用于磁性靶向治疗肺纤维化的聚多巴胺包覆的Fe3O4/甲强龙/环磷酰胺复合超粒子

肺纤维化是一种致命性肺部疾病, 目前临床常规的甲强龙(MPS)联合环磷酰胺(CTX)治疗方法存在明显的不良反应. 基于降低药物毒副作用的目的, 本文设计了一种聚多巴胺(PDA)包覆的Fe₃O₄纳米粒子/甲强龙/环磷酰胺复合超粒子(Fe₃O₄/MPS/CTX@PDA SPs), 提出磁性靶向治疗肺纤维化的思路. 从预制的油溶性Fe₃O₄纳米粒子出发, 通过水包油微乳液模板法制备了Fe₃O₄ 超粒子(SPs), 并在进一步包覆PDA壳层的过程中引入MPS和CTX, 制备了Fe₃O₄/MPS/CTX@PDA SPs, 考察了Fe₃O₄/MPS/CTX@PDA SPs的稳定性、 磁性、 对MPS和CTX的负载及释放, 分析了其生物毒性, 并建立动物模型验证了其磁性靶向功能.     

羧甲基纤维素/Fe3O4复合纳米磁性材料的制备、表征及吸附性能的研究

采用改进的氧化沉淀法在羧甲基纤维素(CMC)体系中制备了以磁性纳米Fe₃O₄为核心, 外层包覆羧甲基纤维素的复合磁性纳米材料. 用透射电镜、X射线衍射、红外光谱、Zeta电位和震动样品磁强计对复合纳米Fe₃O₄进行了表面形貌、结构和磁学的表征. 在此基础上研究了复合纳米Fe₃O₄对Cu^2＋的吸附性能, 探讨了溶液pH、反应时间和 Cu^2＋的初始浓度对其吸附性能的影响. 实验结果表明, 复合Fe₃O₄粒子为反尖晶石型, 平均粒径在40 nm左右, 羧甲基纤维素在Fe₃O₄粒子表面是化学吸附, 复合Fe₃O₄粒子的饱和磁化强度为36.74 emu/g, 在中性溶液中Cu^2＋的吸附量最高, 吸附平衡时间为1.5 h, 二级动力学模型能够很好地拟合吸附动力学数据, 吸附等温数据符合Langmuir模型. 复合纳米Fe₃O₄对Cu^2＋的吸附机理主要为表面配位反应.     

磁性印迹纳米粒子固定血红蛋白修饰磁性电极构建过氧化氢传感器

采用表面印迹技术,以磁性二氧化硅纳米粒子（Fe₃O₄@SiO₂ NPs）作为载体、血红蛋白（Hb）为模板分子、正硅酸乙酯（TEOS）为印迹聚合物单体,制备了Hb印迹Fe₃O₄@SiO₂的磁性印迹纳米粒子（MMIPs NPs）. MMIPs NPs具有磁性内核和血红蛋白印迹壳层的核壳结构,可以富集并固定Hb. 使用壳聚糖将MMIPs NPs固定于磁性电极表面,构建血红蛋白类酶生物传感器,研究了Hb对过氧化氢（H₂O₂）的催化活性. MMIPS NPS相比于磁性非印迹纳米粒子（MNIPS NPS）,催化电流增加了14.3%. 采用磁性电极,MMIPS NPS、Hb和O₂的顺磁性使得该类酶生物传感器对H₂O₂的催化电流增加了60.0%. 血红蛋白类酶生物传感器电流响应与H₂O₂浓度在25 ~ 200 μmol·L^-1间呈线性关系,检出限为3 μmol·L^-1（S/N=3）,表明该类酶传感器对H₂O₂具有良好的催化性能.     

