纸上光度法间接测定痕量硫离子

用过量的铜标准溶液与含S2-样品溶液作用,加入适量的二氯甲烷,使生成的硫化铜微晶滞留在有机相中,用纸上光度法测定残留在水相中的痕量Cu2+,间接测得样品中S2-的含量。测定的pH范围为3～6,S2-的测定范围为3×10-6～9×10-6mol·L-1,RSD小于3%。     

分光光度法测定水样中的硫离子

A new simple spectrophotometric method was proposed for the determination sulphur ion in water samples.In the medium of 0.05 mol/L HCl,sulphur anion turns mixed acid of the silicon and molybdenum into molybdenum blue.Its absorption maxima was found at the wavelength of 700 nm,with a molar absorptivity of 2.48×104L·mol-1·cm-1.Influencing factors such as the amount of reagent and reaction time were optimized,and the interferences from common aions were also investigated.This method was successfully used for t...     

萃取光度法间接测定硫

随着工业现代化的发展,硫对环境造成的污染越来越严重,因此,硫的测定受到越来越多的重视。目前硫离子的测定方法主要有亚甲基蓝光度法,流动注射分光光度法,动力学光度法,间接光度法和化学发光法等。用萃取光度法间接测定硫离子还未见报道。本文研究了在碱性介质中,利用S^2-和Cu（Ⅱ）生成硫化铜沉淀,从而抑制Cu（Ⅱ）和二苯卡巴腙（DPCZ）显色反应进行的程度。     

meso—四（4—溴苯基）卟啉分光光度法测定烟草中的痕量铅的研究

本研究了在ＰＨ９．０和有Ｔｗｅｅｎ－８０存在下非水溶性试剂ｍｅｓｏ四（４－溴苯基）卟啉与铅的张合反应条件，络合物的最大吸收波长为４６８ｎｍ，组成摩尔比为１：１，铅含量在０－２４μｇ／２５ｍＬ范围内有较好的线性关系，表观摩尔吸光系数为２．８７×１０＾５，用于烟草中痕量铅的测定，结果令人满意。     

卟啉试剂与稀土元素显色反应的分光光度法研究（Ⅰ）meso－四（对磺基苯）卟啉（TPPS4）分光光度法测定痕量钇

本文研究了在ｐＨ１１．０的硼砂－ＮａＯＨ缓冲介质中，以Ｈｇ（Ⅱ）作催化剂，在乳化剂（ＯＰ）和溴化十六烷基三甲基铵（ＣＴＭＡＢ）协同作用下，ｍｅｓｏ－四（对磺基苯）卟啉（ＴＰＰＳ４）测定钇（Ⅲ）的显色反应，其摩尔吸光系数ε４１７为５．４６×１０６Ｌ·ｍｏｌ－１·ｃｍ－１，钇浓度在０～０．０３０μｇ／１０ｍＬ内符合比耳定律，在室温下显色迅速，１００倍重稀土不干扰，测定合成稀土混合样品中钇（Ⅲ）含量，回收率为９４％～９８％．     

流动注射-分光光度法测定催化氧化水样中的硫离子

1　引　　言以粉煤灰作催化剂催化Ｈ2 Ｏ2 氧化硫化钠溶液的方法处理水样时 ,为了评价氧化处理的效果 ,对处理过的水样中Ｓ2 - 离子含量的准确测定特别重要。目前 ,测定硫化物的光度法有间接光度法、亚甲基蓝比色法等 ,但由于方法的再现性差、灵敏度低及其操作繁琐而不适宜于快速测定。由于流动注射光度法具有快速、灵敏和简便等特点而日益受到重视。迄今为止 ,仅有少数流动注射光度分析测定汞和亚硝酸盐等的文献 ,还未见用于Ｓ2 - 离子测定的报道。本文采用在ＨＣｌ溶液中以Ｎ ,Ｎ 二甲对苯二胺二盐酸盐 (ＤＭＰＤ)和ＦｅＣｌ3为试剂 ,利用…     

