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1.
Absolute quantification of genetically modified MON810 maize (Zea mays L.) by digital polymerase chain reaction 总被引:3,自引:0,他引:3
Philippe Corbisier Somanath Bhat Lina Partis Vicki Rui Dan Xie Kerry R. Emslie 《Analytical and bioanalytical chemistry》2010,396(6):2143-2150
Quantitative analysis of genetically modified (GM) foods requires estimation of the amount of the transgenic event relative
to an endogenous gene. Regulatory authorities in the European Union (EU) have defined the labelling threshold for GM food
on the copy number ratio between the transgenic event and an endogenous gene. Real-time polymerase chain reaction (PCR) is
currently being used for quantification of GM organisms (GMOs). Limitations in real-time PCR applications to detect very low
number of DNA targets has led to new developments such as the digital PCR (dPCR) which allows accurate measurement of DNA
copies without the need for a reference calibrator. In this paper, the amount of maize MON810 and hmg copies present in a DNA extract from seed powders certified for their mass content and for their copy number ratio was measured
by dPCR. The ratio of these absolute copy numbers determined by dPCR was found to be identical to the ratios measured by real-time
quantitative PCR (qPCR) using a plasmid DNA calibrator. These results indicate that both methods could be applied to determine
the copy number ratio in MON810. The reported values were in agreement with estimations from a model elaborated to convert
mass fractions into copy number fractions in MON810 varieties. This model was challenged on two MON810 varieties used for
the production of MON810 certified reference materials (CRMs) which differ in the parental origin of the introduced GM trait.
We conclude that dPCR has a high metrological quality and can be used for certifying GM CRMs in terms of DNA copy number ratio. 相似文献
2.
3.
Naz Mohammed Aghatabay Yaghub Mahmiani Hüseyin Çevik Fahrettin Gucin Basaran Dulger 《Structural chemistry》2008,19(5):833-842
Series of new mixed aza-oxo-thia macrocyclic ligands {2,6,12,16-tetraaza-1,7,11,17-tetraoxo-9,19-dithia-[(4′-methyl-5′,4,3′)(14′-methyl-15′,14,13′)]ditriazine}cyclocosane
(L
1
); {2,6,13,17-teraaza-1,7,12,18-tetraoxo-9,10,20,21-tetrathia-[(4′-methyl-5′,4,3′)(15′-methyl-14′,16′,15)]di-triazine}cyclodocosane
(L
2
); {2,6,14,18-tetraaza-1,7,13,19-tetraoxo-10,22-dithia-[(4′-methyl-5′,3′,4)(16′-methyl-15′,17′,16)]ditriazine}cyclotetracosane
(L
3
) and {2,6,15,19-tetraaza-1,7,14,12-tetraoxo-10,11,23,24-tetrathia-[(4′-methyl-5′,4,3′)(17′-methyl-8′,17,16′)]ditriazine}cyclohexa-cosane
(L
4
) were synthesized. The structural features of the compounds have been studied by elemental analyses, Mass, FT-Raman, FT-IR,
1H and 13C NMR spectroscopy. The antimicrobial activities of the ligands were evaluated using disk diffusion method in dimethyl sulfoxide
as well as the minimal inhibitory concentration (MIC) dilution method, against 9 bacteria. The obtained results from disk
diffusion method were assessed in side-by-side comparison with those of Penicillin-g, Ampicillin, Cefotaxime, Vancomycin,
Oflaxacin, and Tetracyclin, well-known antibacterial agents. The results from dilution procedure were compared with Gentamycin
as antibacterial and Nystatin as antifungal. The antifungal activities are reported on five yeast cultures namely Candida albicans, Kluyveromyces fragilis, Rhodotorula rubra, Debaryomyces hansenii, and Hanseniaspora guilliermondii, and the results are referenced with Nystatin, Ketaconazole, and Clotrimazole, commercial antifungal agents. In most cases,
the compounds show strong antifungal activity in the comparison tests. 相似文献
4.
