一种基于全息术的光学系统闭环像差补偿方法

提出了一种基于21单元变形镜与全息波前传感器的全息自适应光学系统, 并对其像差校正能力进行了分析. 首先描述了全息波前传感器基本原理, 并在薄全息图近似下给出基于快速傅里叶变换算法的全息波前传感器数值模型; 然后基于21单元变形镜的数值模型, 分析了该变形镜的波前校正能力; 在此基础上, 数值模拟并实验验证了全息自适应光学系统对静态像差的闭环校正能力.     

无透镜傅里叶变换数字全息术中非共面误差的自动补偿算法

无透镜傅里叶变换数字全息术对活细胞进行显微相衬成像测量时,由于细胞处于运动状态导致记录物体与参考点源难以共面,从而引入成像误差.本文提出了一种有效可靠的基于位相分布的自动对焦再现算法,只需通过一幅全息图,即可确定出记录距离的优化数值解,并结合一次位相线性拟合,成功补偿掉非共面误差.将这种算法应用到活体宫颈癌细胞的相衬成像实验中获得了较为理想的实验结果,表明了这种自动补偿非共面误差算法的可行性. 关键词： 数字全息 无透镜傅里叶变换全息 自动对焦 位相畸变校正     

优化的数字全息显微成像系统

基于预放大数字全息显微系统的全息图记录与再现过程及其点扩散函数的分析, 从成像分辨率、成像质量及实现的难易程度等方面对数字全息显微中六种常见记录光路系统的成像性能进行了对比研究. 结果表明, 像面数字全息术具有最高的成像分辨率及成像质量, 其成像分辨率与记录器件的光敏面尺寸无关, 该系统对信息的记录是完整的, 而且记录过程不必考虑物体被照亮区域的大小, 再现过程非常简单, 是优化的数字全息显微成像系统. 等波面弯曲的物参光像面数字全息术非常有利于位相解包裹及位相畸变补偿的正确进行, 该系统更适合于位相显微. 实验结果验证了理论分析的正确性. 关键词： 显微数字全息 预放大数字全息 像面数字全息 分辨率     

图像传感器像素化效应对菲涅耳非相干关联全息分辨率的影响

作为复光场显微成像的一种新技术,菲涅耳非相干关联全息术(Fresnel incoherent correlation holography,FINCH)因其非相干光记录的特点在近年来受到关注.FINCH作为一种新型非相干全息系统,如何设计光路实现其最佳的分辨率是一个关键问题.然而,针对这个问题的讨论,目前已有文献存在不同的观点,有关FINCH最佳分辨率的成像条件仍有待研究.全息图有效孔径大小是决定全息成像系统分辨率的重要因素,在FINCH系统中,全息记录距离的变化则会引起全息图有效孔径发生变化,全息图的有效孔径大小不仅与光路各元件的孔径有关,还与相干光波相互干涉叠加区域的面积以及图像传感器的像素间距等因素有关.本文基于波动光学理论,结合FINCH全息图的波带结构特征,研究了FINCH全息图的有效孔径.研究发现数字全息记录相机的像素化特性是影响FINCH成像分辨率的决定性因素,并进一步通过数值模拟和光学实验验证了理论分析结果:全息图记录距离(Z_h)等于空间光调制器加载的衍射透镜焦距(f_d)时,FINCH系统的再现像将会达到最佳横向分辨率,且分辨率随成像距离|Z_h-f_d|的增大而降低.     

用于人眼视网膜成像照明的激光消散斑技术研究

以近红外激光(808 nm)作为人眼波前像差探测的信号光和视网膜成像的照明光,液晶空间光调制器(LCOS)作为波前校正器,用哈特曼波前探测器探测人眼像差,构建了人眼像差自适应校正的视网膜成像系统.利用模拟眼分析了激光散斑对相机成像的影响和对哈特曼波前探测器进行像差探测的影响,同时验证了利用旋转散射体的方法消除激光散斑的可行性和有效性;用活体人眼进行了激光消散斑前后照明视网膜进行成像的对比实验,并进一步利用自适应光学技术实现了对人眼像差的动态校正和视网膜细胞的连续成像.校正后,系统波前像差的均方根值小于0.1λ.实验表明激光消散斑后可以同时作为人眼像差探测的信号光和视网膜成像的照明光,从而可以进行连续自适应校正和成像.     

