N-磷酸化修饰蛋白质的富集和鉴定方法

蛋白质磷酸化修饰在细胞的信号转导、代谢、发育等生命过程中发挥着重要作用。除了研究较为透彻的发生在丝氨酸、苏氨酸和酪氨酸侧链羟基的O-磷酸化修饰之外,近年来,发生在组氨酸、精氨酸和赖氨酸侧链氨基的N-磷酸化修饰受到了越来越广泛的关注。然而,由于N-磷酸化修饰具有独特的P-N键结构,导致其化学稳定性差。尤其是在O-磷酸化肽段富集常用的酸性条件下,N-磷酸化极易丢失。因此,目前对N-磷酸化蛋白质的研究仍处于初始阶段。该文针对蛋白质N-磷酸化修饰的特点、富集和鉴定方法进行了综述,并对其发展前景进行了展望。     

Synthesis of polyfluoralkylated 1,3,2-dioxaphospholane and 1,3,2-dioxaphosphorinane oxides

Polyfluoroalkyl dichlorophosphates easily react with 1,2- or 1,3-alkanediols in a system pyridine–diethyl ether and afford 2-polyfluoroalkoxy-1,3,2-dioxaphospholane oxides or 2-polyfluoroalkoxy-1,3,2-dioxaphosphorinane oxides. In similar conditions the reaction of methyl dichlorophosphate with 1,2- and 1,3-alkanediols proceeds less effectively.     

Iminophosphorane-mediated transformation of tertiary alcohols into t-alkylamines and their N-phosphorylated derivatives

Novel azidation of tertiary alcohols by means of trimetylsilyl azide in the presence of boron trifluoride etherate has been worked out. The Staudinger reaction of crude azides with triethyl phosphite affords the corresponding iminophosphoranes which can be directly transformed into diethyl N-(t-alkyl) phosphoroamidates or t-alkylamine tosylates.     

Synthesis and characterization of tetraphenylphosphonium salts of N-phosphorylated thioureas and bis-thioureas

Russian Chemical Bulletin - Tetraphenylphosphonium salts of N-phosphorylated thioureas and bio-thioureas were synthesized by the reactions of their sodium salts with PPh4Br in 96% aqueous EtOH. The...     

2-Alkylperhydro-1,3,2-dioxazepines

Perhydro-1,3,2-dioxazepines were obtained for the first time by the acid-catalyzed cyclization of acyclic N,N-dialkoxyamines.Translated from Izvestiya Akademii Nauk SSSR, Seriya Khimicheskaya, No. 10, pp. 2382–2384, October, 1989.     

2-Polyfluoroalkoxy-1,3,2-dioxaphosphorinanes

2-Polyfluoroalkoxy-1,3,2-dioxaphosphorinanes and related phosphocyclic compounds were prepared by alcoholysis of 2-amino-1,3,2-dioxaphosphorinanes with polyfluorinated alcohols. Principal chemical properties of the products were studied. For the first time copper complexes of fluorine-containing normal phosphites were prepared. A difference in the ligation of copper with fluorinated and nonfluorinated phosphites was shown.     

4,5-Benzo-1,3,2-thiazaphospholanes

Conclusions 2-Substituted 3-methyl-4,5-benzo-l,3,2-thiazaphospholanes were obtained in high yields by the reaction of N-methyl-o-aminothiophenol with phosphorous acid di- and triamides.Translated from Izvestiya Akademii Nauk SSSR, Seriya Khimicheskaya, No. 12, pp. 2837–2839, December, 1976.     

1，3，2—二氮磷杂环戊烷类化合物的合成

为了寻找新的除草剂,我们研究了N,N′-二取代苯磺酰乙二胺与不同类型磷化合物的反应(图1)。实验表明取代乙二胺不能与亚磷酸酯反应,而与亚磷酰胺反应,发现产物及其硫化物具有一定的除草活性。     

The chemistry of 1,3,2-diazaborolines (2,3-dihydro-1H-1,3,2-diazaboroles)

1,3,2-Diazaborolines (2,3-dihydro-1H-1,3,2-diazaboroles) are at the interface between inorganic, organometallic and organic chemistry. The planar rings with 6π-electrons may be regarded as heteroarenes, as evidenced by NMR and photoelectron spectra and confirmed by quantum mechanical calculations. The capability of 1,3,2-diazaborolines to act as η⁵-ligands adds chemical proof to this idea. High-yield syntheses of 1,3,2-diazaborolines with functional groups at boron have recently become available, providing a rich area of chemistry ranging from substitution processes via borylstannations to their conversion into oxazaborolidines.