α-石英表面自由基和离体肺泡巨噬细胞内游离Ca2+、Mg2+浓度的关系

本文用ＡＲ－ＣＭ－ＭＩＣ阳离子测定系统及Ｆｕｒａ－２荧光技术，研究了研磨后表面存在自由基的α－石英，和离体肺泡巨噬细胞作用后细胞内游离Ｃａ＾２＾＋浓度（［Ｃａ＾２＾＋）ｉ）和游离Ｍｇ＾２＾＋浓度（［Ｍｇ＾２＾＋］ｉ的关系。发现在含Ｃａ＾２＾＋和Ｍｇ＾２＾＋的介质中，α－石英表面自由基越多，［Ｃａ＾２＾＋］ｉ和［Ｍｇ＾２＾＋］ｉ升高越大；在无Ｃａ＾２＾＋和Ｍｇ＾２＾＋的介质中，新研磨的α－石英能引起     

二氧化硅和硅酸盐对细胞内游离Ca2+和Mg2+浓度的影响

本文研究了不同形态二氧化硅和硅酸盐对细胞内游离Ｃａ＾２＋浓度［Ｃａ＾２＋］ｉ）及游离Ｍｇ＾２＋浓度［Ｍｇ＾２＋］ｉ的影响。结果表明：硅溶胶，聚硅酸，不同硅原子数的固态多酸盐及固态二氧化硅都能使［Ｃａ＾２＋］ｉ和［Ｍｇ＾２＋］ｉ升高。     

Na+/Ca2+交换调节的La3+跨淋巴细胞膜的定量研究

利用荧光浓度指示剂fura-2研究了La³⁺能否利用Na⁺/Ca²⁺交换系统进入人外周血淋巴细胞以及La³⁺Na⁺/Ca²⁺交换的影响. 并首次用此方法研究了La³⁺能否在细胞器(主要为内质网和线粒体)中蓄积. 实验结果表明, 用乌本苷预处理细胞并在无Na⁺介质中测试, 可明显观察到La³⁺跨膜进入淋巴细胞, 而且胞内La³⁺的浓度与胞外的La³⁺浓度成正比. 但当胞外La³⁺浓度大于0.4 mmol/L时, 不再观察到340/380 nm荧光比值的变化, 此时细胞内La³⁺浓度约为1.5×10^-12mol/L. 当La³⁺浓度较大时(0.1 mmol/L)抑制Na⁺/Ca²⁺交换操纵的Ca²⁺的进入, 而较低浓度(0.01 mmol/L)时却刺激Ca²⁺进入. 另外从实验结果可推测La³⁺可以被依赖ATP的质膜钙泵泵出胞外. 胞内钙库用离子霉素耗竭后, La³⁺内流过程中再次加入离子霉素后, 可明显观察到340/380 nm荧光比值增大, 说明La³⁺在细胞器中有一定程度的蓄积. 在模拟胞内离子组分的缓冲液中(pH = 7.05), fura-2对La³⁺的检测限为10^-12mol/L, 对Ca²⁺的检测限为10^-8 mol/L, 并测得fura-2-La³⁺的络合比为1∶1, 表观离解常数为1.7×10^-12 mol/L.     

肺Ca2+含量与硅肺的关系

本文用离子选择电极测定了实验性诱发硅肺大鼠的肺、脑、肾、肝和血清中的[Ca~(2+)]、[K~+]和[Na~+],还以荧光探针Tb~(3+)探讨了Ca~(2+)的作用。在硅肺形成后,大鼠的肺、脑、肾、肝和血清等组织中的游离[Ca~(2+)]均发生变化。荧光探针表明:硅肺大鼠肺Ca~(2+)增加部分来自线粒体Ca~(2+)的释放硅肺。大鼠肺中游离Ca~(2+)和线粒体Ca~(2+)的变化可能与巨噬细胞的损伤有关。     

希土化合物对离体巨噬细胞的影响

本文应用细胞培养法和单细胞阳离子测定系统研究了希土化合物以地巨噬细胞的影响，结果表明，在培养介质中ＳｍＣｌ３和Ｙｃｌ３的浓度大于１ｍｍｏｌ．ｄｍ＾－３时，有明显的细胞毒性，Ｙｃｌ３的细胞毒性大于ＳｍＣｌ３，ＳｍＣｌ３和ＹＣｌ３的细胞毒性明显大于Ｓｍ（Ａｌａ）３Ｃｌ３和Ｙ（Ａｌａ）２Ｃｌ３。希土化合物的作用使细胞（Ｃａ＾２＋ｉ）升高；毒性越大，Ｃａ＾２＋ｉ升高越甚。低浓度Ｓｍ＾３＋和Ｙ６３＋对细胞膜     

粉防己碱拮抗α-石英细胞毒性的机理

借助于单细胞阳离子测定系统研究了α-石英诱发细胞毒性过程中,不同外钙浓度时粉防己碱作用后,细胞存活率与胞浆游离钙浓度的变化关系。研究发现粉防己碱可以通过阻断细胞离子通道,拮抗α-石英的细胞毒性。     

