电化学法制备氧化亚铜的研究

采用电化学法制备氧化亚铜 .研究了电解液组成及其浓度、温度以及电流密度等因素对氧化亚铜产品质量的影响 ,选定的优化工艺条件 :ρ(NaCl) =2 6 0～ 2 90g/L ,ρ(NaOH) =0 .5 0g/L(pH =12 .1) ;电解温度为 75～ 80℃ ;电流密度为 75 0A/m2     

电化学法制备石墨烯及其导电特性

采用电化学方法将石墨层电解剥离, 得到分散于电解质溶液的结构较为完整的石墨烯. 用透射电子显微镜和拉曼光谱分析了石墨烯的形貌和结构, 利用四探针法测定了石墨烯导电特性. 实验数据和理论拟合结果表明, 当100 K相似文献   

青翘清热解毒活性组分及成分筛选

探讨青翘清热解毒活性组分。以C_(18)为分离材料将青翘60%乙醇提取物分离为6个化学成分群F_1～F_6;以清除DPPH·的能力、抑菌效果和改善LPS诱导的小鼠巨噬细胞RAW264.7产生炎症反应的程度为评价指标,评价不同化学成分群的抗氧化、抑菌和抗炎活性。F_2化学成分群在抗氧化、抑菌和抗炎活性等方面均具有较强的活性;进一步化学成分分析表明青翘清热解毒活性组分F_2主要是由苯乙醇苷类成分组成,主要成分为连翘酯苷A和连翘酯苷I。青翘清热解毒活性活性成分主要为苯乙醇苷类成分。     

电化学法合成强荧光CdTe量子点

采用电化学方法产生的H2Te为碲源(Te2-),快速合成了水溶性强荧光的CdTe量子点.该方法具有操作简单、安全、快速廉价和可大量制备等优点.合成过程中考察了合成温度,pH值和配体比例对制备CdTe量子点的影响.在最优化的实验条件下,电化学方法合成的巯基丙酸配位的CdTe荧光量子产率可达到55％;通过紫外可见光谱(UV...     

甲基苯丙胺电化学检测条件的优化

采用电化学方法检测甲基苯丙胺,探讨了不同扫描速度、初始浓度、pH值、电极处理温度及干扰物对检测的影响,获得了甲基苯丙胺电化学检测的最佳条件.结果表明,工作电极为掺硼金刚石薄膜电极,扫描速度为50~100 m V/s,初始浓度小于100 mg/L,pH=7.0及电极处理温度为100℃时,甲基苯丙胺的检测速度最快,检测结果最好,同时干扰物对于电化学方法检测甲基苯丙胺无明显影响.     

电化学法提锂技术的研究进展及展望

随着新能源产业的不断发展,锂资源的需求量急剧增加。我国具有丰富盐湖锂资源,随着技术的革新与产业化的实践,盐湖提锂技术逐渐走向成熟。电化学法提锂作为众多提锂技术中的新秀,因绿色环保、选择性高、低能耗而备受国内外研究者的关注。本文对国内外电化学提锂技术的研究进展进行综述,并对未来电化学提锂技术的发展给出建议与展望。     

分子生物色谱用于中药活性成分筛选及质量控制方法的研究

报道了近期工作进展,首先阐述了分子生物色谱的基本原理及特点,然后介绍了分子生物色谱对多种中药、不同产地的同种中药活性成分谱图模式的比较,结合已有的工作对活性成分筛选方法、相互作用研究、质量控制方法发展做了细致的说明,并讨论了其发展方向及前景。     

电化学法研究蛋白质和茜素红S的相互作用

在pH 4 .2的Britton Robinson缓冲液中 ,茜素红S(ARS)与牛血清白蛋白 (BSA)能形成一种红色的非电活性的超分子复合物。用线性扫描二阶导数极谱法和循环伏安法对该体系进行了研究 ,复合物的形成使ARS的还原峰电流下降 ,峰电流的下降值同所加的BSA浓度在一定范围内呈线性关系。用于BSA的测定 ,在 8.0× 10 -8～ 1.2× 10 -6mol/L范围内呈线性关系 ,检测限为 4 .3× 10 -8mol/L ,对结合反应机理进行初步的探讨     

