共查询到20条相似文献,搜索用时 15 毫秒
1.
A Functionalized Hollow Mesoporous Silica Nanoparticles‐Based Controlled Dual‐Drug Delivery System for Improved Tumor Cell Cytotoxicity
下载免费PDF全文
![点击此处可从《Particle & Particle Systems Characterization》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yunfeng Jiao Shun Shen Yangfei Sun Xingguo Jiang Wuli Yang 《Particle & Particle Systems Characterization》2015,32(2):222-233
Multifunctional nanoparticles for selectively targeting tumor cells and effectively delivering multiple drugs are urgently needed in cancer therapy. Here, a dual‐drug delivery system is prepared, based on functionalized hollow mesoporous silica nanoparticles (HMSNs). Doxorubicin (DOX) hydrochloride is loaded into the hollow core, and dichloro(1,2‐diaminocyclohexane)platinum (II) (DACHPt) is stored in the pores of the shell by the coordination interaction with the carboxyl groups modified on the pore walls, which also serves as barriers to control the DOX release. Detailed studies in vitro indicate that the DACHPt release is triggered by Cl? through the cleavage of the coordination interaction, and the DOX release depends on the release rate of DACHPt and the environmental pH value. The surface of the mechanized nanoparticles is also modified by transferrin (Tf) to achieve the tumor specificity. Compared with individual drug delivery systems, the dual‐drug delivery system shows synergistic efficacy on the cell cytotoxicity (combination index = 0.30), resulting in improved tumor cell killing. The present dual‐drug delivery system provides a promising strategy to develop controlled and targeted combination therapies for efficient cancer treatment. 相似文献
2.
Krishna Radhakrishnan Satyajit Gupta Divya Prakash Gnanadhas Praveen C. Ramamurthy Dipshika Chakravortty Ashok M. Raichur 《Particle & Particle Systems Characterization》2014,31(4):449-458
The fabrication of a mesoporous silica nanoparticle (MSN)?protamine hybrid system (MSN?PRM) is reported that selectively releases drugs in the presence of specific enzyme triggers present in the proximity of cancer cells. The enzyme trigger involved is a protease called trypsin, which is overexpressed in certain specific pathological conditions, such as inflammation and cancer. Overexpression of trypsin is known to be associated with invasion, metastasis, and growth in several cancers, such as leukemia, colon cancer, and colorectal cancer. The current system (MSN–PRM) consists of an MSN support in which mesopores are capped with an FDA‐approved peptide drug protamine, which effectively blocks the outward diffusion of the drug molecules from the mesopores of the MSNs. On exposure to the enzyme trigger, the protamine cap disintegrates, opening up the molecular gates and releasing the entrapped drug molecules. The system exhibits minimal premature release in the absence of the trigger and selectively releases the encapsulated drugs in the presence of the proteases secreted by colorectal cancer cells. The ability of the MSN–PRM particles to deliver anticancer drugs to colorectal cancer cells has also been demonstrated. The hydrophobic drug is released into cancer cells subsequent to disintegration of the protamine cap, resulting in cell death. Drug‐induced cell death in colorectal cancer cells is significantly enhanced when the hydrophobic drug that is known to degrade in aqueous environments is encapsulated in the MSN–PRM system in comparison to the free drug (P < 0.05). The system, which shows good biocompatibility and selective drug release, is a promising platform for cancer specific drug delivery. 相似文献
3.
Madhappan Santha Moorthy Jae‐Ho Bae Mi‐Ju Kim Sun‐Hee Kim Chang‐Sik Ha 《Particle & Particle Systems Characterization》2013,30(12):1044-1055
The integration of unique functionality into mesoporous organosilica hybrid carriers is an important issue in solving the challenges of dual/multi delivery for combined therapy with drugs with a distinct therapeutic effects. Newly designed mesoporous organosilica hybrid microcarriers (HMCs) are synthesized on the basis of the triblock‐copolymer‐templated sol–gel method. The synthesized HMCs, which integrate both heteroaromatic pyridine and diurea functionalities, are combined in a mesoporous organosilica hybrid network to design functional hybrid microcarriers with a range of mechanisms for the pH‐triggered release of two drugs. The drugs include the hydrophilic anticancer therapeutic agent 5‐fluorouracil (5‐FU) and the non‐steroidal hydrophobic anti‐inflammatory drug ibuprofen (IBU). 5‐FU and IBU are encapsulated in the HMCs using multiple hydrogen bonding and electrostatic interaction sites and are delivered under a range of pH conditions. The release of 5‐FU and IBU is tested at pH 5.5 and 7.4. The results show that the release is sensitive to pH. The antitumor activity of the released 5‐FU is evaluated using the MCF‐7 cell line. The released 5‐FU has the capacity to kill cancer cells under acidic pH conditions. 相似文献
4.
