High-Performance Thin-Layer Chromatographic Quantification of Kaempferol and Apigenin in the Whole-Plant Powder of Sida spinosa Linn

JPC – Journal of Planar Chromatography – Modern TLC - Sida spinosa Linn. (synonyms: S. alba L., S. angustifolia Lam., S. angustifolia Mill.), Malvaceae, is an annual erect, branched...     

Estimation of Alprazolam and Sertraline in Pure Powder and Tablet Formulations by High-Performance Liquid Chromatography and High-Performance Thin-Layer Chromatography

Abstract

This article describes validated high-performance liquid chromatographic (HPLC) and high-performance thin-layer chromatographic (HPTLC) methods for simultaneous estimation of alprazolam (ALZ) and sertraline (SER) in pure powder and tablet formulation. The HPLC separation was achieved on a Nucleosil C18 column (150 mm long, 4.6 mm i.d., and 5-µm particle size) using acetonitrile and phosphate buffer (50 + 50 v/v), pH 5.5, as the mobile phase at a flow rate of 1.0 mL/min at ambient temperature. The HPTLC separation was achieved on an aluminum-backed layer of silica gel 60 F₂₅₄ using acetone/toluene/ammonia (6.0:3.0:1.0, v/v/v) as the mobile phase. Quantification with the HPLC method was achieved with ultraviolet (UV) detection at 230 nm over the concentration range 3–18 µg/mL for both drugs with mean recovery of 101.86 ± 0.21 and 100.57 ± 0.31% for ALZ and SER, respectively. Quantification in HPTLC was achieved with UV detection at 230 nm over the concentration range of 400–1400 ng/spot for both drugs with mean recoveries of 101.32 ± 0.15 and 100.38 ± 0.51% for ALZ and SER, respectively. These methods are rapid, simple, precise, sensitive, and are applicable for the simultaneous determination of ALZ and SER in pure powder and formulations.     

Analysis of Bromo-DragonFLY by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC -     

Quantification of Eugenol in Cinnamomum tamala Nees and Eberm. Leaf Powder by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - Eugenol is used as a flavor in the food industry, has a variety of biological activity, and can serve as a biomarker. Because...     

Pharmacokinetic Analysis of Ursolic Acid in Alstonia scholaris R. Br. by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - A simple and rapid high-performance thin-layer chromatographic method has been established and validated for determination of...     

High-Performance Thin-Layer Chromatography Combined with Densitometry for Quantitative Analysis of Chlorogenic Acid in Fruits of Peucedanum alsaticum L.

JPC – Journal of Planar Chromatography – Modern TLC - High-performance thin-layer chromatography with densitometric detection has been used for quantitative analysis of chlorogenic acid...     

Quantification of Puerarin in Pueraria tuberosa DC. by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC -     

Densitometric Simultaneous Quantification of Three Naphthoquinones from Impatiens balsamina L. Leaves by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - Impatiens balsamina belonging to family Balsaminaceae is commonly known as terda. The plant has extensive range of...     

Analysis of Ebastine in Pharmaceutical Preparations by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC -     

Quantification of Lamotrigine in Human Serum by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - An HPTLC method with densitometric detection at 280 nm has been established for quantification of lamotrigine in human serum....     

Simultaneous Determination of Pioglitazone and Glimepiride by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC -     

Quantitative Determination of Propranolol in Human Serum by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - A high-performance thin-layer chromatography (HPTLC) method for the quantification of propranolol in human serum is developed and...     

Simultaneous Quantitation of Piperine and Piperlongumine in the Fruit of Piper longum Linn. by Validated High-Performance Thin-Layer Chromatography—Densitometric Method

JPC – Journal of Planar Chromatography – Modern TLC - The fruit of Piper longum Linn. (family: Piperaceae), known as pippali in India, is a reputed drug of Ayurveda (the Indian system...     

薄层色谱法分离二元羧酸

利用薄层色谱法分离了二元羧酸,结果令人满意。     

High-Performance Thin-Layer Chromatography and Reversed-Phase High-Performance Liquid Chromatography Methods for Fingerprinting of Salvadora persica Root Powder Extract Using Benzyl Isothiocyanate as Biomarker

JPC – Journal of Planar Chromatography – Modern TLC - Salvadora persica plant having a number of antimicrobial substances and the roots of the S. persica shrub have been demonstrated to...     

Rapid Quantitation of Verapamil in Plasma by High-Performance Liquid Chromatography

Abstract

A simple and sensitive liquid chromatographic assay procedure using a fluorescence detector for the quantitative determination of verapamil in plasma without extraction was developed. After precipitating the protein with acetonitrile, the resulting supernatant liquid was injected onto the column for analysis. Chromatographic separation was achieved on C₁₈ reversed phase column and the eluting solvent was the isocratic mixture of methanol, acetonitrile and pH 3.0 glycine buffer (1:4:5). With this mobile phase the drug and its internal standard were well separated from the interference of the plasma sample. The average recovery of verapamil from 3 replicate samples of different concentration (100–600 ng/mL) were 95.5 ± 5.68%. The minimum amount of verapamil detectable by this method was 40 ng/mL of sample. The elimination half-life (β-phase) of this drug in rabbits was found to be 3.7 hours.     

Simultaneous Densitometric Determination of Rifampicin and Isoniazid by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - High-performance thin-layer chromatography (HPTLC) on silica gel 60 F254 TLC plates, with ethyl acetate-methanol-acetone-acetic...     

Simultaneous Densitometric Determination of Aspirin and Omeprazole by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - A new, simple, accurate, and precise high-performance thin-layer chromatographic (HPTLC) method has been developed for the...     

Simultaneous Densitometric Determination of Ivermectin and Albendazole by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - This paper describes a validated high-performance thin-layer chromatography (HPTLC) method for the simultaneous determination of...     

Quantification of Dopamine in Portulaca oleracea Linn,by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - A sensitive and accurate high-performance thin-layer chromatographic method has been developed, validated, and used for...