纳米Fe2O3/端羟基聚丁二烯(HTPB)复合粒子的制备与表征

为改善纳米Fe₂O₃在固体推进剂中的分散性, 以端羟基聚丁二烯(HTPB)和异氟尔酮二异氰酸酯(IPDI)为包覆和固化材料, 分别采用直接共混法和分步共混法制得纳米Fe₂O₃/HTPB复合粒子. 采用HRTEM, TGA, FTIR和XRD等手段表征了复合粒子的结构, 对比了复合前后纳米Fe₂O₃的分散性, 测量了HTPB包覆量和包覆层厚度. 结果表明, 这两种方法均能实现HTPB对纳米Fe₂O₃物理包覆, 但分步共混法明显优于直接共混法|在分步共混法制得的纳米Fe₂O₃/ HTPB复合粒子中, 内层的纳米Fe₂O₃分散性好, 外层HTPB厚度均一. 复合粒子亲油性结果表明, 将纳米Fe₂O₃与HTPB进行复合, 可有效改善纳米Fe₂O₃在固体推进剂中的分散性.     

磁光Fe3O4@SiO2@CdS复合材料的制备及表征

采用水解三氯化铁得到Fe₃O₄磁核,然后水解四乙氧基硅烷(TEOS)得到Fe₃O₄@SiO₂,再在其表面包覆半导体CdS即可得到良好的磁性光电信标Fe₃O₄@SiO₂@CdS。材料形貌、组成分析及光电化学测试表明成功地合成了具有超顺磁性的Fe₃O₄@SiO₂@CdS纳米复合材料。该材料分散均匀、具有良好的光学性能、在光电化学传感及光电催化等领域具有良好的应用前景。     

基于Fe3O4@SiO2@Ag/GCE传感器检测土霉素

合成了具有核壳结构的Fe₃O₄@SiO₂纳米复合材料,在Fe₃O₄@SiO₂表面负载银纳米离子,制备了一种新型的Fe₃O₄@SiO₂@Ag复合材料,并采用玻碳电极(GCE)构建Fe₃O₄@SiO₂@Ag/GCE传感器,对土霉素(OTC)进行定量检测。优化了电解质溶液的种类和pH,Fe₃O₄@SiO₂@Ag用量以及富集时间和富集电位等实验条件。在最佳条件下,OTC浓度(c)与峰电流(I_p)在0.01～120μmol/L范围内呈线性关系,线性方程为I_p=0.9307c-0.0033,线性相关系数R²=0.9986,检出限为8.2 nmol/L。该传感器对牛奶中OTC的检测结果与国标法结果一致。     

介孔结构增强的Fe3O4超粒子@介孔SiO2的光热性能

采用湿化学法制备了多功能Fe₃O₄超粒子@介孔SiO₂复合材料.该纳米复合材料具有超顺磁性,在商用磁铁下可实现快速富集、分离.SiO₂的包覆增强了Fe₃O₄超粒子在近红外光区的吸收,提高了其光热性能;介孔结构的构建提高了近红外光的利用率,进一步提升了纳米复合物的光热性能,且介孔SiO₂的壳层越厚,光热性能越优.细胞实验结果表明,Fe₃O₄超粒子@介孔SiO₂在近红外光照射下具有较高的癌细胞杀伤能力.     

磁性Fe3O4纳米粒子的功能化修饰研究进展

Fe₃O₄纳米粒子因其独特的磁学性能和良好的生物相容性,在生物医药、催化剂、环境治理等领域具有良好的应用前景。然而,磁性Fe₃O₄纳米粒子易团聚、在潮湿的空气中易氧化,制约了Fe₃O₄纳米粒子的深度应用。本文结合课题组在磁性Fe₃O₄纳米粒子应用方面的研究成果,综述了磁性Fe₃O₄纳米粒子的功能化修饰,并讨论了磁性Fe₃O₄复合纳米材料发展面临的机遇和挑战。      

FeOOH明胶复合纳米粒子的制备与表征

采用两步法制备具有弱磁性的FeOOH纳米粒子和FeOOH明胶复合纳米粒子. 透射电镜、扫描电镜、红外光谱、X射线衍射和磁滞回线等测量结果表明: 在一定FeCl₃/Fe₂(SO₄)₃/H₂O摩尔比条件下, 加入十二烷基苯磺酸钠(ABS)溶液, 可制备粒径为30～150 nm弱磁性FeOOH纳米粒子, 其磁性可达6.5×10^－6 emu/g. 弱磁性FeOOH纳米粒子可被明胶包裹. 随着FeCl₃/Fe₂(SO₄)₃摩尔比减小或明胶浓度增加, 纳米球粒径增大, 磁性降低. 随着固化时间增加, FeOOH明胶复合纳米粒子的粒径先增大后减小.     

