Post-infectional changes in the phospholipids of the leaves of a cotton plant variety resistant toVerticillium dahliae

The class and fatty acid compositions of the phospholipids (PLs) of young leaves of healthy, cotton plants of the wilt-resistant variety 175-F and of plants of the same variety infected with the pathogenic fungusVerticillium dahliae Kleb. have been determined. The positional distributions of the fatty acids in the main classes of PLs have been established, and their possible molecular compositions have been calculated. The changes in the composition of the PLs taking place under the influence of infection have been elucidated.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 210–216, March–April, 1997.     

Fatty acid composition of the phospholipids of the vegetative organs of the cotton plant of variety 159-F at the stage of mass fruit formation

The qualitative and quantitative compositions of the fatty acids (FAs) of the individual phospholipids (PLs) of the vegetative organs of the cotton plant of variety 159-F have been studied. In the PLs of the stems there was a wide range of FAs (26), a large proportion of which have retention times in GLC shorter than those of the 16:0 FA. The position distribution of the FAs in the phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositols of the vegetative organs have been established.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 173–176, March–April, 1984.     

Phospholipids of the medium-fiber cotton plant variety 159-F

Summary 1. The qualitative and quantitative compositions of the PLs of an industrial medium-fiber cotton plant of variety 159-F (1972 and 1974 harvests) have been considered. It has been shown that the total PL amounted to 1.76 and 1.85% and consisted of 60–65% PC, 16–18% PI, 6–8% PE, and 10–14% of combined minor components, respectively.2. The distribution of the lipid phosphorus has been studied during the isolation of PLs by selective solvents. The lipid phosphorus amounted to 5.5% of the total phosphorus in the seed kernels.3. The qualitative and quantitative compositions of the fatty acids of the individual lipid fractions and of the individual PCs, PIs, and PEs have been determined. The position distribution of the fatty acids of the main groups of PLs has been established and a possible molecular composition has been given.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 198–202, March–April, 1977.     

Post-infection changes in lipids of the roots of a cotton plant variety moderately resistant to Verticillium dahliae

A comparative study has been made of the lipid composition of healthy roots of the cotton plant of the Tashkent-1 variety (I) and of the corresponding plants infected withVerticillium dahliae Kleb. (II) grown under field conditions. The action of the factors of infection stress and seasonal and climatic conditions change the lipid metabolism. In lipids (I) and (II) seven classes of neutral lipids (NLs), three classes of glycolipids, and six classes of phospholipids (PLs) were identified and their fatty acid compositions were determined. It was established that in (II) there was a smaller amount of total lipids, NLs, and PLs, and an increased level of polyphenolic pigments, but the level of gossypol pigments with free aldehyde groups was unchanged. In the NLs of (II), as compared with (I), there were more triacylglycerols, sterols, triterpenols, and squalene. Complex changes were observed in the fatty acid composition of the acyl-containing lipids.Institute of the Chemistry of Plant Substances, Uzbek SSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 59–63, January–February, 1989.     

N-acylated phospholipids and lysophospholipids of kenaf seeds

Summary Three minor components have been isolated from the total PLs of kenaf seeds of variety Kuban'-333 by means of column and preparative thin-layer chromatography and they have been characterized by lysophosphatidylcholine and N-acylphosphatidylethanolamine and its lyso analog.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 723–725, November–December, 1976.     

Lipids of Hibiscus cannabinus seeds

The neutral and polar lipids of kenaf seeds of the variety Uzbetskii-1574 from the 1983 crop have been studied. The fatty acid compositions of six acyl-containing classes of neutral lipids have been established for the first time. The acids in the sn-2 positions of the triacylglycerols and of the main classes of phospholipids have been determined. The main triacylglycerol species are those in which the sn-2 position is esterified by linoleic acid.Institute of the Chemistry of Plant Substances of the UzSSR Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 558–564, September–October, 1986.     

Glycolipids of the leaves of a cotton plant of the 175-F variety

The amounts and the quantitative and fatty acid compositions of the classes of lipids from the leaves of a healthy cotton plant of the wilt-resistant variety 175-F and of such a plant artificially infected with Verticillium dahliae have been determined. Quantitative 'changes in individual classes of lipids under the action of infection have been revealed.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 89 14 75. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 801–804, November-December, 1995. Original article submitted May 22, 1995.     

Minor phospholipids of cotton seeds

Summary The minor components of the total PLs of seeds of the cotton plant of variety 108-F have been studied.N-Acylphosphatidylethanolamine and N-acyllysophosphatidylethanolamine have been isolated from cotton seed for the first time and some of their physicochemical properties and their fatty-acid compositions have been studied.Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 580–583, September–October, 1976.     

Efficient and rapid method for extraction of intact phospholipids from sediments combined with molecular structure elucidation using LC–ESI-MS–MS analysis

This paper presents the application of an efficient method for extraction and fractionation of intact phospholipids (PLs) from complex sediment matrices and elucidation of their molecular structure by normal-phase HPLC–ESI-MS–MS. Flow-blending extraction was tested with different solvent mixtures and the best recovery of all PLs classes from the sediment matrix was achieved by using methanol–dichloromethane–buffer, 2:1:0.8. The applied LC–ESI-MS system has linearity of R²=0.98 and a detection limit of 0.5 ng/PL, sufficient for reliable identification of complex mixtures of PLs. MS–MS analyses using a triple-quadrupole mass spectrometer enables detection of individual PL side-chain composition and, hence, characterization of the living organisms contributing to the sedimentary organic material. Parallel GC–MS analysis of the hydrolysed phospholipid fatty acids supports the characterized fatty acid patterns determined from intact PLs. The PL inventory of different investigated lacustrine surface sediments shows predominantly high abundance of phosphatidylglycerols and phosphatidylethanolamines and phosphatidyl-mono- and dimethyl-ethanolamines with fatty acyl side-chains typically known from bacteria. In a sample from Lake Baikal intense signals of bacterial 14:0-acyl-PGs were also identified, for the first time in sediments as far as we are aware.     