5.
Glutathione‐Mediated Degradation of Surface‐Capped MnO2 for Drug Release from Mesoporous Silica Nanoparticles to Cancer Cells
下载免费PDF全文
![点击此处可从《Particle & Particle Systems Characterization》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Xiaoxiao He Kemin Wang Zhen Zou Xuecai Li Hui Shi Jierong Luo Xiaoxiao Yang 《Particle & Particle Systems Characterization》2015,32(2):205-212
Owing to its higher concentration in cancer cells than that in the corresponding normal cells, glutathione (GSH) provides an effective and flexible mechanism to design drug delivery systems. Here a novel GSH‐responsive mesoporous silica nanoparticle (MSN) is reported for controlled drug release. In this system, manganese dioxide (MnO2) nanostructure, formed by the reduction of KMnO4 on the surface of carboxyl‐functionalized MSN can block the pores (MSN@MnO2). By a redox reaction, the capped MnO2 nanostructure can dissociate into Mn2+ in the presence of GSH molecules. The blocked pores are then uncapped, which result in the release of the entrapped drugs. As a proof‐of‐concept, doxorubicin (DOX) as model drug is loaded into MSN@MnO2. DOX‐loaded MSN@MnO2 shows an obvious drug release in 10 × 10?3 m GSH, while no release is observed in the absence of GSH. In vitro studies using human hepatocellular liver carcinoma cell line (HepG2) prove that the DOX‐loaded MSN@MnO2 can entry into HepG2 cells and efficiently release the loaded DOX, leading to higher cytotoxicity than to that of human normal liver cells (L02). It is believed that further developments of this GSH‐responsive drug delivery system will lead to a new generation of nanodevices for intracellular controlled delivery. 相似文献
6.
Chaohui Zhou Hui Wu Chusen Huang Mingliang Wang Nengqin Jia 《Particle & Particle Systems Characterization》2014,31(6):675-684
In this work, a new and facile strategy is developed to synthesize a single‐phase Eu3+‐doped mesoporous gadolinium oxide nanorods (MS‐Gd2O3:Eu@PEG) by incorporating a facile wet‐chemical route, which includes an induced silica layer being coated onto the nanorods, and evolution of pores and formation of channels, as well as a surface‐modified process for multimodal imaging and anti‐cancer drug delivery. The properties of these as‐prepared Gd2O3:Eu nanorods are characterized by transmission electron microscopy (TEM), X‐ray diffraction (XRD), N2 adsorption/desorption, and photoluminescence (PL). The in vitro cytotoxicity test, drug loading, and drug release experiments reveal that the MS‐Gd2O3:Eu@PEG nanorods have good biocompatibility, efficient loading capacity, and pH‐sensitive releasing behavior, suggesting the nanorods could be an ideal candidate as drug delivery vehicles for cancer therapy. Furthermore, the MS‐Gd2O3:Eu@PEG nanorods show clearly dose‐dependent contrast enhancement in T1‐weighted magnetic resonance images and can potentially be used as a T1‐positive contrast agent. These results indicate our prepared multifunctional mesoporous gadolinium oxide nanorods can serve as a promising platform for simultaneous anti‐cancer drug delivery and multimodal imaging. 相似文献
7.
