基于大数值孔径环形光锥照明的超分辨光学 显微成像方法研究

提出和发展了一种基于大数值孔径环形光锥照明的远场超分辨光学显微成像新方法, 采用将发光二极管(LED)面光源、窄带滤光和环形光锥照明相结合的特种照明方式, 实现超分辨显微成像. 建立了大数值孔径环形光锥照明成像的物理模型, 根据标量衍射理论, 在不同环形光锥照明时, 推导出光学显微系统像面衍射斑光强分布的理论计算公式; 通过Matlab求解和绘图, 得到衍射斑光强的分布图样, 从理论上证明这一成像方法可以有效提高光学显微镜的分辨率; 建立了相应的显微成像系统, 通过实验验证了该方法可有效改善显微镜的成像质量, 显著提高分辨率; 在中心波长450 nm、环形光锥数值孔径1.125—1.25时, 实验获得的分辨率至少优于150 nm, 与理论研究结果相符合, 从而证明了这一方法的可行性.     

Trapping single molecules by dielectrophoresis

We have trapped single protein molecules of R-phycoerythrin in an aqueous solution by an alternating electric field. A radio frequency voltage is applied to sharp nanoelectrodes and hence produces a strong electric field gradient. The resulting dielectrophoretic forces attract freely diffusing protein molecules. Trapping takes place at the electrode tips. Switching off the field immediately releases the molecules. The electric field distribution is computed, and from this the dielectrophoretic response of the molecules is calculated using a standard polarization model. The resulting forces are compared to the impact of Brownian motion. Finally, we discuss the experimental observations on the basis of the model calculations.     

Orientational selection of molecules in combined laser and electrostatic fields

It is shown that the combined effects of electrostatic and resonant laser fields on a molecular ensemble can be used to control the orientation of molecules by changing their internal state. In contrast to most other schemes for controlling molecular orientation, the proposed method does not require rotational cooling and can be highly efficient at room temperature.     

Phase imaging with rotating illumination

A modified extended-ptychographical-iterative-engine （ePIE） algorithm is proposed to overcome the dis- advantages of ePIE technique and reduce the influence of stage hysteresis or backlash error. The exit wave of a rotatable ＂screen＂ illuminated by plane wave is used as the illumination on the specimen, and the complex transmission functions of the rotatable object and specimen can be simultaneously reconstructed. Compared with the standard x - y scanning PIE algorithm, the proposed algorithm can completely avoid the influence of stage hysteresis （or backlash error）. The proposed algorithm also has higher convergence s!oeed and better accuracy than the standard PIE algorithm.     

Orientational order in liquid crystals exhibited by some binary mixtures of rod-like and bent-core molecules

We report measurements of the temperature variations of the optical birefringence in the nematic (N) and partial bilayer SmA (SmA_d) phases in 4-n-octyloxy 4^′ -cyanobiphenyl made of rod-like (R) molecules and five mixtures of this compound with 1,3-phenylene bis[4-(3-methylbenzoyloxy)] 4^′ -n- dodecylbiphenyl 4^′ -carboxylate, made of bent-core (BC) molecules. The birefringence decreases with the concentration x of the BC molecules but the macroscopic order parameter initially decreases upto 11mol% of BC molecules and subsequently increases with x . This is attributed to the possible formation of polar clusters of BC molecules. Orientation of BC molecules changes between the N and SmA_d phases and the birefringence data in the two phases imply that the kink angle of the BC molecules is ∼ 90^° rather than ∼ 110^° as obtained from calculations which minimize the energy of the molecule. IR spectroscopic measurements on the mixture with 11mol% of BC molecules have been used to estimate the molecular order parameter S of the R molecules, and to provide additional support for a relatively small kink angle of BC molecules.     

Longitudinal field modes probed by single molecules

We demonstrate that a strong longitudinal, nonpropagating field is generated at the focus of a radially polarized beam mode. This field is localized in space and its energy density exceeds the energy density of the transverse field by more than a factor of 2. Single molecules with fixed absorption dipole moments are used to probe the longitudinal field. Vice versa, it is demonstrated that orientations of single molecules are efficiently mapped out in three dimensions by using a radially polarized beam as the excitation source. We also show that there is no momentum or energy transport associated with the longitudinal field.     

Orientational order and polarizability of molecules in a nematic liquid crystal

The influence of the orientational order of molecules in a nematic liquid crystal on the mean value $\bar \gamma$ and anisotropy Δγ of the molecular polarizability has been studied in the framework of the molecular statistical approach with allowance for the perturbation of the electronic structure of molecules due to the change in the conformation of their aromatic core and intermolecular interactions. Experimental dependences of $\bar \gamma$ and Δγ on the molecular orientational order parameter S have been derived, and their specific features for the known objects have been explained. The possibility of separating the contributions of opposite signs to the dependence Δγ(S) due to the change in the conformation of molecules and intermolecular interactions has been shown using nematic MBBA as an example.     