中草药活性成分的高通量筛选技术研究进展

随着高通量筛选技术的不断发展,该技术已经成为发现新药物的重要途径之一。高通量筛选技术已大量应用于筛选药物活性成分的领域中,但是其中大部分为从化合物库中筛选活性成分,仅有十几篇文献应用于中药活性成分的筛选,而中国传统中草药却是探索和发展新药物的丰富来源。本文通过综述国内外2008年到2017年的相关文献,阐述了分子和细胞水平上的高通量筛选技术在中草药活性成分的筛选及其应用进展,为今后中草药新药研发提供参考。     

部分电化学还原氧化石墨烯修饰玻碳电极用于苦参碱的测定

以部分电化学还原的氧化石墨烯(pErGO)修饰的玻碳电极(GCE)作为工作电极(pErGO/GCE),用于苦参碱(MT)含量的电化学测定.在活化好的GCE上滴涂氧化石墨烯(GO),用恒电位法在-0.75 V下还原GCE表面的GO 200 s,得到的电极即为pErGO/GCE.以0.1 mol·L-1 NaH2 PO4-...     

毛细管电泳-柱端电化学检测池的设计

在射壁式检测器的基规上,设计了一种用于毛细管电泳-电化学检测装置的检测池,巧妙地利用因表面张力现象而留驻波滴曲面的放大作用,直接调整毛细管与电极之间的相对位置,无需显微镜-微调节器装置,具有空间体积小,操作更简便的优点。     

鸡肉中多种磺胺兽药残留量测定的高效液相色谱-电化学检测法

采用液相色谱电化学法测定了鸡肉中磺胺类药物残留量；磺胺用氯仿提取后，取部分提取液用氮气吹干，残渣溶于KH2PO4中，用正己烷脱脂，水相进样，液相色谱分析用C18柱，流动相为甲醇－0．01mol/L KH2PO4(pH6，体积比25：75），检测电位1．0V；与紫外检测器相比，电化学检测器（ECD）有更高的灵敏度和选择性，ECD的检出限为磺胺嘧啶0.02ng，磺胺甲氧哒嗪0．06ng，磺胺甲基异恶唑0.07ng。     

高效液相色谱-脉冲积分安培电化学检测牛奶中的妥布霉素

采用C18键合硅胶柱和脉冲积分安培电化学检测器对妥布霉素样品进行了分离检测。对两种检测电位波形进行了对比，并选取了表现优良的检测电位。方法对妥布霉素的检出限19μg／L，在0．1～25mg／L范围内表现良好线性（r=0．9999）。该方法应用于市售鲜奶的检测，妥布霉素的回收率为98％。     

Acute Toxicity Assay Based on Electrochemical Method for Detection of Antibiotic Residues in Water

In this work, the bacteria suspended in solution or immobilized in the biofilm were employed to study whether bacterial electrochemistry can reflect the impacts of the antibiotic residues in water on microorganisms. In the sensing system with immobilized bacteria, the tested antibiotics of 10∼40 mg/L showed an opportunity to inhibit respiration over a short time and the combined effect of these antibiotics exhibited a complex dose-dependence. The resistance was induced for the immobilized bacteria in the repeated measurement over a long-term period, which was fortunately solved by refreshing the biofilm via a nutrient supplementation. In the solution system, bacterial respirations were promoted by the tested antibiotics even at concentrations up to 160 mg/L, but β-glucosidase activity was inhibited by only 0.05 mg/L antibiotic. Cu²⁺ and Zn²⁺ were further inducted in the suspended system, and the combination of metals and antibiotics showed an obvious antagonistic effect. Real water detection indicated that the bacterial electrochemical method was expected to warn the water qualities with a sudden change by an appropriate testing condition.     