表面分子印迹磁性纳米粒子的制备及其血红蛋白传感应用

以Fe₃O₄磁性纳米粒子为载体、多巴胺（DA）为功能单体、血红蛋白（Hb）为模板分子,用氯铂酸氧化DA生成聚多巴胺（PDA）,同时氯铂酸还原为铂纳米粒子（PtNPs）,与Hb一起负载于Fe₃O₄纳米粒子表面,洗脱Hb后合成了表面分子印迹磁性纳米粒子（印迹Fe₃O₄/PDA-PtNPs）. 将印迹Fe₃O₄/PDA-PtNPs修饰于磁性玻碳基底表面,制得印迹Fe₃O₄/PDA-PtNPs修饰电极. 实验结果表明,印迹Fe₃O₄/PDA-PtNPs具有良好的水溶性,粒径分布均匀,生成的PtNPs具有良好的导电性和刚性. 用印迹Fe₃O₄/PDA-PtNPs构建的传感器对Hb具有良好的识别性,在0.14 ~ 2.7 μg·mL^-1 Hb浓度范围与交流阻抗变化值呈良好的线性关系,检出限（S/N=3）为0.05 μg·mL^-1.     

Preparation of Functionalized Fe3O4@SiO2 Magnetic Nanoparticles for Monoclonal Antibody Purification

Magnetic Fe₃O₄@SiO₂ nanoparticles with superparamagnetic properties were prepared via a reverse mi-croemulsion method at room temperature. The as-prepared samples were characterized by transmission electron mi-croscopy(TEM), X-ray diffractometry(XRD), and vibrating sample magnetometry(VSM). The Fe₃O₄@SiO₂ nanoparticles were modified by (3-aminopropyl)triethoxysilane(APTES) and subsequently activated by glutaraldehyde(Glu). Protein A was successfully immobilized covalently onto the Glu activated Fe₃O₄@SiO₂ nanoparticles. The adsorption capacity of the nanoparticles was determined on an ultraviolet spectrophotometer(UV) and approximately up to 203 mg/g of protein A could be uniformly immobilized onto the modified Fe₃O₄@SiO₂ magnetic beads. The core-shell of the Fe₃O₄@SiO₂ magnetic beads decorated with protein A showed a good binding capacity for the chime-ric anti-EGFR monoclonal antibody(anti-EGFR mAb). The purity of the anti-EGFR mAb was analyzed by virtue of HPLC. The protein A immobilized affinity beads provided a purity of about 95.4%.     

磁性纳米复合物的制备及对铜离子(Ⅱ)的传感检测

采用化学共沉淀法合成硅包覆的磁性纳米粒子Fe_3O_4@SiO_2,进一步通过六亚甲基二异氰酸酯将吡哆酰肼分子(Pyh)接枝到Fe_3O_4@SiO_2表面,制得功能化的磁性纳米复合物(Fe_3O_4@SiO_2-Pyh)。通过傅里叶变换红外光谱、透射电子显微镜、X射线衍射等技术手段对其结构、形貌和磁性能进行了表征。Fe_3O_4@SiO_2-Pyh粒子具有规则的核壳结构,粒径分布在50~55 nm,壳层厚度约为15 nm。Fe_3O_4@SiO_2-Pyh结构中含有酰腙类活性基团—CO—NH—N=CH—,能与Cu~(2+)形成稳定的配合物,在此基础上采用紫外可见吸收光谱特性建立了测定Cu~(2+)的分析方法,线性范围为3.4×10~(-7)~4.5×10~(-6)mol/L,检出限为1.03×10~(-7)mol/L。此外,利用Fe_3O_4@SiO_2-Pyh良好的磁响应,通过外部磁场能够有效地除去水中过量的铜离子,在环境领域具有潜在的应用价值。     