Human‐Serum‐Albumin‐Coated Prussian Blue Nanoparticles as pH‐/Thermotriggered Drug‐Delivery Vehicles for Cancer Thermochemotherapy
下载免费PDF全文
![点击此处可从《Particle & Particle Systems Characterization》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Zhenglin Li Ying Hu Tingting Jiang Kenneth A. Howard Yonggang Li Xuelei Fan Ye Sun Flemming Besenbacher Miao Yu 《Particle & Particle Systems Characterization》2016,33(1):53-62
Constructing novel multimodal antitumor therapeutic nanoagents has attracted tremendous recent attention. In this work, a new drug‐delivery vehicle based on human‐serum‐albumin (HSA)‐coated Prussian blue nanoparticles (PB NPs) is synthesized. It is demonstrated that doxorubicin (DOX)/HSA is successfully loaded after in situ polymerization of dopamine onto PB NPs, and the PB@PDA/DOX/HSA NPs are highly compatible and stable in various physiological solutions. The NPs possess strong near‐infrared (NIR) absorbance, and excellent capability and stability of photothermal conversion for highly efficient photothermal therapy applications. Furthermore, a bimodal on‐demand drug release sensitively triggered by pH or NIR irradiation has been realized, resulting in a significant chemotherapeutic effect due to the preferential uptake and internalization of the NPs by cancer cells. Importantly, the thermochemotherapy efficacy of the NPs has been examined by a cell viability assay, revealing a remarkably superior synergistic anticancer effect over either monotherapy. Such multifunctional drug‐delivery systems composed of approved materials may have promising biomedical applications for antitumor therapy. 相似文献
8.
Near‐Infrared‐Light‐Induced Fast Drug Release Platform: Mesoporous Silica‐Coated Gold Nanoframes for Thermochemotherapy
下载免费PDF全文
![点击此处可从《Particle & Particle Systems Characterization》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Haosha Tang Liangqing Yao Jianping Yang Wei Li Zhaogang Teng Congjian Xu 《Particle & Particle Systems Characterization》2016,33(6):316-322
Development of advanced theranostics for personalized medicine is of great interest. Herein, a multifunctional mesoporous silica‐based drug delivery carrier has been developed for efficient chemo/photothermal therapy. The unique Au nanoframes@mSiO2 spheres are elaborately prepared by utilizing Ag@mSiO2 yolk–shell spheres as the template through spatially confined galvanic replacement method. Compared with the Ag@mSiO2 yolk–shell spheres, the resultant Au nanoframes@mSiO2 spheres show a strong and broad near‐infrared (NIR) absorbance in the 550–1100 nm region, high surface areas, and good biocompatibility. When irradiated with a NIR laser with a power intensity of 1 W cm?2 at 808 nm, they can become highly localized heat sources through the photothermal effect. Moreover, the photothermal effect of the Au nanoframes can significantly promote the fast release of doxorubicin. The in vitro studies show obvious synergistic effects combining photothermal therapy and chemotherapy in the Au nanoframes@mSiO2 spheres against Hela cells. It is believed that the as‐obtained multifunctional vehicles provide a promising platform for the combination of hyperthermia and chemotherapy for cancer treatment application. 相似文献
9.
Feng Lu Chen Zhan Yi Gong Yufu Tang Chen Xie Qi Wang Wenjun Wang Quli Fan Wei Huang 《Particle & Particle Systems Characterization》2020,37(6):1900483
Although semiconducting polymers (SPs) have become an important category for optical imaging and phototherapy, their biomedical application is still facing a number of challenges. Herein, a cationic surfactant–assisted approach to encapsulate hydrophobic SPs within highly PEGylated mesoporous silica (mSiO2) nanoparticles with excellent colloidal stability and enhanced fluorescence in aqueous solution is reported. In comparison to the previously reported amphiphilic polymer coating and silification method, this universal strategy not only suppresses the formation of empty polymer micelles and free silica nanoparticles, but also provides high specific surface area for drug loading. As a proof of concept, furan-containing diketopyrrolopyrrole-based semiconducting polymers (PDFT) are coated with mesoporous silica and utilized for fluorescence imaging in the second near-infrared region (NIR-II, 1000–1700 nm) and drug delivery. In vivo blood vessel imaging and tumor imaging are achieved with high resolution (0.21 mm) and signal-to-background ratio (≈4.2). Additionally, pH-responsive drug release and improved therapeutic effect are observed. By choosing desired SPs, different optical imaging and therapeutic modalities can also be achieved, thus the SP@mSiO2 nanostructures obtained here provide numerous opportunities for theranostic applications. 相似文献
10.
