敌鼠钠盐—TritonX—100荧光光度法测定铕

敌鼠是一种广泛应用的高效抗凝血类杀鼠剂,其化学名称为2-(2,2-二苯基乙酰基)-1,3-茚满二酮,结构式为:     

Study of the Fluorescence System of Europium–Enoxacin and its Applications

 This paper is the study of the fluorescence enhancement of Eu³⁺-1-ethyl-6-fluoro-4-oxo-7-(1-piperazinyl)-1,8-naphthyridin-3-carbonic acid (enoxacin, EFLX) system by surfactants. It was found that sodium dodecylbenzenesulfonate (SDBS) exhibits great enhancement on the fluorescence of the Eu-EFLX system. The molar ratio is 1:2:1 for Eu:EFLX: SDBS. Under the optimum conditions, the fluorescence intensity is a linear function of europium in the range of 1.0 × 10⁻⁸ ∼ 5.0 × 10⁻⁶ mol/L, the detection limit is 1.0 × 10⁻⁹ mol/L. The application of the Eu-EFLX-SDBS system for the determination of trace europium in rare earth samples gave satisfactory results. Received October 19, 2000. Revision August 10, 2001.     

铕—敌鼠—DL—组氨酸—十六烷基三甲基溴化铵荧光体系及其应用

研究了铕－敌鼠－ＤＬ－组氨酸－ＣＴＭＡＢ荧光体系的荧光特性，测定了体系的影响因素和测定敌鼠的实验方法，体系的激发和发射波长分别为３３０ｎｍ和６１２ｎｍ，各组分的最佳浓度分别为１．０×１０＾－５ｍｏｌ／Ｌ，ＤＬ－组氨酸，１．０×１０＾－４ｍｏｌ／Ｌ，ＣＴＭＡＢ１．０×１０＾－５ｍｏｌ／Ｌ，ｐＨ为９．０，在此条件下，敌鼠浓度在６．０×１０＾－７～９．０×１０＾－５ｍｏｌ／Ｌ范围内同体系的荧光强度成线性     

A New Fluorimetric Reagent for Determination of Trace Amounts of Europium

ＩｎｔｒｏｄｕｃｔｉｏｎＴｈｅｃｈｅｍｉｃａｌｐｒｏｐｅｒｔｉｅｓｏｆｒａｒｅ ｅａｒｔｈｅｌｅｍｅｎｔｓａｒｅｖｅｒｙｓｉｍｉｌａｒ ,ｃｏｎｓｅｑｕｅｎｔｌｙ ,ｉｔｉｓｄｉｆｆｉｃｕｌｔｔｏｄｅｔｅｃｔａｎｉｎ ｄｉｖｉｄｕａｌｉｏｎｉｎｔｈｅｉｒｍｉｘｔｕｒｅｓｏｗｉｎｇｔｏｔｈｅｉｎｔｅｒｆｅｒｅｎｃｅｏｆｏｔｈｅｒｒａｒｅ ｅａｒｔｈｅｌｅｍｅｎｔｓ .Ｄｕｅｔｏａｈｉｇｈｅｒｓｅｎｓｉｔｉｖｉｔｙａｎｄｓｅｌｅｃｔｉｖｉｔｙ ,ｆｌｕｏｒｉｍｅｔｒｉｃｍｅｔｈｏｄｓｏｆｒａｒｅ ｅａｒｔｈｅｌ…     

Enhanced Luminescence of Europium by Gadolinium and Its Application

Abstract

The fluorescence enhancement of the Eu-dibenzoylmethane(DBM)- cetylpyridinium-chloride (CPC) system was studied. The fluorescence intensity of the system can be greatly increased by Gd³⁺ in the presence of triethanolamine(TEA). The optimum conditions for enhanced fluorescence of the Eu-Gd-DBM-CPC system were examined. It was a linear function of the Eu³⁺ concentration in the range of 1.0×10^?10～1.0×10^?8 mol dm^?3 under the optimized conditions. The detection limit was 2.6 × 10¹¹ mol dm^?3 in an aqueous solution at pH=7.5～9.5.

The Eu-Gd-DBM-CPC system was applied to the determination of europium in rare earth oxides with satisfactory results. The mechanism of enhanced fluorescence was discussed.     

Enhanced Fluorimetric Determination of Europium(III) With Thenoyltrifluoroacetone and 4,7-Diphenyl-1,10-Phenanthroline by Gadolinium(III)

Abstract

A sensitive fluorimetric method was developed for the determination of trace amount of europium in rare earth oxides. In the presence of gadolinium, the fluorescence of the europium complex with thenoyltrifluoroacetone and 4,7-diphenyl-1,10-phenanthroline was increased by a factor of about 2; the excitation and emission wavelengths were 346 and 613nm, respectively;the fluorescence was linear up to 1.59ng/ml of europium;and the detection limit was found to be 0.6pg/ml of europium. A general mechanism of fluorescence enhancement was proposed.     

