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1.
《Electroanalysis》2004,16(24):2009-2021
The popularity of contactless conductivity detection in capillary electrophoresis has been growing steadily over the last few years. Improvements have been made in the design of the detector in order to facilitate its handling, to allow easy incorporation into available instruments or to achieve higher sensitivity. The understanding of its fundamental working principles has been advanced and the detection approach has also been transferred to lab‐on‐chip devices. The range of applications has been extended greatly from the initial work on small inorganic ions to include organic species and biomolecules. Concurrent determination of cations and anions by dual injection from opposite ends has been demonstrated as well as sample introduction by using flow‐injection systems for easy automation of the process. 相似文献
2.
介绍了电容耦合非接触电导检测(C4D)的检测原理及其最新的研究进展,引用文献50篇。C4D是近几年发展起来的一种用于毛细管电泳和微流控芯片电泳的新检测技术。C4D检测器的原理清楚,结构简单,易于微型化、集成化,不污染检测电极,因而很有应用价值。 相似文献
3.
Simultaneous determination of caffeine,paracetamol, and ibuprofen in pharmaceutical formulations by high‐performance liquid chromatography with UV detection and by capillary electrophoresis with conductivity detection
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Rafael R. Cunha Sandro C. Chaves Michelle M. A. C. Ribeiro Lívia M. F. C. Torres Rodrigo A. A. Muñoz Wallans T. P. Dos Santos Eduardo M. Richter 《Journal of separation science》2015,38(10):1657-1662
Paracetamol, caffeine and ibuprofen are found in over‐the‐counter pharmaceutical formulations. In this work, we propose two new methods for simultaneous determination of paracetamol, caffeine and ibuprofen in pharmaceutical formulations. One method is based on high‐performance liquid chromatography with diode‐array detection and the other on capillary electrophoresis with capacitively coupled contactless conductivity detection. The separation by high‐performance liquid chromatography with diode‐array detection was achieved on a C18 column (250×4.6 mm2, 5 μm) with a gradient mobile phase comprising 20–100% acetonitrile in 40 mmol L?1 phosphate buffer pH 7.0. The separation by capillary electrophoresis with capacitively coupled contactless conductivity detection was achieved on a fused‐silica capillary (40 cm length, 50 μm i.d.) using 10 mmol L?1 3,4‐dimethoxycinnamate and 10 mmol L?1 β‐alanine with pH adjustment to 10.4 with lithium hydroxide as background electrolyte. The determination of all three pharmaceuticals was carried out in 9.6 min by liquid chromatography and in 2.2 min by capillary electrophoresis. Detection limits for caffeine, paracetamol and ibuprofen were 4.4, 0.7, and 3.4 μmol L?1 by liquid chromatography and 39, 32, and 49 μmol L?1 by capillary electrophoresis, respectively. Recovery values for spiked samples were between 92–107% for both proposed methods. 相似文献
4.
《Journal of separation science》2018,41(12):2623-2631
A new method for the rapid determination of the metabolites oxalate and citrate in urine samples was based on capillary electrophoresis and capacitively coupled contactless conductivity detection coupled with solid‐phase extraction. The detection cell for capacitively coupled contactless conductivity detection was improved with a smaller inner volume (1.5 nL), reduced noise (0.2∼0.5 mV) and better reproducibility and durability. Under optimal conditions, oxalate and citrate can achieve baseline separation within 4 min and the detection limits (S/N = 3) for oxalate and citrate are about 44 and 244 ng/mL, respectively. The overall recovery is between 80.0 and 89.2%. This method offers a better choice for quantitative analysis of strong anions such as oxalate and citrate in diagnostic testing associated with human diseases. 相似文献
5.
Simple in‐house flow‐injection capillary electrophoresis with capacitively coupled contactless conductivity method for the determination of colistin
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Patcharin Chaisuwan Thararat Moonta Areeporn Sangcakul Duangjai Nacapricha Prapin Wilairat Kanchana Uraisin 《Journal of separation science》2015,38(6):1035-1041
An in‐house flow‐injection capillary electrophoresis with capacitively coupled contactless conductivity detection method was developed for the direct measurement of colistin in pharmaceutical samples. The flow injection and capillary electrophoresis systems are connected by an acrylic interface. Capillary electrophoresis separation is achieved within 2 min using a background electrolyte solution of 5 mM 2‐morpholinoethanesulfonic acid and 5 mM histidine (pH 6). The flow‐injection section allows for convenient filling of the capillary and sample introduction without the use of a pressure/vacuum manifold. Capacitively coupled contactless conductivity detection is employed since colistin has no chromophore but is cationic at pH 6. Calibration curve is linear from 20 to 150 mg/L, with a correlation coefficient (r2) of 0.997. The limit of quantitation is 20 mg/L. The developed method provides precision, simplicity, and short analysis time. 相似文献
6.
