QUELQUES OBSERVATIONS SUR LE DÈVELOPPEMENT DES CULTURES IN VITRO DE MOELLE DE TABAC EN PRÉSENCE D'AUXINE ET DE KINÉTINE, EN ÉCLAIRE-MENT RELATIVEMENT MONOCHROMATlQUE

Abstract— In a previous publication we have presented some results on tobacco pith tissue cultures grown in relatively monochromatic light (around 4350,5250, 6600 and 8000 Å).
This paper points out specially that cultures subjected to a continuous blue light period of 12 days only, followed by a darkness period of 29 days, grew no more than cultures exposed to the same radiations during the 41-day period of the experiment.
The growth was only 67 per cent of that of tissues grown in the darkness.
The morphologic and histologic observations showed the same differences between the cultures grown in blue light conditions and the cultures grown in the other light conditions, as also reported elsewhere.
Observation of bud developement on stem segment cultures in vitro of willow, grown under these relatively monochromatic light conditions, together with these histologic studies, leads us to believe in a possible gibberelline action in blue light.     

INFLUENCE DE LA LUMIÈRE SUR LES MOUVEMENTS DE NUTATION DES PLANTULES

Abstract— Red light can promote (a) the straightening of the curvature ( Phaseolus vulgaris, Pisum sativum ) (b) the formation of a loop ( Phacelia tanacetifolia and Picea abies ) or (c) the induction of a curvature which does not occur in the darkness ( Lactuca sativa ). Thesc red-light induced movements could be reversed by following each brief red exposure with short irradiation by far-red light. These reactions are controlled by the phytochrome system.
On the other hand, exposure to far-red and blue light during a long period can induce other movements:
(a) The light re-opens the hook which has been closed by red radiation ( Lactuca sativa ).
(b) The light induces the straightening of the dark-induced curvature ( Phacelia tanacetifolia ). These facts may be due to an unknown property of phytochrome or to the action of another photoreceptor.     

ACTION DE LA LUMIÈRE SUR LE DÉVELOPPEMENT DES TISSUS DE FEUILLES D'ENDIVE CULTIVÉS IN VITRO

Abstract— Light is not necessary for the formation of buds by tissues of chicory roots. However, in darkness, their number is reduced and the development of leaves is slowed down considerably. Absence of light also reduces the proliferative capacity of leaf explants as well as the number of buds formed on them. The number of buds is greater and their growth more rapid for longer periods of illumination. Also, the photoperiod conditions the formation of flowers in vitro .     

CINETIQUE DES ECHANGES D'OXYGENE ET DE LA FLUORESCENCE DES CHLOROPLASTES ISOLES

Abstract Oxygen evolution and fluorescence have been studied with isolated chloroplasts illuminated, in the absence of Hill reagents, by flashes or continuous light. As in whole cells, at least two substances are involved in the primary process leading to the oxygen evolution. The first, called E, probably is the photochemical “complex” of System II. After a long period of darkness, E is not active. It is activated in two steps. Step one is a photochemical reaction, induced by a quantum of light absorbed by pigment-system II, which results in the production of E in a reduced state. Step two is a dark oxidation of the reduced E by the second substance, A. The oxidized E can then enter the normal photochemical cycle of system II. Reduced E might alternatively be oxidized by oxygen, this reaction being responsible for a very rapid and brief light-induced oxygen uptake. Substance A is measured by the oxygen burst and is present in the chloroplasts at the approximate ratio of 1 molecule for 70 molecules of total chlorophyll while E is at the ratio of about 1/2800. This gives a E over A value of 1/35 which is much smaller than the one found in whoe cells (ca. 1/10). This independent behavior of E and A suggests that chloroplast extraction destroys some photochemical centers without having a direct impact on A, which might diffuse from one center to another. Besides the brief light-induced oxygen uptake above mentioned, there is another one which is related to System I functioning. The kinetics of the oxygen evolution and of the fluorescence have been compared. During the activation process of the oxygen evolving ability, rate of oxygen evolution and fluorescence yield increase in a parallel way. After the maximum velocity of the oxygen burst is reached (i.e. after activation), the fluorescence yield keeps growing up until the steady-state is attained (with an intermediary plateau), whereas the rate of oxygen emission slows down. The time-course curves of fluorescence obtained with inactivated or activated chloroplasts are essentially different in that the initial yield is higher in the latter case.     

