Radikale aus Polymethin-Farbstoffen. IV—Dikation-Radikale aus Polymethincyanin-Farbstoffen mit Indolenin-Endgruppen

Dication radicals have been prepared by electrolytic oxidation of polymethinecyanine dyes. Their e.s.r. spectra confirm the expected structure of the radicals.     

Radikale aus Polymethincyanin-Farbstoffen. I—Polymethincyanine mit Indolenin-Endgruppen

The solution ESR spectra of radicals which have been prepared by electrolytic reduction of dinuclear and trinuclear polymethinecyanine dyes are interpreted. From the values of the coupling constants information can be obtained about the conformation of the radicals.     

Reinigung der D-Oxynitrilase aus Mandeln mit Hilfe der Affinitäts-Chromatographie

A Rapid Purification of D -Oxynitrilase from Almond Meal by Affinity Chromatography Oxynitrilase from almond meal is capable of catalyzing the stereospecific addition of cyanide to a variety of aldehydes. Thus, the enzyme is potentially useful in the synthesis of optically active cyanohydrins on a preparative scale [1]. As the currently available purification procedures for this enzyme [2] are rather tedious, we have elaborated a simple and rapid procedure based on affinity chromatography. An inhibitor for the enzyme, methyl p-(3-aminopropoxy)benzoate (4) , has been synthesized and attached covalently to Sepharose 4B as a solid matrix (5) . With this affinity gel it was possible to prepare the D -oxynitrilase in a simple procedure with high yields.