铕的示波极谱测定及在发光材料中的应用

本文研究了铕（Ⅲ）在乙二酸双（α─氨基乙基）醚四乙酸（简称ＧＢＥＴＡ）－ＮａＣｌ介质中的极谱行为及测定最佳条件，ＧＢＥＴＡ浓度为０．０１ｍｏｌ／Ｌ，ＮａＣｌ浓度为０．４ｍｏｌ／Ｌ，通Ｎ２除Ｏ２５ｍｉｎ。最低检出限为４．２×１０－７ｍｏｌ／Ｌ，测定了发光材料ＢａＦＣｌ（Ｅｕ）中Ｅｕ（Ⅱ）的含量。     

萘啶酮酸体系荧光法测定铕,铽,镝

本文研究了萘啶酮酸体系荧 光法同时测定铕、铽、镝的最佳条件。     

基于内滤效应的YVO4∶Eu荧光纳米探针测定色氨酸

采用溶剂热法合成了发光性能和水溶性良好的YVO4∶Eu纳米探针(YVO4∶Eu NPs).由于YVO4∶Eu NPs的激发光谱与色氨酸的吸收光谱有很大程度的重叠,二者可发生荧光内滤效应,其中色氨酸为吸光体、YVO4∶Eu NPs为荧光体,YVO4∶Eu NPs的发光被猝灭.基于此,建立了基于YVO4∶Eu NPs内滤效应测定色氨酸含量的方法.对YVO4∶Eu NPs的加入量、反应溶液pH值和反应时间进行了优化,在最佳反应条件下,本方法测定色氨酸的线性范围为4.0×10-6~4.0×10-4 mol/L,检出限为1.0 ×10-6 mol/L(3σ).采用本方法测定了酱油中色氨酸的含量,回收率为95.2%和97.3%.本方法具有简便快速、灵敏准确的特点.     

A new Fluorimetric Reagent for Highly Selective and Sensitive Determination of Terbium Ion

 In this work, a new ligand, 2,9-bis[N,N-bis(carboxymethyl)-aminomethyl]-1,10-phenanthroline (BBCAP), was synthesized and used to establish a novel fluorimetric method for the determination of trace amounts of terbium in a binary system. Its luminescence mechanism was studied. The excitation and emission wavelengths are 284 nm and 547 nm, respectively. Other rare-earth metal ions in 100-fold excess caused no interference. The fluorescence intensity was a linear function of the concentration of terbium in the range of 5.0 × 10⁻¹⁰–1.0 × 10⁻⁶ mol/L. The detection limit was 2.0 × 10⁻¹¹ mol/L (n = 12). The standard addition method was used to determine the terbium in a synthetic rare-earth sample with satisfactory results. This method represents a direct, rapid, selective and sensitive analytical procedure with a widely linear range. Received November 29, 2001 Revision February 9, 2002     

Study of the Fluorescence Enhancement of Eu-Y-DEM-Dibenzoylmethane-Ammonia System

Abstract

The fluorescence enhancement and solvent effect of Eu-dibenzoylmethane-ammonia system was studied. The fluorescence intensity of the system can be greatly increased by y³⁺. The maximum fluorescence intensity was obtained at pH 8.9 – 9.5. The fluorescence intensity is a linear function of Eu²⁺ in the range of 1.0x10^?9 M -4.0x10^?8 M. The detection limit is 1.1x10^?10 M. The optimized procedure is applied to the determination of europium in lanthanide oxide samples.     

Europium speciation by time-resolved laser-induced fluorescence

Time-resolved laser-induced fluorescence has been used to investigate Eu complexes formed with a few main ligands encountered in natural waters: hydroxide, carbonate and humic substances. By varying pH and concentrations of ligands at fixed europium concentration and ionic strength, it was possible, together with free europium Eu³⁺, to identify spectrally and temporally carbonate complexes, namely Eu(CO₃)⁺, Eu(CO₃)₂⁻ and Eu(CO₃)₃³⁻ and humate complexes EuHA. For hydroxide complexes, no differences were found in terms of fluorescence spectra and lifetimes. A spectral interpretation is described by using deconvolution for all systems.     