Effects of Selenium and Light Wavelengths on Liquid Culture of <Emphasis Type="Italic">Cordyceps militaris</Emphasis> Link

To investigate the effects of selenium and light wavelengths on the growth of liquid-cultured Cordyceps militaris and the main active components’ accumulation, culture conditions as selenium selenite concentrations and light of different wavelengths were studied. The results are: adenosine accumulation proved to be significantly selenium dependent (R ² = 0.9403) and cordycepin contents were determined to be not significantly selenium dependent (R ² = 0.3845) but significantly enhanced by selenium except for 20 ppm; there were significant differences in cordycepin contents, adenosine contents, and mycelium growth caused by light wavelengths: cordycepin, blue light > pink light > daylight, darkness, red light; adenosine, red light > pink light, darkness, daylight, blue light; and mycelium growth, red light > pink light, darkness, daylight > blue light. In conclusion, light wavelength had a significant influence on production of mycelia, adenosine, and cordycepin, so lightening wavelength should be changed according to target products in the liquid culture of C. militaris.     

PHOTOBIOLOGIE DU MÉSOCOTYLE D''AVOINE-III. DOUBLE EFFET DE LA LUMIÈRE BLEUE

Abstract— Using excise sections of oat first-internodes, a dual effect of blue light can be demonstrated on elongation when the sections are first irradiated in distilled H₂O, then incubated with gibberellic acid (GA). At low light energies (230 ergs/cm² per sec, for 2 min), a pretreatment with blue light enhances the GA effect above the elongation it can produce in the dark. At high energies (650 ergs/cm²/sec for 45 min), the same wavelengths cause an inhibition of the GA-induced elongation. An action spectrum for the two effects show a maximum near 435 mμ in both cases. Neither light effect is visible when indoyl-3-acetic acid is used instead of GA. Several physiological effects distinguish the two blue effects. The promotive effect is most marked in the young regions of the mesocotyl, whereas the maximum inhibitive effect is located in slightly older tissues. Time-course experiments showed that the promotive effect is partly due to an extension of the duration of elongation. The inhibitory effect is only temporary and vanishes about 30 hr after the beginning of the experiment. The promotive effect of blue light resembles the effect of far-red light, but the former can be observed with gibberellins A₂, A₄, A₅, A₆ and A₇ which are practically inactive after an irradiation with far-red light. The inhibitory effect of blue light is different from the red-light effect as shown by the time-course experiments.     

SOME EFFECTS OF LIGHT ON LEAF GROWTH IN PISUM SATIVUM AND TROPAEOLUM MAJUS

Abstract— The effect of wave-length of light on leaf expansion in Tropaeolum majus 'Double Orange Gleam' and Pisurn sativum 'Meteor' has been studied. In both species leaf growth is strongly promoted by light.
Increasing the daily duration of exposure to light increased leaf expansion in Tropueolum in both blue and red wave-bands over a range of light intensities. In Pisum a similar effect of ail increase in duration of irradiation was found over the whole range of intensities used for blue light but only at the highest intensity for red light: at the two lower intensities in red an increase in duration of the light treatment beyond 1 hr did not increase leaf expansion.
In both species a period of 4 hr of blue followed by 4 hr of red promoted leaf growth more than 4 hr of red followed by 4 hr of blue. The effect of a 4 hr period of red light was largely prevented when it was followed by far-red; the effect of 4 hr of blue light, on the other hand, was not affected by subsequent exposure to far-red.
It is concluded that leaf growth in Pisum and Tropaeolum is dependent not only on the 'low-energy' red/far-red reversible reaction but also on one or more 'high-enerFy' photo-reactions.     

ACTION DE LA QUANTITÉ JOURNALIÈRE DE LUMIÈRE SUR LA FLORAISON, LE POIDS SEC ET LA TENEUR EN EAU DE QUELQUES PLANTES DE JOURS COURTS ET DE JOURS LONGS