Lutz Preu Wolfgang Kliegel Steven J. Rettig James Trotter 《Monatshefte für Chemie / Chemical Monthly》2001,132(10):1213-1224
Summary. The structure of the dehydrogenation product 1′,3a′-dihydro-3′-((1,3-dioxoindan-2-ylidene)-phenyl-methyl)-5′-phenyl-spiro-(indan-2,1′-pyrrolo[3,4-c]pyrrole)-1,3,4′,6′-(5′H, 6a′H)-tetrone derived from the cycloadducts (±)-(3a′S,6a′R)-1′,3a′-dihydro-3′-((R)-α-(1,3-dioxoindanyl)-benzyl)-5′-phenyl-spiro-(indan-2,1′-pyrrolo[3,4-c]pyrrole)-1,3,4′,6′(5H,6a′H)-tetrone and/or (±)-(3a′S,6a′R)-1′,3a′-dihydro-3′-((S)-α-(1,3-dioxoindanyl)-benzyl)-5′-phenyl-spiro-(indan-2,1′-pyrrolo[3,4-c]pyrrole)-1,3,4′,6′(5H,6a′H)-tetrone, which were synthesized by 1,3-dipolar cycloaddition of N-phenylmaleimide to 2-((2-(1,3-dioxoindan-2-yl)-2-phenyl-ethenyl)-imino)-indan-1,3-dione,
was determined by X-ray analysis. Crystal data (CCD, 180 K): rhombohedral, R&3macr;;, a = 34.0871(7), c = 13.9358(5) ?, Z = 18; the structure was solved by direct methods and refined by full-matrix least-squares procedures to R(F, I ≥ 3σ(I)) = 0.053. The molecule contains a central folded ring system of two cis-fused 5-membered heterocyclic rings; each ring is nearly planar, and the angle between the rings amounts to 59.0°. Dynamic
1H NMR spectroscopy of the product revealed an exchange process caused by restricted rotation of the double bonded 1,3-indandione
moiety and the phenyl group about the Csp2-Csp2 single-bonds. Molecular modeling and complete lineshape analysis indicated a four site exchange process for which free energies
of activation and free energies could be established. ΔG
‡ values for the barriers of rotation are in the range of 57–59 kJ · mol − 1 at 273 K, which is unusually high for an unsubstituted phenyl group.
Received May 3, 2001. Accepted (revised) June 8, 2001 相似文献
5.
András Székács Éva Lauber Eszter Takács Béla Darvas 《Analytical and bioanalytical chemistry》2010,396(6):2203-2211
The distribution of Cry1Ab toxin was detected in the leaves of genetically modified maize of genetic event MON 810 by enzyme-linked immunosorbent assay. Cry1Ab toxin contents in the leaves at reproductive (milk, R3) phenological stage were
measured to be between 3,878 and 11,148 ng Cry1Ab toxin/g fresh weight. Toxin content was significantly lesser (significant
difference (SD) = 1,823 ng Cry1Ab toxin/g fresh leaf weight, p < 0.01) in leaves at the lowest leaf level, than at higher leaf levels, probably due to partial leaf necrotisation. A substantial
(up to 22%) plant-to-plant variation in Cry1Ab contents in leaves was observed. When studying toxin distribution within the
cross and longitudinal sections of single leaves, lesser variability was detected diagonally, with approximately 20% higher
toxin concentrations at or near the leaf vein. More significant variability (SD = 2,220 ng Cry1Ab toxin/g fresh leaf weight,
p < 0.01) was seen lengthwise along the leaf, starting at 1,892 ng Cry1Ab toxin/g fresh weight at the sheath and rising to
maximum concentration at the middle of the lamella. Cry1Ab toxin content may suffer significant (SD = 2,230 ng Cry1Ab toxin/g
fresh leaf weight, p < 0.01) decreases in the leaf due to necrotisation. The results indicate that the longitudinal dimension of the leaf has
more significance for sampling purposes than the diagonal position. 相似文献
6.
Khalaj-Kondori M Sadeghizadeh M Khajeh K Naderi-Manesh H Ahadi AM Emamzadeh A 《Applied biochemistry and biotechnology》2007,142(2):200-208
Molecular phylogenetic analysis of a novel thermophilic eubacterium isolated from an Iranian hot spring using 16S rDNA sequence
showed that the new isolate belongs to genera Geobacillus. DNA pol I gene from this isolate was amplified, cloned, sequenced, and the three-dimensional (3D) structure of deduced amino
acid sequence was predicted. Sequence analysis revealed the gene is 2,631 bp long, encodes a protein of 876 amino acids with
a calculated molecular mass of 99 kDa, and belongs to family A DNA polymerases. Comparison of 3′–5′exonuclease domain of Klenow
fragment (KF) with corresponding region of newly identified DNA pol I (MF), the large fragment of Bacillus stearothermophilus DNA pol I (BF) and Klentaq1, revealed not only deletions in three regions compared to KF, but that three of the four critical
metal-binding residues in KF (Asp355, Glu357, Asp424, and Asp501) are altered in MF as well. Predicted 3D structure and sequence
alignments between MF and BF showed that all critical residues in the polymerase active site are conserved. 相似文献
7.