Photo-and Electro-luminescence of the New Ternary Europium(III) Complex

In recent years, the interest in developing luminescent lanthanide complexes has been greatly stimulated by their potential use in electroluminescent displays1. Europium complexes appear most attractive in view of the high photoluminescent efficiency and the high monochromatic red light that are widely exploited in full-color displays2. It has been approved that the second ligand plays an important role in europium complexes, the synergistic complexation of the second ligand can not only lead …     

Fluorimetric Determination Of Selenium In Marine Geological Materials

Abstract

Acid decomposition with nitric, perchloric and/or hydrofluoric acids is used to extract selenium from marine geological materials. Selenium is isolated from potentially interfering elements by coprecipitation with lanthanum hydroxide, then a piazselenol is formed between 2, 3-diaminonaphthalene at pH 1.0 and extracted into cyclohexane. The selenium is determined fluorimetrically in the extract. The detection limit is 3.2 ng (four times the standard deviation of blank) and the coefficient of variation is 5% at the 10 ng level. The sample decomposition procedure is evaluated and typical results obtained for selected samples are given.     

镉-铽-铕-强力霉素-曲通X-100协同荧光增敏体系研究

研究了在表面活性剂TritonX 10 0存在下 ,稀土离子和部分主、副族元素的离子对Eu 强力霉素(DC)体系的协同荧光增敏效应 ,建立了Cd Tb Eu DC TritonX 10 0协同荧光增敏体系 ,并提出了一种高灵敏度测定稀土元素铕的荧光光度新方法。方法的检出限为 5 .0× 10 -11mol/L ;线性范围为 4 .0× 10 -10 ～ 4 .0× 10 -8mol/L。     

Studies on the Fluorescence Enhancement Effect of Eu-La-DBM-DPG-Acetone System

The study of fluorescence enhancement has attracted attention recently. For example, the sensitivity and selectivity of Eu-thenoyltrifluoroacetone (TTA)phenan-throline and Eu-diphacinone-ammonia systems in Triton X-100 micelles are increased by the addition of La~(3+) Gd~(3+), Tb~(3+), Lu~(3+) or Y~(3+). The mechanism of this fluorescence(?)     

吡嗪－2，3－二羧酸体系导数荧光法同时测定铕、铽

研究了以吡嗪－２，３－二羧酸（ＰＹＤＡ）为荧光试剂应用导数荧光技术同时测定Ｅｕ~（３＋）、Ｔｂ~（３＋）的最佳条件、共存离子的影响和Ｅｕ~（３＋）、Ｔｂ~（３＋）－ＰＹＤＡ配合物的配合比、稳定常数及发光机理。Ｅｕ~（３＋）、Ｔｂ~（３＋）的检测限分别达４．０和２．０ｎｇ／ｍＬ，利用本方法成功地测定了稀土试样中铕、铽的含量。     

Fluorimetric Determination of Gentamicin in Dosage Forms and Biological Fluids Through Derivatization with 4-Chloro-7-Nitrobenzo-2-Oxa-1,3-Diazole (NBD-Cl)

 A highly sensitive fluorimetric method has been developed for the determination of gentamicin. The method is based on its coupling with 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) in 50% (v/v) methanolic phosphate buffer (pH 7.2) to give an intensely fluorescent product having excitation and emission wavelengths at 465 nm and 530 nm, respectively. The experimental parameters were carefully studied and incorporated into the procedure. The fluorescence-concentration plot is rectilinear over the range 0.56–2.8 μg/mL (r = 0.999) with minimum detectability (S/N = 2) of 0.11 μg/mL (1.6 × 10⁻⁷ mol/L). The method was applied successfully to the determination of the drug in pharmaceutical dosage forms and the percentage recoveries were satisfactorily accurate and precise. The method was further applied to spiked human plasma samples, the percentage recovery was 97.9 ± 3.4. The interference encountered from endogenous amino acids could be eliminated through selective complexation with freshly prepared copper (II) hydroxide. A proposal of the reaction pathway is presented. The method can measure the intact drug, and can be used in presence of possible interference. Received April 18, 2001; accepted April 10, 2002     

铕-结晶紫缔合物分光光度法测定痕量铕

在碱性(pH10.10)条件下,铕(Ⅲ)与结晶紫形成缔合物,表观摩尔系数为=1.8×105L/(mol.cm),铕的浓度在0.15~1.2mg/l范围内遵守比耳定律,组成摩尔比为Eu∶CV=1∶2.     

Luminescence Determination of Europium in High-Purity Yttrium and Gadolinium Oxides

ABSTRACT

A luminescence spectrometric method was developed for the determination of ultratrace amounts of europium (down to 1×10^?10 M) in high-purity yttrium and gadolinium oxides. This is based on the enhanced luminescence of europium thenoyltrifluoroacetone (TTA)-Tri-n-octyl phosphine oxide (TOPO)-Triton X-100 in the presence of terbium. The luminescence intensity is linear with europium concentration in the range 1×10^?9 to 1×10^?6 M under the recommended conditions. The optimized procedure is successfully utilized for the determination of ultratrace amounts of europium in yttrium and gadolinum oxides.