共查询到20条相似文献，搜索用时 11 毫秒
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Mareen Pagel Dr. Rayk Hassert Torsten John Prof. Dr. Manfred Wießler Prof. Dr. Bernd Abel Prof. Dr. Annette G. Beck‐Sickinger 《Angewandte Chemie (International ed. in English)》2016,55(15):4826-4830
Promotion of cell adhesion on biomaterials is crucial for the long‐term success of a titanium implant. Herein a novel concept is highlighted combining very stable and affine titanium surface adhesive properties with specific cell binding moieties in one molecule. A peptide containing l ‐3,4‐dihydroxyphenylalanine was synthesized and affinity to titanium was investigated. Modification with a cyclic RGD peptide and a heparin binding peptide (HBP) was realized by an efficient on‐resin combination of Diels–Alder reaction with inverse electron demand and CuI catalyzed azide–alkyne cycloaddition. The peptide was fluorescently labeled by thiol Michael addition. Conjugating the cyclic RGD and HBP in one peptide gave improved spreading, proliferation, viability, and the formation of well‐developed actin cytoskeleton and focal contacts of osteoblast‐like cells. 相似文献
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Libing Zhang Zongjie Wang Jagotamoy Das Mahmoud Labib Sharif Ahmed Edward H. Sargent Shana O. Kelley 《Angewandte Chemie (International ed. in English)》2019,58(41):14519-14523
In living systems, interfacial molecular interactions control many biological processes. New stimuli‐responsive strategies are desired to provide versatile model systems that can regulate cell behavior in vitro. Described here are potential‐responsive surfaces that control cell adhesion and release as well as stem cell differentiation. Cell adhesion can be modulated dynamically by applying negative and positive potentials to surfaces functionalized with tailored monolayers. This process alters cell morphology and ultimately controls behavior and the fate of the cells. Cells can be detached from the electrode surface as intact clusters with different geometries using electrochemical potentials. Importantly, morphological changes during adhesion guide stem cell differentiation. The higher accessibility of the peptide under a positive applied potential causes phenotypic changes in the cells that are hallmarks of osteogenesis, whereas lower accessibility of the peptide promoted by negative potentials leads to adipogenesis. 相似文献
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Rozkiewicz DI Kraan Y Werten MW de Wolf FA Subramaniam V Ravoo BJ Reinhoudt DN 《Chemistry (Weinheim an der Bergstrasse, Germany)》2006,12(24):6290-6297
We describe a straightforward approach to the covalent immobilization of cytophilic proteins by microcontact printing, which can be used to pattern cells on substrates. Cytophilic proteins are printed in micropatterns on reactive self-assembled monolayers by using imine chemistry. An aldehyde-terminated monolayer on glass or on gold was obtained by the reaction between an amino-terminated monolayer and terephthaldialdehyde. The aldehyde monolayer was employed as a substrate for the direct microcontact printing of bioengineered, collagen-like proteins by using an oxidized poly(dimethylsiloxane) (PDMS) stamp. After immobilization of the proteins into adhesive "islands", the remaining areas were blocked with amino-poly(ethylene glycol), which forms a layer that is resistant to cell adhesion. Human malignant carcinoma (HeLa) cells were seeded and incubated onto the patterned substrate. It was found that these cells adhere to and spread selectively on the protein islands, and avoid the poly(ethylene glycol) (PEG) zones. These findings illustrate the importance of microcontact printing as a method for positioning proteins at surfaces and demonstrate the scope of controlled surface chemistry to direct cell adhesion. 相似文献
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Ng CC Magenau A Ngalim SH Ciampi S Chockalingham M Harper JB Gaus K Gooding JJ 《Angewandte Chemie (International ed. in English)》2012,51(31):7706-7710
Smart surfaces presenting both antifouling molecules with a charged functional group at their distal end, and molecules that are terminated by RGD peptides for cell adhesion, were fabricated and characterized (see picture). By applying potentials of +300 or -300?mV, the surfaces could be dynamically switched to make the peptide accessible or inaccessible to cells. 相似文献
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Couet J Samuel JD Kopyshev A Santer S Biesalski M 《Angewandte Chemie (International ed. in English)》2005,44(21):3297-3301
