一种基于喹唑啉酮衍生物的荧光探针对次氯酸根离子的识别

次氯酸根(ClO~-)在人体免疫系统中发挥着重要的作用,其识别与检测备受关注。本文设计合成了一种含有喹唑啉酮骨架的腙型荧光探针(HEMQ),并通过~1H NMR、~(13)C NMR、高分辨质谱(HRMS)表征了其结构。探针HEMQ在V(乙醇)∶V(水)=1∶1(c(PBS)=0.02 mol/L,pH=8.7)溶液中对ClO~-具有良好的选择性且响应快速,荧光发生显著猝灭。探针HEMQ对ClO~-具有较高的灵敏度,检测限为1.0×10-4mol/L。此外,ClO~-可引起探针溶液由黄色到无色的颜色变化,因此HEMQ可作为比色、荧光双通道响应的ClO~-探针。     

咔唑-二氨基马来腈类荧光探针的合成及对Cu2+和ClO-的检测

利用咔唑醛与二氨基马来腈合成了一种对Cu²⁺和ClO^-的响应的荧光探针分子CMN。探针分子与Cu²⁺作用后,紫外吸收光谱在394 nm处的吸光度迅速下降,颜色由黄色褪至无色;荧光光谱在452 nm处的发射峰迅速增强,出现明显的蓝色荧光。探针与ClO^-作用后,溶液发生褪色,并在375 nm和394 nm出现强荧光发射,发出淡蓝色荧光。CMN通过配位过程实现了对Cu²⁺的检测,其检测限为47μmol/L;CMN在ClO^-催化下亚胺键水解得到咔唑醛,实现了对ClO^-的检测,检测限3.06μmol/L。CMN可作为荧光检测试纸快速检测环境中的Cu²⁺和ClO^-。     

基于菲并咪唑的ON⁃OFF⁃ON双比色荧光探针及细胞成像

设计合成了对称型菲并咪唑荧光探针PIP-ph-PIP, 并对其结构进行了表征和确认. 随着探针溶液中水体积分数的增加, 探针的聚集程度逐渐增加, 荧光强度先增强后猝灭. 荧光光谱测试结果表明, 在水相体系中探针PIP-ph-PIP能以ON-OFF-ON的方式分别对Ag⁺和SCN^-, Cu²⁺和PO³₄ ^-进行连续识别, 且连续识别效果可通过裸眼比色观察, 其中对Ag⁺识别具有超低检出限(6.1 nmol/L). 结果表明, 探针PIP-ph-PIP可应用于活体HeLa细胞中Ag⁺和Cu²⁺的定性分析及实际水样中Ag⁺和Cu²⁺的定量检测.     

可视化检测次氯酸的苯并咪唑类 荧光增强型探针

合成了一种新型的苯并咪唑类荧光增强型探针2-{[4-(1H-苯并咪唑-2-基)苯亚甲基]氨基}-3-氨基马来腈(ZY12), 用光谱方法研究了其对次氯酸的识别作用. 实验结果表明, ZY12对ClO ^-表现出较高的选择性和灵敏度, 且响应快速, 抗干扰能力强; 将ClO ^-加入到ZY12溶液中后, 反应体系的荧光强度随ClO ^-浓度的增加而逐渐增强, 并伴随着荧光颜色由微蓝色到亮蓝色的变化, 可实现对ClO ^-的可视化检测; 在1.8×10 ^-6~3.4×10 ^-5 mol/L浓度范围内, ZY12能够定量检测ClO ^-, 检测限为2.8×10 ^-7 mol/L; ZY12可应用于不同水样中ClO ^-的检测, 并能对HeLa活细胞中的ClO ^-进行荧光成像.     

A pinacol boronate caged NIAD-4 derivative as a near-infrared fluorescent probe for fast and selective detection of hypochlorous acid

A pinacol boronate caged NIAD-4 derivative was demonstrated to be a near-infrared fluorescent probe for fast and selective detection of hypochlorite over other ROS species.     