Vytautas Getautis Albina Stanisauskaite Rimgaile Degutyte Jolanta Stumbraite 《Monatshefte für Chemie / Chemical Monthly》2007,138(12):1293-1296
Summary. Intramolecular cyclization of N,N′-di(3-chloro-2-hydroxy)propyl-N,N′-diphenylbenzidine occurs to give bis-1,2,3,4-tetrahydroquinoline derivative 1,1′-diphenyl-1,2,3,4,1′,2′,3′,4′-octahydro-6,6′-biquinolinyl-3,3′-diol. 相似文献
8.
Desigan Reddy Kate J. Akerman Matthew P. Akerman Deogratius Jaganyi 《Transition Metal Chemistry》2011,36(6):593-602
The substitution kinetics of the complexes [Pt(terpy)Cl]Cl·2H2O (PtL1), [Pt(tBu3terpy)Cl]ClO4 (PtL2), [Pt{4′-(2′′′-CH3-Ph)terpy}Cl]BF4 (PtL3), [Pt{4′-(2′′′-CF3-Ph)terpy}Cl]CF3SO3 (PtL4), [Pt{4′-(2′′′-CF3-Ph)-6-Ph-bipy}Cl] (PtL5) and [Pt{4′-(2′′′-CH3-Ph)-6-2′′-pyrazinyl-2,2′-bipy}Cl]CF3SO3 (PtL6) with the nucleophiles imidazole (Im), 1-methylimidazole (MIm), 1,2-dimethylimidazole (DIm), pyrazole (Pyz) and 1,2,4-triazole
(Trz) were investigated in a methanolic solution of constant ionic strength. Substitution of the chloride ligand from the metal
complexes by the nucleophiles was investigated as a function of nucleophile concentration and temperature under pseudo first-order conditions using UV/Visible and stopped-flow spectrophotometric techniques. The reactions follow the rate law
k\textobs = k2 [ \textNu ] + k - 2 k_{\text{obs}} = k_{2} \left[ {\text{Nu}} \right] + k_{ - 2} . The results indicate that changing the nature or distance of influence of the substituents on the terpy moiety affects the
π-back-donation ability of the chelate. This in turn controls the electrophilicity of the metal centre and hence its reactivity.
Electron-donating groups decrease the reactivity of the metal centre, while electron-withdrawing groups increase the reactivity.
Placing a strong σ-donor cis to the leaving group greatly decreases the reactivity of the complex, while the addition of a good π-acceptor group significantly
enhances the reactivity. The results indicate that the metal is activated differently by changing the surrounding atoms even
though they are part of a conjugated system. It is also evident that substituents in the cis position activate the metal centre differently to those in the trans position. The kinetic results are supported by DFT calculations, which show that the metal centre is less electrophilic when
a strong σ-donor is cis to the leaving group and more electrophilic when a good π-acceptor group is part of the ring moiety. The temperature dependence
studies support an associative mode of activation. An X-ray crystal structure of Pyz bound to PtL3 was obtained and confirmed
the results of the DFT calculations as to the preferred N-atom as a binding site. 相似文献
9.
Dihydroquercetin (dhq) and its 3-acetate react with acetic anhydride in the absence of a base catalyst to yield mixtures of partially acetylated
products. Three new esters were characterized by NMR spectroscopy as dhq 3,7,3′-triacetate, 3,7,4′-triacetate and 5,7,3′,4′-tetraacetate. At its melting point neat dhq 3,7,3′,4′-tetraacetate is partially converted to dhq 3,3′,4′-triacetate and dhq pentaacetate by intermolecular acetyl transfer. Dhq 7,3′,4′-triacetate yields exclusively dhq 3′,4′-di- and 3,7,3′,4′-tetraacetate under these conditions. The acetylation/deacetylation reactions are accompanied by partial
epimerization: 3 new acetates with 2,3-cis stereochemistry (dhq 3-, 3,7,3′,4′-tetra- and penta-) were identified. Dhq and its 3,7,3′,4′-tetraacetate undergo regiospecific dibromination at C-6 and C-8 with excess N-bromosuccinimide in polar solvents, and 6,8-dibromo-dhq can be regioselectively debrominated to 8-bromo-dhq with sodium sulfite. 相似文献
10.
