SKP2和p27kip1在放疗后鼻咽癌CNE2细胞应答中的作用

目的探讨SKP2、p27kip1及细胞周期阻滞在放疗后鼻咽癌细胞应答中的作用.方法以人鼻咽低分化鳞癌CNE2细胞株作为研究对象,流式细胞仪检测放疗前后细胞周期变化,West blot检测放疗前后不同时间点SKP2及p27kip1表达的变化.结果放疗后鼻咽癌CNE2细胞株出现G2/M期细胞周期阻滞,低剂量(2Gy)放疗后0.5~1.5h出现SKP2表达短暂下降,同时伴p27kip1表达相应增高;放疗后4h出现SKP2表达升高而p27kip1表达下调.高剂量(6Gy)放疗后1.5h内出现SKP2表达升高,而放疗后2~4h表达下降;而p27kip1表达在放疗后0.5h出现短暂下降,1.5~4h表达上调,两者之间关联性不确定.结论 SKP2、p27kip1和细胞周期阻滞参与放疗后鼻咽癌CNE2细胞的应答反应,且低剂量放疗时,p27kip1表达可能受SKP2调控.     

浙江地区汉族人群 IL-27p28基因多态性与克罗恩病的相关性研究

目的探讨IL-27p28基因启动子区g.-964 T＞C、第2外显子区g.2905T＞G和第4外显子区g.4730T＞C 3个位点的多态性与浙江地区人群克罗恩病（CD）的相关性。方法选取75例浙江地区汉族CD患者（病例组）及80例健康体检者（对照组）,采用聚合酶链反应-连接酶检测,分析两组基因型频率及等位基因频率。结果对照组的基因型分布符合Hardy- Weinberg平衡定律。两组g.-964位点的基因型频率及等位基因频率的差异有统计学意义（P＜0.05）,而g.2905和g.4730位点的基因频率及等位基因频率的差异无统计学意义（P＞0.05）。结论 IL-27p28基因多态位点g.-964 T＞C可能与浙江地区汉族人群CD易感性有关, g.2905T＞G和g.4730T＞C多态性与浙江地区汉族人群CD无关。     

胆囊癌中PTEN基因异常表达及甲基化的研究

目的 探讨胆囊癌的发生、发展及转移与PTEN 基因异常表达及其基因启动子区异常甲基化的关系。方法 选取胆囊癌标本44 例（胆囊癌组）和胆囊炎标本20 例（对照组）,通过免疫组织化学染色、Western-blot、甲基化特异性PCR法分析两组患者胆囊组织中PTEN 基因表达及启动子区甲基化差异。结果 与对照组相比,胆囊癌组中PTEN 基因表达阳性率明显下降（P＜0.01）,而启动子区甲基化阳性率显著增高（P＜0.01）;胆囊癌组中有淋巴结转移者PTEN 基因表达阳性率较无淋巴结转移者降低（P＜0.05）,而启动子区甲基化阳性率增高（P＜0.05）;胆囊癌分期增高,PTEN 基因表达阳性率降低（P＜0.05）,而启动子区甲基化阳性率增高（P＜0.05）。结论 PTEN 基因低表达是胆囊癌的发生、发展及转移的重要原因之一,其低表达与启动子区过度甲基化有关。     

VEGF、EGFR 及Parkin 蛋白在 鼻咽癌中的表达及三者间的相关性研究

目的 探讨血管内皮生长因子（VEGF）、表皮生长因子受体（EGFR）及Parkin 蛋白在鼻咽癌（NPC）中的表达情况以 及三者间的相关性。方法 应用免疫组化SP 法检测48 例NPC 组织和34例正常鼻咽部上皮组织中VEGF、EGFR 及Parkin蛋白的表达,并探讨三者间的相关性。结果 NPC组织中VEGF和EGFR蛋白表达阳性率明显高于正常鼻咽部上皮组织,Parkin蛋白表达阳性率则明显下降,且均有统计学差异（均P＜0.05）。NPC组织中VEGF蛋白表达与NPC患者有无淋巴结转移及临床TNM 分期密切相关（均P＜0.05）,而与患者的年龄、性别、病理类型、T 分期均无关（均P ＞0.05）;EGFR 和Parkin 蛋白表达与NPC 患者有无淋巴结转移密切相关（均P＜0.05）,而与患者的年龄、性别、病理类型、T分期、临床TNM分期均无关（均P ＞0.05）。NPC组织中VEGF和EGFR 蛋白的表达呈正相关（P＜0.05）,Parkin和VEGF、EGFR蛋白的表达无相关性（均P＞0.05）。结论 VEGF、EGFR 及Parkin 蛋白与NPC发生、发展有关,VEGF、EGFR 及Parkin蛋白表达可作为NPC 的预后判断指标,NPC 中VEGF 和EGFR可能起协同作用,而Parkin 则可能单独发挥作用。     