An acid‐labile doxorubicin dimer (D‐DOX) is designed as drug–drug conjugate for tumor intracellular pH‐triggered release, by conjugating doxorubicin (DOX) with adipic acid dihydrazide (ADH). The dimer‐based surfactants modified with polyethylene glycol (PEG), DOX‐ADH‐DOX‐PEG or are synthesized by mono‐PEGylation and bi‐PEGylation, respectively. Then the prodrug nanoparticles are fabricated with different drug contents via dialyzing the mixture solution of D‐DOX and the PEGylated surfactants in dimethyl sulfoxide (DMSO) with different mass ratios against water. It is found that the smaller prodrug nanoparticles (142–163 nm) could be obtained with the mono‐PEGylated surfactant, than those of 157–225 nm with the bi‐PEGylated surfactant. Furthermore, the mono‐PEGylated surfactant results in a higher drug content of 51% due to their lower PEG contents. All prodrug nanoparticles could release DOX completely within 36 h at pH 5.0, with the premature drug leakage of less than 10% at pH 7.4. The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assays demonstrate the proposed drug self‐delivery system possessed an enhanced anticancer efficacy against HepG2 cells than the free DOX. 相似文献
11.
12.
Temperature and Redox Dual‐Responsive Biodegradable Nanogels for Optimizing Antitumor Drug Delivery
下载免费PDF全文
![点击此处可从《Particle & Particle Systems Characterization》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yefei Tian Yang Wang Shun Shen Xinguo Jiang Yajun Wang Wuli Yang 《Particle & Particle Systems Characterization》2015,32(12):1092-1101
The strategy to efficiently deliver antitumor drugs via nanocarriers to targeted tumor sites and achieve controllable drug release is attracting great research interest in cancer therapy. In this study, a novel type of disulfide‐bonded poly(vinylcaprolactam) (PVCL)‐based nanogels with tunable volume phase transition temperature and excellent redox‐labile property are prepared. The nanogels are hydrophilic and swell at 37 °C, whereas under hyperthermia (e.g., 41 °C), the nanogels undergo sharp hydrophilic/hydrophobic transition and volume collapse, which enhances the cellular uptake and drug release. The incorporation of disulfide bond linkers endows the nanogels with an excellent disassembly property in reducing environments, which greatly facilitates drug release in tumor cells. Nanogels loaded with doxorubicin (DOX) (DOX‐NGs) (DOX‐NGs) are stable in physiological conditions with low drug leakage (15% in 48 h), while burst release of DOX (92% in 12 h) can be achieved in the presence of 10 × 10?3 m glutathione and under hyperthermia. The DOX‐NGs possess improved cell killing efficiency under hyperthermia (IC50 decreased from 1.58 μg mL?1 under normothermia to 0.5 μg mL?1). Further, the DOX‐NGs show a pronounced tumor inhibition rate of 46.6% compared with free DOX, demonstrating that this new dual‐responsive nanogels have great potential as drug delivery carriers for cancer therapy in vivo. 相似文献
13.
Poly(N‐vinylcaprolactam)‐based biodegradable microgels are prepared for drug delivery application via precipitation polymerization using diacetone acrylamide (DAAM) and dimethyl itaconate (IADME) as comonomers. The microgel particles are subsequently crosslinked by addition of adipic acid dihydrazide, which reacts with the ketone groups of DAAM. Itaconic acid (IA) groups are generated by the hydrolysis of IADME units inside the microgels resulting into both pH and temperature sensitive microgel particles. Volume phase transition temperature of the obtained microgels is influenced by both IA content and pH of the surrounding medium. Due to the incorporation of hydrazone linkages, the microgels show degradation under acidic conditions. These microgels can effectively encapsulate doxorubicin (DOX) as a model drug and show low DOX leakage under physiological conditions while rapid DOX release is observed at low pH. The results of the cytotoxicity assay further display that the DOX‐loaded microgels exhibit effective antitumor activity against HeLa cells demonstrating their great potential as drug delivery carriers for cancer therapy. 相似文献
14.
