The biomedical effect of laser-induced photodissociation of oxyhemoglobin in vivo

It is demonstrated that the photodissociation of oxyhemoglobin in cutaneous blood vessels and capillaries allows additional extraction of molecular oxygen, prevents hypoxia, and stimulates aerobic metabolism of cells. On the basis of the studied phenomena, a new optical technology of local oxygenation of tissue directly in the zone of laser irradiation has been developed. It is shown that the efficiency of the proposed method for laser-induced oxygenation of biotissues is comparable with the efficiency of hyperbaric oxygenation, with local action being an additional advantage. Various aspects of the applications of the new technology in modern medicine in which the elimination of local hypoxia is needed are discussed. The proposed optical method for local oxygenation of biotissues makes it possible to solve the hypoxia problem in malignant tissue and substantially increase the efficiency of photodynamic, radiation, and chemical therapy in modern oncology.     

Modeling of optimal conditions for oxyhemoglobin photodissociation in laser-irradiated biotissue

Based on the theory of radiation transfer and a model that describes the structure and optical properties of biotissues, we have found spectral conditions of irradiation of the skin surface that ensure efficient generation of molecular oxygen O₂ in the dermis due to the photodissociation of blood oxyhemoglobin. We show that, for maximal local O₂ formation at depths z ≤ 0.2 mm, 0.2 mm < z ≤ 0.9 mm, 0.9 mm < z ≤ 2.5 mm, and z > 2.5 mm, it is more effective to use wavelengths in the intervals 418 ± 5, 575 ± 5, 585 ± 5, and 600 ± 5 nm, respectively. Physical reasons for the shift of optimal wavelengths toward the red range of the spectrum are described. We show that they are based on the selectivity of optical properties of the skin biotissue, which acts as of a kind of spectral filter the transmission curve of which depends on the depth. It is found that irradiation at a wavelength near 575 nm is optimal for the generation of a maximal amount of O₂ in the intire bulk of the dermis.     

Effect of temperature on the quantum yield of laser-induced photodissociation of oxyhemoglobin in vivo

We have studied the effect of temperature on laser-induced photodissociation of oxyhemoglobin in vivo by recording the change in the oxygen saturation of arterial blood. We have established that on exposure to low-intensity laser radiation, the local concentration of free oxygen in tissue significantly increases for a body temperature above 40°C, compared with the normal temperature. We demonstrate a unique option for selectively and locally increasing the concentration of free molecular oxygen in tissue, which promotes enhancement of cell metabolism. We consider the possibilities for biomedical use of this phenomenon. The results obtained show that the temperature dependence of the quantum yield for photodissociation of oxyhemoglobin should be considered in development of new therapeutic methods for laser medicine. __________ Translated from Zhurnal Prikladnoi Spektroskopii, Vol. 73, No. 1, pp. 90–93, January–February, 2006.     

Interpretation of the Mössbauer spectra of oxyhemoglobin in terms of a low lying excited state

It is shown how the temperature evolution of the Mössbauer spectra of oxyhemoglobin can be explained by means of an excited triplet state at about 150 K above the ground state. The agreement is particularly good for temperatures T ≤ 180 K.     

Laser-induced photodissociation of oxyhemoglobin: Optical method of elimination of hypoxia (oxygen deficiency in biotissue)

We consider the effect of laser-induced in vivo photodissociation of blood oxyhemoglobin on gas exchange in biological tissues. An optical method of laser-induced oxygenation of biotissues is developed and proposed. We show that, in the region of the action of the laser radiation, the degree of oxygenation of a tissue increases. We experimentally confirm that the phenomenon of laser-induced in vivo photodissociation of oxyhemoglobin opens up a new possibility of controlling the local concentration of free molecular oxygen in tissues, eliminating tissue hypoxia, and stimulating aerobic metabolism of cells. We show that the efficiency of the proposed method of laser-induced oxygenation of biotissues proves to be comparable with the efficiency of the hyperbaric oxygenation, but has the advantage of the locality of the action. The proposed optical method of local oxygenation of biotissues will make it possible to eliminate the problem of hypoxia in cancerous tumor tissue and to considerably increase the efficiency of photodynamic, radiation, and chemotherapy in modern oncology.     