Abstract— Experiments were carried on in the Liège Phytotron for two years to discover what reactions could be obtained when both short-day and long-day plants were placed in the same light energy per day (light intensity × daily light duration in various combinations) and receiving respectively 8 or 16 hr light per day. It was observed that both types of plants give parallel dry weight curves in both long-day and short-day, but this happens only during the vegetative state following germination.
Differences occur in those dry weight curves in short-day plants and also in long-day plants when cultivated under inductive photoperiodic light and induced to flower.
In these suitable light conditions, their dry weight increased compared with that of plants left in a non-inductive photoperiodic fight.
The evolution of tissue water content (stems, leaves, flowers), expressed as water % of the dry weight, is also interesting to observe in the same experimental environments. This evolution plays an important physiological part in flower formation, as has been shown before by R. Bouillenne.
Both types of plants cultivated in their respective non-inductive light point out a very simple curve of water content. First a rapid increase of water into the tissues during the first 10 days after germination; then from this maximum, the curve goes down regularly and slowly till a constant low level.
However, in inductive light exposure, water content percentage curves are really different for both types of species. Considering the same daily value of light energy given in each case, it is possible to conclude that the induction of flower does not depend on the daily light quantity but on something else which is connected with the photoperiodism.
These fluctuations of the water content will be investigated.     

DIURNAL MODULATION OF PHOTOTROPIC RESPONSE BY TEMPERATURE AND LIGHT IN Chenopodium rubrum L. AS RELATED TO STEM EXTENSION RATE ANDARGININE DECARBOXYLASE ACTIVITY*

Abstract— Seedlings of Chenopodium rubrum were grown under 12: 12 h light-dark cycles with the light period at 32.5°C and darkness at 10°C (“normal” conditions) or with light at 10°C and darkness at 32.5^CC (“inverse” conditions). When grown under unilateral white light with the photo-thermoperiodic conditions described above, inverse seedlings did not show phototropic curvature while normal seedlings did. Regardless of temperature regime used, plants irradiated with unilateral blue light (456 nm) at 10°C failed to curve on subsequent incubation at 32.5°C. If the phototropic stimulus was given at 32.5°C followed again by 32.5°C in darkness, phototropic curvature was observed. The results with monochromatic blue light thus explain the lack of phototropic response of “inverse” seedlings with the unilateral light period given at 10°C. “Inverse “seedlings showed a sharp rhythm in their capacity to respond to phototropic stimulation at 32.5°C. The greatest capacity to respond came when the stem extension rate was actually zero. The differential growth accompanying phototropic curvature thus seems to be different from the growth responsible for uniform stem elongation. The curvature may result from differential turgor changes as in the case of sun tracking of leaves. “Inverse seedlings” showed clear signs of stress and displayed a rhythm in arginine decarboxylase, an enzyme thought to be stress-related, with maxima during the low temperature light periods. It is concluded that the phototropic perception transduction chain is modified by the inverse conditions somewhere before the final growth-turgor steps, since they exhibit geotropic sensitivity.     

AGREGATION DES CHLOROPHYLLES IN VITRO-II. PHOTODECOLORATION DE LA CHLOROPHYLLE a ADSORBEE SUR DES PARTICULES DE LIPOPROTEINES EXTRAITES DU LAIT

Abstract— Chlorophyll a (chl a ) adsorbed on milk proteins or lipoproteins has absorption maxima at 437 ± 1 nm and 671 ± 1 nm, whatever its concentration. A 750-nm-absorbing form appears when (chl/proteins) > 2 × 10^-2 mg chl/mg proteins, in the case of lipoproteins; or 4.5 × 10^-3 mg chl/mg proteins, in the case of proteins. In both cases, the apparent molar extinction coefficient at 671 nm is the same (3 × 10⁴ liter mole^-1 cm^-1) at the highest (chl/proteins) ratio for which no 750 nm absorbancy appears. It is shown that adsorbed chl a undergoes irreversible (in the present conditions) photo-oxidation by light in the presence of oxygen. The reaction is second order, with similar rate constants for chl a adsorbed to proteins or to lipoproteins.     

PHOTOCONTROL OF HYPOCOTYL ELONGATION IN DE-ETIOLATED Cucumis sativus L. RAPID RESPONSES TO BLUE LIGHT

Abstract— Rapid inhibition of hypocotyl extension of de-etiolated Cucumis sativus L. by blue light is described, and compared with responses to white fluorescent light. Rapid inhibition of hypocotyl growth by blue light via the specific blue light photoreceptor requires a minimum Buence rate. Above this minimum value rapid modulations of growth rate accompany changes in blue light fluence rate. An initial response to blue light, or to a step-up in blue fluence rate takes less than 5 min. A drop from high to low fluence rate blue light (or to darkness) is followed by a recovery of the growth rate after about 20 min. A change from a low fluence rate to darkness elicits a more rapid recovery of growth rate (within 7 min). Similar responses were obtained in seedlings de-etiolated for a few hours and for several days.     

