薄层色谱法分离二元羧酸

利用薄层色谱法分离了二元羧酸,结果令人满意。     

用薄层色谱法分离芳香醇胺药物对映体

用D－10-樟脑磷酸铵作为手性离子对试剂添加到流动相中,在硅胶GF254薄层板上分离了两种芳香醇胺类药物对映体。     

Separation and Determination of Streptomycin by Ion Exchange-High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - A simple and highly sensitive method is developed for the determination of streptomycin in dosage forms and in biological fluids...     

Separation of Bioactive Prostaglandins and their Metabolites by Reversed-Phase Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - Prostaglandins are biological lipid mediators that control a variety of physiological and pathophysiological processes. They...     

Chiral Separation of S-(+)- and R-(−)-Ibuprofen by Thin-Layer Chromatography. An Improved Analytical Procedure

JPC – Journal of Planar Chromatography – Modern TLC - Chiral separations by gas and liquid chromatography (thin-layer chromatography included) remain an analytical challenge, hence even...     

Separation of Ionic Rhodium and Iridium Species by Electrophoresis on Reversed-Phase Thin-Layer Chromatography Plates

Reversed-phase thin-layer chromatography plates can be effectively used for the separation of metal ions in low concentrations by classical low-voltage electrophoresis. Electropherograms of the ionic composition of hydrochloric and perchloric acid solutions of rhodium(III) and iridium(IV) were obtained. These electropherograms are comparable with the results of capillary electrophoresis. Special features of electrophoresis on reversed-phase plates were noted.     

Recent Developments in Thin-Layer Chromatography - II

Abstract

This review updates the one we did 4 years ago. The emphasis is on technique rather than application. Recent advances in adsorbents, plate shape, developing chambers, gradient elution and quantification methods are reviewed and commented on. Theoretical studies of the basic aspects of TLC processes are also reviewed.     

正相高效液相色谱法分离和测定2—（4—羟基苯氧基）丙酸

采用在硅胶柱（ＺＯＲＢＡＸ ＳＩＬ）上，以正己烷：乙酸＝７０：３０：２．５为流动相及ＵＶ检测器（２５４ｎｍ）的高效液相色谱法分离和测定２－（４－羟基苯氧基）丙酸。在选定的色谱操作条例上，２－（４－羟基苯氧基）丙酸与杂质对苯二酚等之间具有良好的分离效果。该方法具有较好的重现性和准确性。     

Surfactants as Separation Modifiers in Chemical Analyses by Thin-Layer Chromatography: A Review

JPC – Journal of Planar Chromatography – Modern TLC - The works reported in the literature (1994–2015) on the use of surfactants as separation modifiers of organic and inorganic...     

Reversed-Phase Systems for the Separation of Coumarins and Furocoumarins by Thin-Layer and High-Performance Liquid Chromatography

Abstract

The retention behaviour of eleven derivatives of counarin was investigated by reversed-phase (RP) thin-layer and high performance liquid chromatography. The linear relationships between log k'or R_M values and the content of organic modifier in the aqueous mobile phase obtained for wide composition ranges indicate that the plots can be used to determine R_Mw and log k_w values by extrapolation to pure water. The effects of individual substituants on the retention and the correlation between TLC and HPLC data was analysed.     

Determination of Lead and Cadmium by Stripping Voltammetry After Separation from Humic Acid by Thin-Layer Chromatography

Abstract

Interferences resulting from the presence of humic acid (HA) and other surface-active substances in a sample matrix decrease the reliability of Pb and Cd determinations obtained by square-wave anodic stripping voltammetry (SWASV). The implementation of thin-layer chromatography (TLC) prior to SWASV minimizes the effect of these interferents by physically separating Pb and Cd from the sample matrix prior to analysis. The determination of both Pb and Cd was shown to be feasible after TLC separation from laboratory standards spiked with 100 ppm humic acid and from a lake water sample spiked with 1 ppm of Pb and Cd.     