A Facile Graphene Nanosheets‐based Electrochemical Sensor for Sensitive Detection of Honokiol in Traditional Chinese Medicine

The sensitive detection of honokiol was performed using a graphene nanosheets‐based electrochemical sensor by cyclic voltammogram and a differential pulse anodic stripping voltammogram. Several important parameters such as deposition cycle of reduced graphene oxide, the acidity of the running buffer, accumulation potential and accumulation time were investigated to acquire the optimum conditions. The sensor was further applied to quantification of honokiol in the concentration range from 0.005 to 10 µM with a low detection limit of 1.7 nM. Finally, the sensor successfully determined the content of honokiol in Ageratum Liquid with a satisfied recovery of 98.2 %～99.1 %.     

毛细管电泳-电化学检测法测定蜘蛛香中多元酚类化合物

采用毛细管电泳 电化学检测法(CE ED)同时测定了蜘蛛香根中香叶木素、山奈酚、芹菜素、绿原酸 和咖啡酸等5种主要生物活性成分的含量,考察了运行缓冲液酸度、浓度、分离电压、氧化电位和进样时间等 实验参数对分离、检测的影响。在最佳实验条件下,以直径300μm的碳圆盘电极为工作电极,检测电位为+ 950mV(vs.SCE),在50mmol/L的硼砂缓冲溶液(pH9.23)中,上述各组分在23min内能完全分离。5种组 分在两个数量级的范围内呈良好线性关系,检测下限(S/N=3)达1.7×10-4～1.8×10-5g/mL。该法已成功 地应用于蜘蛛香根中活性成分的分离检测,结果令人满意。     

基于电化学传感器检测赭曲霉毒素A的研究进展

赭曲霉毒素A(Ochratoxin A,OTA)是一种由赭曲霉、青霉菌等真菌产生的次级代谢产物,在自然界分布广泛,具有肝毒性、肾毒性、致癌、致突变作用.鉴于其危害的严重性和污染的广泛性,发展高灵敏度的OTA检测技术引起了研究者的广泛关注.该文阐述了近年来新型电化学传感技术在OTA检测方面的发展及应用,全面综述了目前OT...     

纳米材料电化学与生物传感器——有机微污染物检测新途径

本文介绍了近年来纳米材料电化学与生物传感器在有机微污染物检测中的研究现状,分析了这些传感器中纳米材料修饰电极的特点,重点阐述了纳米材料在有机微污染物检测中的重要作用,列举了一些纳米材料电化学与生物传感器在有机微污染物检测中的应用。最后对纳米材料电化学与生物传感器用于有机微污染物的检测研究进行了简要评述和展望。     

Amperometric Tyrosinase Biosensor Based on Carbon Black Paste Electrode for Sensitive Detection of Catechol in Environmental Samples

In this work, a renewable tyrosinase-based biosensor was developed for the detection of catechol, using a carbon black paste electrode, without any mediator. The effect of pH, type of electrolyte, and amount of tyrosinase enzyme were explored for optimum analytical performance. The best-performing biosensor in amperometric experiments at potential −0.2 V vs. Ag/AgCl (3 mol L⁻¹ KCl) was obtained using a 0.1 mol L⁻¹ phosphate buffer solution (pH 7.0) as electrolyte. Under optimized conditions, the proposed biosensor had two concentration linear ranges from 5.0×10⁻⁹ to 4.8×10⁻⁸ and from 4.8×10⁻⁸ to 8.5×10⁻⁶ mol L⁻¹ and a limit of detection of 1.5×10⁻⁹ mol L⁻¹. The apparent Michaelis-Menten constant ( ) was calculated by the amperometric method, and the obtained value was 1.2×10⁻⁵ mol L⁻¹ whose result was similar when compared with other studies previously. The biosensor was applied in river water samples, and the results were very satisfactory, with recoveries near 100 %. In addition, the response of this biosensor for different compounds, taking into account their molecular structures was investigated and the results obtained showed no interference with the response potential of catechol. The electrochemical biosensor developed in this work can be considered highly advantageous because it does not require the use of a mediator (direct detection) for electrochemical response, and also because it is based on a low-cost materials that can be used with success to immobilise other enzymes and/or biomolecules.