ACTION DE LA LUMIÈRE SUR LA CROISSANCE DES TIGES PRINCIPALES DE L'ASPARAGUS MEDEOLOIDES

Abstract— Studies of the influence of light upon the growth of Asparagus medeoloides main stems show that the stem-length and the time-growth increase when the intensity of illumination decreases. There is no direct proportionality between growth and illumination intensity; a maximum is found at 660 lx.     

RELATIONSHIP BETWEEN LIGHT QUALITY DEPENDENCE AND IRRADIANCE ADAPTATION OF PHOTOSYNTHESIS IN Acetabularia mediterranea*

Characteristic differences in the light intensity curves of photosynthesis after growth of cells of Acetabularia mediterranea Lamour. (A. acetabulum (L.) Silva) in weak and strong white light were similar to those for red and blue light-treated cells, respectively. This indicated that responses to white light quantity and those to light quality might be causally related. Small differences in the thylakoid polypeptide composition of cells grown in high and low intensities of white light were not significant and thus did not help to clarify whether the adaptations to blue or red light, respectively, were the same. When the red to blue-light ratio was varied, keeping the total photon fluence rate constant, the photosynthetic capacity (red light saturated O₂-production) was dependent on blue light irradiance in a logarithmic fashion. The specific influence of red light was not detectable, indicating that only blue light was effective for light irradiance adaptation in Acetabularia. The situation was different, at least for a transient period, when adaptation to light irradiance was allowed to proceed from a low photosynthetic activity after preirradiation of the cells with prolonged red light. The effect of low white light irradiances was pronounced, causing a maximum increase of photosynthetic activity within 3 days. The response to blue light was enhanced as well, and a very low photon irradiance added to continuous red light caused a change of the same order as that produced by high irradiances of blue light alone. This elevated action of low intensity white and blue light is most likely due to increased metabolite supply derived from the degradation of starch enhanced by this light quality. Therefore, photosynthetic effectiveness in Acetabularia is regulated by the irradiance of blue light and by feedback via photosynthetic products.     

MODIFICATION DU TAUX DES NUCLEOTIDES ADENYLIQUES (ATP, ADP et AMP), PAR UN ECLAIREMENT DE LUMIERE ROUGE-CLAIR (660 nm)

Abstract— In oat meSocotyl tissue ( Avena sativa , var. Nuprime), red light (660 nm) modifies the level of adenylic nucleotides. Two types of action have been shown. The first takes place during illumination; there is an increase in the level of ATP and, at the same time, a decrease of ADP. This effect disappears within 5 min after the end of illumination. The second takes place 1–2 hr after illumination when mesocotyl segments are put in growth medium. The ATP level drops in the red-illuminated fragments compared to those kept in the dark. There is no difference in the ADP level under these two conditions.
It is not yet known if this light action is a phytochrome-mediated process.     

A POSSIBLE CONTROL BY PHYTOCHROME and OTHER PHOTORECEPTORS OF PROTEIN ACCUMULATION IN THE GREEN ALGA Ulva rigida

Abstract— The effects of red, blue and green light pulses on total protein accumulation in the green alga Ulva rigida following transfer from a low to high nitrate medium in darkness were examined. Red light pulses prior to transfer to darkness increased protein accumulation by about 55%. Blue and green light pulses also stimulated protein accumulation, but to a lesser extent (40-30% respectively). Stimulation of protein accumulation by red, blue or green light was largely (red light) or partially (blue or green light) reversible by far-red. The role of phytochrome and a red/green photoreversible system in the control of protein synthesis is discussed.     

Light induced synchronization of algal species that divide preferentially in darkness

Abstract— Three algal species (Protosiphon botryoides, Chlorella pyrenoidosa and Chlamy-domonas gymnogama) which divide preferentially at night during natural or simulated day-night conditions are shown to have cell division inhibited by light in the blue region of the visible spectrum (400–500 nm) and stimulated by the remainder of this spectrum (500–730 nm). Synchronous division has been established in cultures of these organisms on a circadian or longer period by alternating fluorescent cool-white light and cool-white with the blue component removed. This procedure is suggested as potentially superior to other methods for synchronizing cultures of algae that prefer, in nature, to undergo cell division at night. Where this procedure does not work it is recommended that an action spectrum for cell division be obtained, since this may provide information for achieving optimal synchrony through the use of other specific wavelength regimes.     

