Triton X-100对血红蛋白/利巴韦林/H2O体系中血红蛋白性质和药物释放的影响

通过紫外光谱、荧光光谱、zeta电位、电导率、高效液相色谱和影像分析等方法研究了Triton X-100对血红蛋白/利巴韦林/H2O体系中血红蛋白性质和药物控制释放的影响. 研究结果表明, 随着Triton X-100浓度的增大, 荧光强度、荧光偏振、zeta电位和蛋白的形貌均发生较大的变化, Triton X-100能使增溶定位在蛋白表面中的利巴韦林逐渐游离出来. 当Triton X-100浓度大于1×10^-5 mol·L-1 时, Triton X-100 与血红蛋白的作用占主导地位, 血红蛋白开始明显变性. 少量Triton X-100能保护蛋白免受药物的影响.     

Influences of Triton X-100 on Hemoglobin Behaviors in Hemoglobin/Acyclovir/Triton X-100/H2O System

The influences of Triton X-100 on hemoglobin （Hb） behaviors were studied by the methods of UV-Vis spectrum, fluorescence spectrum, HPLC, conductivity, zeta potential and negative-staining transmission electron microscope in Hb/acyclovir/Triton X-100/H2O system. With the increase of Triton X-100 concentration in the system, the percentage of the free acyclovir increased from 58%--63% to 90%--94%. The static quenching constant and the association number of acyclovir to Hb decreased. The fluorescence spectrum, conductivity, zeta potential, fluorescence polarization and negative-staining morphology of Hb tended to recover to those of the original state of Hb in the same concentration of Hb. The interaction between Triton X-100 and Hb is stronger than that between acyclovir and Hb. Most Triton-X-100 was associated with Hb at low Triton X-100 concentration. But the interaction of Triton X-100 with Hb was apparently dominant in high Triton X-100 concentration. The Hb structure was unfolded and finally denatured.     

Lubrication of the mixed system of Triton X-100/n-C10H21OH/H2O lamellar liquid crystal and ZnS nanoparticles

ZnS nanoparticles could be synthesized, when two kinds of Triton X-100/n-C₁₀H₂₁OH/H₂O lamellar liquid crystal were mixed, in which Zn(CH₃COO)₂ and Na₂S were dissolved in the solvent layer, respectively. The size of ZnS nanoparticles was about 10 nm and limited by the thickness of the solvent layer of the lamellar liquid crystal. The lubrication properties of the mixed system of Triton X-100/n-C₁₀H₂₁OH/H₂O lamellar liquid crystal and ZnS nanoparticles were determined. The results showed that the presence of ZnS nanoparticles could improve the anti-wear ability of the Triton X-100/n-C₁₀H₂₁OH/H₂O lamellar liquid crystal and decrease its friction coefficient.     

AOT/Triton X-100混合反胶束体系中假丝酵母脂肪酶催化蓖麻油水解的活性

研究了二(2-乙基己基)琥珀酸磺酸钠(AOT)/Triton X-100混合反胶束体系中假丝酵母脂肪酶(candida rugosa lipase)催化蓖麻油水解的反应. 考察了Triton X-100占总表面活性剂的摩尔分数(x(Triton X-100))、水与总体表面活性剂的摩尔比(ω0)、pH值、反应温度以及底物蓖麻油的浓度等因素对酶活性的影响. 研究结果表明, 加入非离子表面活性剂Triton X-100可以使假丝酵母脂肪酶的活性得到显著提高, 但是当底物蓖麻油的浓度大于0.24 mol·L-1时, 会对假丝酵母脂肪酶产生抑制作用.     

Semidifferential Electroanalysis of Aqueous Triton X—100／Toluene／H2O Microemulsion

Semidifferential electroanalysis is used as an effective method for investigating microstructure and structural transition of Triton X-100 microemulsions.     

TritonX-100-5-Br-PADAP光度法测定发样中铁

在表面活性剂Triton X-100存在下,以5－Br-PADAP作显色剂,光度法测定发样中铁,结果在所选浓度范围内线性关系良好,稳定时间长,回收率平均为98．14％。     

Dynamic surface tensions of micellar Triton X-100 solutions

Considering surfactant solutions at concentrations exceeding the CMC, another relaxation process besides diffusion occurs, also affecting the dynamic surface tension. The latter equilibration process concerns a micellisation/demicellisation process, representing the disintegration of micelles into monomers. The micellisation kinetics are accounted for by adding a single source term to the diffusion equation of the free monomers.

In the present paper the integration of the diffusion equation is avoided by using the concept of the diffusion penetration depth. Nevertheless, when this approximation is made, good agreement is achieved between experiment and theory for micellar Triton X-100 solutions. Moreover, it follows that diffusion of micelles may not be neglected.     