Hctor Lpez‐Laguna Rita Sala Julieta M. Snchez Patricia lamo Ugutz Unzueta Alejandro Snchez‐Chardi Naroa Serna Laura Snchez‐García Eric Volt‐Durn Ramn Mangues Antonio Villaverde Esther Vzquez 《Particle & Particle Systems Characterization》2019,36(11)
Cell‐selective targeting is expected to enhance effectiveness and minimize side effects of cytotoxic agents. Functionalization of drugs or drug nanoconjugates with specific cell ligands allows receptor‐mediated selective cell delivery. However, it is unclear whether the incorporation of an efficient ligand into a drug vehicle is sufficient to ensure proper biodistribution upon systemic administration, and also at which extent biophysical properties of the vehicle may contribute to the accumulation in target tissues during active targeting. To approach this issue, structural robustness of self‐assembling, protein‐only nanoparticles targeted to the tumoral marker CXCR4 is compromised by reducing the number of histidine residues (from six to five) in a histidine‐based architectonic tag. Thus, the structure of the resulting nanoparticles, but not of building blocks, is weakened. Upon intravenous injection in animal models of human CXCR4+ colorectal cancer, the administered material loses the ability to accumulate in tumor tissue, where it is only transiently found. It instead deposits in kidney and liver. Therefore, precise cell‐targeted delivery requires not only the incorporation of a proper ligand that promotes receptor‐mediated internalization, but also, unexpectedly, its maintenance of a stable multimeric nanostructure that ensures high ligand exposure and long residence time in tumor tissue. 相似文献
15.
Thomas L. Moore Joshua E. Pitzer Ramakrishna Podila Xiaojia Wang Robert L. Lewis Stuart W. Grimes James R. Wilson Even Skjervold Jared M. Brown Apparao Rao Frank Alexis 《Particle & Particle Systems Characterization》2013,30(4):365-373
Although progress in the use carbon nanotubes in medicine has been most encouraging for therapeutic and diagnostic applications, any translational success must involve overcoming the toxicological and surface functionalization challenges inherent in the use of such nanotubes. Ideally, a carbon‐nanotube‐based drug delivery system would exhibit low toxicity, sustained drug release, and persist in circulation without aggregation. Here, carbon nanotubes (CNTs) coated with a biocompatible block‐co‐polymer composed of poly(lactide)‐poly(ethylene glycol) (PLA‐PEG) are reported to reduce short‐term and long‐term toxicity, sustain drug release of paclitaxel (PTX), and prevent aggregation. The copolymer coating on the surface of CNTs significantly reduces in vitro toxicity. Moreover, the coating reduces the in vitro inflammatory response. Compared to non‐coated CNTs, in vivo studies show no long‐term inflammatory response with CNT coated with PLA‐PEG (CLP) and the surface coating significantly decreases acute toxicity by doubling the maximum tolerated dose in mice. In vivo biodistribution and histology studies suggest a lower degree of aggregation in tissues. 相似文献
16.
Self‐nanoemulsifying drug delivery system (SNEDDS) is recently studied for enhancing the bioavailability of hydrophobic drugs, such as resveratrol (RSV). However, the functional design of SNEDDS for the drug structural protection is not studied yet. Here, this paper presents an efficient approach by adding lignin (a kind of abundant biomass resource) to enhance the stability of trans‐RSV and meantime improve its oral bioavailability. Higher stability of trans‐RSV (just decreased 2% to cis‐RSV after 1 h under direct sunlight) is observed after loaded by lignin‐containing SNEDDS (RSV/SLS SNEDDS). Low toxicity, high transfer efficiency (apparent permeability coefficient >1 × 10−7 cm s−1), and bioavailability (1.371%) of RSV/SLS SNEDDS is successfully demonstrated in Caco‐2 cells and rat model, respectively. This study serves to illustrate a simple, versatile, environmental, and economic sustainability approach to the development of nanodelivery systems for light instability drugs. 相似文献
17.