长工作距离显微成像数字全息合成孔径方法(英文)

提出了一种用于长工作距离下显微成像的数字全息合成孔径方法。首先,在不同斜入射照明条件下记录多幅包含不同物光频谱范围的数字全息图。然后,利用每一幅全息图通过数字方法重构并放大物体强度像。最后,通过非相干叠加这些强度像得到分辨力提高且散斑噪声减小的合成物体强度像。理论分析和实验结果表明,此方法可应用于长工作距离下的原位显微观测。     

基于模型的无波前探测自适应光学系统

基于波前梯度的二阶矩和修正后的远场强度分布近似呈线性关系,设计了一种基于模型的无波前探测自适应光学系统快速闭环控制算法。使用61单元变形镜、CCD成像器件等建立了自适应光学系统仿真平台,并以不同湍流强度下的波前像差作为校正对象,分析了这种基于模型的无波前探测自适应光学系统的收敛速度、校正能力及对不同像差的适应性。结果表明,基于模型的无波前探测自适应光学系统在快速收敛的同时,能够获得接近波前校正器件的理想校正能力。N阶模式像差校正时,系统只需要进行N+1次远场光斑的测量。和现有的各种无波前探测自适应光学系统控制算法相比较,基于模型的无波前探测自适应光学系统所需的测量次数大大减少。     

基于滤波成像的大视角数字全息技术

为提高数字全息再现像视角,提出一种基于滤波成像的数字全息技术来实现大视角的三维物体面型测量.利用离轴像面数字全息技术,通过在4F相干图像处理系统的空间频谱面处放置可移动的低通滤波器,使满足CCD分辨率的物光波与参考光波干涉形成全息图,并控制低通滤波及成像区域分别记录不同谱段的子全息图.再现时,首先对子全息图进行数字傅里叶变换,重构对应频谱段,并对频谱段进行拼接形成完整的物光频谱|而后通过数字再现获得大视角的数字全息再现像.利用该方法测量了圆柱形表面（光滑的缝纫针）的三维形貌,并取得了较好的实验结果.     

基于滤波成像的大视角数字全息技术

为提高数字全息再现像视角,提出一种基于滤波成像的数字全息技术来实现大视角的三维物体面型测量.利用离轴像面数字全息技术,通过在4F相干图像处理系统的空间频谱面处放置可移动的低通滤波器,使满足CCD分辨率的物光波与参考光波干涉形成全息图,并控制低通滤波及成像区域分别记录不同谱段的子全息图.再现时,首先对子全息图进行数字傅里叶变换,重构对应频谱段,并对频谱段进行拼接形成完整的物光频谱;而后通过数字再现获得大视角的数字全息再现像.利用该方法测量了圆柱形表面(光滑的缝纫针)的三维形貌,并取得了较好的实验结果.     

基于TPX透镜的连续太赫兹波像面数字全息成像研究

太赫兹波对非极性材料具有良好的穿透性,与太赫兹波强度成像相比,相衬成像能够更好的反映物体的内部结构.本文提出了基于TPX透镜的连续太赫兹波像面数字全息相衬成像方法,研究了全息记录和再现过程,利用曲面拟合畸变校正去除TPX透镜引入的二次相位畸变.实验搭建了太赫兹像面数字全息成像系统,利用振幅型西门子星样品对系统的成像分辨...     

Review of Fresnel incoherent correlation holography with linear and non-linear correlations

Fresnel incoherent correlation holography(FINCH) is a well-established incoherent imaging technique. In FINCH, three selfinterference holograms are recorded with calculated phase differences between the two interfering, differently modulated object waves and projected into a complex hologram. The object is reconstructed without the twin image and bias terms by a numerical Fresnel back propagation of the complex hologram. A modified approach to implement FINCH by a single camera shot by pre-calibrating the system involving recording of the point spread function library and reconstruction by a nonlinear cross correlation has been introduced recently. The expression of the imaging characteristics from the modulation functions in original FINCH and the modified approach by pre-calibration in spatial and polarization multiplexing schemes are reviewed. The study reveals that a reconstructing function completely independent of the function of the phase mask is required for the faithful expression of the characteristics of the modulating function in image reconstruction. In the polarization multiplexing method by non-linear cross correlation, a partial expression was observed, while in the spatial multiplexing method by non-linear cross correlation, the imaging characteristics converged towards a uniform behavior.     

