Isolation and Densitometric HPTLC Method for Quantification of Belleric Acid in the Fruit Pericarp of Terminalia bellerica and its Formulations

JPC – Journal of Planar Chromatography – Modern TLC - For the first time a simple, sensitive, rapid, precise and accurate HPTLC method has been established for quantification of...     

UV Densitometric HPTLC Method for Analysis of Nitrazepam in Pharmaceutical Formulations

JPC – Journal of Planar Chromatography – Modern TLC - A UV densitometric high-performance thin-layer chromatographic (HPTLC) method for analysis of nitrazepam in pharmaceutical...     

Improved and Validated HPTLC Method for Quantification of Oenothein B and its Use for Analysis of Epilobium angustifolium L.

JPC – Journal of Planar Chromatography – Modern TLC - A selective and simplest possible high-performance TLC (HPTLC) method for quantification of oenothein B on the basis of the free...     

Simultaneous Determination of Caffeine and Paracetamol in Commercial Formulations Using Greener Normal-Phase and Reversed-Phase HPTLC Methods: A Contrast of Validation Parameters

There has been no assessment of the greenness of the described analytical techniques for the simultaneous determination (SMD) of caffeine and paracetamol. As a result, in comparison to the greener normal-phase high-performance thin-layer chromatography (HPTLC) technique, this research was conducted to develop a rapid, sensitive, and greener reversed-phase HPTLC approach for the SMD of caffeine and paracetamol in commercial formulations. The greenness of both techniques was calculated using the AGREE method. For the SMD of caffeine and paracetamol, the greener normal-phase and reversed-phase HPTLC methods were linear in the 50–500 ng/band and 25–800 ng/band ranges, respectively. For the SMD of caffeine and paracetamol, the greener reversed-phase HPTLC approach was more sensitive, accurate, precise, and robust than the greener normal-phase HPTLC technique. For the SMD of caffeine paracetamol in commercial PANEXT and SAFEXT tablets, the greener reversed-phase HPTLC technique was superior to the greener normal-phase HPTLC approach. The AGREE scores for the greener normal-phase and reversed-phase HPTLC approaches were estimated as 0.81 and 0.83, respectively, indicated excellent greenness profiles for both analytical approaches. The greener reversed-phase HPTLC approach is judged superior to the greener normal-phase HPTLC approach based on numerous validation parameters and pharmaceutical assays.     

Validated HPTLC Method for Quantification of Vitamin D3 in Fish Oil

JPC – Journal of Planar Chromatography – Modern TLC - A selective high-performance thin-layer chromatographic (HPTLC) method for the quantification of vitamin D3 was developed and...     

HPTLC Quantification of Some Flavonoids in Extracts of Satureja hortensis L. Obtained by Use of Different Techniques

JPC – Journal of Planar Chromatography – Modern TLC - A study of quantitative analysis of some flavonoids in Satureja hortensis L. extracts is presented. The HPTLC analyses were...     

Development and Validation of HPTLC Densitometric Method for Identification and Quantification of Geraniol in Palmarosa Oil

JPC – Journal of Planar Chromatography – Modern TLC - India is the main producer of palmarosa oil obtained from rosha grass (Cymbopogan martini var. motia) of family Graminae. The...     

Simultaneous Separation and Determination of Lamivudine and Zidovudine in Pharmaceutical Formulations Using the HPTLC Method

Abstract

A high-performance thin-layer chromatographic method (HPTLC) for the simultaneous determination of lamivudine and zidovudine in a binary mixture has been developed. The method developed was based on HPTLC separation of the two drugs followed by densitometric measurements of spots at 276 and 271 nm for lamivudine and zidovudine, respectively. Separation was carried out on Merck HPTLC silica-gel 60 F₂₅₄ plates, using toluene/chloroform/methanol (1:6:3 v:v) as the mobile phase. Validation of the method was performed based on The International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use (ICH) guidelines in terms of linearity, accuracy, precision, limit of detection, limit of quantification, and robustness. Second-order polynomial equations were obtained for the regression line in the ranges of 250–1400 and 250–1700 ng/spot for lamivudine and zidovudine respectively. Correlation coefficient (r) values were 0.9998 for both analytes. The method provides sufficient accuracy as indicated by recovery percentages given for lamivudine and zidovudine. For system precision study, the low coefficient of variation values (<2%) for both lamivudine and zidovudine ensured reproducible performance of the instrument. In the method precision study, coefficients of variation <2% were obtained, which showed that the proposed method provides acceptable intraday and interday variation. The detection and quantification limits and were 3.06 and 9.28 ng/spot for lamivudine and 3.34 and 10.13 ng/spot for zidovudine, respectively. Parameters such as mobile-phase composition, volume of mobile phase, time from spotting to development, and time from development to scanning were employed while testing for robustness of the method, and the standard deviation of peak areas was calculated for each parameter. The low coefficient of variation values indicated the robustness of the method. Statistical manipulation did not show any significant effect of one parameter over the others on the robustness of the method.     