THE PHOTOBIOLOGY OF Paphiopedilum STOMATA: OPENING UNDER BLUE BUT NOT RED LIGHT

Abstract— The responses of stomata from Paphiopedilum harrisianum , Orchidaceae, to light and CO₂ were studied in epidermal peels. Stomatal opening under red light was indistinguishable from that in darkness, whereas blue light promoted opening above dark levels. The ineffectiveness of red light in causing stomatal opening was confirmed in the presence of 100 μ M KCN; average apertures in both darkness and red light were 53% of those measured in the absence of the inhibitor, whereas under blue irradiation, the KCN inhibition was only 30%, with average apertures two-fold of those measured under red light or darkness. Fluence rate response curves under blue light were typical of a single photoreceptor; removal of CO₂ increased aperture values without a significant light-CO₂ interaction. The lack of a stomatal red light response contrasts with results obtained in species with chlorophyllous stomata in which red light consistently causes stomatal opening, and suggests that the previously reported red light responses in stomata from intact Paphiopedilum leaves resulted from indirect effects, such as depletion of intercellular CO₂ by mesophyll photosynthesis. In isolation, Paphiopedilum stomata appear to rely on a blue light photosystem for their responses to light and fail to open under red light because of their lack of guard cell chloroplasts.     

THE EFFECT OF RED AND FAR RED LIGHT ON CAROTENOID AND CHLOROPHYLL FORMATION IN PEA SEEDLINGS

Abstract— Treatment of etiolated pea seedlings with a short exposure to red light caused a stimulation of growth (size and dry wt production) and carotenoid synthesis during the following 48 hr compared with seedlings kept entirely in darkness.The effect is nullified by a following dose of far red light and thus the phenomenon is probably phytochrome-controlled.
Similar treatment with red light one hour before continuous illumination with white light tended to reduce the lag period for chlorophyll synthesis.Again a following dose of far red light reversed this response.     

PHOTOCONTROL OF HYPOCOTYL ELONGATION IN DE-ETIOLATED Cucumis sativus L. A BLUE-LIGHT-INDUCED POST-ILLUMINATION BURST OF GROWTH

Abstract— A rapid transient increase in the growth rate of Cucumis sativus L. seedlings is found to occur after a transition from fluorescent white light to darkness. The post-illumination burst of growth starts after about 20 min of darkness and lasts for 30 to 50 min. The response occurs after long periods of continuous white light, in cycles of white light and darkness, whether the cotyledons are covered or exposed. The post-illumination burst is an expression of the loss of photocontrol of hypocotyl inhibition via the specific blue light photoreceptor because (a) its kinetics resemble those of recovery from blue-light inhibition, and (b) it only occurs on transition from a blue-rich to a blue-poor light environment.     

PARTIAL CHARACTERIZATION and LIGHT-INDUCED REGULATION OF GTP-BINDING PROTEINS IN Lemna paucicostata

Binding of GTP-binding proteins with [³⁵S]GTP7S in the extract containing membrane components of Lemna paucicostata 441 was inhibited by red or far red light by 20 to 25%, but blue light showed no or little effect. The plant used for the preparation of the extract was subjected to single darkness for 8 h, as both red and far red light inhibit flowering. The extract treated with 1% Lubrol was fractionated by gel filtration. Four species of GTP-binding proteins, GL1, GL2, GL3 and GL4 were detected with Km values 3, 7, 80 and 4 n M , respectively. GL1, GL2 and GL3 were ADP-ribosylated by pertussis toxin. The extract activated by [³⁵S]GTP-γS in darkness, under red light or under far red light was treated with 1% Lubrol and subsequent gel filtration of the extracts made it possible to detect GTP-binding protein with a small molecular weight only in an extract labeled in darkness. The reduction in the molecular weight of GTP-binding protein from the larger molecule associated with the binding of [³⁵S]GTPγS was confirmed by rechromatography of the larger molecule activated by [³⁵S]GTPγS in darkness. The binding of GL2 and/or GL3 with [³⁵S]GTPγS was suggested to be inhibited by red or far red light.