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We have reported the photochemical regulation of the intracellular antisense effect of antisense probes containing a photo‐responsive artificial nucleic acid, 3‐cyanovinylcarbazole nucleoside (CNVK). Here we focus on the importance of the photocrosslinking rate on the inhibitory effect on gene expression using photocrosslinkable antisense probes (pcASOs). The inhibitory effect of pcASOs on GFP gene expression was dependent on the photocrosslinking rate of 3‐cyanovinylcarbazole with d ‐threoninol (CNVD), CNVK, or psoralen. The ultrafast RNA photocrosslinking induced the formation of a thermally irreversible covalent bond between pcASOs and the target RNA. These ASOs strongly inhibited gene expression only when the photocrosslinking rate was faster than the random walk of branch migration. In addition, pcASOs containing CNVD or CNVK targeted the RNAs with secondary structures. These results indicate the regulatory effect of photocrosslinker and photoirradiation energy using pcASOs on the gene expression level. 相似文献
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Inside Cover: Chimeric RNA Oligonucleotides with Triazole and Phosphate Linkages: Synthesis and RNA Interference (Chem. Asian J. 12/2015)
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Dr. Tomoko Fujino Kanako Kogashi Koudai Okada Dr. Martin Mattarella Takeru Suzuki Dr. Kenichi Yasumoto Prof. Kazuhiro Sogawa Prof. Hiroyuki Isobe 《化学:亚洲杂志》2015,10(12):2542-2542
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Dalila Rocco Dr. Lucinda K. Batchelor Dr. Gabriele Agonigi Simona Braccini Prof. Federica Chiellini Silvia Schoch Dr. Tarita Biver Dr. Tiziana Funaioli Prof. Stefano Zacchini Dr. Lorenzo Biancalana Marina Ruggeri Prof. Guido Pampaloni Prof. Paul J. Dyson Prof. Fabio Marchetti 《Chemistry (Weinheim an der Bergstrasse, Germany)》2019,25(65):14735-14735
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