Adaptive optics by incoherent digital holography

Adaptive optics in astronomical and other imaging systems allows compensation of aberrations introduced by random variations of the refractive index in the imaging path. I propose what I believe is a new type of adaptive optics system that dispenses with the hardware lenslet arrays and deformable mirrors of conventional systems. Theoretical and experimental studies show that wavefront sensing and compensation can be achieved by numerical processing of digital holograms of the incoherent object and a guide star. The incoherent digital holographic adaptive optics is seen to be particularly robust and efficient, with envisioned applications in astronomical imaging, as well as fluorescence microscopy and remote sensing.     

基于空间光调制器的非相干数字全息单次曝光研究

菲涅耳非相干相关全息术(Fresnel incoherent correlation holography,FINCH)利用在空间光调制器(spatial light modulator,SLM)上加载双透镜模式对同一物点光分束自相干,并通过改变加载的相位因子得到不同的相移全息图.本系统利用SLM可分区编码调制特性,将FINCH成像中SLM上分三次加载的0°,120°,240°相位双透镜掩模各提取1/3组成一幅复合相移模式加载,并研究了三种相位分布方式对FINCH成像质量的影响.结果表明:三个相位在SLM上分布间隔越大,再现像越清晰.在此基础上,提出了一种新的掩模加载方式,在SLM加载透镜阵列,每一个相位因子对应一个双透镜,具有一个光轴.实验表明,通过这种加载方式,通过SLM后形成的三个相移图能够一次在电荷耦合器上记录,并且三个相移图不重叠,然后通过MATLAB编程计算将不同相移角度的全息图分别提取出来,通过三步相移计算合成一幅包含有物光波的复值全息图,最后通过数值再现算法重建待测样品.此系统可用于对光源相干性较低的实时成像系统,也为微小形变测量、动态物体的观测提供了新方法,为非相干数字全息术的发展提供了新思路.     

基于螺旋相位调制的非相干全息点扩散函数研究

分析了菲涅耳非相干相关全息(Fresnel incoherent correlation holography,FINCH)系统中纯相位空间光调制器(spatial light modulator,SLM)加载螺旋相位掩模时的点扩散函数.以氙灯为照明光源搭建了FINCH系统,电荷耦合器记录的点源全息图与点扩散函数模拟结果一致.采用该系统分别在SLM上加载双透镜掩模和螺旋相位调制双透镜掩模两种情况下对分辨率板和非染色洋葱细胞成像,给出了成像对比结果.结果表明:采用螺旋相位调制的FINCH系统可以在几乎不牺牲分辨率的情况下提高图像的边缘对比度;同样,对相位物体也可以实现图像的边缘提取和识别.该方法在实时监测活细胞的分裂、形变等方面具有重要应用前景.     

Incoherent digital holographic spectral imaging with high accuracy of image pixel registration

Fresnel incoherent correlation holography(FINCH) is a unique three-dimensional(3 D) imaging technique which has the advantages of scanning-free,high resolution,and easy matching with existing mature optical systems.In this article,an incoherent digital holographic spectral imaging method with high accuracy of spectral reconstruction based on liquid crystal tunable filter(LCTF) and FINCH is proposed.Using the programmable characteristics of spatial light modulator(SLM),a series of phase masks,none of whose focal lengths changes with wavelength,is designed and made.For each wavelength of LCTF output,SLM calls three phase masks with different phase constants at the corresponding wavelength,and CCD records three holograms.The spectral images obtained by this method have a constant magnification,which can achieve pixel-level image registration,restrain image registration errors,and improve spectral reconstruction accuracy.The results show that this method can not only obtain the 3 D spatial information and spectral information of the object simultaneously,but also have high accuracy of spectral reconstruction and excellent color reproducibility.     

