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Jeremias Sibold Katharina Kettelhoit Loan Vuong Fangyuan Liu Daniel B. Werz Claudia Steinem 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(49):17969-17977
The receptor lipid Gb3 is responsible for the specific internalization of Shiga toxin (STx) into cells. The head group of Gb3 defines the specificity of STx binding, and the backbone with different fatty acids is expected to influence its localization within membranes impacting membrane organization and protein internalization. To investigate this influence, a set of Gb3 glycosphingolipids labeled with a BODIPY fluorophore attached to the head group was synthesized. C24 fatty acids, saturated, unsaturated, α‐hydroxylated derivatives, and a combination thereof, were attached to the sphingosine backbone. The synthetic Gb3 glycosphingolipids were reconstituted into coexisting liquid‐ordered (lo)/liquid‐disordered (ld) giant unilamellar vesicles (GUVs), and STx binding was verified by fluorescence microscopy. Gb3 with the C24:0 fatty acid partitioned mostly in the lo phase, while the unsaturated C24:1 fatty acid distributes more into the ld phase. The α‐hydroxylation does not influence its partitioning. 相似文献
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Haw‐Young Kwon Xiao Liu Eun Gyeong Choi Jung Yeol Lee So‐Young Choi Jun‐Young Kim Lu Wang Sung‐Jin Park Beomsue Kim Yong‐An Lee Jong‐Jin Kim Nam Young Kang Young‐Tae Chang 《Angewandte Chemie (Weinheim an der Bergstrasse, Germany)》2019,131(25):8514-8519
The rapid and sensitive classification of bacteria is the first step of bacterial community research and the treatment of infection. Herein, a fluorescent probe BacGO is presented, which shows the best universal selectivity for Gram‐positive bacteria among known probes with a minimum staining procedure for sample detection and enrichment of the live bacteria. BacGO could also be used to assess of the Gram status in the bacterial community from wastewater sludge. Furthermore, BacGO could sensitively and selectively detect a Gram‐positive bacterial infection, not only in vitro but also using an in vivo keratitis mouse model. BacGO provides an unprecedented research tool for the study of dynamic bacterial communities and for clinical application. 相似文献
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