线粒体靶向的近红外HClO/ClO-荧光探针的研究进展

HClO/ClO-作为细胞质中一种重要的活性氧(ROS),源自线粒体,参与各种生理和病理过程,因此快速有效检测HClO/ClO-具有重要的生物学及生理学意义.荧光分析法因其灵敏度高、响应时间快、选择性高、成本低和操作简便等优点而备受关注.更重要的是,使用荧光探针可以在体外和体内可视化检测.近年来,为了研究HClO/Cl...     

Azomethine-H as a Highly Selective Fluorescent Probe for Fe3+ Detection in 100％ Aqueous Solution and Its Application in Living Cell Imaging

A simple assay for the detection of Fe³⁺ in water by means of fluorescence spectroscopy was developed based on a commercially available reagent, Azomethine-H(A-H), allowing sensing trace levels of Fe³⁺ with high selectivity over other cations. A significant fluorescence quenching of A-H at 424 nm was found after its binding with Fe³⁺ in 100% aqueous solution at pH=7.0, while other physiologically relevant metal ions posed little interference. The fluorescence responses can be well described by the modified Stern-Volmer equation. A good linear relationship(R²=0.9904) was observed up to 1.6×10^-5 mol/L Fe³⁺ ions. The detection limit, calculated via the 3σ IUPAC(international union of pure and applied chemistry) criteria, was 1.95×10^-7 mol/L. Moreover, the colorimetric and fluorescent response of A-H to Fe³⁺ can be conveniently detected by the naked eye, providing a facile method for visual detection of Fe³⁺. The proposed method was used to determine Fe³⁺ in water samples. Moreover, inverted fluorescence microscopy imaging using human umbilical vein endothelial cells shows that A-H can be used as an effective fluorescent probe for detecting Fe³⁺ in living cells.     

Cr3+比色荧光探针的合成及细胞成像应用

以2,3,3-三甲基-3H-苯并[e]吲哚和对二甲氨基苯甲醛为原料, 乙醇作溶剂, 在酸性催化条件下, 通过一步反应合成了比色荧光探针B. 在EtOH/HEPES(pH=7.4)体积比为9∶1的混合体系中, 向探针B溶液中加入Cr ³⁺后, 溶液颜色由淡黄色变为紫红色, 说明探针B可以对Cr ³⁺进行裸眼识别. 紫外-可见吸收光谱和荧光发射光谱分析表明, 探针B对Cr ³⁺的选择性好、 灵敏度高且对EDTA有良好的接力识别. 探针B对Cr ³⁺的结合常数K_a=0.28×10 ² mol/L, 检出限为1.90×10 ^-8 mol/L, 该检出限低于世界卫生组织(WHO)规定的饮用水中Cr ³⁺的最大含量(9.60×10 ^-8 mol/L). 利用荧光发射光谱对实际水样中Cr ³⁺的浓度进行了定量检测. 探针B也可应用于对活细胞中Cr ³⁺的检测, 具有较好的应用前景和实用价值.     

基于香豆素的比率型次氯酸荧光探针

次氯酸(HCl O)是生物体内重要的活性氧(ROS)之一,在人类免疫功能系统中扮演着重要的角色,有助于对入侵细菌和病原体进行破坏。本文设计并合成了基于香豆素为母体单元的比率型次氯酸荧光探针。研究结果表明,该探针对次氯酸识别显示出较高的选择性,检测线低至12 mol/L,荧光响应可在5 s内迅速完成,并伴随着溶液颜色由无色转变为黄绿色。其它常见的阴离子及氧化型物质对次氯酸检测均无干扰。此外,高分辨率质谱、荧光光谱和紫外可见光谱变化共同证实了该探针对次氯酸的检测机制为次氯酸对探针氧化水解。     

