共查询到20条相似文献,搜索用时 15 毫秒
1.
Dynamic light scattering is used as a sensitive probe of hybridization on DNA-functionalized colloidal gold nanoparticles. When a target DNA strand possesses an 8 base "dangling end", duplex formation on the surface of the nanoparticles leads to an increase in hydrodynamic radius. Duplex melting is manifested in a drop in hydrodynamic radius with increasing temperature, and the concentration dependence of the melting temperature provides a measure of the thermodynamics of binding. The hybridization thermodynamics are found to be significantly lower at higher hybridization densities than those previously reported for initial hybridization events. The pronounced deviation from Langmuir adsorption behavior is greater for longer duplexes, and it is, therefore, consistent with electrostatic repulsion between densely packed oligonucleotides. The results have implications for sensing and DNA-directed nanoparticle assembly. 相似文献
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According to the Dupré equation, the work of adhesion is equal to the surface energy difference in the separated versus the joined materials minus an interfacial energy term. However, if a liquid is at the interface between two solid materials, evaporation or condensation takes place under equilibrium conditions. The resulting matter exchange is accompanied by heat flow, and can reduce or increase the work of adhesion. Accounting for the energies requires an open-system control volume analysis based on the first law of thermodynamics. Depending on whether evaporation or condensation occurs during separation, a work term that is negative or positive must be added to the surface energy term to calculate the work of adhesion. We develop and apply this energy balance to several different interface geometries and compare the work of adhesion to the surface energy created. The model geometries include a sphere on a flat with limiting approximations and also with an exact solution, a circular disc, and a combination of these representing a rough interface. For the sphere on a flat, the work of adhesion is one half the surface energy created if equilibrium is maintained during the pull-off process. 相似文献
4.
Liu D Perdue RK Sun L Crooks RM 《Langmuir : the ACS journal of surfaces and colloids》2004,20(14):5905-5910
This paper describes immobilization of DNA onto the interior walls of poly(dimethylsiloxane) (PDMS) microsystems and its application to an enzyme-amplified electrochemical DNA assay. DNA immobilization was carried out by silanization of the PDMS surface with 3-mercaptopropyltrimethoxysilane to yield a thiol-terminated surface. 5'-acrylamide-modified DNA reacts with the pendant thiol groups to yield DNA-modified PDMS. Surface-immobilized DNA oligos serve as capture probes for target DNA. Biotin-labeled target DNA hybridizes to the PDMS-immobilized capture DNA, and subsequent introduction of alkaline phosphatase (AP) conjugated to streptavidin results in attachment of the enzyme to hybridized DNA. Electrochemical detection of DNA hybridization benefits from enzyme amplification. Specifically, AP converts electroinactive p-aminophenyl phosphate to electroactive p-aminophenol, which is detected using an indium tin oxide interdigitated array (IDA) electrode. The IDA electrode eliminates the need for a reference electrode and provides a steady-state current that is related to the concentration of hybridized DNA. At present, the limit of detection of the DNA target is 1 nM in a volume of 20 nL, which corresponds to 20 attomoles of DNA. 相似文献
5.
Lu M Hall JG Shortreed MR Wang L Berggren WT Stevens PW Kelso DM Lyamichev V Neri B Skinner JL Smith LM 《Journal of the American Chemical Society》2002,124(27):7924-7931
The structure-specific invasive cleavage reaction is a useful means for sensitive and specific detection of single nucleotide polymorphisms, or SNPs, directly from genomic DNA without a need for prior target amplification. A new approach integrating this invasive cleavage assay and surface DNA array technology has been developed for potentially large-scale SNP scoring in a parallel format. Two surface invasive cleavage reaction strategies were designed and implemented for a model SNP system in codon 158 of the human ApoE gene. The upstream oligonucleotide, which is required for the invasive cleavage reaction, is either co-immobilized on the surface along with the probe oligonucleotide or alternatively added in solution. The ability of this approach to unambiguously discriminate a single base difference was demonstrated using PCR-amplified human genomic DNA. A theoretical model relating the surface fluorescence intensity to the progress of the invasive cleavage reaction was developed and agreed well with experimental results. 相似文献
6.