Thermo‐chemotherapy combining photothermal therapy (PTT) with chemotherapy has become a potent approach for antitumor treatment. In this study, a multifunctional drug‐delivery nanoplatform based on polyethylene glycol (PEG)‐modified mesoporous silica‐coated bismuth selenide nanoparticles (referred to as Bi2Se3@mSiO2‐PEG NPs) is developed for synergistic PTT and chemotherapy with infrared thermal (IRT) imaging of cancer cells. The product shows no/low cytotoxicity, strong near‐infrared (NIR) optical absorption, high photothermal conversion capacity, and stability. Utilizing the prominent photothermal effect, high‐contrast IRT imaging and efficient photothermal killing effect on cancer cells are achieved upon NIR laser irradiation. Moreover, the successful mesoporous silica coating of the Bi2Se3@mSiO2‐PEG NPs cannot only largely improve the stability but also endow the NPs high drug loading capacity. As a proof‐of‐concept model, doxorubicin (DOX) is successfully loaded into the NPs with rather high loading capacity (≈50.0%) via the nanoprecipitation method. It is found that the DOX‐loaded NPs exhibit a bimodal on‐demand pH‐ and NIR‐responsive drug release property, and can realize effective intracellular drug delivery for chemotherapy. The synergistic thermo‐chemotherapy results in a significantly higher antitumor efficacy than either PTT or chemotherapy alone. The work reveals the great potential of such core–shell NPs as a multifunctional drug‐delivery nanosystem for thermo‐chemotherapy. 相似文献
18.
As a kind of natural protein, keratin is widely investigated in the biomedical field. Here, for the first time, a keratin‐based prodrug (PK‐SS‐D) is designed for tumor intracellular reduction triggered drug delivery, by conjugating doxorubicin (DOX) onto poly(ethylene glycol) modified keratin (PEGylated keratin, PK) with a bioreducible disulfide linkage. The protein‐drug conjugate prodrug, with a drug content of 20%, can self‐assemble into micelles with a mean hydrodynamic diameter of 175 nm and a narrow distribution. The in vitro controlled release profiles reveal the reduction triggered thiolated DOX (DOX‐SH) release behavior of the PK‐SS‐D micelles, with a cumulative drug release up to 52% within 10 d in the simulated tumor microenvironment in a sustained releasing mode, and a low drug leakage of 17% in the simulated normal physiological medium. The enhanced tumor growth inhibition of the proposed PK‐SS‐D prodrug micelles is revealed by the methyl tetrazolium (MTT) assays, although the released DOX‐SH prodrug possesses a lower tumor growth inhibition than DOX. 相似文献
19.
Hyunsik Choi Byung Woo Hwang Kyeng Min Park Ki Su Kim Sei Kwang Hahn 《Particle & Particle Systems Characterization》2020,37(1):1900418
Recently, micro/nanomotor systems have been widely investigated for biomedical applications especially for the active transport and delivery of specific drugs. However, there are few stimuli-responsive micro/nanomotor systems to enhance the drug delivery efficiency and reduce side effects by the spatiotemporal controllability. Here, a degradable nanomotor is first fabricated for targeted drug delivery using a platinum (Pt)-deposited complex of calcium carbonate and cuccurbit[6]uril-conjugated hyaluronate (Pt/CaCO3@HA-CB[6]). The nanomotors could efficiently deliver model drugs to the cells in reactive oxygen species (ROS) abundant environments such as the tumor site. After reaching the tumor site around pH 6.5, Pt/CaCO3@HA-CB[6] nanomotors (≈1 µm) are pH-responsively disintegrated by the dissociation of CaCO3 and the encapsulated HA-CB[6] (≈300 nm) are released for cancer cell uptake. The released HA conjugate are finally uptaken into cancer cells via HA receptor-mediated endocytosis. Moreover, model drugs are modularly loaded into the nanomotors via the host–guest chemistry of CB[6] for stable delivery to cancer cells. Taken together, Pt/CaCO3@HA-CB[6] nanomotors systems could be successfully harnessed for active drug delivery to cancer cells. 相似文献
20.
Theranostics: Magnetic Liposomal Particles for Magnetic Imaging,Sensing, and the pH‐Sensitive Delivery of Therapeutics (Part. Part. Syst. Charact. 5/2016)
下载免费PDF全文
![点击此处可从《Particle & Particle Systems Characterization》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Yong‐Su Kwon Su Jin Jang Young Il Yoon Hee‐Sang Cho Hak Jong Lee Young‐Seok Cho Hyun Soo Shin Tae‐Jong Yoon 《Particle & Particle Systems Characterization》2016,33(5):237-237