Quantification of Valerenic Acid in Valeriana jatamansi and Valeriana officinalis by HPTLC

A simple, rapid, cost-effective and accurate high performance thin layer chromatographic method has been developed for quantification of valerenic acid in Valeriana jatamansi and Valeriana officinalis which is one of the stable compounds of Valeriana officinalis and designated as a key marker compound. Valerenic acid makes substantial contribution to the sedative and spasmolytic activity of the essential oil and extract of Valeriana officinalis. Separation and quantification was achieved by HPTLC using ternary mobile phase of hexane: ethyl acetate: acetic acid (80:20:0.5 v/v) on precoated silica gel 60F₂₅₄ aluminium plates and densitometric determination was carried out after derivatization with anisaldehyde-sulphuric acid reagent at 700 nm, in absorption-reflectance mode. The calibration curves were linear in the range of (500 ng–2.5 μg). This is the first HPTLC report for the identification and quantification of valerenic acid in Valeriana jatamansi and Valeriana officinalis.     

Densitometric HPTLC Method for Simultaneous Quantification of Sennosides A and B and Gallic Acid in a Pharmaceutical Dosage Form

JPC – Journal of Planar Chromatography – Modern TLC - A densitometric HPTLC method has been established for simultaneous quantification of sennosides A and B and gallic acid in a...     

A Rapid Densitometric Method for the Quantification of Luteolin in Medicinal Plants Using HPTLC

Luteolin, a flavonoid, is reported to occur widely in many medicinal plants. It has been shown to have important biological activities. We report sensitive HPTLC method for the quantification of luteolin from plant material. The method was validated for precision, repeatability and accuracy. The method was found to be precise with RSDs for intraday in the range of 0.77–1.29% and inter–day in the range of 1.02–2.08%. Instrumental precision and repeatability of the method were found to be 0.39 and 0.57 (%CV). Accuracy of the method was checked by recovery study conducted at two different levels and the average percentage recovery was found to be 100.92%. The method was used for quantification of luteolin in three important herbal drugs viz. fruit of Cuminum cyminum, whole plant of Bacopa monnieri, flower of Achillea millefolium. The proposed HPTLC method for the quantification of luteolin was found to be simple, precise, specific, sensitive and accurate and can be used for quality control of raw materials.Revised: 1 October 2003 and 18 February 2004     

An Improved Narrow-Bore LC Method for Quantification of Alfuzosin in Pharmaceutical Formulations

A simple rapid and stability-indicating LC method using a narrow-bore column has been developed, fully validated, and applied to the quantification of alfuzosin in pharmaceutical formulations. Chromatography was achieved isocratically on a narrow-bore, 5-μm particle size, C₈ analytical column. The mobile phase was a 35:65 (v/v) 0.0125 m ammonium formate–acetonitrile at a flow rate of 0.35 mL min^?1. Detection was by UV absorption at 245 nm. Evaluation over the range 200–800 ng mL^?1 revealed linearity was good. Limits of detection and quantification for alfuzosin were 22.9 and 69.5 ng mL^?1, respectively. Intra-day and inter-day RSD were less than 6.4%, and the relative percentage error was less than ?1.7% (n = 5). Accelerated degradation performed under different stress conditions including oxidation, hydrolysis, and heat, proved the selectivity of the procedure. The method was successfully used for quality-control and content-uniformity testing of commercial tablets.     

HPTLC Method for Quantification of Isovitexin in the Whole-Plant Powder of Enicostemma littorale Blume

JPC – Journal of Planar Chromatography – Modern TLC - A sensitive and accurate high-performance thin-layer chromatographic method has been developed, validated, and used for...     

Application of Videodensitometric and Classical Densitometric HPTLC for Quantification of Fluoroquinolone Derivatives in Pure and Tablet Forms

JPC – Journal of Planar Chromatography – Modern TLC - A new, simple, and rapid high-performance thin layer chromato-graphic method coupled with densitometry has been developed and...     