四（4—甲氧基—3—磺酸苯基）卟啉分光光度法测定微量铅

研究了水溶性卟啉５，１０，１５，２０－四（４－甲氧基－３－磺酸苯基）卟啉（Ｔ（４－ＭＯＰ）ＰＳ４）与铅的显色反应，在ｐＨ１１和酒石酸钾存在下铅与Ｔ（４－ＭＯＰ）ＰＳ４在室温时间形成１：１的配合物，其最大吸收波长在位于４６４．４ｎｍ，摩尔吸光系数为２．３×１０＾５Ｌ．ｍｏｌ＾－１．ｃｍ＾－１，铅量在０～２０μｇ／２５ｍＬ，范围为符合比尔定律，工作工线回归方程Ａ＝０．０２６２＋０．０４３５ｃ相关系数ｒ     

meso—四（4—溴苯基）卟啉与Ag（I）的显色反应光度法研究

研究了ｐＨ１０．０，Ｔｗｅｅｎ－８０存在时，ｍｅｓｏ－四（４－溴苯基）卟啉与Ａｇ（Ｉ）络合显色的反应条件，络合物的最大吸收波长为４２７ｎｍ，表观摩尔吸光系数为３．５×１０５，Ａｇ（Ｉ）在０～０．４８ｍｇ／Ｌ范围内呈线性关系，回归方程为：Ａ＝０．１３１Ｃ＋０．００１２，相关系数γ＝０．９９９８。此法用于纯铅样和照相废液中痕量银的测定，结果与ＡＡＳ结果相比令人满意     

催化动力学光度法测定痕量硫离子

基于氢氧化钠溶液中，Ｓ~（２－）对Ｈ_２Ｏ_２氧化Ｉ－的反应有催化作用，固定反应时间，将此催化体系与二苯胺碘酸钠氧化显色反应相偶合，建立了催化动力学光度法测定痕量硫离子的新方法，并讨论了其动力学条件。方法灵敏度１．４×１０~（－１１）ｇ／ｍＬ，测定范围１．０～１２．０ｎｇ／ｍＬ。操作简便快速，用于测定天然水及含硫工业废水中的痕量硫离子，结果满意。     

褪色分光光度法间接测定草甘膦

在ｐＨ＝５．６的醋酸－醋酸钠冲溶液中，草甘膦与微量过量的溴反应，剩余的溴能使罗丹明Ｂ褪色，以此建立了一种分光光度法测定甘甘膦的新方法，在最佳条件下，草甘膦浓度与吸光度呈良好的线性关系，当加入溴的浓度分别为１．４４×１０＾－５ｍｏｌ／Ｌ和２．７２×１０＾－５ｍｏｌ／Ｌ时，测定范围分别为０～０．５ｍｇ／Ｌ和０．４～０．９ｍｇ／Ｌ，干扰试验表明Ｆｅ＾２＋，Ｆｅ＾３＋和三乙胺有严重干扰，但可被阳离子交换权     

Indirect Spectrophotometric Method for Determination of Sulfur Dioxide

Abstract

An operationally inexpensive and satisfactory analytical procedure for sulfur dioxide is proposed. The reagent 3-methyl-1,2-cyclopentanodione dithiosemicarbazone has been used to determine trace amounts of sulfur dioxide indirectly using the reduction of Fe(III) to Fe(II) principle. In order to find the optimal conditions for SO₂ determination, properties of 3-Me-CPDT-Fe(II) complex such as its composition, stability and free energy change of formation have been determined. The best conditions for the complex formation such as standing time, pH, wavelength and the effect of interfering ions are described. The complex has been used with success in spectrophotometric determination of SO₂. The procedure can determine down to 0.63 μg and recoveries are better than 97.5%. The method is also suitable for determination of sulfur dioxide in the air provided that interfering gases such as H₂S and NO₂ are removed.     

Spectrophotometric Determination of Diphenadione Via its Metal Complexes

Abstract

Spectrophotometric procedure is described for the quantitative determination of diphenadione [2-(diphenylacetyl)-1,3-indandione], based on direct spectrophotometric measurements of the absorbances of its iron (III), iron (II) and cobalt (II), metal complexes at 488 nm, 505 nm and (334 nm, 372 nm), respectively. The drug reacts with metals in the ratio of 3:1 and 2:1 for iron (III) and for both iron (II) and cobalt (II) respectively. The obtained complexes have apparent molar absorptivities of 1.48 × 10³ 1 mol^?1 cm^?1, 0.714 × 10³ 1 mol^?1cm^?1 and (1.70 × 10³ 1 mol^?1cm^?1, 1.93 × 10³ 1 mol^?1cm^?1) for iron (III), iron (II) and cobalt (II) complexes, respectively. The procedure is suggested for the determination of 51–400 μg.ml^?1 diphenadione via the iron (II) complex and 35–170 μg.ml^?1 diphenadione via both cobalt (II) and iron (III) complexes. The suggested procedure has accuracies of 99.79 ± 0.67%, 99.64 ± 0.37% and (100.09 ± 0.53%, 99.99 ± 0.42%) for the metal complexes of iron (III), iron (II) and cobalt (II), respectively.     