呼吸尘埃中游离晶态α－石英的X射线衍射外标法测定

本实验用Ｘ射线衍射外标法测定了极微量（０．１～５ｍｇ）呼吸尘埃中游离晶态α－ＳｉＯ＿２的含量。其精确度（相对偏差）小于１０％，达到常规微量（５～１０ｍｇ）样品的分析精确度。     

α－石英表面自由基和离体肺泡巨噬细胞内游离Ca~（2＋）、Mg~（2＋）浓度的关系

本文用ＡＲ－ＣＭ－ＭＩＣ阳离子测定系统及Ｆｕｒａ－２荧光技术，研究了研磨后表面存在自由基的α－石英，和离体肺泡巨噬细胞作用后细胞内游离Ｃａ２＋浓度（［Ｃａ２＋］）和游离Ｍｇ２＋浓度（［Ｍｇ２＋］ｉ）的关系．发现在含Ｃａ２＋和Ｍｇ２＋的介质中，α－石英表面自由基越多，［Ｃａ２＋］ｉ和［Ｍｇ２＋］ｉ升高越大；在无Ｃａ２＋和Ｍｇ２＋的介质中，新研磨的α－石英能引起［Ｃａ２＋］ｉ和［Ｍｇ２＋］ｉ升高．Ｃａ２＋和Ｍｇ２＋的升高可能部分由于细胞内结合钙和结合镁的释放．     

REBO3中Ce3+和Bi3+对Sm3+光致发光的影响

在紫外光(UV)激发下,系统地研究了REBO_3(RE=La,Gd,Y)中Sm~(3+),Ce~(3+)和Bi~(3+)的发射光谱、激发光谱及其发光强度与组成、结构的关系.结果表明:Ce~(3+)、Bi~(3+)均可敏化LaBO_3中Sm~(3+)的发光;而在GdBO_3和YBO_3中,只有Bi~(3+)能敏化Sm~(3+)的发光,Ce~(3+)猝灭Sm~(3+)的发光.本文还探讨了三种基质中Sm~(3+)发光浓度猝灭的机理.     

新型Ca2+探针用于5-HT诱导细胞钙动员的机制

采用激光共聚焦技术分别进行了新型Ca²⁺荧光试剂STDIn与Fluo-3对胃底平滑肌细胞的标记及其对胞内游离Ca²⁺浓度变化的响应等特性的研究. 结果表明, 与Fluo-3不同, 新型Ca²⁺荧光试剂STDIn对胞浆Ca²⁺具有靶向性, 是一种真正的胞浆Ca²⁺荧光探针, 对5-羟色胺(5-HT)诱导的胞内游离Ca²⁺浓度变化的响应更灵敏迅速. 应用STDIn对5-HT诱导胃底平滑肌细胞内游离Ca²⁺动员的机制研究发现, L型钙通道拮抗剂Mn9202对5-HT升高[Ca²⁺ ]_i有明显的抑制作用; 使用蛋白激酶(protein kinase C, PKC)抑制剂D-Sphingosine孵育后, 再加入5-HT胞内荧光强度明显升高, 而以磷脂酶(phospholipase C, PLC)抑制剂Compound48/80孵育后, 5-HT则不再引起荧光强度的变化. 表明5-HT通过促进外钙内流和内钙释放使[Ca²⁺ ]_i升高; 在胃底平滑肌5-HT升高[Ca²⁺]_i是通过5-HT₂受体介导的IP₃/Ca²⁺和DG/PKC双信使途径; 5-HT通过L型钙通道促进外钙内流; Mn9202亦能通过拮抗5-HT受体而起到抑制5-HT促胞内钙释放的作用.     

PTHALOCYANINE-INDUCED PHOTODYNAMIC CHANGES OF CYTOPLASMIC FREE CALCIUM IN CHINESE HAMSTER CELLS

Exposure to light of Chinese hamster cells preloaded with chloroaluminum phthalocyanine causes an immediate increase of cytoplasmic free calcium, [Ca2+], from about 0.2 microM to 1 microM within 5 min after illumination. This increase was dose-dependent within the biological dose range, reaching a plateau at a dose that kills 99.5% of the cells. Fluoride addition prior to light exposure protected against cell killing and reduced the increase of [Ca2+]i. These findings raise the possibility that changes in [Ca2+]i after photodynamic treatment may be relevant to cell killing and/or other biological responses of the cells, e.g. release of eicosanoids.     