Three-dimensional particle imaging by defocusing method with an annular aperture

We propose an annular-aperture-based defocusing technique for three-dimensional (3D) particle metrology from a single camera view. This simple configuration has high optical efficiency and the ability to deal with overlapped defocused images. Initial results show that an uncertainty in depth of 23 microm can be achieved over a range of 10 mm for macroscopic systems. This method can also be applied in microscopy for the measurement of fluorescently doped microparticles, thus providing a promising solution for 3D flow metrology at both macroscales and microscales.     

Sensing single electrons with single molecules

We propose a new methodology for probing transport of just one electron, a process of great importance both in nature and in artificial devices. Our idea for locating a single electron is analogues to the conventional GPS where signals from several satellites are used to locate a macro object. Using fluorescent molecules as tiny sensors, it is possible to determine 3D displacement vector of an electron.     

High-resolution imaging of single fluorescent molecules with the optical near-field of a metal tip

We show that a concentration of light at a metal tip allows near-field optical imaging of single fluorescent dye molecules at very high resolution, despite strong quenching effects. Details as small as 10 nm were observed in the fluorescence patterns of single Cy-3 dyes bound to the termini of DNA. Data evaluation by model fitting determines the positions of the dyes to an accuracy even better than 1 nm and also yields their 3D orientation. The metal tip simultaneously provides high-resolution topographic imaging complementing the optical signal for a detailed surface examination.     

Real time measurements of rough object vibration by imaging of Gaussian illumination

A real time method for measuring the vibration of rough surface using image of gaussian illumination is suggested. Theoretical prediction is done by assumption of gaussian distribution of image intensity. Experiments confirming the usefulness of the method have been performed.     

Multiphoton excitation of molecules by single mode CW lasers

An intracavity laser interaction zone with molecules expanded in a molecular beam set-up permits multiphoton excitation under collision-free conditions by narrow band radiation. Two- and three-laser experiments are performed to study rapid adiabatic passage processes, stimulated de-excitation and hole burning effects. The detection of excitation occurs by means of a sensitive bolometric molecular beam detector. The essential information obtained for SF₆ concerns the vibrational bottleneck, the conservation of excited eigenstate characteristics for at least 1 ms, the ease with which about 15 photons can be deposited in the molecules with the help of two cw CO₂ lasers and the influence of simultaneous two-frequency radiation, yielding a significant extra-excitation. Other molecules where multiphoton excitation is observed utilizing the same set-up are CF₃I and CF₃Br.     

全内反射荧光显微术应用于单分子荧光的纵向成像

利用这种显微术中激发场的纵向强度呈指数衰减的分布特性，测得的荧光强 度将强烈依赖渗透深度（强度衰减到1/e时的距离），从而快速直接的确定单分子荧光 团间的纵向间隔、每个荧光团的纵向绝对位置和荧光团的半径大小，即实现荧光分子三维分 布的重构.在整个重构的过程中，只需要改变一次入射角的大小，即只需探测两幅荧光分子 的全内反射成像. 关键词： 全内反射荧光显微术 纵向重构     

Coherent x-ray diffraction imaging with nanofocused illumination

Coherent x-ray diffraction imaging is an x-ray microscopy technique with the potential of reaching spatial resolutions well beyond the diffraction limits of x-ray microscopes based on optics. However, the available coherent dose at modern x-ray sources is limited, setting practical bounds on the spatial resolution of the technique. By focusing the available coherent flux onto the sample, the spatial resolution can be improved for radiation-hard specimens. A small gold particle (size <100 nm) was illuminated with a hard x-ray nanobeam (E=15.25 keV, beam dimensions approximately 100 x 100 nm2) and is reconstructed from its coherent diffraction pattern. A resolution of about 5 nm is achieved in 600 s exposure time.     

Orientational resolvation of S1-excited 4,4'-dinitrophenyl-1,3,5-hexatriene molecules in alcohols

Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 53, No. 6, pp. 961–967, December, 1990.     

Lens-free coherent modulation imaging with collimated illumination

We propose a lens-free coherent modulation imaging(CMI) method for reconstructing a general complex-valued wave field from a single frame of a diffraction pattern. A numerical Fourier transform is introduced in the iterative reconstruction process to replace the lens or zone plate used in the current CMI technique to adopt the constraint on the Fourier components of the exit wave field of the sample. While the complexity of the experimental setup is remarkably reduced by replacing the zone plate and additional accessories with the numerical processing, the energy fluence loss induced by the undesired diffraction orders of the zone plate can be also avoided. The feasibility of the proposed technique is verified experimentally with visible light.     

单分子的光学探测

介绍了三能级结构单分子光子源的工作原理,研究了利用Hanbury-Brown-Twiss探测方法,记录两个单光子计数器响应的单分子光子源输出的每一个事件,分析单事件的光子统计概率P(n, n=0,1,2)与Mandel参数.     

单分子的光学探测

介绍了三能级结构单分子光子源的工作原理,研究了利用Hanbury-Brown-Twiss探测方法,记录两个单光子计数器响应的单分子光子源输出的每一个事件,分析单事件的光子统计概率P（n,n=0,1,2）与Mandel参数.研究结果表明,当信号背景比SBR＞2.41时,P（2）＜（2-√4-2n^-）^2 /2是判别单分子的充分条件,n^-为每个激发周期内探测到的平均光子数。