𝒱-Nilpotent Groups and 5-Term Exact Sequence

In this paper, we prove that any nontrivial normal subgroup N of 𝒱-nilpotent group G, intersects nontrivially with the marginal subgroup of G with respect to the variety 𝒱. This is a generalization of a well-known theorem by Philip Hall which refers to the case where 𝒱 is the variety of abelian groups. Moreover, we give a generalized version of a 5-term exact sequence of homology groups and establish some isomorphisms of the factors of the lower marginal series.     

TFF1在肺癌中的表达及作用研究

目的 研究三叶因子1（TFF1）在肺癌组织及肺癌细胞株中的表达、甲基化状态及其功能。方法 选取98 例非小细胞肺癌患者手术切除的肺癌组织,采用RT-PCR、MSP 检测TFF1 在肺癌组织中的表达与甲基化状态,分析其与患者临床特点的相关性,以及检测肺癌细胞株H23、H1299、L78、H446、H157 及95D 中TFF1 的表达和甲基化状态以及5-aza-2''-deoxycytidine（DAC）处理后细胞株中TFF1 表达的变化。TFF1 转染H23 细胞株,MTT 法及克隆形成实验检测其增殖能力和克隆能力的变化,细胞凋亡实验及Western blot 检测凋亡相关蛋白Caspase3 表达水平。结果 TFF1 在肺癌组织中的甲基化率为56.1%（55/98）。TFF1 甲基化与肺癌患者的TNM 分期和淋巴结转移显著相关（P＜0.001）。TFF1 在H23 和H157 中无表达,在H1299、H446 及95D 中表达较低,在L78 中表达较高。DAC 处理后,细胞株TFF1 表达的变化分别为表达恢复（H23、H157）,表达增强（H1299,H446,95D）和无明显改变（L78）。恢复表达TFF1 后,H23 细胞株的增殖及克隆形成能力明显减弱（P＜0.05）,细胞凋亡率和凋亡相关蛋白Caspase3 活性片段的表达明显增加。结论 TFF1 在肺癌组织中的甲基化状态与肺癌的TNM 分期和转移密切相关。TFF1 在肺癌细胞中的表达受DNA 甲基化的调控。肺癌细胞株H23 中恢复表达TFF1 可以抑制其增殖和克隆能力,促进其凋亡。     

A locally and cubically convergent algorithm for computing 𝒵‐eigenpairs of symmetric tensors

This paper is concerned with computing $\mathrm{??}$‐eigenpairs of symmetric tensors. We first show that computing $\mathrm{??}$‐eigenpairs of a symmetric tensor is equivalent to finding the nonzero solutions of a nonlinear system of equations, and then propose a modified normalized Newton method (MNNM) for it. Our proposed MNNM method is proved to be locally and cubically convergent under some suitable conditions, which greatly improves the Newton correction method and the orthogonal Newton correction method recently provided by Jaffe, Weiss and Nadler since these two methods only enjoy a quadratic rate of convergence. As an application, the unitary symmetric eigenpairs of a complex‐valued symmetric tensor arising from the computation of quantum entanglement in quantum physics are calculated by the MNNM method. Some numerical results are presented to illustrate the efficiency and effectiveness of our method.     

多梳基因Bmi-1 在甲状腺乳头状癌中的表达及意义

目的 研究多梳基因Bmi-1 在甲状腺乳头状癌组织中的表达及临床意义。方法 分别采用免疫组化法、Westernblot 法和RT-PCR 法检测30 例甲状腺乳头状癌组织、30 例结节性甲状腺肿组织及30 例正常甲状腺组织中多梳基因Bmi-1 及其mRNA 的表达情况,并分析Bmi-1与甲状腺乳头状癌临床病理特征的关系。结果 免疫组化法检测发现Bmi-1 在甲状腺乳头状癌组织中阳性表达率73.3%,与结节性甲状腺肿（36.7%）及与正常组织（16.7%）的阳性表达率相比差异有统计学意义（P＜0.05）。Western blot 法检测甲状腺乳头状癌中Bmi-1 表达量与结节性甲状腺肿组织及正常组织相比,差异有统计学意义（P＜0.05）。RT-PCR法检测甲状腺乳头状癌中Bmi-1mRNA 表达量与结节性甲状腺肿组织及正常组织相比,差异有统计学意义（P＜0.05）。Bmi-1 阳性表达在甲状腺乳头状癌有无淋巴结转移中的差异有统计学意义（P＜0.05）,在年龄、性别、肿瘤大小、分期等差异无统计学意义。结论 Bmi-1 在甲状腺乳头状癌中表达升高,可能在甲状腺乳头状癌的发生、发展过程中起重要作用,并可望作为甲状腺乳头状癌临床诊断的标记物之一。     

