L—PROLINE—FUNCTIONALIZED POLYSTYRENE WITH 1—METHYLDECYLENE SPACER AS A CHIRAL STATIONARY PHASE FOR LIGAND—EXCHANGE CHROMATOGRAPHIC RESOLUTION OF AMINO ACID RECEMATES

The L-proline-functionalized polystyrene with 1-methyldecylene spacer was synthesized from 2% divinylbenzene-crosslinked polystyrene gel via 10-bromo-1-methyldecyl polystyrene intermediate.After complexed with copper(Ⅱ） ion,the polymer with L-proline ligand was used as the chiral stationary phase (CSP) for ligand exchange chromatography of amino acid racemates.The results showed that the CSP possessed powerful enantioselectivity and all racemates of the fifteen tried amino acids were completely separated.     

LIGAND EXCHANGE RESIN FOR AMINO ACID RACEMATES

ＬＩＧＡＮＤＥＸＣＨＡＮＧＥＲＥＳＩＮＦＯＲＡＭＩＮＯＡＣＩＤＲＡＣＥＭＡＴＥＳＹｕａｎＺｈｉ；ＨｅＢｉｎｇｌｉｎ（ＩｎｓｔｉｔｕｔｅｏｆＰｏｌｙｍｅｒＣｈｅｍｉｓｔｒｙ，ＮａｎｋａｉＵｎｉｖｅｒｓｉｔｙ，Ｔｉａｎｊｉｎ３０００７１）Ａｂｓｔｒａｃｔ...     

RESOLUTION OF DL－α－AMINO ACIDS ON A L－HYDROXY PROLINE CHIRAL PHASE BY LIGAND－EXCHANGE CHROMATOGRAPHY

ＲＥＳＯＬＵＴＩＯＮＯＦＤＬ－α－ＡＭＩＮＯＡＣＩＤＳＯＮＡＬ－ＨＹＤＲＯＸＹＰＲＯＬＩＮＥＣＨＩＲＡＬＰＨＡＳＥＢＹＬＩＧＡＮＤ－ＥＸＣＨＡＮＧＥＣＨＲＯＭＡＴＯＧＲＡＰＨＹ￥ＴａｏＣＨＥＮ；ＴｉａｎＢａｏＨＵＡＮＧ（ＣｈｅｎｇｄｕＩｎｓｔｉｔｕｔ...     

INTERACTION OF AMINO ACID WITH ION EXCHANGE RESIN Ⅲ.FURTHER INVESTIGA TION OF SUPEREQUIVALENT ADSORPTION MECHANISM OF AMINO ACID ON ION EXCHANGE RESIN

The adsorption isotherms of glycine,alanine and oxidized glutathion on strong acid cation and strong base anion exchange resins from aqueous solutions were measured and the superequivalent adsorptions of glycine and alanine observed.The infrared spectra of glycine adsorbed on the cation and the anion exchange resins,001×7 and 201×7,were measured.From these results,it is concluded that the amino acid adsorption on the ion exchange resin proceeds not only through ion exchange and proton transfer mechanisms,but also through aminecarboxylate interaction between the adsorbed amino acid molecules,and the formation of second layer of amino acid molecules is the mechanism of superequivalent adsorption of amino acid,the carboxylate or amine groups of the first layer of amino acid molecules on the ion exchange resin act as the exchange sites for the second layer of amino acid molecules.     

A NOVEL DERIVATIVE OF β-CYCLODEXTRIN AS CHIRAL SIATIONARY PHASE IN CAPILLARY GAS CHROMATOGRAPHY

A novel β-cyclodextrin derivative, 2, 6-di-O-butyl-3-O-acetyl-β-cyclodex-trin, was prepared and used as chiral stationary phase for capillary gas chromato-graphic resolution of enantiomers, lhe glass capillary columns with the derivativehaving low melting point and high thermal stability could produce more than 3000plates per column meter and have proven to be good chlral columns to separate theenantiomers of some amino acids, derivatives of epoxy ethane and alkyl halide, etc.     