Differences of the 57 Fe hyperfine parameters in both oxyhemoglobin and spleen from normal human and patient with primary myelofibrosis

Study of oxyhemoglobin in red blood cells and spleen tissues from normal human and patient with primary myelofibrosis was carried out using Mössbauer spectroscopy with a high velocity resolution. The ⁵⁷Fe hyperfine parameters were evaluated and small variations in quadrupole splitting were revealed for both normal human and patient’s oxyhemoglobin and both normal human and patient’s spleen.     

First principles electronic structure investigation of order of singlet and triplet states of oxyhemoglobin and analysis of possible influence of muon trapping

Interest in the possibility of magnetic character for oxyhemoglobin (OxyHb) has been recently stimulated by the observations of muon spin-lattice relaxation effects studied (Nagamine et al., Proc Jpn Acad Ser B Phys Biol Sci 83:120–126, 2007) with the muon-spin rotation (μSR) technique. In view of this, we have carried out first-principles electronic structure investigations involving Hartree–Fock theory combined with many body perturbation effects for the singlet and triplet states of OxyHb. Our results indicate that using two recent x-ray structural data (Paoli et al., J Mol Biol 256:775, 1996; Park et al., J Mol Biol 360:690, 2006) for OxyHb, for only Hartree–Fock theory without many-body effects included, the singlet state lies above the triplet state by energies of about 0.08 and 0.13 a.u. for the two structures in Paoli et al. (J Mol Biol 256:775, 1996) and Park et al. (J Mol Biol 360:690, 2006). Incorporation of many-body effects by the perturbation method reverses the order, with the triplet state located 0.18 and 0.14 a.u. above the singlet state for the structures in Paoli et al. (J Mol Biol 256:775, 1996) and Park et al. (J Mol Biol 360:690, 2006). Physical reasons for these relative orderings of the singlet and triplet states will be discussed. It is clear that OxyHb by itself would be in a singlet state at room temperature or below, since from our calculation, the triplet state lies about KT above the singlet state with T having the value of 44,098 K and 56,449 K for the two structural data in Paoli et al. (J Mol Biol 256:775, 1996) and Park et al. (J Mol Biol 360:690, 2006). As regards the muon spin-lattice relaxation effects obtained by recent μSR measurements (by Nagamine et al., Proc Jpn Acad Ser B Phys Biol Sci 83:120–126, 2007) at room temperature, the sensitive dependence of the singlet-triplet separation on many-body effects in our investigation suggests that it is possible that the singlet-triplet separation could be reversed or reduced significantly when a muon is trapped near an oxygen atom of the oxygen molecule, allowing the triplet to be occupied at room temperature and lead to significant muon spin-lattice relaxation.     

Investigation of

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Investigation of singularities in general relativity

The problem of singularities for a non-symmetric and isentropic motion of a perfect fluid under the assumption of adiabatic thermodynamic processes is investigated from the standpoint of a local observer. It is shown that, whatever the distribution of matter might be, there occurred a singularity in the past in the non-rotating parts of the universe and it must occur again in the future if the universe is closed. It is further shown that the occurrence of a singularity in a rotating fluid seems inevitable, when the relativistic equation of state is considered, because of extremely high concentration of rest mass, though the possibility of its avoidance may not be ignored.     

Investigation of cadmium-hydrogen complexes in silicon

Formation, stability and electric properties of (In-H) and (Cd-H) complexes in Si are studied by the perturbed angular correlation spectroscopy (PAC). The trapping of hydrogen at the acceptors results in three different defect specific electric field gradients (EFGs), which are studied as function of temperature, doping concentration and annealing time. Two of the three observed hydrogen-related EFGs are identified as different charge states of one (Cd-H) acceptor. Within the framework of a model, which takes into account the dynamics of charge fluctuations and Shockley, Read and Hall statistics, the energy level of this (Cd-H) acceptor at E = E_V + 60 meV is deduced. Furthermore, the dynamical behavior of the hydrogen atom within the (Cd-H) complexes is studied. From the relaxation of the PAC signal the hopping rate of the hydrogen atom is extracted. This rate is thermally activated with an activation energy of E = 0.20 eV.     