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Kilian KA Böcking T Gaus K Gooding JJ 《Angewandte Chemie (International ed. in English)》2008,47(14):2697-2699
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Steven E. F. Kleijn Dr. Stanley C. S. Lai Prof. Dr. Marc T. M. Koper Prof. Dr. Patrick R. Unwin 《Angewandte Chemie (International ed. in English)》2014,53(14):3558-3586
Metal nanoparticles (NPs) find widespread application as a result of their unique physical and chemical properties. NPs have generated considerable interest in catalysis and electrocatalysis, where they provide a high surface area to mass ratio and can be tailored to promote particular reaction pathways. The activity of NPs can be analyzed especially well using electrochemistry, which probes interfacial chemistry directly. In this Review, we discuss key issues related to the electrochemistry of NPs. We highlight model studies that demonstrate exceptional control over the NP shape and size, or mass‐transport conditions, which can provide key insights into the behavior of ensembles of NPs. Particular focus is on the challenge of ultimately measuring reactions at individual NPs, and relating the response to their structure, which is leading to imaginative experiments that have an impact on electrochemistry in general as well as broader surface and colloid science. 相似文献
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Kyungtae Kang Gyumin Kang Bong Soo Lee Dr. Insung S. Choi Prof. Dr. Yoonkey Nam Prof. Dr. 《化学:亚洲杂志》2010,5(8):1804-1809
The utilization of non‐biofouling poly(oligo(ethylene glycol) methacrylate) (pOEGMA) films as a background material for the generation of neuronal patterns is reported here. Our previously reported method, which was surface‐initiated, atom transfer radical polymerization of OEGMA, and subsequent activation of terminal hydroxyl groups of pOEGMA with disuccinimidyl carbonate, was employed for the generation of activated pOEGMA films on glass. Poly‐L ‐lysine was then microcontact‐printed onto the activated polymer films, followed by backfilling with poly(ethylene glycol) moieties. E18 hippocampal neurons were cultured on the chemically patterned substrate, and the resulting neuronal networks were analyzed by phase‐contrast microscopy and whole‐cell patch clamp method. The results indicated that the pOEGMA films played an important role in the generation of good‐quality neuronal patterns for up to two weeks without any negative effects to neurons. 相似文献
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Christopher J. Bettinger Dr. Robert Langer Prof. Jeffrey T. Borenstein Dr. 《Angewandte Chemie (International ed. in English)》2009,48(30):5406-5415
The interaction of mammalian cells with nanoscale topography has proven to be an important signaling modality in controlling cell function. Naturally occurring nanotopographic structures within the extracellular matrix present surrounding cells with mechanotransductive cues that influence local migration, cell polarization, and other functions. Synthetically nanofabricated topography can also influence cell morphology, alignment, adhesion, migration, proliferation, and cytoskeleton organization. We review the use of in vitro synthetic cell–nanotopography interactions to control cell behavior and influence complex cellular processes, including stem‐cell differentiation and tissue organization. Future challenges and opportunities in cell–nanotopography engineering are also discussed, including the elucidation of mechanisms and applications in tissue engineering. 相似文献
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Lee JH Wernette DP Yigit MV Liu J Wang Z Lu Y 《Angewandte Chemie (International ed. in English)》2007,46(47):9006-9010
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Moya S Azzaroni O Farhan T Osborne VL Huck WT 《Angewandte Chemie (International ed. in English)》2005,44(29):4578-4581
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Surfaces with “dynamicity” whereby surface properties can be modulated by an external stimulus on user demand have been actively exploited for the past decade. These switchable surfaces with dynamic properties are widely used for a number of applications such as micro/nanoarrays, biomolecule immobilization, basic cell studies, and tissue engineering on a variety of materials. This minireview highlights the dynamic control of surface properties on self‐assembled monolayers and focuses on dynamicity that stems from (bio)chemical conversions achieved by electrical potentials, photoillumination, chemical reagents, enzymes, and pH. 相似文献