OPTICALLY ACTIVE AMINO ACIDS WITH HIGHLY BRANCHED SIDE CHAIN（Ⅱ）：SYNTHESIS AND RESOLUTION OF RACEMIC 2－ACETAMIDO－7，7－DIMETHYLOCTANOIC ACID AND 2－ACETAMIDO－8，8－DIMETHYLNONANOIC ACID ETHYL ESTERS

ＯＰＴＩＣＡＬＬＹＡＣＴＩＶＥＡＭＩＮＯＡＣＩＤＳＷＩＴＨＨＩＧＨＬＹＢＲＡＮＣＨＥＤＳＩＤＥＣＨＡＩＮ（Ⅱ）：ＳＹＮＴＨＥＳＩＳＡＮＤＲＥＳＯＬＵＴＩＯＮＯＦＲＡＣＥＭＩＣ２－ＡＣＥＴＡＭＩＤＯ－７，７－ＤＩＭＥＴＨＹＬＯＣＴＡＮＯＩＣＡＣＩＤＡＮ...     

THE SYNTHESIS OF ARYLOXY－CARBOXYLIC ACID UNDER MICROWAVE，SOLID BASE AS A SUPPORT

ＴＨＥＳＹＮＴＨＥＳＩＳＯＦＡＲＹＬＯＸＹ－ＣＡＲＢＯＸＹＬＩＣＡＣＩＤＵＮＤＥＲＭＩＣＲＯＷＡＶＥ，ＳＯＬＩＤＢＡＳＥＡＳＡＳＵＰＰＯＲＴＺｈｏｎｇＹｉＷＡＮＧ；ＨａｉＪｉａｎＳＨＩ；ＨａｏＸｉｎＳＨＩ（ＤｅｐａｒｔｍｅｎｔｏｆＣｈｅｍｉｓｔｒｙ．...     

RAPID DETERMINA TION OF L—GLUTAMIC ACID WITH AN ENZYME REACTOR OFL—GLUTAMIC DECARBOXYLASE IMMOBILIZED ON ION EXCHANGE RESIN

The preparation and characterization of an immobilized L-glutamic decarboxylase(GDC) were studied.This work is to develop a sensitive method for the determination of L-glutamate using a new biosensor,which consists of an enzyme column reactor of GDC immobilized on a novel ion exchange resin(carboxymethyl-copolymer of allyl dextran and N.N‘-methylene-bisacrylamide CM-CADB) and ion analyzer coupled with a CO2 electrode.The conditions for the enzyme immobilization were optimized by the parameters:buffer composition and concentration,adsorption equilibration time,amount of enzyme,temperature,ionic strength and pH.The properties of the immobilized enzyme on CM-CADB were studied by investigating the initial ate of the enzyme reaction,the effect of various parameters on the immobilized GDC activity and its stability.An immobilized GDC enzyme column reactor matched with a flow injection system-ion analyzer coupled with CO2 electrode-data collection system made up the original form of the apparatus of biosensor for determining of L-glutamate acid.The limit of detection is 1.0×10^-5M.The linearity response is in the range of 5×10^-2-5×10^-5M.The equation of linear regression of the calibration curve is y=43.3x+181.6(y is the milli-volt of electrical potential response,x is the logarithm of the concentration of the substrate of L-glutamate acid).The correlation coefficient equals 0.99.The coefficient of varioation equals 2.7%.     

A NOVEL IN－SITU SPECTROELECTROCHEMICAL TECHNIQUE WITH PROBE BEAM DEFLECTION：FOR STUDY OF THE ION EXCHANGE BETWEEN FILMS ON ELECTRODE SURFACE AND SOLUTIONS

ＡＮＯＶＥＬＩＮ－ＳＩＴＵＳＰＥＣＴＲＯＥＬＥＣＴＲＯＣＨＥＭＩＣＡＬＴＥＣＨＮＩＱＵＥＷＩＴＨＰＲＯＢＥＢＥＡＭＤＥＦＬＥＣＴＩＯＮ：ＦＯＲＳＴＵＤＹＯＦＴＨＥＩＯＮＥＸＣＨＡＮＧＥＢＥＴＷＥＥＮＦＩＬＭＳＯＮＥＬＥＣＴＲＯＤＥＳＵＲＦＡＣＥＡＮＤ...