Thin-Layer Chromatographic Analysis of Flavonoids,Phenolic Acids,and Amino Acids in Some Croatian Hypericum Taxa

JPC – Journal of Planar Chromatography – Modern TLC - The flowering tops of Hypericum hirsutum, H. montanum, H. perforatum subsp. angustifolium, H. perforatum subsp. perforatum, and H....     

Analysis of PCB's by Thin-Layer Chromatography

Abstract

A screening thin layer chromatography (TLC) technique has been developed that can be done in the field. Quantitative analysis by TLC densitometry can be done in a laboratory (or field van). A densitometer is needed for quantitative work by TLC. An Apple computer may be added to use as a computational device. Semi-quantitative results may be obtained by visual comparisons. Confirmations may be made by silver halide visualization on TLC. Dehalogenation with sodium biphenyl, chlorination with antimony pentachloride, or mass spectral confirmation may be used. Quantitative analysis is possible of the derivatized positive samples.     

Microemulsion in Chemical Analyses by Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - This review encapsulates the work which appeared in the literature during 1995–2015 on the use of microemulsion as the mobile...     

Surfactants in Thin-Layer Chromatography

Data reported in the literature on the use of surfactants as modifiers of mobile and stationary phases in thin-layer chromatography are analyzed. The features of micellar and ion-pair versions of thin-layer chromatography and the dynamic and static modifications of stationary phases with surfactants are considered.     

Anodic Sampling of Titanium by Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - Titanium as the pure metal or as commercial titanium alloys has been widely used in dentistry. To determine the composition of...     

大豆磷脂组成的薄层色谱分析

采用国产硅胶板和二次展开的薄层色谱法，对具有重要的生物功能的大豆磷脂组成进行了分析，获得了大豆磷脂中１１个重要组分的定性结果。用已知样品量的薄层光密度扫描方法建立了卵磷脂定量方法，线性关系良好，并用于实际样品的测定，获得了满意结果。     

蔗糖酯的薄层分析

 以硅胶G板为固定相、甲苯 乙酸乙酯 甲醇 水 (体积比为 1 0∶5∶4 5∶0 2 )为流动相 ,研究建立了蔗糖酯薄层分析方法。在蔗糖酯上行展开后 ,用脲 磷酸 水饱和正丁醇溶液显色 ,斑点呈蓝色 ,蔗糖单酯的Rf 值为 0 1 6 ,多酯的Rf 值为 0 38～ 0 93。在 70℃显色 2 0min的最佳条件下 ,蔗糖单酯的检测量为 2 5 μg～ 2 50 μg时 ,其斑点面积与其检测量有良好的线性关系。用归一法和外标法对该分析方法的准确度进行考察和认证 ,两种方法对已知单酯含量的S 1 570样品测定结果的t 检验结果分别为 |t| =0 62 7(<2 571 )和 |t|=1 1 2 3(<2 571 ) 。     

薄层色谱法分离二元羧酸

利用薄层色谱法分离了二元羧酸,结果令人满意。     

Analysis of Bromo-DragonFLY by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC -     

N-二氯乙酰基噁唑烷合成反应的薄层色谱法研究

用薄层色谱法监测了用二氯乙酰氯、醇胺和酮合成的除草剂的安全剂N 二氯乙酰基 口恶唑烷的反应过程。方法操作简便、快速、准确。     

Triterpene Glycosides Analyzed by Two-Dimensional Thin-Layer Chromatography

Two-dimensional TLC with judiciously chosen solvent systems that differ in pH values is proposed for identification of triterpene glycosides in plant extracts (mono- and bisdesmosidic, acidic, sulfated, acylated)     

Quality Control of Commercial Mustard by Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - Horseradish samples (Cochlearis armoracia L.) and mustard samples (Sinapis alba L.) were extracted with 70:30 (v/v)...     

酰胺反应的薄层色谱法分析

用薄层色谱法监测了二氯乙酰氯与相应的胺合成二氯乙酰胺的反应过程,方法操作简便、快速、准确。     

Determination of the Antioxidant Activity of Juices by Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - The discovery of the implication of free radicals in the etiology of many diseases has led to increased interest in functional food...     

Analysis of Ebastine in Pharmaceutical Preparations by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC -     

Determination of Biphenyl in Citrus Fruits by Quantitative Thin-Layer Chromatography

Abstract

Residues of the fungicide biphenyl in citrus fruits have been determined by direct scanning of spots on phosphor-impregnated high performance silica gel TLC plates under UV light. Biphenyl was separated from fruit tissue by steam liquid-liquid extraction. Recoveries from spiked samples ranged from 92–99% at 100, 50, and 10 ppm levels. The precision of the TLC determination and overall procedure are shown to be adequate for residue analysis.     

Quantification of Lamotrigine in Human Serum by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - An HPTLC method with densitometric detection at 280 nm has been established for quantification of lamotrigine in human serum....     

Isolation and Identification of Amphetamines in Urine by Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC -     

薄层色谱法分析葵花仁粕中的绿原酸

 以聚酰胺薄膜为固定相、体积分数为 36 %的乙酸溶液为流动相 ,研究建立了薄层色谱分离测定葵花仁粕中绿原酸的方法。绿原酸样品溶液上行展开 8 5cm ,其Rf 值为 0 6 1。绿原酸的检测量在 0 0 5 μg～ 0 6 μg范围内 ,其斑点的面积与绿原酸的检测量具有良好的线性关系。绿原酸的回收率为 97 5 3 % ,不同薄层板之间的RSD为2 5 7% ,最低检出限为 0 0 2 5 μg。在以体积分数为 70 %的乙醇溶液为萃取剂、搅拌振荡萃取时间为 30min的条件下 ,以该方法测定萃取液中绿原酸的含量和葵花仁粕中绿原酸的残留量 ,确定了最佳萃取次数。     

Analysis of Flavonoids and Phenolic Acid in Propolis by Capillary Electrophoresis

A high-performance capillary electrophoresis (CE) is developed for the determination of six ingredients – rutin, ferulic acid, apigenin, luteolin, quercetin and caffeic acid – in Propolis and its extract capsules in this work. The effects of several factors such as the acidity and concentration of the running buffer, the separation voltage, the injection time and the wavelength of UV detector were investigated to find the optimum conditions. Under the optimum conditions, the analytes were separated within 14 min in a borax buffer (pH 9.2). Notably, excellent linearity was obtained over two orders of magnitude with detection limits (S/N=3) ranged from 3.7 × 10^–7 to 1.8 × 10^–6 g mL^–1 for all six analytes. The relative standard deviations of the migration times of six constituents ranged between 1.06% and 1.53%. The recoveries of six constituents ranged from 94.9% to 108.4%. This method was successfully used in the analysis of Propolis and its extract capsules with a relatively simple extraction procedure, and the assay results were satisfactory.