共查询到20条相似文献,搜索用时 62 毫秒
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蛋白酶体涉及机体的多种生理功能和许多疾病的发病机制.近年来的研究发现,金属配合物尤其是铜的配合物对肿瘤细胞中蛋白酶体活性有较强的抑制作用并能诱导肿瘤细胞凋亡.本文综述了金属配合物作为蛋白酶体抑制剂及肿瘤细胞凋亡诱导剂的研究进展. 相似文献
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特异识别和切割DNA的过渡金属配合物 总被引:4,自引:0,他引:4
利用过渡金属配合物及其载体衍生物识别和切割DNA是近十年来发展起来的新型分子生物学和基因工程工具试剂。作为DNA构象变化的探讨,DNA-蛋白质相互作用分析的足迹试,或基因组和染色体作图和测试的工具试剂已受到了广泛极大重视。 相似文献
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碱金属、碱土金属、希土元素、锕系元素的阳离子与冠醚形成配合物的报导已有许多,但过渡金属Mn、Co、Ni、Cu的二价阳离子与冠醚形成配合物的报导却很少,而且不成系统,还未见到Fe~(3+)、Cr~(8+)与冠醚形成配合物的报导。我们以12-冠-4(Ⅰ)、15-冠-5(Ⅱ)、18-冠-6(Ⅲ)为配体,制备了Fe~(3+)、Cr~(3+)、Mn~(2+)、Co~(2+)、Ni~(2+)Cu~(2+)的硝酸盐和氯化物两系列配合物。 相似文献
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富勒烯的金属配合物及其催化性能 总被引:5,自引:0,他引:5
富勒烯的金属配合物及其催化性能1)刘英陈远荫2)盛蓉生(武汉大学化学系武汉430072)关键词富勒烯金属配合物催化分类号O643.32碳元素的第三种存在形式富勒烯正以其独特的结构和性能以及诱人的应用前景引起人们日益浓厚的兴趣.其中又以C60的研究为主... 相似文献
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荧光法研究手性金属配合物与DNA的作用机理 总被引:27,自引:2,他引:27
以溴化乙锭为荧光探针,研究手性金属配合物[Ni(phen)3]^+和[Fe(phen)3]^2+与DNA的反应机理。结果表明,配合物与DNA作用存在插入和静电结合两种模式,即部分菲咯啉配体插入DNA双螺旋碱基对中,同时带正电荷的配合物与DNA的磷酸基团发生静电结合。 相似文献
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Schiff碱,咪唑金属配合物合成及抗癌活性的荧光筛选研究 总被引:3,自引:0,他引:3
本文报道了一类新的Schiff碱和唑的混合型金属配合物(即水杨醛缩甘氨酸,咪唑金属配合物和2.4-二羟基苯甲醛缩丙氨酸,咪唑金属配合物)的合成,以及用溴化乙锭荧光分析法对这类配合物与DNA相互作用的研究。结果表明,其中金属镍配合物与DNA的作用效明显,可作为具有抗癌作用的药物进行深入研究。 相似文献
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XIAO Suyao LIU Xuanming TONG Chunyi LIU Jun TANG Dongying & ZHAO Lijian Institute of Life Science Biotechnology College of Chemistry & Chemical Engineering Hunan University Changsha China Correspondence should be addressed to Liu Xuanming 《中国科学B辑(英文版)》2005,48(2)
The gene carrier system is the key factor in genetransfection and gene therapy. Suitable gene carriercan deliver the target gene into the receptor cells safely,highly efficiently, controllably, and then the gene isexpressed, thus accomplishing the gene tr… 相似文献
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Anion starch nanoparticle (StNP) with a diameter of 50 nm was prepared in water-in-oil microemulsion, with soluble starch
as raw materials and POCl3 as crosslinking agent. PLL-StNP was prepared by linking poly-L-lysine (PLL) on the surface of StNP. At the same time, the
size of PLL-StNP and its stability in aqueous solution were checked by AFM. The analysis of plasmid DNA binding, DNase I enzymatic
degradation, toxicity and transfection were done. We discovered that PLL-StNP may be used as non-virus nanoparticle gene carrier.