A. L. Kurlovich V. A. Tarasevich N. G. Kozlov 《Russian Journal of Organic Chemistry》2009,45(10):1503-1508
Condensation of 2′-hydroxy-1,1′: 3′,1″-terphenyl-5′-carbaldehyde with naphthalen-1-amine and cyclohexane-1,3-dione, methyl
2,2-dimethyl-4,6-dioxocyclohexane-1-carboxylate, or dimedone gave the corresponding 7-(2′-hydroxy-1,1′: 3′,1″-terphenyl-5′-yl)-7,8,9,10,11,12-hexahydro-12H-benzo[c]acridin-8-ones. The reaction of 2′-hydroxy-1,1′: 3′,1″-terphenyl-5′-carbaldehyde with naphthalen-1-amine and indan-1,3-dione
produced 7-(2′-hydroxy-1,1′: 3′,1″-terphenyl-5′-yl)-8H-benzo[h]indeno[1,2-b]quinolin-8-one. 7-(2′-Hydroxy-1,1′: 3′,1″-terphenyl-5′-yl)-7,8,9,10,11,12-hexahydrobenzo[b][1,10]phenanthrolin-8-ones were obtained by three-component condensation of 2′-hydroxy-1,1′: 3′,1″-terphenyl-5′-carbaldehyde
with quinolin-8-amine and cyclohexane-1,3-dione, methyl 2,2-dimethyl-4,6-dioxocyclohexane-1-carboxylate, or dimedone. 相似文献
11.
A. V. Timshina S. A. Rubtsova I. N. Alekseev P. A. Slepukhin A. V. Kuchin 《Russian Journal of Organic Chemistry》2010,46(3):363-367
(1R,1′R,2S,4R)-1,7,7-Trimethylspiro[bicyclo[2.2.1]heptane-2,2′-[1,3]dithiolane] 1′-oxide, (1R,2S,3′R,4R)-1,7,7-trimethylspiro[bicyclo[2.2.1]heptane-2,2′-[1,3]dithiolane] 1′,1′,3′-trioxide, and (1R,4R)-1,7,7-trimethylspiro[bicyclo[2.2.1]heptane-2,2′-[1,3]dithiolane] 1′,1′,3′,3′-tetraoxide were synthesized by oxidation of
camphor ethylene dithioacetal with m-chloroperoxybenzoic acid at different substrate-tooxidant ratios. The structure of the products was proved by IR and NMR
spectroscopy and X-ray analysis. 相似文献
12.
E. S. Denislamova N. V. Bubnov A. N. Maslivets 《Russian Journal of Organic Chemistry》2011,47(6):933-936
Methyl 1-aryl-3-benzoyl-4,5-dioxo-4,5-dihydro-1H-pyrrole-2-carboxylates reacted with 6-amino-1,3-dimethylpyrimidine-2,4(1H,3H)-dione to give methyl 11-aryl-12-benzoyl-9-hydroxy-4,6-dimethyl-3,5,10-trioxo-4,6,8,11-tetraazatricyclo[7.2.1.02,7]dodec-2(7)-ene-1-carboxylates which underwent thermal recyclization to 1-aryl-3-benzoyl-4-hydroxy-1′,3′-dimethylspiro[pyrrole-2,5′-pyrrolo[2,3-d]pyrimidine]-2′,4′,5,6′(1H,1′H,3′H,7′H)-tetraones. 相似文献
13.
