非水介质毛细管电泳电导检测罗红霉素及其制剂

采用甲醇为分离介质，三(羟甲基)氨基甲烷-硼酸(Tris—H3BO3)为支持电解质，采用负高压，使用电导检测，对罗红霉素及其制剂进行了毛细管电泳分离检测，对电泳介质的种类、浓度、表观pH、以及操作电压和进样时问对分离的影响进行了研讨，在选定的条件下，罗红霉素的线性范围为19．0—142．0mg/L，检出限为0．8mg/L(S／N≥3)，峰面积的相对标准偏差RSD(n=6)为4．3％。3种供试品中罗红霉素的平均加标回收率分别为97．7％、94．8％、93．6％。     

非水介质毛细管电泳

评述了与水相体系相比,非水体系电泳分离体系对分析对象的扩展、分离度和分离效率的改善、选择性的提高以及质谱检测联用技术等各方面带来的好处,同时还总结了非水介质各种参数对毛细管壁双层ζ电位、电渗流以及分离效率、分离度的影响。     

非水介质毛细管电泳电导法检测盐酸胺碘酮

采用非水介质毛细管电泳电导检测法对盐酸胺碘酮进行检测。探讨了缓冲溶液的种类、pH和浓度、分离电压、进样时间、进样高度等因素对检测效果的影响,建立了测定盐酸胺碘酮的新方法。用乙醇作为非水介质,在30mmol L三羟甲基氨基甲烷 15mmol L柠檬酸(pH6.90)运行缓冲溶液中,盐酸胺碘酮在5～200mg L范围内的线性回归方程为y=74.94x-7.83,r=0 999。检出限(S N=3)为0.5mg L,样品回收率为98.9%。适用于含盐酸胺碘酮的药剂的分析。     

高速毛细管电泳安培法检测甲巯咪唑及其制剂

采用高速毛细管电泳安培法对甲巯咪唑(TMZ)及其制剂的测定进行了研究; 通过优化检测电位、毛细管长度和内径、分离电压、缓冲溶液等实验参数, TMZ在60 s内可以得到较好的分离, 线性范围在2.00×10-3～2.80×10-5 mol/L, 检出限为3.50×10-6 mol/L; 峰面积和迁移时间的相对标准偏差分别为2.7%、 1.2% (n＝8); 该法可用于制剂中TMZ的检测.     

非水介质毛细管电泳电导检测虎杖中的白藜芦醇

分离检测;非水介质毛细管电泳电导检测虎杖中的白藜芦醇     

毛细管电泳的微机化安培法检测

毛细管电泳的微机化安培法检测胡深，李培标，程介克（武汉大学化学系，武汉，４３００７２）关键词毛细管电泳；微电极；安培法检测；微型计算机安培法电化学检测器用于毛细管电泳有独特的优点，如线性范围宽，选择性好，可以进行生物微环境电活性物质的分离检测［１，２...     

高效毛细管电泳安培检测的进展

本文对高效毛细管电泳电化学检测方法中的安培检测进行了评述，安培检测具有灵敏度高，选择的特点，安培检测根据毛细管内径的大小有离柱安培检测和柱端安培检测，近年来脉冲安培分析法、化学修饰电极也已被引入毛细管电泳电化学检测。     

毛细管电泳安培检测技术进展

对毛细管电泳离柱和柱端安培检测方式、不同形式电极在安培检测中的应用、安培检测在芯片毛细管电泳中的应用、安培检测池等内容进行了总结和讨论 ,并预测了安培检测技术未来发展方向     

毛细管电泳安培法检测酚类化合物

使用自行设计组装的毛细管电泳柱端安培检测系统 ,对四个酚类化合物进行了分离检测。研究了工作电极、缓冲液及其 p H值、检测电压和分离电压对分离检测的影响。在优化条件下 ,4个酚在 5× 1 0 -6～ 5× 1 0 -4 mol/L范围内峰高与浓度成良好的线性关系 ,检测下限为 8.5× 1 0 -7mol/L     

Determination of Roxithromycin Tablets by Capillary Electrophoresis Employing Non—aqueous Media with Square—wave Amperometric Detection

A new method of determination for roxithromycin tablets by non-aqueous capillary electrophoresis (NACE) with square-wave amperometric detection was carried out. Several parameters affecting the NACE-AD determination were studied. The data was modified by spline wavelet least square (SWLS). The method is simple, rapid and highly reliable for routine analysis.     

毛细管电泳安培检测法测定密蒙花中的黄酮类化合物

采用毛细管电泳-安培检测法(CE-AD)对蒙花苷、刺槐素、木犀草素和芹黄素四种黄酮类化合物进行分离分析.在电极电位为+0.95 V(vs.Ag/AgCl),电泳运行液为pH=9.00的60 mmol/L Na2 B4O7-120 mmol/L NaH2 PO4缓冲溶液,分离电压为18kV时,四种黄酮类化合物得到完全分离...     

毛细管电泳安培法分析单个神经细胞

毛细管电泳安培法分析单个神经细胞胡深，庞代文，王宗礼，程介克（武汉大学化学系，武汉，４３００７２）李之望，樊友珍，胡宏镇（同济医科大学实验医学研究中心，武汉，４３００３０）关键词毛细管电泳，安培法检测，单个细胞分析在生命科学研究中，单个水平的神经细胞...     

