钴离子配位分子印迹聚合物膜渗透特性的研究

采用分子印迹技术紫外光引发原位聚合的方法制备了带支撑膜的钴离子配位分子印迹聚合物膜. 用扫描电镜测定了膜的表面形貌. 通过膜渗透实验表明, 在一定浓度钴离子存在下, 印迹膜对模板分子表现出良好的渗透选择性. 分别考察了阳离子和阴离子对印迹膜渗透模板分子的影响. 本工作有助于分子印迹技术应用于传感器技术和连续分离技术的研究.     

锌-槲皮素配位分子印迹聚合物膜渗透特性的研究

采用紫外光引发原位聚合的方法制备了以聚偏氟乙烯管状微孔滤膜为支撑膜的锌离子配位分子印迹聚合物膜. 通过膜渗透实验表明, 在一定浓度锌离子存在下, 印迹膜对模板分子槲皮素表现出良好的渗透选择性. 分别考察了阳离子和阴离子对印迹膜渗透模板分子的影响. 本工作对分子印迹技术应用于实际样品中槲皮素的分离与富集具有重要意义.     

槲皮素金属配位印迹聚合物膜的制备及性能研究

以中药活性成分黄酮类化合物槲皮素与Zn(II)的配合物为模板, 以4-乙烯基吡啶为功能单体, 乙二醇二甲基丙烯酸酯为交联剂, 水溶性线性聚合物聚乙二醇(PEG, M_w＝600)为添加剂, 利用分子印迹技术紫外光引发原位聚合的方法制备了带支撑的锌离子配位分子印迹聚合物膜. 使用扫描电镜测定了不同PEG用量下膜的表面形貌. 通过膜渗透实验考察了PEG含量对金属配位印迹膜渗透速率的影响, 系列印迹聚合物膜的识别体系及对不同底物的渗透选择性, 同时考察了阳离子和阴离子对印迹膜渗透模板分子的影响. 实验结果表明: 制膜过程中水溶性线性聚合物PEG的加入可以明显改善膜的透过性能|槲皮素-Zn(II)模板印迹聚合物膜对槲皮素表现出明显的渗透选择性, 对槲皮素结构类似物芦丁和柚皮素的渗透选择性较差|且该金属配位印迹聚合物膜对不同阳离子和阴离子也都表现出较好的选择识别作用.     

重金属离子印迹技术

分子印迹技术(molecular imprinting technology,MIT)是指制备对特定目标分子具有专一识别性能的聚合物技术。离子印迹技术(ion imprinting technology,IIT)以离子为模板,通过静电作用、配位作用等与单体结合形成螯合物,聚合后用酸性试剂等将模板离子洗脱,最终制得具有与目标金属离子相对应的三维孔穴结构的印迹材料。作为分子印迹技术的重要分支,离子印迹技术因存在配位作用而具有很多优势,近年来得到了快速的发展。重金属离子是离子印迹领域最典型且最受关注的目标物。本文介绍了离子印迹技术原理、制备及其在金属的痕量和超痕量分析中的优势,针对环境监测中典型重金属污染离子(铅、汞、铜、镉、铬、砷)的印迹聚合物应用进行了简述,并对金属离子印迹技术未来的挑战与发展作出了展望。     

合成引发方式对槲皮素-钴(Ⅱ)配位印迹聚合物结构与性能的影响

以槲皮素-钴(Ⅱ)的配合物为模板分子,在强极性甲醇溶剂中分别采用低温光引发和高温热引发聚合制备槲皮素-钴(Ⅱ)配位印迹聚合物.紫外-可见光谱分析确定了槲皮素与钴(Ⅱ)形成配合物的最佳配位比.根据印迹聚合物的平衡结合量优化功能单体丙烯酰胺用量.利用红外光谱、透射电镜和平衡结合实验,考察不同引发方式对聚合物的结构、微观形貌及结合性能的影响.进一步通过特异吸附容量和印迹指数确定,低温光引发聚合更适于配位分子印迹聚合物的制备.同时研究了不同阳、阴离子对印迹聚合物选择识别性的影响.结果表明光引发的金属配位分子印迹聚合物具有良好的吸附选择性,印迹指数可达3.919.     

