) during the process of molecular oxygen metabolism. Mitochondrial ROS are closely associated with normal physiological functions as well as human diseases, and participate in cell signaling, nucleic acid and protein damage, and oxidative stress induction. However, the complicated interplay between mitochondrial ROS and the cellular pathological state has not been fully elucidated. It is expected that research on the mitochondrial ROS undertaking in the molecular pathogenesis of human diseases would benefit from development of efficient tools for the detection of these ROS. In recent years, an increasing number of fluorescent probes for mitochondrial ROS with high sensitivity and selectivity have been developed. Here, we present a review of the recent advances in small molecular fluorescent probes for selective detection of ROS inside the mitochondria. In this review, the design, synthesis, characteristics, and applications of the published fluorescent probes for mitochondrial ROS are discussed in detail. 相似文献