Phase diversity for calibrating noncommon path aberrations of adaptive optics system under nonideal measurement environment

When the defocus cannot be measured and the wavefront solution set is restricted by a multi-channel, some practical problems exist in the calibration of the noncommon path aberrations of the adaptive optics system. To solve these problems, an evaluation function of phase diversity algorithm is constructed in this paper. We use the method that the estimated aberration and the modulated deformable mirror iterate each other to make up the nonideal measurement environment. Then the ill-pose problem of the solution by phase diversity, produced as relaxing constraints of the diversity defocus on the wavefront solution set, is solved. We have adopted the proposed method to measure the noncommon path aberrations of the adaptive optics system on a 1.23 m telescope. Experimental results demonstrate that wavefront solution is more accurate and the whole imaging quality is improved effectively by using the deformable mirror to compensate the aberration measured.     

基于相位差混合处理方法的高分辨力成像技术

为了提高成像系统的分辨能力, 并尽量减小系统的复杂度, 本文将相位差波前探测技术和相位差图像恢复技术结合起来构成相位差混合处理方法, 给出了点目标和扩展目标情况下混合处理方法的数值仿真结果, 并针对点目标情况进行了实验验证. 实验表明, 在像差较大的情况下, 直接用事后处理方法无法得到满意的结果. 在三种湍流强度下, 经混合方法处理后得到光斑的半高宽分别由自适应光学系统校正后的5.1, 5.1和5.0个像素减小到3.3, 3.2和3.0个像素. 可以看出, 利用相位差混合处理方法得到的图像明显优于单独的事后图像处理方法和自适应光学校正, 相位差混合处理方法在高分辨力成像领域有着巨大的应用潜力.     

Recovering correct phase information in multiwavelength digital holographic microscopy by compensation for chromatic aberrations

We demonstrate experimentally that correct phase imaging without 2pi ambiguity is obtainable in digital holography by using a multiwavelength approach in the microscope configuration. We describe a general approach for removing chromatic aberrations and for controlling the pixel size of the reconstructed phase image in multiwavelength digital holography when the Fourier transform method is adopted for the numerical reconstruction of digital holograms. The retrieved phase is affected by the unavoidable, unwanted chromatic aberration. The correct phase can be obtained by evaluating the phase from the reference holograms reconstructed at different wavelengths to compensate for the chromatic aberration.     

Digital holography as a tool for testing high-aperture micro-optics

The collimation of strongly diverging laser beams emitted by high-power diode lasers is performed with aspherical micro-optical components which have large numerical apertures up to NA=0.7. Conventional interferometric techniques do neither deliver the resolution nor the test frequency and are too costly for an industrial application.Digital holography is presented as a measurement tool for the characterization of micro-optical components in this paper providing a robust and fast technique with a simple and compact setup.The setup presented in this contribution does not apply any imaging or magnifying optics thus avoiding any additional aberrations in the wavefront which would falsify the measurement. The reconstruction process is performed in the computer giving direct access to intensity and phase of the measured wavefront. Additional experimental efforts like, e.g. phase shifting techniques are not needed.The large numerical aperture of the microlenses leads to high fringe densities in the holograms which cannot be resolved by CCD-detectors directly. In order to avoid this problem digital holography is combined with a multiple wavelength technique and a diffusing screen to capture the highly diverging wavefront. An averaging method to reduce the introduced speckle noise is applied and results of the characterization of refractive cylindrical microlenses are presented.     

Selective edge enhancement in three-dimensional vortex imaging with incoherent light

We demonstrate a new imaging method enabling a selective edge contrast enhancement of three-dimensional amplitude objects with spatially incoherent light. The imaging process is achieved in a spiral modification of Fresnel incoherent correlation holography and uses a vortex impulse response function. The correlation recordings of the object are acquired in a one-way interferometer with the wavefront division carried out by a spatial light modulator. Two different methods based on applying a helical reference wave in the hologram recording and a digital spiral phase modulation in image reconstruction are proposed for edge enhancement of amplitude objects. Results of both isotropic and anisotropic spiral imaging are demonstrated in experiments using an LED as an incoherent source of light.