基于咔唑的双光子荧光次氯酸根探针光学性质及响应机理

采用含时密度泛函理论与响应函数理论相结合的方法,研究了两种实验室新合成的水溶性双光子荧光次氯酸根（ClO^-）探针分子HCH和HCM的单光子吸收、双光子吸收和荧光发射性质。计算结果表明,HCH和HCM分子与ClO^-反应后,生成物的光吸收和光发射性质均发生明显变化,相应的吸收和发射峰位都发生了明显蓝移,荧光强度展现出明显的增强。另外,两探针分子都具有较大的双光子吸收截面,且与ClO^-反应后,生成物的双光子吸收截面值显著增加,因此两分子均可作为性能优良的双光子荧光探针分子。此外,通过分析HCH和HCM分子与ClO^-反应前后的Mulliken电荷布居情况,从理论上证实了该系列荧光探针分子的识别机理是C＝N异构化。     

以苯并咪唑萘酰亚胺为荧光团高选择快速检测H2S的荧光探针

以1,8-萘二甲酸酐为起始原料合成一种萘酰亚胺衍生物3-叠氮基-7H-苯并[de]苯并[4,5]咪唑[2,1-a]异喹啉(DBNG),利用傅里叶变换红外光谱(FT-IR)和核磁共振波谱(NMR)等测试手段确定其结构,并将其作为小分子荧光探针用于检测H2S,系统研究了其荧光特性,结合理论计算,探究其可能的检测机制.结果表...     

A ratiometric near-infrared naphthalimide-based fluorescent probe with high sensitivity for detecting Fe2+ in vivo

Iron is one of the essential trace elements in the human body. It plays an important role in human biology and pathology. Deregulation of iron levels in cells is associated with disease development. In this work, we synthesized a novel near-infrared intramolecular charge transfer (ICT) based ratiometric fluorescent probe to detect Fe²⁺, by using naphthalimide and indole moieties as building blocks. Our work showed that the radiometric probe has excellent selectivity, sensitivity and rapid response. Moreover, we could successfully perform real-time monitoring of Fe²⁺ in HeLa cells and C. elegans.     

A mitochondria-targeted fluorescent probe for ratiometric detection of hypochlorite in living cells

A new fl uorescent probe 1 was designed for mitochondrial localization and ratiometric detection of hypochlorite in living cells. It is noteworthy that a high Pearson’s co-localization coeffi cient (Rr) we have obtained was calculated to be 0.97.     

基于牛血清白蛋白功能化金纳米棒的荧光探针测定Hg2+

建立了一种以牛血清白蛋白功能化的金纳米棒(BSA-Cys-GNRs)为荧光探针检测Hg²⁺的新方法。以半胱氨酸作为连接臂成功将牛血清白蛋白修饰在金纳米棒表面,通过紫外可见吸收光谱、荧光光谱、红外光谱和荧光倒置显微镜等多种分析方法对材料进行表征。研究发现,在295nm波长光激发下,BSA-Cys-GNRs探针在338nm显示强荧光,而Hg²⁺能够有效地猝灭BSA-Cys-GNRs的荧光。对一系列影响猝灭效果的因素进行考察,得出pH 4.0、孵育时间5.0min为最佳检测条件。Mn²⁺、K⁺、Ni²⁺、Na⁺、Cr³⁺、Cd²⁺、Mg²⁺、Cu²⁺、Ca²⁺、Al³⁺和Zn²⁺对BSA-Cys-GNRs的荧光信号没有明显的影响。当Hg²⁺的浓度为0.04444~8.888μmol·L^-1时,荧光猝灭效率与Hg²⁺的浓度之间存在良好的线性关系,检测限为8.08nmol·L^-1。将该方法用于环境水样中Hg²⁺的检测,回收率为98.9%~105.0%。     

A carbazole-hemicyanine dye based ratiometric fluorescent probe for selective detection of bisulfite (HSO3−) in cells and C. elegans

A colorimetric and ratiometric fluorescent probe for selective detection of HSO₃^- based on the 1,4-nucleophilic addition reaction was successfully synthesized and applied to the detection of exogenous HSO₃^- in Hela cells and C. elegans.     