苯甲醛及其衍生物对铈离子存在下丙烯酰胺聚合的影响

<正> 虽然Santappa等早已报道Ce~(4+)(高氯酸铈)/甲醛体系能引发丙烯腈(AN)、甲基丙烯酸甲酯(MMA)的聚合,但对醛的活性以及参与引发单体聚合的自由基都未有详细报道,近来孙燕慧等发现脂肪醛能显著促进Ce~(4+)(硝酸铈铵CAN)引发丙烯酰胺(AAM)的聚合,而且醛的活性远大于相应的醇。至于芳香醛对于Ce~(4+)引发烯类单体的聚合的影响也未见详细报道。本文扼要报道苯甲醛(BA)及其衍生物对-硝基苯甲醛(PNBA)、间-硝基苯甲醛(MNBA)、间-溴苯甲醛(MBBA)、对-氯苯甲醛(PCBA)对Ce~(4+)(CAN)存在下丙烯酰胺聚合的影响,测定了其聚合速率,以UV吸收光谱、ESR波谱测定     

牛磺胆酸钠对胆红素与钙离子相互作用形成沉淀过程的影响的研究

本工作选择牛磺胆酸钠作为人体内胆汁酸盐的代表研究其对胆色素结石的重要成分——胆红素钙沉淀过程的影响。结果表明,牛磺胆酸钠的存在使胆红素钙沉淀的条件溶度积大大提高,并使沉淀反应速度大大减慢。在牛磺胆酸钠浓度较高的情况下,沉淀过程出现诱导期,说明牛磺胆酸钠在一定条件下可以抑制胆红素钙沉淀的形成。沉淀的红外光谱表明,牛磺胆酸并未结合在沉淀中,也未影响沉淀中胆红素与钙离子间的相互作用的部位。沉淀的扫描电镜观察结果经统计学处理表明牛磺胆酸钠的存在使沉淀的颗粒变小,颗粒的聚集程度减小。     

STUDY ON THE CONCENTRATION EFFECTS IN GPC IV A NEW METHOD FOR DETERMINATION OF SECOND VIRIAL COEFFICIENTS FROM THE CONCENTRATION EFFECTS

In this paper a new relation between the second virial coefficients A_2, (?)_w and (dV_(es)/dC)_c→0=K_s was derived from proposed model theory of concentration effects in GPC for mono-and poly-dispersed polymers. Based on this relation a new method for determination of second vifial coefficients from the combination of (dV_(es)/dC)_c→0=K_3, (?)_w and K_H measurements was proposed.The values of A_2 for mono-and poly-dispersed polystyrenes with molecular weight range from 10~4 to 10~6 in good and theta solvents were determined by proposed method. Results show that their values of A_2 are in agreement with those obtained by light scattering.     

胆汁酸盐对胆红素钙沉淀过程的影响

研究了六种人胆汁主要胆汁酸盐对胆红素钙沉淀形成过程的影响。用曲线拟合法处理数据取得热力学及动力学参数。结果表明,六种胆汁酸盐都表现动力学和热力学抑制,而且抑制作用有浓度依赖关系。在一定条件下出现诱导期。虽然不同胆汁酸盐的作用结果相似,但在相同条件下,二羟基胆汁酸和三羟基胆汁酸影响程度不同。     

溴离子对BZ非催化振荡反应的影响

Br~-对Belousov-Zhabotinsky(BZ)振荡反应起着控制作用。近来发现,一些有机物在被BrO_3~-氧化时,即使没有金属离子Ce~(3+),Mn~(2+),Fe(phen)_3~(2+),也可产生振荡反应,称为非催化BZ振荡反应。本文选择酪氨酸(Tyr)-BrO_3~--H_2SO_4 BZ振荡体系,对Br~-的影响作了系统的研究,对机理作了初步探索。     

KINETIC STUDIES ON CHEMILUMINESCENCE OF CHLOROPLASTS INDUCED BY CHANGES IN ION CONCENTRATION

Abstract— Acid-base, acid-, and salt-induced chemiluminescences of chloroplasts were investigated in relation to their dependences upon several time parameters:

• 1 The dependence of the three chemiluminescences on the preillumination time was similar; the luminescences rose to a maximum and then decayed to a steady-state This behaviour depends on the preillumination level and the length of the dark time following the preillumination. Analysis of the above indicates the formation of quenching entities during the preillumination which react with the luminescence precursors mainly in the dark. In contrast, the delayed light measured after 22 msec from the preillumination, showed a much slower but smooth rise to the steady-state with no subsequent drop.
• 2 During the dark period following preillumination the ability of the system to chemi-luminescence decayed with a first order rate.
• 3 During emission the above chemiluminescences decayed with a first order law. The total emission in different experiments was also proportional to the maximum emission, in agreement with a first order law of decay.
• 4 The comparison of the kinetic behaviour of the three types of chemiluminescence indicates common precursors of luminescence for at least the acid-base and the salt types. The delayed emission at 22 msec however, seems to have separate precursors than the three chemiluminescences.

     

A STUDY ON THE ADSORPTION OF LOW CONCENTRATION FREE ALKALI BY SUBACID RESIN

1. INTRODUCTIONIn the purification process of food, drug, and biological products, usually the material to be purified were not neutral. Application of traditional neutralization method with acid or alkali could increase electrolyte content in the material to be purified. Due to the capability of the adsorption of free alkali by subacid resin, the defect of electrolyte absorption in neutralization could be avoided when used in the purification of food, drug, and biological products [1]. H…