Recently, it has been found that individual giant DNA molecules exhibit a discrete transition, or first order phase‐transition, between the compact folded state and the elongated coiled state, i.e., the folding transition. In order to clarify the thermodynamics in the folding transition of single DNA molecules, we have studied the temperature effect on the bimodal distribution of conformation for the ensemble of T4DNA chains (166 kbps) in both poly(ethylene glycol) (PEG) and spermidine (SPD), using single‐chain observation with fluorescence microscopy. From the van't Hoff relationship, the entropy change in the transition from the compact state to the unfolded state is deduced as, ΔS = +11, +38 k/molecule in the aqueous solution of PEG with sodium chloride and potassium chloride, respectively, where k is Boltzmann's constant, whereas, ΔS with SPD is estimated to be −32 k/molecule. The values of ΔS with the transition are discussed in term of the translational entropy of counterions together with the hydration effect. 相似文献
7.
Jordi Porta 《International journal of quantum chemistry》1978,13(4):499-507
The thermodynamics of the DNA helix-coil transition is studied, starting from the thermodynamical potential difference between the states helix and coil; this potential difference is understood as the difference in free energies. With only three parameters obtained from experimental data different quantities of the T2 phage DNA molecule are calculated. It is observed that the phase transition is of second order. 相似文献
8.
Up to now, the development of the electrochemical DNA hybridization sensors relied on solid electrodes, on which both the hybridization and detection steps have been performed. Here we propose a new method in which the DNA hybridization is performed at commercially available magnetic beads and electrochemical detection on detection electrodes (DE). Due to minimum nonspecific DNA adsorption at the magnetic beads, very high specificity of the DNA hybridization is achieved. Optimum DE can be chosen only with respect to the given electrode process. It is shown that high sensitivity and specificity in the detection of relatively long target DNAs can be obtained (a) by using cathodic stripping voltammetry at mercury or solid mercury amalgam DEs for the determination of purine bases, released from DNA by acid treatment, and (b) by enzyme-linked immunoassay of target DNA modified by osmium tetroxide,2,2'-bipyridine (Os,bipy) at carbon DEs. Direct determination of Os,bipy at mercury and carbon electrodes is also possible. 相似文献
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Zoli M 《The Journal of chemical physics》2011,135(11):115101
The imaginary time path integral formalism is applied to a nonlinear Hamiltonian for a short fragment of heterogeneous DNA with a stabilizing solvent interaction term. Torsional effects are modeled by a twist angle between neighboring base pairs stacked along the molecule backbone. The base pair displacements are described by an ensemble of temperature dependent paths thus incorporating those fluctuational effects which shape the multisteps thermal denaturation. By summing over ~10(7)-10(8) base pair paths, a large number of double helix configurations is taken into account consistently with the physical requirements of the model potential. The partition function is computed as a function of the twist. It is found that the equilibrium twist angle, peculiar of B-DNA at room temperature, yields the stablest helicoidal geometry against thermal disruption of the base pair hydrogen bonds. This result is corroborated by the computation of thermodynamical properties such as fractions of open base pairs and specific heat. 相似文献
11.
自从1963-1964年发现线粒体DNA(mtDN)以来,人们对线粒体DNA的结构、功能等方面进行了很多研究.高等植物线粒体DNA的研究也有不少报导.线粒体DNA之所以引起人们广泛兴趣决非偶然.线粒体具有独立复制的能力,它有自己独特的DNA、rRNA、tRNA、核糖体.它是细胞的动力站,生物氧化链上某些重要的酶的一部分亚基是由线粒体基因组编码的.对线粒体DNA的深入研究,肯定会对线粒体起源问题提供有价值的线索.此外,植物的雄性不育、真核细胞的抗药性等,也都可能与线粒体DNA有关.尽管如此,线粒体及线粒体DNA的某些功能至今仍… 相似文献
12.