Influences of Triton X-100 micelle on the microstructure of HSA

The effects of Triton X-100 molecule and micelle on the microstructure and properties of HSA are studied by the some methods of UV spectrum, fluorescence spectrum, fluorescence polarization, circular dichroism, conductivity, and zeta potential. With the increase of Triton X-100 concentration, the UV absorbance, fluorescence intensity of HSA, and the system conductivity all first decrease and then increase. The zeta potential of HSA first goes up and then down. The percents of the β-sheet, random, turn structures, and the polarization of HSA increase but the percent of the α-Helix of HSA decreases. When Triton X-100 concentration is more than 1.0×10⁻³ mol l⁻¹, the structure parameters change obviously especially for the percents of random and turn structures.     

Triton X-100在乙二醇中的胶体性质

表面活性剂在非水溶剂中聚集态的研究所见的报导与水体系相比要少得多．然而近年来对非水体系的研究日益增多．这是因为表面活性剂在非水溶剂中的应用日趋增加，但与水体系相反，对非水体系的性质了解甚少．对表面活性剂非水体系的研究还有助于对其聚集体的共溶剂效应有更深的认识．TritonX－100（以下简写为TX－100）是典型的非离子表面活性剂，在工业上有着广泛的应用．乙二醇在多醇中与水的化学结构最相近问，然而它却是水结构的破坏剂，加入少量的乙二醇会使水的冰点急剧下降．乙二醇又是蛋白质的变性溶剂．表面活性剂在乙二醇中的胶…     

Triton X-100六角液晶相中制备镁铝层状双金属氢氧化物纳米片及其药物载体应用

以Triton X-100 六角相溶致液晶作微反应器, 采用共沉淀法制备了镁铝层状双金属氢氧化物(LDHs)纳米薄片(L-LDHs). 以双氯芬酸钠(DS)为药物模型分子, 采用离子交换法制备了DS插层LDHs (DS/L-LDHs)纳米杂化物, 在37.0 ℃、pH=7.2的缓冲溶液中, 考察了纳米杂化物的药物释放性能, 并与传统溶液共沉淀法制备的镁铝LDHs (S-LDHs)纳米片状颗粒进行了对比. 采用粉末X射线衍射(XRD)、傅里叶变换红外(FT-IR)光谱、场发射扫描电镜(FE-SEM)、透射电镜(TEM)和N₂吸附-脱附等技术对所制备的LDHs和DS/LDHs 样品的晶体结构、比表面积、形貌特征等进行了表征. 结果表明, L-LDHs比S-LDHs具有更低的片厚度, 更高的比表面积和药物负载量, 所形成的DS/L-LDHs纳米杂化物药物释放速率也明显低于DS/S-LSHs, 即L-LDHs更适于作药物载体. DS/L-LDHs纳米杂化物的药物释放过程符合准二级动力学方程, 受颗粒内部扩散过程控制. 溶致液晶模板法可实现LDHs的形貌可控制备, 为LDHs基功能材料的研发提供了新途径.     

Inhibition of Triton X-100／n-C5H11OH／H2O System on Hydrolysis of Cephanone

The hydrolysis of cephanone in Triton X-100 micelle and Triton X-100/n-C5H11OH/H2O(O/W) microemulsion were studied by means of UV-Vis absorption spectroscopy. The results show that compared with water, Triton X-100 micelle and Triton X-100/n-C5H11OH/H2O (O/W) microemulsion can inhibit the hydrolysis of cephanone. The inhibition effects of Triton X-100 micelle and Triton X-100/n-C5H11OH/H2O (O/W) microemulsion on the hydrolysis of cephanone are related to the location of cephanone in the interphases of Triton X-100 miceUes and Triton X-100/n-C5H11OH/H2O(O/W) microemulsion droplets.     

头孢唑酮与Triton X-100缔合体系的相互作用

抗生素头孢唑酮的加入使得非离子表面活性剂Triton X-100的表面活性降低. ¹H-NMR的结果表明，头孢唑酮增溶于胶束极性基团附近.头孢唑酮与Triton X-100胶束的结合常数随Triton X-100含量的增加而下降，但头孢唑酮在Triton X-100胶束相和水连续相之间的分配系数不随Triton X-100含量变化而变化.     

醇的链长对Triton X-100微乳液的调控

醇的链长对Triton X-100微乳液的调控;醇的链长;ＴＸ １００;Ｗ／Ｏ微乳液;纳米ＳｉＯ２;胶束数     

Synthesis of new 4,6-diaryl-2-(arylthio)nicotinonitriles in Triton X-100 aqueous micellar media

In this communication,we report four component condensations of acetophenone,arylaldehydes,arylthiol,and malononitrile in the presence of Triton X-100（5 mol%） aqueous micelles.This reaction led to the formation of 4,6-diaryl-2-（arylthio）nicotinonitrile new derivatives in good yields.The FT-IR,¹⁹F NMR,¹H NMR,¹³C NMR spectra and elemental analysis confirm the structure of compounds.     