Elimination of the artefact peaks in capillary electrophoresis determination of glutamate by using organic solvents in sample preparation
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Camila Dalben Madeira Campos Patricia Aparecida deCampos Braga Felix Guillermo Reyes Reyes José Alberto Fracassi daSilva 《Journal of separation science》2015,38(21):3781-3787
Focusing on the demand from the food industry for fast and reliable alternative methods to control the quality of food products, we present in this paper a method for amino acid separation and glutamic acid quantification in complex matrices employing capillary electrophoresis with capacitively coupled contactless conductivity detection. We demonstrate by simulation and experimentally the use of organic solvents in sample preparation to prevent peak splitting and increase stacking in capillary electrophoretic separations of amino acids. Additionally, we obtained results for glutamic acid quantification comparable to those obtained via traditional methods used at industrial sites. We tested premium and low‐cost samples with large variations in their glutamic acid content, which demonstrated the wide range of applicability of the method presented herein. The results of the proposed capacitively coupled contactless conductivity detection based capillary electrophoresis method agreed with those obtained by an enzymatic detector and ultra high performance liquid chromatography coupled to tandem mass spectrometry, considering a confidence level of 95%. 相似文献
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8.
《Biomedical chromatography : BMC》2017,31(9)
Capacitively coupled contactless conductivity detection (C4D) has appeared as a powerful technique for the detection of compounds lacking chromogenic or fluorogenic group. Since our last review (Biomedical Chromatography 2014; 28 : 1502–1506) several new capillary electrophoresis (CE)‐C4D methods have been reported. This review provides an update of the most recent utilization of CE‐C4D in the field of pharmaceutical, biomedical and food analysis covering the period from February 2014 to October 2016. The use of CE with C4D in the pharmaceutical field has been shown in many papers. Examples illustrate the applicability of CE‐C4D in the fields of pharmaceutical, biomedical and food analysis. Finally, general conclusions and perspectives are provided. 相似文献
9.
A review of the recent achievements in capacitively coupled contactless conductivity detection 总被引:1,自引:0,他引:1
Capacitively coupled contactless conductivity detection (C4D) in the axial electrode configuration was introduced in 1998 as a quantification method for capillary electrophoresis. Its universality allows the detection of small inorganic ions as well as organic and biochemical species. Due to its robustness, minimal maintenance demands and low cost the popularity of this detector has been steadily growing. Applications have recently also been extended to other analytical methods such as ion chromatography, high-performance liquid chromatography and flow-injection analysis. C4D has also found use for detection on electrophoresis based lab-on-chip devices. Theoretical aspects of C4D in both the capillary and microchip electrophoresis format have been comprehensively investigated. Commercial devices are now available and the method can be considered a mature detection technique. In this article, the achievements in C4D for the time period between September 2004 and August 2007 are reviewed. 相似文献
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11.
Frank-Michael Matysik 《Mikrochimica acta》2008,160(1-2):1-14
Amperometric and conductometric detection are currently the two major electrochemical detection modes in capillary and chip
electrophoresis. The ease of miniaturization and integration of electrochemical detection elements offers a high potential
for the development of portable analytical devices based on electromigrative separations. The challenges and basic concepts
of both detection principles in the context of capillary/chip electrophoresis are shortly introduced and milestones of the
methodical developments are summarized from a historical perspective. Recent advances and applications are discussed with
more detail. Particular attention is paid to new trends in this area of research such as measurements in short capillaries
and the enormous progress and increased popularity of contactless conductivity detection.
Correspondence: Frank-Michael Matysik, Institute of Analytical Chemistry, University of Leipzig, Linnéstr. 3, D-04103 Leipzig,
Germany 相似文献
12.
毛细管电泳(CE)电导检测(CD)是相对较灵敏和仪器结构简单的一项溶液分析技术,尤其是对于无生色团的无机离子分析更具有突出优势,因此,CE-CD技术近年来得到了较快发展[1],并已推出商品化的毛细管电泳电导检测器[2]。但CE和CD的偶联目前还存在如下几个问题:第一,加工适合于毛细管 相似文献
13.