Genomagnetic electrochemical assays of DNA hybridization 总被引:1,自引:0,他引:1
An electrochemical genomagnetic hybridization assay has been developed to take advantage of a new and efficient magnetic separation/mixing process, the amplification feature of enzyme labels, and single-use thick-film carbon transducers operated in the pulse-voltammetric mode. It represents the first example of coupling a magnetic isolation with electrochemical detection of DNA hybridization. The new protocol employs an enzyme-linked sandwich solution hybridization, with a magnetic-particle labeled probe hybridizing to a biotinylated DNA target that captures a streptavidin-alkaline phosphatase (AP). The alpha-naphthol product of the enzymatic reaction is quantitated through its well-defined, low-potential (+0.1 V vs. Ag/AgCl) differential pulse-voltammetric peak at the disposable screen-printed electrode. The efficient magnetic isolation is particularly attractive for electrical detection of DNA hybridization which is commonly affected by the presence of non-hybridized nucleic acid adsorbates. The new biomagnetic processing combines such magnetic separation with a low-volume magnetic mixing, and allows simultaneous handling of 12 samples. The attractive bioanalytical behavior of the new enzyme-linked genomagnetic electrical assay is illustrated for the detection of DNA segments related to the breast-cancer BRCA1 gene. 相似文献
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《Electrochemistry communications》2003,5(10):887-891
A label-free electrochemical detection protocol for DNA hybridization is reported for the first time by using a gold electrode (AuE). The oxidation signal of guanine was monitored at +0.73 V by using square wave voltammetry (SWV) on self-assembled l-cysteine monolayer (SAM) modified AuE. The electrochemical determination of hybridization between an inosine substituted capture probe and native target DNA was also accomplished. 6-mer adenine probe was covalently attached to SAM via its amino link at 5′ end. Then, 6-mer thymine-tag of the capture probe was hybridized with the adenine probe, thus left the rest of the oligonucleotide available for hybridization with the target. The dependence of the guanine signal upon the concentration of the target was observed. Probe modified AuE was also challenged with non-complementary and mismatch containing oligonucletides. Label-free detection of hybridization on AuE is greatly advantageous over the existing carbon and mercury electrode materials, because of its potential applicability to microfabrication techniques. Performance characteristics of the genosensor are described, along with future prospects. 相似文献
14.
The increasing demand for simple, low-cost, rapid, sensitive and label-free methods for the detection of DNA sequences and the presence of single nucleotide polymorphisms (SNPs) has become an important issue in biomedical research. In this work, we studied the performances of several chemically modified graphene nanomaterials as sensing platforms by using the electrochemical impedance spectroscopy technique for the detection. We employed a hairpin DNA as a highly selective probe for the detection of SNP correlated to Alzheimer's disease. We believe that our findings may present a foundation for further research and development in graphene-based impedimetric biosensing. 相似文献
15.
Thomas Schüler Alla Nykytenko Andrea Csaki Robert Möller Wolfgang Fritzsche Jürgen Popp 《Analytical and bioanalytical chemistry》2009,395(4):1097-1105
The performance of DNA microarrays strongly depends on their surface properties. Furthermore, the immobilization method of
the capture molecules is of importance for the efficiency of the microarray in terms of sensitivity and specificity. This
work describes the immobilization of single-stranded capture oligonucleotides by UV cross-linking on silanated (amino and
epoxy) glass surfaces. Thereby we used amino (NH2) and poly thymine/poly cytosine modifications of the capture sequences as well as unmodified capture molecules. The results
were compared to UV cross-linking of the same DNA oligonucleotides on unmodified glass surfaces. Immobilization and hybridization
efficiency was demonstrated by fluorescence and enzyme-induced deposition of silver nanoparticles. We found out that single-stranded
DNA molecules do not require a special modification to immobilize them by UV cross-linking on epoxy- or amino-modified glass
surfaces. However, higher binding rates can be achieved when using amino-modified oligonucleotides on an epoxy surface. The
limit of detection for the used settings was 5 pM. 相似文献
16.
Carbon nanotube-enhanced electrochemical DNA biosensor for DNA hybridization detection 总被引:5,自引:0,他引:5
A novel and sensitive electrochemical DNA biosensor based on multi-walled carbon nanotubes functionalized with a carboxylic acid group (MWNTs-COOH) for covalent DNA immobilization and enhanced hybridization detection is described. The MWNTs-COOH-modified glassy carbon electrode (GCE) was fabricated and oligonucleotides with the 5'-amino group were covalently bonded to the carboxyl group of carbon nanotubes. The hybridization reaction on the electrode was monitored by differential pulse voltammetry (DPV) analysis using an electroactive intercalator daunomycin as an indicator. Compared with previous DNA sensors with oligonucleotides directly incorporated on carbon electrodes, this carbon nanotube-based assay with its large surface area and good charge-transport characteristics dramatically increased DNA attachment quantity and complementary DNA detection sensitivity. This is the first application of carbon nanotubes to the fabrication of an electrochemical DNA biosensor with a favorable performance for the rapid detection of specific hybridization. 相似文献
17.
