Separation of radioactive histamine and some of its metabolites by one-dimensional paper chromatography

A simple one-dimensional paper chromatograph technique is described for the separation of radioactive histamine and some of its metabolites in blood and tissues. After removal of protein, the supernatant fluid (200 microliter) is subjected to two-stage one-dimensional paper chromatography. This enables large numbers of samples to be chromatographed simultaneously. Using this technique in conjunction with liquid scintillation counting, it was possible to monitor the small amounts (0.02 nCi) of radioactive histamine and its metabolites in serial blood samples.     

Separation of histamine and its metabolites using glass capillary gas chromatography and a nitrogen detector

Complete separation of the TMS derivatives of histamine, 1-methyl histamine and 3-methyl histamine was achieved in two minutes in a 7 m × 0.25 mm column coated with SP 2250. Using NPD, histamine was detectable at levels as low as 20 pg. This offers a rapid, sensitive procedure with potential for clinical diagnosis and food analysis.     

Separation assay of histamine and its metabolites in biological specimens

This review summarizes the determination methods for histamine and the metabolites in biological specimens by separation techniques, such as gas chromatography, liquid chromatography and capillary electrophoresis. The typical applications using these methods are also described in this review together with the characteristics of the methods. Copyright (c) 2008 John Wiley & Sons, Ltd.     

Studies on regioselective hydrogenation of thebaine and its conversion to hydrocodone

Thebaine was subjected to catalytic hydrogenation under a variety of conditions in order to determine the regioselectivity for C-6/C-7 versus C-8/C-14 olefin saturation.     

Asymmetric synthesis of fagomine and its congeners

The total synthesis of fagomine and its congeners 1–3 has been achieved starting from d-serine-derived Garner aldehyde 5 by catalytic ring-closing metathesis (RCM) for the construction of the piperidine ring followed by dihydroxylation.     

Separation of naphthalene-2,3-dicarboxaldehyde-derivatized-substance P and its metabolites by micellar electrokinetic chromatography

This paper describes the development of analytical methodology for the separation of naphthalene-2,3-dicarboxaldehyde (NDA)-derivatized substance P (CBI-SP) and five lysine-containing metabolites by micellar electrokinetic chromatography (MEKC). The effect of surfactant composition and organic modifiers on the separation was investigated. The final separation buffer consisted of 80 mM sodium cholate in 50 mM N-tris (hydroxymethyl)methyl-2-aminoethanesulfonic acid (TES), pH 7. All six lysine-containing peptides were separated under these conditions.     

Separation and detection of peroxynitrite and its metabolites by capillary electrophoresis with UV detection

A method for the separation and direct detection of peroxynitrite (ONOO(-)) and two of its degradation products, nitrite (NO(2)(-)) and nitrate (NO(3)(-)), using capillary electrophoresis with ultraviolet detection is described. The separation parameters were optimized and included electrokinetic injection, a run buffer consisting of 25 mM K(2)HPO(4) 7.5 mM DTAB, pH 12, and a field strength of -323 V/cm. A diode array UV detector was employed in these studies as it allowed the determination of all three species simultaneously. Nitrate and nitrite provided the maximum response at 214 nm while peroxynitrite generated the best response at 302 nm. All three species could be detected at 214 nm, while simultaneous detection at 214 and 302 nm positively identified each peak.     

Separation and identification of parathion and its metabolites in autopsy tissues by thin layer chromatography

Summary Thin-layer chromatography on magnesium oxide/starch with 4% ethanol in fusel oil will distinguish parathion and its metabolitep-nitrophenol from malathion, diazinon andm-systox in autopsy fluids and tissues. As little as 1g of parathion can be detected on the thin-layer plate with Rhodamine B.

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Zusammenfassung Dünnschiohtchromatographie auf Magnesiumoxid/ Stärkepulver mit Amylalkohol und 4% Ä hanol eignet sich zur Unterscheidung von Parathion und dessen Metaboliten p-Nitrophenol von Malathion, Diazinon und Metasystox in Körperflüssigkeiten und Geweben. 1g Parathion kann auf der Dünnschichtplatte noch mit Rhodamin B nachgewiesen werden.

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Paper read on 19. March 1969 at the Winter School of Forensic Science held at Nangal Dam.     

Derivatographic investigation of glass fibre adsorbents based on bentone clay

By means of thermal analysis the activation energy and the characteristics of glass fibre adsorbents upon dehydration were determined depending on the chemical composition, acidity and thermal treatment of bentone fibres.

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Identification and quantitative analysis of phendimetrazine and some of its metabolites in biological fluids

In urine, after oral doses of phendimetrazine to man, were found unchanged drug, the N-demethylated metabolite, phenmetrazine, and the N-oxide of phendimetrazine, but not N-hydroxyphenmetrazine; the metabolites were identified using t.l.c. and g.l.c. The stability of the drug and its metabolites in biological fluids and in ether was studied. A gas chromatographic procedure for the quantitative determination of unchanged drug, its N-oxide and phenmetrazine in urine, plasma and saliva was developed, the N-oxide being reduced before analysis.

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Identifizierung und quantitative Analyse von Phendimetrazin und einigen Metaboliten in biologischen Flüssigkeiten

Zusammenfassung Nach oralen Dosen von Phendimetrazin an Menschen wurde im Harn unverändertes Phendimetrazin, der N-demethylierte Metabolit, Phenmetrazin und das N-Oxid von Phendimetrazin, nicht jedoch N-Hydroxyphenmetrazin gefunden; die Metaboliten wurden mittels TLC und GLC identifiziert. Die Stabilität von Phendimetrazin und dessen Metaboliten wurde in biologischen Flüssigkeiten und in Ether untersucht. Es wurde eine gaschromatographische Methode für die quantitative Bestimmung von Phendimetrazin, seinem N-Oxid und Phenmetrazin in Harn, Plasma und Speichel entwickelt, wobei das N-Oxid vor der Analyse reduziert wurde.