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18β-甘草次酸A环官能团化衍生物的合成及抗肿瘤活性 总被引:1,自引:0,他引:1
采用化学方法对18β-甘草次酸A环进行改造, 合成了一系列新型18β-甘草次酸A环官能团化衍生物. 对人白血病细胞株HL-60、K562、肝癌细胞BEL-7404及乳腺癌细胞MDA-MB-231的体外抗肿瘤活性筛选结果表明, 化合物5对4种肿瘤细胞系的抑制活性均强于18β-甘草次酸; 构效关系分析表明, A环内烯键的形成对抑制肿瘤细胞增殖有重要影响; 同时利用Hoechst33258和AO/EB染色, 在荧光显微镜下观察到了细胞凋亡小体的形成. 相似文献
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Masatoshi Tsukahara Naoya Shinzato Yasutomo Tamaki Tomoyuki Namihira Toru Matsui 《Applied biochemistry and biotechnology》2009,158(2):476-482
Monascus pilosus NBRC4520 was selected for functional fermented food inoculation for its high lovastatin and low citrinin production with
a deep-red color. For koji (mold rice) with high lovastatin production, separation of the growth phase and lovastatin production
phase by shifting the temperature from 30 to 23 °C increased lovastatin production by nearly 20 times compared to temperature-constant
cultivation. In addition, citrinin was not produced even in the lovastatin production phase, although the pigment was increased.
With temperature-shift cultivation, 225 μg lovastatin/g dry koji was produced in 14 days without citrinin. 相似文献
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Tai Tien Tran Anfeng Mu Yuka Adachi Yasushi Adachi Shigeru Taketani 《Photochemistry and photobiology》2014,90(5):1136-1143
δ‐Aminolevulinic acid (ALA)‐induced protoporphyrin accumulation is widely used in the treatment of cancer, as photodynamic therapy (PDT). To clarify the mechanisms of ALA uptake by tumor cells, we have examined the ALA‐induced accumulation of protoporphyrin by the treatment of colon cancer DLD‐1 and epithelial cancer HeLa cells with γ‐aminobutyric acid (GABA)‐related compounds. When the cells were treated with GABA, taurine and β‐alanine, the level of protoporphyrin was decreased, suggesting that plasma membrane transporters involved in the transport of neurotransmitters contribute to the uptake of ALA. By transfection with neurotransmitter transporters SLC6A6, SLC6A8 and SLC6A13 cDNA, the ALA‐ and ALA methylester‐dependent accumulation of protoporphyrin markedly increased in HEK293T cells, dependent on an increase in the uptake of ALA. When ALA‐treated cells were exposed to white light, the extent of photodamage increased in SLC6A6‐ and SLC6A13‐expressing cells. Conversely, knockdown of SLC6A6 or SLC6A13 with siRNAs in DLD‐1 and HeLa cells decreased the ALA‐induced accumulation. The expression of SLC6A6 and SLC6A13 was found in some cancer cell lines. Immunohistochemical studies revealed that the presence of these transporters was elevated in colon cancerous cells. These results indicated that neurotransmitter transporters including SLC6A6 and SLC6A13 mediate the uptake of ALA and can play roles in the enhancement of ALA‐induced accumulation of protoporphyrin in cancerous cells. 相似文献
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《Journal of heterocyclic chemistry》2018,55(5):1193-1204
A facile one pot multicomponent synthesis of 1,8‐naphthyridinone derivatives was developed using a mild and reusable phosphotungstic acid catalyst. A 2‐amino picoline, benzaldehyde derivatives and 1,3‐dicarbonyl cyclohexane were used to synthesize 1,8‐naphthyridinones, which was achieved by conventional heating under solvent‐free conditions. All synthesized compounds were characterized by spectral analysis and screened for anticancer activity against A549 lung cancer cells. The results from the cell viability assay showed that the synthesized compounds do have a biological effect at various concentrations on the lung cancer cells. Compounds 4F‐p‐CF3 and 4H‐VAN showed potential as an antiproliferative agent and a dose‐dependent decline in cell viability was observed. 相似文献