Ge D Wang X Williams K Levicky R 《Langmuir : the ACS journal of surfaces and colloids》2012,28(22):8446-8455
Monolayer films of nucleic acids on solid supports are encountered in a range of diagnostic and bioanalytical applications. These applications often rely on elevated temperatures to improve performance; moreover, studies at elevated temperatures can provide fundamental information on layer organization and functionality. To support such applications, this study compares thermostability of oligonucleotide monolayers immobilized to gold by first coating the gold with a nanometer-thick film (an "anchor layer") of a polymercaptosiloxane, to which DNA oligonucleotides are subsequently tethered through maleimide-thiol conjugation, with thermostability of monolayers formed via widely used attachment through a terminal thiol moiety on the DNA. The temperature range covered is from 25 to 90 °C. After confirming stability of immobilization and, more importantly, retention of hybridization activity even under the harshest conditions investigated, these thermostable films are used to demonstrate measurements of (1) reversible surface melting transitions and (2) temperature dependence of competitive hybridization, when fully matched and mismatched sequences compete for binding to immobilized DNA oligonucleotides. The competitive hybridization experiments reveal a pronounced impact of temperature on rates of approach to equilibrium, with kinetic freezing into nonequilibrium states close to room temperature and rapid approach to equilibrium at elevated temperatures. Modeling of competitive surface hybridization equilibria using thermodynamic parameters derived from surface melting transitions of the individual sequences is also discussed. 相似文献
18.
Flechsig GU Peter J Hartwich G Wang J Gründler P 《Langmuir : the ACS journal of surfaces and colloids》2005,21(17):7848-7853
The detection of DNA hybridization is of central importance to the diagnosis and treatment of genetic diseases. Due to cost limitations, small and easy-to-handle testing devices are required. Electrochemical detection is a promising alternative to evaluation of chip data with optical readout. Independent of the actual readout principle, the hybridization process still takes a lot of time, hampering daily use of these techniques, especially in hospitals or doctor's surgery. Here we describe how direct local electrical heating of a DNA-probe-modified gold electrode affects the surface hybridization process dramatically. We obtained a 140-fold increase of alternating current voltammetric signals for 20-base ferrocene-labeled target strands when elevating the electrode temperature during hybridization from 3 to 48 degrees C while leaving the bulk electrolyte at 3 degrees C. At optimum conditions, a target concentration of 500 pmol/L could be detected. Electrothermal regeneration of the immobilized DNA-probe strands allowed repetitive use of the same probe-modified electrode. The surface coverage of DNA probes, monitored by chronocoulometry of hexaammineruthenium(III), was almost constant upon heating to 70 degrees C. However, the hybridization ability of the probe self-assembled monolayer declined irreversibly when using a 70 degrees C hybridization temperature. Coupling of heated electrodes and highly sensitive electrochemical DNA hybridization detection methods should enhance detection limits of the latter significantly. 相似文献
19.
M. M. Ulyashova M. Yu. Rubtsova T. Bachmann A. M. Egorov 《Moscow University Chemistry Bulletin》2008,63(2):75-79
A method of hybridization analysis on a DNA microarray using colorimetric detection on the basis of horseradish peroxidase has been developed. The effectiveness of the incorporation of biotin as a label in the DNA molecule in the PCR process is estimated and the conditions of hybridization of the biotin-labeled DNA with oligonucleotides immobilized on the surface of the array are optimized. The possibility of using the developed method is shown by the example of genotyping of CTX-M β-lactamases. 相似文献
20.
Thompson LA Kowalik J Josowicz M Janata J 《Journal of the American Chemical Society》2003,125(2):324-325
A new approach to the realization of the electrochemical DNA hybridization probe is described. It is based on the exchange of chloride ion between the polypyrrole layer and the buffer. The shape of the cyclic voltammogram is modulated by the negative charge density at this interface, resulting from the immobilized target DNA. The negative charge density increases when the complementary DNA hybridizes with the probe DNA. The hybridization event can be clearly seen in the cyclic voltammogram before and after the addition of the probe DNA. The immobilization is accomplished via the Mg2+ bridging complex between phosphonic acid groups of the poly[2,5-dithienyl-(N-3-phosphorylpropyl)pyrrole] grafted at the polypyrrole surface and the phosphate groups of the target DNA. 相似文献