Analysis of PCB's by Thin-Layer Chromatography

Abstract

A screening thin layer chromatography (TLC) technique has been developed that can be done in the field. Quantitative analysis by TLC densitometry can be done in a laboratory (or field van). A densitometer is needed for quantitative work by TLC. An Apple computer may be added to use as a computational device. Semi-quantitative results may be obtained by visual comparisons. Confirmations may be made by silver halide visualization on TLC. Dehalogenation with sodium biphenyl, chlorination with antimony pentachloride, or mass spectral confirmation may be used. Quantitative analysis is possible of the derivatized positive samples.     

薄层色谱法分离测定辣椒红色素中的辣椒红

薄层色谱法分离测定辣椒红色素中的辣椒红丁来欣,陈里（南京理工大学化工学院南京２１００１４）１前言辣椒红色素是从红辣椒中提取的一种天然色素,系多种成分的混合物［１］,其主要成分之一是辣椒红。辣椒红属于类胡萝卜素,是维生素Ａ的前体,对人体健康起重要作用,...     

反相液相色谱法分离2-(4-羟基苯氧基)丙酸酯对映体

合成了三-(4-甲基苯甲酸)纤维素酯(MCTB)手性固定相，用反相高效液相色谱法在该手性固定相上对2-(4-羟基苯氧基)丙酸酯对映体进行拆分．实验结果表明，在三-(4-甲基苯甲酸)纤维素酯手性固定相上，以甲醇与水的体积分数为75：25做流动相能较好的拆分2-(4-羟基苯氧基)丙酸甲酯、乙酯和丁酯对映体，其分离因子分别为1．38、1．49、0．98；同时还发现2-(4-羟基苯氧基)丙酸酯中酯基团的大小对其对映体的分离也有明显的影响，其中以乙酯的拆分效果最佳。     

薄层色谱-反相高效液相色谱法分离纯化蟾酥中的内源性钠泵抑制因子

 采用薄层色谱法分离中药蟾酥中内源性哇巴因样物质 ,以三氯甲烷 甲醇 水 (体积比为 75∶2 0∶5 )溶液为展开剂 ,将所获得的活性物用反相高效液相色谱法纯化并经 2 2 0nm及 30 0nm检测后 ,以人红细胞86Rb摄取率试验测定其生物活性 ,得到了匀质的、紫外吸收波长为 2 5 0nm、可明显抑制钠泵活性的一种新的哇巴因样物质。实验结果为薄层色谱 高效液相色谱法用于内源性、低相对分子质量的生物样品的分离及纯化提供了依据。     

Separation and Determination of Chloroplast Pigments from Spinach by Thin-Layer Chromatography: a Student Laboratory Experiment

JPC – Journal of Planar Chromatography – Modern TLC -     

Characterization of Derivatives of Some Closely Related 1-Picolinoyl-3-methyl-4-(substituted)-pyrazoline-5-ones by Thin-Layer Chromatography

Abstract

The antitubercular activity associated with pyrazoline-5-ones (1), picolinic acid hydrazide (2) and the antibacterial activity of sulphonamides (3) prompted us to undertake the synthesis of 1-picolinoyl-3-methyl-4-(substituted)-pyrazoline-5-ones (see formula) as possible new biological active agents. In view of their medicinal importance, it is thought worth-while to carry out a TLC separation of these compounds.     

Analysis of Bromo-DragonFLY by High-Performance Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC -     

Thin-Layer Chromatography of Metal Ions Complexed with Anils (V). Detection,Separation and Determination

Abstract

p-Diethylaminoanil of phenylglyoxal a bidentate ligand was used for complexation with Hg(II), UO₂(II), Au(III), Pt(IV) Mg(II), Bi(III), Sb(III) and Be(II) ions. The chelates were characterized by their analysis, molar conductance and infrared spectra. T.L.C. detection, separation and determination of these complexes on starch bound silica gel layers was studied Long persisting dark coloure of the complexes rendered the spots self descernible and no locating agent was required. A maximum four complexes could be resolved and identified. Errors in determinations and maximum separation limits were also deduced.     

Anodic Sampling of Titanium by Thin-Layer Chromatography

JPC – Journal of Planar Chromatography – Modern TLC - Titanium as the pure metal or as commercial titanium alloys has been widely used in dentistry. To determine the composition of...