《Journal of separation science》2018,41(14):2969-2975
Ammonium and diphenhydramine are active ingredients commonly found in the same pharmaceutical preparations. We report, for the first time, a sub‐minute method for the simultaneous determination of ammonium and diphenhydramine. The method is based on capillary electrophoresis with capacitively coupled contactless conductivity detection. Both analytes can be quantified in a single run (∼80 injections/h) using 30 mmol/L 2‐(N‐morpholino)ethanesulfonic acid and 15 mmol/L lithium hydroxide (pH 6.0) as background electrolyte. The separation by capillary electrophoresis was achieved on a fused‐silica capillary (50 cm total length, 10 cm effective length, and 50 μm inside diameter). The limits of detection were 0.04 and 0.02 mmol/L for ammonium and diphenhydramine, respectively. The proposed method also provided adequate recovery values for spiked samples (100–106 and 97–104% for ammonium and diphenhydramine, respectively). The results obtained with the new capillary electrophoresis method were compared with those of the high‐performance liquid chromatography method for diphenhydramine and the Kjeldahl method for ammonium and no statistically significant differences were found (95% confidence level). 相似文献
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A dual capacitively coupled contactless conductivity detector for capillary electrophoresis was developed. The two channels are arranged in a bridge configuration so that one of them acts as a reference whose signal is subtracted. This effectively compensates for the baseline conductivity of the separation buffer so that the electronic zero setting is not necessary. Changes in the buffer composition are automatically accounted for, as are temperature drifts. The system is demonstrated for the detection of inorganic model cations in capillary electrophoresis. Besides the use with two separate capillaries, one of which solely serves as reference, it was also found possible to use a single capillary which is looped back through the reference cell. 相似文献
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18.
《Journal of separation science》2017,40(4):940-947
An axial design of a capacitively coupled contactless conductivity detector was tested in combination with fused‐silica capillaries with internal diameters of 10, 15, and 25 μm, which are used for high‐efficiency electrophoretic separation. The transmission of the signal in the detection probe dependent on the specific conductivity of the solution in the capillary in the range 0–278 mS.m−1 has a complex character and a minimum appears on the curve at very low conductivities. The position of the minimum of the calibration dependence gradually shifts with decreasing frequency of the exciting signal from 1.0 to 0.25 MHz toward lower specific conductivity values. The presence of a minimum on the calibration curves is a natural property of the axial design of contactless conductivity detector, demonstrated by solution of the equivalent electrical circuit of the detection probe, and is specifically caused by the use of shielding foil. The behavior of contactless conductivity detector in the vicinity of the minimum was documented for practical separations of amino acids in solutions of 3.2 M acetic acid with addition of 0–50% v/v methanol. 相似文献
19.
Since the introduction of capillary electrophoresis (CE), conductivity detection has been an attractive means of detection. No additional chemical properties are required for detection, and no loss in sensitivity is expected when miniaturising the detector to scale with narrow-bore capillaries or even to the microchip format. Integration of conductivity and CE, however, involves a challenging combination of engineering issues. In conductivity detection the resistance of the solution is most frequently measured in an alternating current (AC) circuit. The influence of capacitors both in series and in parallel with the solution resistance should be minimised during conductivity measurements. For contact conductivity measurements, the positioning and alignment of the detection electrodes is crucial. A contact conductivity detector for CE has been commercially available, but was withdrawn from the market. Microfabrication technology enables integration and precise alignment of electrodes, resulting in the popularity of conductivity detection in microfluidic devices. In contactless conductivity detection, the alignment of the electrodes with respect to the capillary is less crucial. Contactless conductivity detection (CCD) was introduced in capillary CE, and similar electronics have been applied for CCD using planar electrodes in microfluidic devices. A contactless conductivity detector for capillaries has been commercialised recently. In this review, different approaches towards conductivity detection in capillaries and chip-based CE are discussed. In contrast to previous reviews, the focus of the present review is on the technological developments and challenges in conductivity detection in CE. 相似文献
20.
《Electrophoresis》2018,39(19):2425-2430
Determination of natural preservatives using electrophoretic or chromatographic techniques in fermented milk products is a complex task due to the following reasons: (i) the concentrations of the analytes can be below the detection limits, (ii) complex matrix and comigrating/coeluting compounds in the sample can interfere with the analytes of the interest, (iii) low recovery of the analytes, and (iv) the necessity of complex sample preparation. The aim of this study was to apply capillary zone electrophoresis coupled with contactless conductivity detection for the separation and determination of nisin in fermented milk products. In this work, separation and determination of natural preservative–nisin in fermented milk products is described. Optimized conditions using capillary zone electrophoresis coupled with capacitance‐to‐digital technology based contactless conductivity detector and data conditioning, which filter the noise of the electropherogram adaptively to the peak migration time, allowed precise, accurate, sensitive (limit of quantification: 0.02 μg/mL), and most importantly requiring very minute sample preparation, determination of nisin. Sample preparation includes following steps: (i) extraction/dilution and (ii) centrifugation. This method was applied for the determination of nisin in real samples, i.e. fermented milk products. The values of different nisin forms were ranging from 0.056 ± 0.003 μg/mL to 9.307 ± 0.437 μg/g. 相似文献