Three novel tripodal ligands, N,N′,N′′-tri-(3′-phenylpropionic acid-2′-yl-)-1,3,5-triaminomethylbenzene (Ll), N,N′,N′′-tri-(4′-methylvaleric acid-2′-y1-)-1,3,5-triaminomethylbenzene (L2) and N,N′,N′′-tri-(3′methylvaleric acid-2′-yl-)-1,3,5-triaminomethylbenzene (L3), have been synthesized and fully characterized. The
stabilizing ability of complexes of the three ligands with transition metal ions Cu(II), Ni(II), Zn(II) and Co(II) and rare
earth metal ions La(III), Nd(III), Sm(III), Eu(III) and Gd(III) has been investigated by the pontentiometric method in water
and in aqueous KNO3 (0.1 mol dm−3) at 25.0±0.1 °C, respectively. The results show that there is a great deal of difference between two series of complexes’
stabilities. An explanation of the difference has been given. 相似文献
14.
Yu. S. Kosenkova M. P. Polovinka N. I. Komarova D. V. Korchagina S. V. Morozov A. I. Vyalkov N. Yu. Kurochkina V. A. Cheremushkina N. F. Salakhutdinov 《Chemistry of Natural Compounds》2008,44(5):564-568
Flavones 2′,5′-dimethoxyflavone, 3′-methoxy-4′,5′-methylenedioxyflavone, 3′,4′-dimethoxyflavone, 5,6,2′,3′,6′-pentamethoxyflavone,
and 5,6,2′,3′,5′,6′-hexamethoxyflavone; salicylates, methyl-4-methoxysalicylate and peonol; and bisbibenzyl polyphenol riccardin
C were isolated for the first time from the acetone extract of the aerial part of Primula macrocalyx Bge. The content of free and total fatty acids was determined by GC and GC—MS. Palmitic (16:0), octadecatetraenoic 18:4 (6,9,12,15),
linoleic 18:2 (9,12), and α-linolenic 18:3 (9,12,15) were the principal acids from the aerial part of Primula macrocalyx.
Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 457–460, September-October, 2008. 相似文献
15.
Prithwiraj Byabartta 《Transition Metal Chemistry》2005,30(7):804-813
Ag+ assisted aquation of blue cis-trans-cis-RuCl2(RaaiR′)2 (4–6) leads to the synthesis of solvento species, blue-violet cis-trans-cis-[Ru(OH2)2(RaaiR′)2](ClO4)2 [Raai R′=p-R-C6H4
N=N–C3H2–NN–1–R′, (1–3), abbreviated as N,N′-chelator, where N(imidazole) and N(azo) represent N and N′, respectively; R = H (a), OMe (b), NO2 (c) and R′ = Me (1/4/7/10), CH2CH3 (2/5/8/11), CH2Ph (3/6/9/12)] that have been reacted with NO2−in warm EtOH resulting in violet dinitro complexes of the type, Ru(NO2)2(RaaiR′)2 (7–9). The nitrite complexes are useful synthons of electrophilic nitrosyls, and on triturating the compounds, (7b–9b) with conc. HClO4 nitro-nitrosyl derivatives, [Ru(NO2)(NO)(OMeaaiR′)2](ClO4)2 (10b–12b) are isolated. The solution structure and stereoretentive transformation in each step have been established from 1H n.m.r. results. All the complexes exhibit strong MLCT transitions in the visible region. They are redox active and display
one metal-centred oxidation and successive ligand-based reductions. The redox potentials of Ru(III)/Ru(II) (E1/2M) of (10b–12b) are anodically shifted by ∼
∼0.2 V as compared to those of dinitro precursors, (7b–9b). The ν(NO) >1900 cm−1 strongly suggests the presence of linear Ru–NO bonding. The electrophilic behaviour of metal bound nitrosyl has been proved
in one case (12b) by reacting with a bicyclic ketone, camphor, containing an active methylene group and an arylhydrazone with an active methine
group, and the heteroleptic tris chelates thus formed have been characterised. 相似文献
16.
A new chromone-substituted dihydrotriflavonol, (2S,3S)[6-{(3S) 3″,5″-dihydroxy-6″-methoxydihydrochromone}5,3′,4′,5′-tetrahydroxy-7-methoxy-3-O-8-dihydroflavone]2 3-O-8[6-{(3S) 3″,5″-dihydroxy-6″methoxydihydrochromone}3,5,3′,4′,5′-pentahydroxy-7-methoxydihydroflavonol] was isolated from the leaves
of Anogeissus pendula. The structure was determined by UV, 1H NMR, 13C NMR, HMBC, and CD data. 相似文献
17.