And we developed the method of preparing PLL-StNP gene carrier and used it in cell transfection. As non-virus gene carrier,
PLL-StNP has some advantages, such as large load of DNA, high transfection efficiency, low cell toxicity and biodegradability. 相似文献
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The transfer of nucleic acids (DNA or RNA) into living cells, that is, transfection, is a major technique in current biochemistry and molecular biology. This process permits the selective introduction of genetic material for protein synthesis as well as the selective inhibition of protein synthesis (antisense or gene silencing). As nucleic acids alone are not able to penetrate the cell wall, efficient carriers are needed. Besides viral, polymeric, and liposomal agents, inorganic nanoparticles are especially suitable for this purpose because they can be prepared and surface-functionalized in many different ways. Herein, the current state of the art is discussed from a chemical viewpoint. Advantages and disadvantages of the available methods are compared. 相似文献
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The chemical structure of methylated poly(1‐vinylimidazole) (PVIm‐Me) has been tuned for gene carrier properties, which are cell viability, the stability of its DNA polyion complexes, and gene expression. The resulting PVIm‐Me with lower methylation degree (6 and 20 mol%) exhibited no significant cytotoxicity, as compared with 40 mol% PVIm‐Me, in spite of the same concentration and chemical structure of positive charges. The DNA complex with 20 mol% PVIm‐Me stably retained the DNA, as compared with 6 or 40 mol% PVIm‐Me, which was examined by competitive exchange with dextran sulfate. As a result, the DNA complex with 20 mol% PVIm‐Me mediated most efficient gene delivery, where the resulting transfection activity was higher than that mediated by a positive control, poly(ethylenimine). These results suggest that the optimization of the balance of the methylimidazolium and the imidazole groups of PVIm‐Me is essential for gene carrier design. Copyright © 2014 John Wiley & Sons, Ltd. 相似文献
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Yang Y Jia W Qi X Yang C Liu L Zhang Z Ma J Zhou S Li X 《Macromolecular bioscience》2004,4(12):1113-1117
This study investigated two new biodegradable polymers as gene controlled-released coatings for gene transfer. Poly(ethylene glycol)-co-poly(D,L-lactic acid) (PELA) and poly(ethylene glycol)-co-poly(lactic acid)-co-poly(glycolic acid) random copolymer (PELGA) were synthesized and used as microspheres matrices with encapsulated plasmid pCH110. The plasmid loading efficiency, cytotoxicity, transfection efficiency and in vitro degradation and release profiles of microsphere complexes were evaluated in details. The biodegradable polymers showed high DNA loading efficiency and low cytotoxicity as gene controlled-released coatings, and the poly(ethylene glycol) (PEG) contents of polymer matrices influenced the diameter, loading efficiency and transfection efficiency of plasmid DNA within the microspheres. The average diameters of PELA and PELGA microspheres were between 0.5 and 1.5 microm, and the plasmid loading efficiency was 62 and 73% for PELA and PELGA microspheres with 10% PEG content, respectively. In vitro testing showed a gradual release profile of DNA from polymeric matrices. The polymers/DNA microspheres had high transfection efficiency and early gene expression and maintenance of gene expression level for up to 96 h, although transfection efficiency were slightly lower than that of liposome in the initial 24 h. The biodegradable polymeric materials possess potential superiority as gene carriers. 相似文献
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基因治疗通过基因载体将治病基因导入病患的特异细胞以治疗心血管、神经系统疾病和癌症等。寻找安全高效的非病毒基因载体一直是基因治疗以及生物材料领域中的前沿课题。聚酰胺-胺型(PAMAM)树枝状高分子作为一类三维的、结构高度有序的新型载体,由于具有安全性好、易于修饰、携带外源基因容量大等特点,已经引起了广泛的关注。但是另一方面,合成步骤相对繁琐、后期产物纯化困难以及转染效率相对较低等问题限制了这类载体的进一步发展。本文结合本课题组的研究情况,针对如何提高PAMAM的转染效率以及增强其基因传递的靶向性等相关问题,对近几年在PAMAM树枝状分子修饰改性方面所做的一些有意义的工作进行了综述,并对前景进行了展望。 相似文献
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Tomoko Hashimoto Reiko Iwase Akira Murakami Tetsuji Yamaoka 《Polymer Degradation and Stability》2009,94(9):1349-1353
Novel biodegradable oligopeptide-type gene carriers composed of cationic residues (KRRRKRKRRRKRKRRC) and oligo leucine segments were developed. The amphiphilic carrier was found to form micelle-like assemblies in aqueous solutions, when the oligo leucine is 12 amino acids length (Pep-L12). NMR, CMC, and GPC analysis revealed their hydrophobic/cationic core/shell morphology. Hydrophobic interaction between leucines is thought to be the major driving force behind formations of assemblies. The transient expression of luciferase introduced to COS-1 cells using Pep-L12 below the CMC is as low as that by the control cationic peptides without leucine residue (Pep-L0), while improved transgene expression was observed in the case of Pep-L12 above CMC. The self-assembly raised the apparent molecular weight and gene transfection ability without loosening their low cytotoxicity. These results indicate that the amphiphilic oligopeptides are very promising materials as highly efficient and less toxic gene carriers. 相似文献