Phospholipids of the seeds of experimental varieties of kenaf

Phospholipids of the seeds of kenaf of the variety Opytnyi-1931 are similar with respect to their set of components and the qualitative composition of the fatty acids of homogeneous classes to the phospholipids of the variety Opytnyi-1972, but differ with respect to the amounts of individual acids. The fatty-acid composition of the lysophosphatidylcholines and lysophosphatidylinositols of experimental varieties of kenaf seeds have been studied for the first time.Institute of the Chemistry of Plant Substances of the Academy of Sciences of the Uzbekh SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 2, pp. 158–160, March–April, 1986.     

Effect of a wilt infection on the neutral lipids of cotton leaves

The composition of the neutral lipids of leaves of a cotton plant of the wilt-resistant variety 175-F, the set of components in the fatty acid composition of the lipid classes, and the partial structures of the triacylglycerides have been determined. The changes taking place in the composition and structure of these lipids on artificial infection of the plant by the fungal pathogenVerticillium dahliae have been elucidated.Institute of the Chemistry of Plant Substances, Uzbekistan Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 477–483, September–October, 1992.     

Phospholipids of ripeSophora japonica seeds

Nine classes of lipids have been isolated in homogenous form from ripe seeds of the Japanese pagoda tree, and their fatty acid compositions have been established. The positional distributions of the fatty acids in the phosphatidylcholines, phosphatidylethanolamines, and phosphatidylinositols have been determined.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 40 64 75. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 578–582, July–August, 1997.     

Lipids of the bark ofElaeagnus angustifolia branches

The compositions of the neutral lipids and the glyco- and phospholipids of the bark of branches ofElaeagnus angustifolia (fam. Elaeagnaceae) have been investigated. The fatty acid compositions of the acyl-containing classes have been established.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 40 64 75. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 41–44, January–February, 1998.     

Phospholipids of the pericarp ofCrataegus turkestanica

Phospholipids of the pericarp of Crataegus turkestanica have been studied and 12 classes of phospholipids have been detected, the main ones being phosphatidylcholine, phosphatidylethanolamine, and phosphatidylinositol. The composition and position distribution of the fatty acids of the main phospholipids have been studied.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 89 14 75. Translated from Khimiya Prirodnykyh Soedinenii,No. 4, pp. 562–563, July–August 1995. Original article submitted October 24, 1994.     

Use of the Vaskovsky-Svetashev reagent for complex detection of phospholipids and in their preparative separation by the TLC method

It has been established that the Vaskovsky-Svetashev reagent has no influence on the general and positional composition of the fatty acids of phospholipids. For the qualitative identification of PLs on one plate it is possible to use successively iodine, ninhdrin, the Dragendorff reagent, the Vaskovsky-Svetashev reagent, 50% H₂SO₄, and the Malachite Green reagent.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 40–42, January–February, 1988.     

Unsaponifiable substances of the lipids of the leaves ofHibiscus sp

The unsaponifiable substances of the lipids of the leaves ofHibiscus sp. have been studied. The compositions have been found of seven classes of compounds: hydrocarbons, carotenoids, tocopherols, isoprenols, triterpenols, and phytosterols and their esters.The materials of the paper were presented at the IInd International Symposium on the Chemistry of Natural Compounds (SCNC, Turkey, Eskiehir, October 22–24, 1996).Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 40 64 75. Translated from Khimiya Prirodnykh Soedinenii, Vol. 33, No. 1, pp. 49–51, January–February, 1997.     

Neutral lipids of the seeds ofOenothera biennis

The lipids and fatty acids of the seeds ofOenothera biennis L, fam. Onagraceae, have been studied with the aid of chemical, chromatographic, and spectral analyses. In the lipids we identified the usual and oxygenated acylglycerides and five classes of lipophilic substances. The structures of four epxy acids and of 22 hydroxy acids have been determined. The physiologically active 18:3(6,9,12)- acid was found in all the acylcontaining classes.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (371) 1206475. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 317–321, May–June, 1999.     

Phospholipids of the seeds ofFumaria vaillantii

The qualitative and quantitative compositions of the phospholipids from the seeds ofFumaria vaillantii L. have been determined. The total fatty acid compositions of all the classes of phospholipids and the position-distributions of the phosphatidylcholines, phosphatidylinositols, and phosphatidylethanolamines have been investigated.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 40 64 75. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 800–802, November–December, 1997.     

Study of the lignin of a cotton plant of the variety S-6524

The dioxane lignin (DLA) has been isolated from ripe stems of a cotton plant of the variety S-6524, and its empirical formula and molecular mass have been determined. This DLA differed from the DLA of a cotton plant of variety 108-F by a lower degree of hydroxylation and a higher degree of substitution of the aromatic rings.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, FAX (3712) 62 73 48. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 430–431, May–June, 1994.     

Epoxy and hydroxy acids of the seed oil ofGaleopsis bifida

The lipids of the seeds of the hemp nettle, which is toxic for ruminants have been studied. The composition of the fatty acids of five classes of lipids and the position-species composition of the triacylglycerols have been determined. The presence of epoxyacyl- and hydroxyacylglycerols in the oil and the structures of the acids of these lipids have been established. Squalene has been isolated from the oil.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 288–295, May–June, 1981.