Capillary Zone Electrophoresis of Eleven Priority Phenols with Amperometric Detection

A scheme for separation and detection of eleven priority phenols using capillary zone electrophoresis (CZE) coupled with amperometric detection is described. With a capillary of I.D. 50 μm and length 62.5 cm at 9 kV and an electrophoretic buffer of 20 mM CHES (pH 10.1), complete separation of the eleven compounds was achieved in less than 17 min. Amperometric detection was carried out using a carbon fiber microelectrode of diameter 9 μm inserted into the end of the detection capillary. Linearity over two orders of magnitude was generally obtained for the eleven priority phenols. With an electrode potential+1.10 V (vs. Ag/AgCl reference), the concentration limits of detection were in the sub-ppm (10^?6 M) level. This method was successfully applied to analysis of priority phenols in industrial waste water.     

Determination of Psoralen and Isopsoralen in Traditional Chinese Medicines by Capillary Zone Electrophoresis with Amperometric Detection

A simple, reliable and reproducible method was developed for determination of psoralen and isopsoralen in several traditional Chinese medicines, such as Shouwu Pian, Gubenkechuan Pian, was described in this paper. It was based on capillary zone electrophoresis with amperometric detection (CZE-AD). A carbon-disc electrode was used as working electrode. The optimal conditions of separation and detection were pH7.6 phosphate buffer (20 mM), 20 kV for the separation voltage and 1.00V (vs. Ag/AgCl) for the detection potential. The linear ranges were 6.90 × 10^-5~1.30 × 10^-7 mol L^-1for psoralen and 5.0 × 10^-5~1.80 × 10^-7 mol·L^-1 for isopsoralen with the correlation coefficient of 0.9984 and 0.9994, respectively. The detection limits for psoralen and isopsoralen were 4.2 × 10^-8 mol·L^-1 and 6.0 × 10^-8 mol·L^-1 based on the signal to noise ratio of 3. The method built in this paper was directly applied to the determination of several traditional Chinese medicines with some simple pretreatment. The assay result was satisfactory.     

Simultaneous Determination of Catecholamines and Related Metabolites by Capillary Electrophoresis with Amperometric Detection

An electrophoretic method was developed for the determination of several important catecholamine markers, namely norepinephrine, epinephrine, dopamine, metanephrine, vanillylmandelic acid and homovanillic acid in urine samples. Under the optimum conditions, the six marker compounds could be well separated with the major coexisting interference compound uric acid within 24 min at a separation voltage of 16 kV in a borate running buffer (80 mmol/L, pH=9.48). Highly linear response can be obtained over three orders of magnitude for the above markers with the low limits of detection ranging from 1.0 ng/mL to 5.0 ng/mL(S/N=3). The proposed capillary electrophoresis with amperometric detection(CE-AD) method has been used to simultaneously determine the six catecholamine markers in urine samples of healthy volunteers and patients suffering from different diseases avoiding redundant measurements and high assay cost; and the electrochemical profiles can suggest more diagnostic information of multiple diseases, which provides a promising and convenient entry into primary diagnoses of several clinical diseases.     

Separation and Determination of Clenbuterol,Cimaterol and Salbutamol by Capillary Electrophoresis with Amperometric Detection

Capillary electrophoresis with amperometric detection was applied to determine some β₂‐agonists, such as clenbuterol, cimaterol and salbutamol in this paper. The working electrode used was a 0.3 mm diameter carbon disk electrode. In this work, the pH 6.0–6.4 borax‐potassium dihydrogen phosphate was used as running buffer (150 mmol/L), 10 kV as the separation voltage and 1.05 V (vs. Ag/AgCl, 3 mol/L KCl) as the detection potential. Under the optimum conditions, the analytes were baseline separated within 16 min. Linear range for cimaterol, clenbuterol and salbutamol was 1.0–2000, 2.0–2000 and 1.0–2000 ng/mL, respectively. The detection limits (define as 3σ/k) were 0.5, 1.0 and 0.4 ng/mL for cimaterol, clenbuterol and salbutamol, respectively. The developed method has been applied to determine these three analytes in feed and urine by standard addition. The mean recoveries for these three analytes ranged from 89.0% to 102.0%.     

非水体系毛细管电泳-紫外检测法测定对乙酰氨基苯酚的研究

本文报道了用非水相体系高效毛细管电泳-紫外检测法测定对乙酰氨基苯酚的新方法;考察了运行电压、非水相介质和电解质等因素的影响。在25℃下,以50mmol／L NaOH的乙醇溶液为电泳缓冲介质,30mmol／L乙酸钠的乙醇溶液为样品溶剂;选择重力进样30S,运行电压-25kV,及检测波长250nm。测定对乙酰氨基苯酚的线性范围为5．2～52μg／mL;检出限为2．88μg／mL;RSD为2．34％。本法应用于维C银翘片中对乙酰氨基苯酚的测定,结果满意。     

毛细管电泳-方波安培法分离检测滴鼻液中的麻黄碱

在熔融石英毛细管 (75mm× 5 0cm )中 ,以 5mmol/LTris(三羟甲基氨基甲烷 ) +5mmol/LH3 BO3(pH =6 .5 )为电泳介质 ,采用毛细管电泳 方波安培检测法 ,实现了滴鼻液中盐酸麻黄碱的分离检测。探讨了缓冲溶液的种类、浓度、pH值、检测电位等因素对分离检测效果的影响。线性范围为 0 .8～ 2 0 2mg/L ,检出限为 0 .3mg/L ,回收率为 92 %～ 10 4 %。