磁性离子印迹聚合物的制备研究进展

离子印迹技术是分子印迹技术的一个重要分支。离子印迹聚合物具有预定识别性,制备简单、价格低廉、稳定性好,对模板离子表现出较高的选择性和亲和性等优点,但离子印迹材料在完成吸附使命后从反应体系中分离困难。将离子印迹技术与磁性技术相结合制备的磁性离子印迹聚合物,不仅具有特定的分子识别位点,而且具有磁响应特性,在外加磁场作用下,容易分离回收。此文综述了近年来磁性离子印迹聚合物的研究进展状况,同时提出了目前该领域存在的问题和发展趋势。     

镍离子(Ⅱ)印迹聚合物的制备及性能研究

以镍(Ⅱ)离子为模板,丙烯酰胺为功能单体,二乙烯基苯为交联剂,偶氮二异丁腈为引发剂,甲醇为溶剂,采用本体聚合法制备了镍离子(Ⅱ)印迹聚合物.用红外光谱对镍离子印迹聚合物的结构进行表征,红外光谱研究表明聚合物中存在与模板分子相互作用的特征基团.通过研究Ni2+与单体的物质的量之比、单体与交联剂的物质的量之比、溶剂量对镍离...     

配合物印迹模板的制备及对铜离子的吸附性能

以阿司匹林铜配合物为目标分子,4-乙烯基吡啶为功能单体,乙二醇二甲基丙烯酸酯为交联剂,偶氮二异丁腈为引发剂制备了阿司匹林铜配合物印迹聚合物.采用EDTA洗脱铜离子,再用索氏提取洗脱阿司匹林后得到两种印迹模板,并用铜试剂分光光度法研究了两种分子印迹模板对铜离子的吸附时间、吸附等温线和Scatchard方程曲线的影响.结果表明,未洗脱阿司匹林的印迹模板比洗脱阿司匹林的印迹模板的吸附性能较佳,但差异不够显著.     

铅(Ⅱ)离子印迹聚合物的制备及其吸附性能的研究

利用分子印迹技术制备了重金属铅(Ⅱ)的印迹聚合物,并对其识别性能进行定量描述.以铅(Ⅱ)离子为模板,水杨醛肟为功能单体,二甲基丙烯酸乙二醇酯为交联剂,偶氮二异丁腈为引发剂,采用本体聚合法制备印迹聚合物.结果表明,与未印迹的聚合物相比较,印迹聚合物对铅(Ⅱ)离子具有较好的识别选择性,达到吸附平衡的时间为40min,Pb2...     

以分子印迹聚合物为固定相分离和测定氟喹诺酮类药物

以氧氟沙星作为模板分子合成了分子印迹聚合物,并通过高效液相色谱法研究了印迹聚合物的识别特性。实验结果表明,印迹聚合物对模板分子具有很强的亲和力和特定的选择性。作为色谱固定相,氧氟沙星印迹聚合物和目标分子之间的相互作用除了印迹部分的离子和氢键作用外,也存在非印迹部分的疏水作用。同时研究了色谱条件对氟喹诺酮类药物分离的影响。     

The effectively specific recognition of bovine serum albumin imprinted silica nanoparticles by utilizing a macromolecularly functional monomer to stabilize and imprint template