Micelle-mediated cloud point extraction and spectrophotometric determination of rhodamine B using Triton X-100

A new micelle-mediated cloud point extraction method is described for sensitive and selective determination of trace amounts of rhodamine B by spectrophotometry. The method is based on the cloud point extraction of rhodamine B from aqueous solution using Triton X-100 in acidic media. The extracted surfactant rich phase is diluted with water and its absorbance is measured at 563 nm by a spectophotometer. The effects of different operating parameters such as concentration of surfactant and salt, temperature and pH on the cloud point extraction of rhodamine B were studied in details and a set of optimum conditions were obtained. Under optimum conditions a linear calibration graph in the range of 5-550 ng mL⁻¹ of rhodamine B in the initial solution with r = 0.9991 (n = 15) was obtained. Detection limit based on three times the standard deviation of the blank (3S_b) was 1.3 ng mL⁻¹ (n = 10) and the relative standard deviation (R.S.D.) for 50 and 350 ng mL⁻¹ of rhodamine B was 2.40 and 0.87% (n = 10), respectively. The method was applied for the determination of rhodamine B in soft pastel, hand washing liquid soap, matches tip and textile dyes mixture samples.     

发光金纳米粒子测定曲通X-100及其临界胶束浓度

参照文献方法合成了BSA保护的水溶性发光金纳米粒子, 并考察了此探针在非离子表面活性剂曲通X-100中的发光行为.根据观察到的发光增强效应, 建立了一种简单的测定曲通X-100的方法.考察了发光金纳米粒子的浓度、体系酸度、反应时间及共存物质对测定的影响.在最佳条件下, 发光强度与曲通X-100的浓度分别在0～150 μmol/L和150～600 μmol/L范围内分段成正比关系.两条工作曲线的交点所对应的浓度与曲通X-100的临界胶束浓度十分吻合, 为胶束形成过程提供了直接的指示.作为一种生物相容性探针, 发光金纳米粒子被用于生物学样品中曲通X-100的分析测定, 结果令人满意.     

Surface Property of Nonionic Surfactant Triton X-100 in an Ionic Liquid

A common nonionic surfactant Triton X-100 was dissolved in a commercial ionic liquid (IL) 1-butyl-3-methylimidazolium tetrafluoroborate (bmimBF₄). The surface tension of the bmimBF₄ solution was decreased with increasing the content of surfactant Triton X-100, a similar phenomenon with aqueous solution systems. Dynamic surface properties of Triton X-100 in bmimBF₄ were measured. It was found that pure IL solvents need rearrangement at the air-bmimBF₄ interface during the beginning stage of absorption. Moreover, the adsorption model was found to be in accord with the diffusion-controlled adsorption mechanism, and further, the dilute bmimBF₄ solutions are close to the diffusion-controlled adsorption.     

水/Triton X-100/正丁醇/环己烷反相微乳液体系中水结构的FT-IR研究

用FT-IR法研究了水/辛烷基苯酚聚氧乙烯醚（Triton X-100）/正丁醇/环己烷组成的反相微乳液体系水池中存在的束缚、结合和自由3种不同的水的状态.分别考察了水与表面活性剂物质的量之比（Rw）、水油比φ（水与环己烷的质量比）、助剂正丁醇的含量对3种状态水含量的影响.发现在φ=2.8、m正丁醇/mTriton X...     

A Study on the Location and Aggregation of Tetrahydrophenylporphyrin with a Single Hexadecyl Chain in Triton X-100 Micelle

The location and aggregation of 5,10,15-tris(4-hydroxyphenyl)-20-(hexadecyloxyphenyl)porphyrin (P) in nonionic polyoxyethylene (9.5) octylphenol (Triton X-100) micelle solutions were studied by means of UV–Vis and fluorescence spectra. P forms premicelle surfactant–porphyrin aggregates when the surfactant concentration is below and approaching the CMC. In Triton X-100 micelle solutions, different types of H-aggregates of P were formed when the concentration of P is higher than 3.9×10^-6?mol?dm^-3. As the bulk pH is changed, a transfer process for the porphyrin moiety in Triton X-100 micelle occurs. In neutral Triton X-100 micelle solutions, P may be located at the inner layer of the micelle; in basic conditions, the porphyrin moiety may transfer to the outer surface of the micelle. The kinetic study of porphyrin complexed with Cu(II) in Triton X-100 micelle solutions shows that the metalation rate could be controlled by changing the pH.