Determination and Quantification of Pitavastatin Calcium in Tablet Dosage Formulation by HPTLC Method

Abstract

A simple, sensitive, reliable, and rapid HPTLC method has been developed for the determination of pitavastatin calcium in tablet dosage form. Identification and determination were performed on aluminum backed silica gel 60F₂₅₄ washed with methanol. The mobile phase of ethyl acetate‐methanol‐ammonia‐1 drop formic acid (7:2:0.8) calibration plots were established showing the dependence of response (peak area) on the amount chromatographed. The spot were scanned at 245 nm. The method has a linear range of 50–250 ng/spot. The method was validated for selectivity, repeatability, and accuracy. The method was used for determination of the compound in commercial pharmaceutical dosage forms. It is a more effective option than other chromatographic techniques in routine quality control.     

Stability-Indicating Densitometric HPTLC Method for Qualitative and Quantitative Analysis of Hydroquinone in Commercial Whitening Creams

JPC – Journal of Planar Chromatography – Modern TLC - A densitometric high-performance thin-layer chromatographic (HPTLC) method for analysis of hydroquinone has been developed and...     

Use of HPTLC to Establish the Chemotype of a Parasitic Plant,Dendrophthoe falcata (Linn. f.) Etting. (Loranthaceae), Growing on Different Substrates

JPC – Journal of Planar Chromatography – Modern TLC - Dendrophthoe falcata (Linn. f.) Etting. is a stem parasite commonly known as ‘Vanda’ in the Ayurvedic system of...     

HPTLC Method for Quantification of Apigenin in the Dried Root Powder of Gmelina arborea Linn.

JPC – Journal of Planar Chromatography – Modern TLC - A sensitive high-performance thin-layer chromatographic method has been established for quantification of apigenin in dried root...     

Stability-Indicating HPTLC Method for Analysis of Moclobemide, and Use of the Method to Study Degradation Kinetics

A sensitive, selective, precise, and stability-indicating HPTLC method for analysis of moclobemide in the bulk drug and in formulations has been established and validated. Aluminium TLC plates precoated with silica gel 60 F₂₅₄ were used with benzene–methanol–40% ammonia 7:3:0.1 (v/v) as mobile phase. Densitometric analysis was performed in absorbance mode at 238 nm. Compact bands were obtained for moclobemide (R _F 0.67 ± 0.02; n = 6). The drug was subjected to acidic and alkaline hydrolysis, oxidation, dry heat treatment, and photodegradation. The drug undergoes degradation under acidic, basic, and oxidising conditions. The degradation products were well resolved from the pure drug with significantly different R _F values, so the method can be regarded as stability-indicating. Response to moclobemide was a linear function of amount in the range 50–600 ng per band, with a correlation coefficient, r ², of 0.9967 ± 0.51. LOD and LOQ, determined experimentally, were 10 and 30 ng per band, respectively. Statistical analysis proves the method is repeatable and specific for analysis of moclobemide. The method was used to investigate the kinetics of alkaline degradation. The Arrhenius plot was constructed and the activation energy calculated.     

Standardization of Crude Extract of Neem Seed Kernels (Azadirachta indica A. Juss) and Commercial Neem Based Formulations Using HPTLC and Extended Length Packed-Columns SFC Method

Two chromatographic techniques are described for the separation and quantitative determination of azadirachtin A and B, salannin, and nimbin present in the crude extract of neem seed kernels and commercial neem based formulations. The high performance thin-layer chromatography (HPTLC) separation of markers was carried out on Merck TLC aluminium sheets of silica gel 60 F254 using ethylacetate-benzene (7.0: 3.0, v/v) as mobile phase. The other technique was based on extended length packed column supercritical fluid chromatographic (PC-SFC) separation of the markers using Cyano column (250 mm × 4.6 mm I.D, S-5.0 μ) and Kromasil 100 NH₂ column (250 mm × 4.6 mm I.D, 5.0 μ) connected in series. The detection was carried out using photodiode array detector at 338 K using methanol-modified carbon dioxide (10%) as the mobile phase at flow rate of 2.0 mL min⁻¹. The current study assesses the effect of extending column length during PC-SFC experiment to obtain maximum resolution between a number of unknown components and known markers present in neem seed extracts. Both the chromatographic methods were validated in terms of precision, robustness, recovery, limits of detection and quantitation. The analysis of variance (ANOVA) and Student’s t-test were applied to correlate the results of quantitative determination of markers by means of HPTLC and PC-SFC method.