Neuropilin-1与胃癌发生、发展及血管生成的关系

目的探讨胃癌组织中Neuropilin-1、VEGF的表达与胃癌生物学行为及血管生成的关系和意义.方法应用免疫组化ElivisionTM法检测正常胃黏膜、上皮内瘤变、胃癌组织中Neuropilin-1、VEGF、CD34的表达并测定MVD,回顾分析胃癌患者的临床病理资料.结果胃癌组织中Neuropilin-1、VEGF的表达及MVD高于正常胃黏膜和上皮内瘤变组织,且与胃癌淋巴结转移、浸润程度密切相关.Neuropilin-1、VEGF表达阳性组的MVD高于各自阴性组.两者表达呈正相关关系,联合表达时MVD增高.结论 Neuropilin-1、VEGF、血管生成参与了胃癌发生、浸润与转移;Neuropilin-1、VEGF参与了胃癌的血管生成;表达存在协同作用;联合表达时血管生成的效应增强.Neuropilin-1作为肿瘤抗血管治疗的靶点具有一定的价值.     

在空间W_0~(1，x)L~(P(x))(Q)及其共轭空间中的若干收敛性定理

本文证明了在空间W_0~(1,x)L~(p(x))(Q)及其共轭空间中三个收敛性定理.在附加较弱条件的情况下,给出了几乎处处收敛蕴含弱收敛,弱收敛蕴含强收敛.     

Mathematical description of the interactions of CycE/Cdk2, Cdc25A,and P27Kip1 in a core cancer subnetwork

Recent studies have shown that the initiation of human cancer is due to the malfunction of some genes (such as E2F, CycE, CycD, Cdc25A, P27^Kip1, and Rb) at the R‐checkpoint during the G₁‐to‐S transition of the cell cycle. Identifying and modeling the dynamics of these genes provide new insight into the initiation and progression of many types of cancers. In this study, a cancer subnetwork that has a mutual activation between phosphatase Cdc25A and the CycE/Cdk2 complex and a mutual inhibition between the Cdk inhibitor P27^Kip1 and the CycE/Cdk2 complex is identified. A new mathematical model for the dynamics of this cancer subnetwork is developed. Positive steady states are determined and rigorously analyzed. We have found a condition for the existence of positive steady states from the activation, inhibition, and degradation parameter values of the dynamical system. We also found a robust condition that needs to be satisfied for the steady states to be asymptotically stable. We determine the parameter value(s) under which the system exhibits a saddle–node bifurcation. We also identify the condition for which the system exhibits damped oscillation solutions. We further explore the possibility of Hopf and homoclinic bifurcations from the saddle–focus steady state of the system. Our analytic and numerical results confirm experimental results in the literature, thus validating our model. Copyright © 2016 John Wiley & Sons, Ltd.     

Magnetovolume and chemical bonding effects of Ni atom in γ’-(Fe1- x Ni x )4N compounds

By X-ray diffraction and high pressure Mossbauer spectroscopy, the structure and the hyperfine parameters of Ni substituted γ’-Fe₄N were investigated. The results of X-ray diffraction indicate that single phase γ’-(Fe₁-_xNi_x)₄N compounds can be prepared in the composition range of 0⩽x⩽0.6, and with the increase of Ni content the lattice parameter is fit for the relationshipa ₀(x)=3.790 5-0.021 57x-0.031 67x ². By high pressure Mossbauer spectra, effects of magnetovolume and chemical bonding of Ni atom on hyperfine magnetic field and isomer shift of iron were distinguished for the first time, and their composition dependences for different lattice sites were studied simultaneously. It is found that the magnetovolume and chemical bonding have different influences on the properties of γ’-(Fe₁-_xNi_x)₄N, and the latter one plays a key role in the property changes of γ’-(Fe₁-_xNi_x)₄N. Project supported by the National Natural Science Foundation of China, the Natural Science Fund of Gansu Province and the Postdoctoral Fund of China.