Magnetically separated and N,S co-doped mesoporous carbon microspheres for the removal of mercury ions

Magnetically separated and N, S co-doped mesoporous carbon microspheres (N/S-MCMs/Fe₃O₄) are fabricated by encapsulating SiO₂ nanoparticles within N, S-containing polymer microspheres which were prepared using resorcinol/formaldehyde as the carbon source and cysteine as the nitrogen and sulfur co-precursors, followed by the carbonization process, silica template removal, and the introduction of Fe₃O₄ into the carbon mesopores. N/S-MCMs/Fe₃O₄ exhibits an enhanced Hg²⁺ adsorption capacity of 74.5 mg/g, and the adsorbent can be conveniently and rapidly separated from wastewater using an externalmagnetic field. This study opens up new opportunities to synthesize welldeveloped, carbon-based materials as an adsorbent for potential applications in the removal of mercury ions from wastewater.     

Aluminum-type superparamagnetic adsorbents: Synthesis and application on fluoride removal

Two effective types of superparamagnetic nano-scale adsorbents of bayerite/SiO₂/Fe₃O₄ have been synthesized via three sequential steps: chemical precipitation of Fe₃O₄, coating of SiO₂ on Fe₃O₄ using acidifying method, and further coating of bayerite (Al(OH)₃) on SiO₂/Fe₃O₄ adopting sol–gel (MASG) or homogeneous precipitation (MAHP) methods. The characteristics of MASG and MAHP were identified using the transmission electron microscopy (TEM) micrograph, scanning electron microscopy with energy dispersive X-ray spectroscopy (SEM/EDX), X-ray powder diffractometer (XRD), and superconducing quantum interference device (SQUID). Removal of fluoride from aqueous solution was examined to evaluate the adsorptive capacity of MASG, MAHP, and commercial activated alumina (CA), and the effects of enclosure of Fe₃O₄ with SiO₂ for SiO₂/Fe₃O₄, MASG, and MAHP particles. Among the adsorbents tested under the same experimental condition, MASG is the most effective adsorbent, of which the adsorption capacities are 38 g/kg (based on adsorbent mass of adsorption in terms of equilibrium constant q_L of Langmuir isotherm), and can compete with CA even at a high pH value. The innovative superparamagnetic adsorbents synthesized in this study possess physicochemical stability at pH range of 6–8 and great potential in the adsorption processes due to not only their high adsorption capacity but also the conveniently magnetic separation which can overcome the difficulty in solid–liquid separation for nano-particles in solutions.     

Application of response surface methodology for optimization of Orange Ⅱ removal by heterogeneous Fenton-like process using Fe_3O_4nanoparticles

In this study, Fe3O4nanoparticles（Fe3O4NPs） were successfully prepared via oxidation–precipitation method and characterized by scanning electron microscopy（SEM）, X-ray diffraction（XRD） and Fourier transform infrared spectroscopy（FT-IR）. The characterization results indicated that Fe3O4 NPs with regular crystal structure and a narrow of diameters had been synthesized successfully and had high purity. A series of experiments were carried out to investigate the degradation of Orange II by the obtained heterogeneous Fe3O4 catalysts in the presence of H2O2. The response surface methodology（RSM） based on Box–Behnken design（BBD） was employed to design and optimize individual and interactive effects of the four main independent parameters（catalyst loading, initial p H, reaction temperature and H2O2concentration） on decolorization efficiency of Orange II. A significant quadratic model（p-value 〈0.0001, R2= 0.9369） was derived using analysis of variance（ANOVA）. Optimum conditions were catalyst loading of 1.5 g/L, initial p H of 2.7, reaction temperature of 42 8C and H2O2 concentration of 22 mmol/L, respectively. The predicted decolorization rate under the optimum conditions as determined by the proposed model was 99.55%. Confirmatory tests were carried out and the decolorization rate of 99.49% was observed under the optimum conditions, which agreed well with the model prediction.     