Structural stability of the template is one of the most important considerations during the preparation of protein imprinting technology. To address this limitation, we propose a novel and versatile strategy of utilizing macromolecularly functional monomers to imprint biomacromolecules. Results from circular dichroism and synchronous fluorescence experiments reflect the macromolecularly functional monomers tendency to interact with the protein surface instead of permeating it and destroying the hydrogen bonds that maintain the protein’s structural stability, therefore stabilizing the template protein structure during the preparation of imprinted polymers. The imprinted polymers composed of macromolecularly functional monomers or their equivalent micromolecularly functional monomers over silica nanoparticles were characterized and carried out in batch rebinding test and competitive adsorption experiments. In batch rebinding test, the imprinted particles prepared with macromolecularly functional monomers exhibited an imprinting factor of 5.8 compared to those prepared by micromolecularly functional monomers with the imprinting factor of 3.4. The selective and competitive adsorption experiments also demonstrated the imprinted particles made by macromolecularly functional monomers possessed much better selectivity and specific recognition ability for template protein. Therefore, using macromolecularly functional monomers to imprint may overcome the mutability of biomacromolecule typically observed during the preparation of imprinted polymers, and thus promote the further development of imprinting technology.     

One‐step preparation of magnetic imprinted nanoparticles adopting dopamine‐cupric ion as a co‐monomer for the specific recognition of bovine hemoglobin

A novel magnetic core–shell polydopamine–cupric ion complex imprinted polymer was prepared in one‐step through surface imprinting technology, which could specifically recognize bovine hemoglobin from the real blood samples. The polymerization conditions and adsorption performance of the resultant nanomaterials were investigated in detail. The results showed that the cupric ion played an important role in the recognition of template proteins. The saturating adsorption capacity of this kind of imprinted polymers was 2.23 times greater than those of imprinted polymers without cupric ion. The imprinting factor of the imprinted materials was as high as 4.23 for the template molecule. The selective separation bovine hemoglobin from the real blood sample is successfully applied. In addition, the prepared materials had excellent stability and no obvious deterioration after five adsorption–regeneration cycles. Easy preparation, rapid separation, high binding capacity and satisfactory selectivity for the template protein make this polymer attractive in the separation of high‐abundance proteins.     

分子印迹技术在生物大分子分离识别中的应用

分子印迹技术是近些年发展起来的模拟抗体-抗原相互作用原理的新技术。该文介绍了分子印迹技术的产生和发展,重点介绍了生物大分子印迹聚合物的制备条件、聚合方法及其识别机理,并对该技术的应用前景及目前存在的问题进行了探讨。     

Molecular imprinting of protein by coordinate interaction

In this article,a novel strong interaction by forming complex between bovine serum albumin(BSA) and copper ion was utilized for the preparation of molecular imprinted hydrogel in aqueous solution.Results show that the inclusion of copper ion in preparation can bridge the template BSA and functional monomers together and improve the imprinting effect compared to the polymer made without copper ion added.High selectivity factor and large adsorption capacity are also observed for the obtained BSA-imprinted ...     

Chromatographic characteristics of cholesterol-imprinted polymers prepared by covalent and non-covalent imprinting methods

Cholesterol-imprinted polymers were prepared in bulk polymerization by the methods of covalent and non-covalent imprinting. The former involved the use of a template-containing monomer, cholesteryl (4-vinyl)phenyl carbonate, while the latter used the complexes of template and functional monomer, methacrylic acid or 4-vinylpyridine prior to polymerization. Columns packed with these molecularly imprinted polymers (MIPs) were all able to separate cholesterol from other steroids. For different combinations of cholesterol and beta-estradiol concentrations in a total of 1 g/l, the peak retention times for both compounds were nearly constant. The adsorption capacity for cholesterol onto the MIPs was found to significantly depend on the use of functional monomers, but the selectivity factors were only slightly different from each other at 2.9 to 3.2 since the separation was all based on the specific binding of cholesterol to recognition sites formed on the imprinted polymers. The capacity factors for cholesterol were determined to be 3.5, 4.0 and 3.1, respectively, for covalently imprinted, 4-vinylpyridine-based, and methacrylic acid-based non-covalently imprinted polymers. However, the covalently imprinted polymer was found to have a higher adsorption capacity for cholesterol and about fivefold higher chromatographic efficiency for cholesterol separation, in comparison with non-covalently imprinted polymers. The use of covalent imprinting significantly reduced the peak broadening and tailing. This advantage along with constant retention suggests that the covalently imprinted polymer has potential for quantitative analysis.     