A new fluorescent probe of rhodamine B derivative for the detection of copper ion

A new fluorescent probe, rhodamine B hydrazide oxalamide (RBHO), which shows very weak fluorescence, was synthesized, and its fluorescence could be substantially enhanced by the addition of copper ion. The probe shows a high selectivity and sensitivity to copper ion by forming a 1:1 complex in acetonitrile, and the chelating is reversible. Limit of detection for copper ion in acetonitrile was found to be 3.7 × 10⁻⁸ mol L⁻¹. It was also found that copper ion could catalyze the hydrolysis of the probe in 50% (v/v) buffered (10 mM Tris–HCl, pH 7.0) water/acetonitrile giving a highly fluorescent product, and the fluorescence detection of copper ion was developed in this neutral buffered media with a detection limit of 6.4 × 10⁻⁷ mol L⁻¹. Determination of copper ion in water and synthetic samples in the presence of different interfering metal ions was successfully carried out with the new probe RBHO.     

基于香豆素343的荧光探针快速检测亚硫酸根

以香豆素343和2-苯并噻唑乙腈为原料,合成了香豆素343-亚硫酸根离子探针(Coumarin 343-SO₂),并用¹H NMR、¹³C NMR、MS和HRMS等技术手段对合成的化合物进行了表征。 该探针与亚硫酸根离子(SO₃^2-)发生亲核加成反应后,阻断了苯并噻唑与香豆素的共轭结构,从而引起荧光强度的变化,达到检测亚硫酸根离子的目的。 此探针对SO₃^2-具有响应快、高灵敏度、高选择性及检测限低至0.08 μmol/L的特点,其它常见的阴离子及还原性物质对SO₃^2-的检测均无干扰。 此外,该探针具有良好的细胞膜通透性,可用于活细胞中对SO₃^2-进行荧光成像。     

A Highly Selective and Sensitive Ratiometric Fluorescent Probe for Hypochlorite and Its Application

A novel water-soluble hybrid fluorescein-coumarin dye(FLNC) fluorescence ratiometric probe to recognize OCl^- was designed and synthesized. The most prominent feature of the probe is that the molecular structure contains two different fluorophores, and the fluorescein moiety was connected to coumarin moiety by diacylhydrazine linker. When OCl^- was added, the two fluorophores in the FLNC probe could have a rapid respond simultaneously, with high selectivity and sensitivity towards OCl^- among other reactive oxygen species/reactive nitrogen species(ROS/RNS) and anions, and the detection limit was measured to be 0.30 μmol/L. In addition, the color changes of the solution could be observed by the naked eye and have a short response time(completed within 1 min), which suggestes that the probe FLNC has the potential to be used for real-time detection.     

一种香豆素类锌离子荧光探针的合成及性能

通过Pechmann法使间苯二酚和乙酰乙酸乙酯发生脱水缩合反应,合成了4-甲基-7-羟基香豆素,再与六次甲基四胺反应,生成7-羟基-4-甲基-8-甲酰基香豆素,最终与吲哚酰肼发生席夫碱反应合成一种香豆素类荧光探针L。通过核磁共振波谱仪(NMR)、质谱(MS)、傅里叶变换红外光谱仪(FTIR)、荧光发射光谱仪和紫外可见分光光度计等技术手段研究该探针L的结构及其荧光性能。结果表明,探针L与Zn~(2+)的配合比为1∶1,对Zn~(2+)的检测限达到3.6×10~(-8)mol/L,降低了对Zn~(2+)的检测浓度,同时探针L对锌离子具有高选择性,可应用于在生物体内Zn~(2+)的检测。     

A ratiometric fluorescent probe for palladium detection based on an allyl carbonate group functionalized hemicyanine dye

A novel ratiometric fluorescent probe for palladium species was synthesized based on an allyl carbonate group, a novel reaction site, and a hemicyanine dye. The probe displays relatively rapid response, high selectivity, and anti-disturbance toward palladium in HEPES buffers without additional reagents. The detection mechanism, palladium triggers the cleavage of allyl carbonate in the probe and then decarboxylation of the product to induce the ratiometric fluorescence response, was verified using UV–vis and mass spectrometry analysis. The probe was successfully applied for ratiometric fluorescent detection of palladium in tap water, river water, and in fetal bovine serum.