纳米Fe2O3和Fe3O4的制备及其催化高氯酸铵热分解性能的研究——一个仪器分析综合实验

介绍一个仪器分析综合实验——纳米Fe_2O_3和Fe_3O_4的制备及其催化高氯酸铵热分解性能的研究。采用水热法合成纳米Fe_3O_4,进而煅烧得到纳米Fe_2O_3。使用X射线粉末衍射(XRD)对制得的样品结构进行表征,通过透射电镜(TEM)可以发现其为球形颗粒,粒径在10–20 nm范围内。将制得的纳米Fe_2O_3和纳米Fe_3O_4按不同比例加入高氯酸铵(AP)中,通过对混合物进行热分析(TG-DSC),发现纳米Fe_2O_3和纳米Fe_3O_4可以明显促进AP的分解,且Fe_2O_3的催化效果优于Fe_3O_4的催化效果,并对催化机理进行了简单讨论。通过该实验,可以让学生学习水热反应的方法,掌握利用XRD、热分析等多种手段对化合物结构及性能进行表征的技能。     

磁性反相限进材料的制备及其应用

以磁性二氧化硅（Fe₂O₃@SiO₂）为基质,利用表面原子转移自由基聚合技术（SI-ATRP）,在改性后的Fe₂O₃@SiO₂内表面接枝甲基丙烯酸十八烷基酯（SMA）、外表面接枝甲基丙烯酸缩甘油酯（GMA）,酸解后得到磁性反相限进材料Fe₂O₃@SiO₂-SMA-GMMA,并通过扫描电镜（SEM）、X射线衍射（XRD）、透射电镜（TEM）、振动样品磁强计（VSM）和元素分析对其进行表征。研究表明,Fe₂O₃@SiO₂-SMA-GMMA对牛血清白蛋白（BSA）的排阻能力为90.4%;对磺胺异恶唑（SIZ）、磺胺二甲氧基嘧啶（SDM）、甲氧苄啶（TMP）和磺胺甲基嘧啶（SMR）的最大吸附量分别为2.76、2.24、1.51和1.34 mg/g。Fe₂O₃@SiO₂-SMA-GMMA应用于牛奶和牛血清样品中SIZ、SMR和SDM的分离富集,SIZ、SMR和SDM的加标回收率为88.7%~90.8%,相对标准偏差为3.3%~5.3%。磁性反相限进材料可简化生物基质样品的前处理过程,对血液样品或食品样品等领域的分析检测具有重要价值。     

Assembly of Fe2O3 Nanoparticles with Polymer Microspheres by Encapsulated Copolymerization

Inorganic nanoparticles Fe₂O₃ in diameter of 3-5 nm have been assembled with polymer microspherer and then encapsulated in copolymer of St/BA/AA by polymerization process. Fe₂O₃ encapsulated microspheres can obtained through interaction between surface groups of the two component particles and by adjusting the pH value in the emulsion. Pre-treatment of the composite particles using a certain amount of surfactant before polymerization can give a good encapsulation.     

Fast synthesis of dopamine-coated Fe3O4 nanoparticles through ligand-exchange method

A fast approach was described for the synthesis of water-dispersible monodisperse dopamine-coated Fe₃O₄ nanoparticles（DA-Fe₃O₄） with uniform size and shape via ligand-exchange of oleic acid on Fe₃O₄ using only 2 min.The prepared DA-Fe₃O₄ nanoparticles were characterized by transmission electron microscopy,Fourier transform infrared spectrometry,and vibrating sample magnetometer.The results indicated that the resulting DA-Fe₃O₄ nanoparticles had an average diameter of about 19.2 nm. The magnetic saturation value of the prepared DA-Fe₃O₄ nanoparticles was determined to be 72.87 emu/g,which indicating a well-established superparamagnetic property.