State‐of‐the‐art applications of cyclodextrins as functional monomers in molecular imprinting techniques: a review

As a versatile tool in separation science, cyclodextrins and their derivatives, known as emerging functional monomers, have been used extensively in molecular imprinting techniques. The attributes of cyclodextrins and their derivatives are widely known to form host–guest inclusion complex processes between the polymer and template. The exploitation of the imprinting technique could produce a product of molecularly imprinted polymers, which are very robust with long‐term stability, reliability, cost‐efficiency, and selectivity. Hence, molecularly imprinted polymers have gained popularity in chemical separation and analysis. Molecularly imprinted polymers containing either cyclodextrin or its derivatives demonstrate superior binding effects for a target molecule. As noted in the previous studies, the functional monomers of cyclodextrins and their derivatives have been used in molecular imprinting for selective separation with a wide range of chemical compounds, including steroidals, amino acids, polysaccharides, drugs, plant hormones, proteins, pesticides, and plastic additives. Therefore, the main goal of this review is to illustrate the exotic applications of imprinting techniques employing cyclodextrins and their derivatives as single or binary functional monomers in synthesizing molecularly imprinted polymers in areas of separation science by reviewing some of the latest studies reported in the literature.     

Selective enrichment and separation of phosphotyrosine peptides by thermosensitive molecularly imprinted polymers

Novel thermosensitive molecularly imprinted polymers were successfully prepared using the epitope imprinting approach in the presence of the mimic template phenylphosphonic acid, the functional monomer vinylphosphonic acid‐Ti⁴⁺, the temperature‐sensitive monomer N‐isopropylacrylamide and the crosslinker N,N′‐methylenebisacrylamide. The ratio of the template/thermosensitive monomers/crosslinker was optimized, and when the ratio was 2:2:1, the prepared thermosensitive molecularly imprinted polymers had the highest imprinting factor. The synthetic thermosensitive molecularly imprinted polymers were characterized by Fourier transform infrared spectroscopy to reveal the combination and elution processes of the template. Then, the adsorption capacity and thermosensitivity was measured. When the temperature was 28°C, the imprinting factor was the highest. The selectivity and adsorption capacity of the thermosensitive molecularly imprinted polymers for phosphotyrosine peptides from a mixture of three tailor‐made peptides were measured by high‐performance liquid chromatography. The results showed that the thermosensitive molecularly imprinted polymers have good selectivity for phosphotyrosine peptides. Finally, the imprinted hydrogels were applied to specifically adsorb phosphotyrosine peptides from a sample mixture containing phosphotyrosine and a tryptic digest of β‐casein, which demonstrated high selectivity. After four rebinding cycles, 78.9% adsorption efficiency was still retained.     

L-酪氨酸印迹分子的制备及性能研究

利用分子印迹技术采用传统加热法制备出酪氨酸他子印迹聚合物。用红外光谱分析了聚合物结构。研究了印迹他子与功能单体的物质的量对聚合物结合性的影响,吸收效率表征结果显示,与化学组成相同的空白聚合物相比,印迹聚合物具有更高的吸附效率。     

氨基酸衍生物手性分离分子印迹聚合物

本文从识别机理,制备方法及其应用等方面简要介绍了近年来用于氨基酸衍生物手性分离的分子印迹聚合物的一些研究进展.     

纳米结构分子印迹聚合物及其在药物分析中的应用进展

纳米材料是纳米技术发展的重要基础,它具有许多传统材料所不具备的独特的理化性质,因此有着广泛的应用前景.分子印迹技术是一种通过模拟抗体-抗原相互作用原理,制备具有分子识别功能的聚合物的技术.以纳米材料制备的分子印迹聚合物具有较高的结合容量,较大的选择性和较快的结合动力学特性,近年来备受关注.本文简单概述了零维、一维、二维纳米结构分子印迹聚合物的合成、表征方法及研究现状,并对其在手性药物分析、临床药物分析、传感器及药物残留检测中的应用进行了综述.