7-苯基偶氮-变色酸与蛋白质的显色反应研究

研究了蛋白质与 7-苯基偶氮 -1 ,8-二羟萘 -3 ,6-二磺酸的显色反应。在 p H2 .7的缓冲溶液中 ,7-苯基偶氮 -1 ,8-二羟萘 -3 ,6-二磺酸与蛋白质形成红紫色复合物 ,λmax为 564 nm,ε为 1 .3× 1 0 5L· mol- 1· cm- 1,线性范围为 6.6～ 1 3 2 mg/ L。实验证实7-苯基偶氮 -1 ,8-二羟萘 -3 ,6-二磺酸与蛋白质的作用符合 Pesavento模式。所提出的方法不经任何预处理可直接测定血清和花生中的蛋白质     

Spectrophotometric Determination of Nitrofurazone

Abstract

A new sensitive and seleactive method for rapid and accurated determination of nitrofarazone is described. The nitrofurazone is reacted with hydroxyl-amine hydrochloride in alkaline medium to give hydroxamic acid which forms the purple violet coloured complex with iron (III), FeCl₃, in acidic medium. The nitrofurazone was determined in presence of commonly used drugs and additives. The results obtained by the proposed and B.P. methods were compared.     

锌试剂分光光度法测定壳聚糖含量

利用锌试剂与壳聚糖在一定酸度条件下的特异性显色反应，即其复合物在465nm处吸光度与壳聚糖含量在0～O．04g／L范围内呈现良好的线性关系(R=0．996)，基于此建立了一种简便快速的分光光度法。平均回收率为99．68％。     

在线分离光度法测定痕量金的研究

本提出了一种泡沫塑料柱在线分离富集／β－环糊精－十二烷基苯磺酸钠溶液在线洗脱／硫代米蚩酮光度法测定痕量金的新方法。方法的检测出限是３×１０＾－＾８ｇＡｕ，线性范围是５×１０＾－＾７－８×１０＾－＾６Ａｕ，对于１．０μｇＡｕ进行１１次测定，相对标准偏差小于３．０％。     

蛋白质的铬偶氮KS分光光度法测定

在 pH1.4的克拉克 -鲁布斯(Clark -Lubs)缓冲介质中 ,红色的铬偶氮KS能与蛋白质作用形成紫色的复合物 ,其最大吸收波长为588nm ,比铬偶氮KS本身红移68nm ,测定的表观摩尔吸光系数ε588 达2.971×105 L/(mol·cm)(牛血清白蛋白) ,线性范围为10～140mg/L;所拟方法用于人血清中总蛋白的测定 ,结果与考马斯亮蓝G_250法一致     

溴甲酚绿分光光度法测定麻黄碱含量的研究

建立了溴甲酚绿分光光度法测定麻黄碱的方法。该方法最大吸收波长为620nm,摩尔吸光系数为1.78×104L.mol-1.cm-1,盐酸麻黄碱含量在20~120μg/mL范围内遵守比耳定律。用该法测定麻黄饮片及麻杏止咳片中的麻黄碱,平均回收率分别为98.9%、101.2%(n=3),相对标准偏差(RSD)<2.31%。方法简单、快速,适用于生药及产品的质量控制。     

Spectrophotometric Determination of Formaldehyde

ABSTRACT

A simple and very sensitive method for the determination of formaldehyde is described. Formaldehyde reacts with phloroglucinol in acid medium producing a yellow dye with λ_max at 435nm. The method is useful for the determination of formaldehyde in the range 0-2.5μg in an overall volume of 10mL with molar absorptivity of 8.3 × 10⁴1 mol^?1 cm^?1 and a relative standard deviation of 2% at 1.5μg level (n=10).