Andrei V. Bogdanov Vladimir F. Mironov Lenar I. Musin Rashid Z. Musin Dmitry B. Krivolapov Igor A. Litvinov 《Monatshefte für Chemie / Chemical Monthly》2011,41(3):81-85
Abstract
The reaction of aminomethylisatins (isatin N-Mannich bases) with hexaethylphosphorous triamide leads to the formation of 1,1′-bis(dialkylaminomethyl)-3,3′-bis(indolin-3-ylidene)-2,2′-diones and 1,1′-bis(1,3-dioxo-1,3-dihydroisoindol-2-ylmethyl)-1H,1′H-[3,3′]bisindolylidene-2,2′-dione. 相似文献18.
A. V. Velikorodov O. Yu. Poddubnyi A. K. Kuanchalieva O. O. Krivosheev 《Russian Journal of Organic Chemistry》2010,46(12):1826-1829
1,3-Dipolar cycloaddition to methyl 4-[2-(2-oxo-2,3-dihydro-1H-indol-3-ylidene)-1-oxoethyl]-phenylcarbamate of diazomethane in chloroform-diethyl ether and of 3,4-dimethoxybenzonitrile
oxide generated from the corresponding aldehyde oxime by the action of N-chlorobenzenesulfonamide sodium salt (Chloramine B) in boiling ethanol gave, respectively, methyl 4-(2-oxo-1′,5′-dihydro-1H-spiro[indole-3,4′-pyrazol]-3′-ylcarbonyl)phenylcarbamate and methyl 4-[3′-(3,4-dimethoxyphenyl)-2-oxo-1H,4′H-spiro[indole-3,5′-isoxazol]-4′-ylcarbonyl]phenylcarbamate. The condensation of methyl 4-[2-(2-oxo-2,3-dihydro-1H-indol-3-ylidene)-1-oxoethyl]phenylcarbamate with hydrazine hydrate in ethanol afforded methyl 4-(2-oxo-1,2,2′,4′-tetrahydrospiro[indole-3,3′-pyrazol]-5′-yl)phenylcarbamate. 相似文献
19.
A new approach is suggested for determining the kinetic parameters and rate constants of the elementary steps of reversible
chain reactions having self-acceleration periods due to the long time required for the concentrations of the chain-carrier
radicals to reach their steady-state values. This approach is illustrated by the example of the reversible chain reaction
between N,N′-diphenyl-1,4-benzoquinonediimine and 2,5-dichlorohydroquinone in chlorobenzene. The disappearance rate of one of the initial
reactants, N,N′-diphenyl-1,4-benzoquinonediimine, at the inflection point of its disappearance curve, is considered as the basic kinetic
characteristic of the reaction. The empirical function y = aexp(bt
c
) + d, where a, b, c, and d are the fitted parameters (b < 0, c > 1), is suggested for approximating the S-shaped kinetic curves and for calculating the reaction rate. The rate constants
of the elementary steps are preferably derived from experimental data obtained at equal concentrations of the initial reactants,
and also product additions when their effect on the reaction rate is studied. The effective rate constant of chain termination
is derived from the time to reach the steady state. The results obtained in this way are compared with earlier data obtained
using the “initial” reaction rates calculated by means of exponential approximation of portions of N,N′-diphenyl-1,4-benzoquinonediimine disappearance curves after the inflection point. 相似文献
20.
A new bicyclo[3.2.1]octanoid neolignan rel-(7S,8R,1′S,2′R,3′S)-Δ8′-2′-hydroxy-5,1′,3′-trimethoxy-3,4methylenedioxy-7,3′,8,1′-neolignan (1) was isolated from ethanol extract from the fruit of Ocotea heterochroma Mez & Sodiro ex Mez as well as the known compounds β-friedelanol (2), meso-dehydroguaiaretic acid (3), and yangambin (4), whose structures were elucidated on the basis of their comprehensive spectroscopic analysis including 2D NMR data. Lethality
bioassay using brine shrimp (Artemia salina Leach) was evaluated with the ethanol extract from the Ocotea heterochroma’s fruit. The toxicity of this extract was greater than the toxicity of those fractions obtained in a first solvent partition
(benzene, ethyl acetate, and butanol subfractions) and that of a mixture of acetylated 2′-epimers from the new neolignan 1.
Published in Khimiya Prirodnykh Soedinenii, No. 2, pp. 158–160, March–April, 2009. 相似文献