钙钛矿锰氧化物中的极化子研究

钙钛矿锰氧化物(以下简称锰氧化物,如La_1-xSr_xMnO₃等,x为掺杂浓度)因其优异的电、磁性质受到人们广泛的关注,但是对于其材料内部载流子性质的认识至今仍没有统一定论.本文基于锰氧化物内Mn—O链的特点,建立一维紧束缚模型,对锰氧化物载流子的性质展开研究.发现在掺杂浓度x=0.5时,系统处于铁磁态,自旋能级完全劈裂,价带和导带之间存在带隙,所有电子态呈现扩展行为.进一步掺杂,将出现局域电子态,同时伴随着晶格的局域畸变,形成所谓的极化子.伴随着极化子的出现,带隙中出现极化子深能级.极化子携带的电荷量越多,形成的晶格缺陷越深,局域能级也越深.当极化子的电荷量继续增加时,极化子解离,载流子倾向于形成能量更低的正反"孤子"对.     

Investigation of anomalons in He fragments

We have studied the mean free path of He fragments from projectile beams of Ar, Fe and Kr of energy 1 ? E ?2A GeV at different distances $\text{(L ? 2.5}\text{cm}\text{)}$ from their production point and found that they are the same, independent of their (i) energy, (ii) target size, (iii) target excitation, (iv) He multiplicities and (v) production angles.     

Investigation of alignment in jet events

The hypothesis previously put forth in the literature that alignment of spots on films that is observed in cosmic-ray emulsion experiments is related to a predominant jet character of events at ultrahigh energies is verified. The Monte Carlo PYTHIA generator, which is known to have provided good results in describing jet events in hadron-hadron interactions, is used in the present analysis. Because of a strong correlation between the directions of jet axes and particle momenta (collinearity) in them, the estimated degree of alignment of spots in jet events is substantially higher than that in a random sample of spots disposed chaotically on an x-ray film. For primary-interaction altitudes and collision energies satisfying some specific constraints, the degree of alignment appears to be strongly dependent on the hardness of the process and on the threshold for the total energy of selected clusters, increasing as they grow.     

Investigation of lateral interactions in chemisorption

It is believed that lateral interactions play an important role in chemisorption site selection. With the advent of angular resolved and polarization dependent photoemission spectroscopies, one is able to compare computed band structure with angular resolved photoemission data. This advance coupled with theoretical calculations provides an excellent opportunity for investigating direct (adsorbate-adsorbate) and indirect (through-substrate) lateral interactions at surfaces. We have modelled the chemisorption of oxygen on Al(111) using direct lateral interactions only (isolated monolayer). The calculated results provide a good framework for interpreting the data. For O₂ monolayer the direct lateral interactions are also appreciable and lead to significant dispersion in the calculated energy bands. We have also computed the electronic structure for two dimensional isolated layers of CO in ordered configurations observed on a Pd(100) surface. The shape and width of the 4σ-derived band is satisfactorily accounted for by this direct lateral interaction only model. Having demonstrated that direct lateral interactions lead to observable band dispersions, it is suggested that they might influence normal vibrational frequencies and ought to be included.     

Investigation of microstrain in dispersion-strengthened steels

Microstrains in three series of the samples made of various stainless austenite dispersion-strengthened steels, which are used to produce different construction parts in reactor engineering, have been studied by high-resolution neutron diffraction. The influence of the temperature and duration of thermal treatment on the precipitation of particles of the dispersion-strengthening phase and on the variation in parameters and microstrains of the crystal lattice has been investigated. An increase in microstrain with the drop of coherence has been observed for all studied steels.     

Marx型脉冲形成网络的研究

介绍了Marx型脉冲形成网络的基本原理, 用PSpice软件对整个脉冲形成网络进行了模拟, 通过优化网络器件参数, 获得了等电容条件下平整的波形输出. 研制了一台4级Marx型脉冲形成网络的脉冲功率源, 在充电电压为20kV的情况下, 在18Ω的匹配负载上获得了40kV的电压输出, 脉冲半高宽为3μs, 平顶宽度2μs.