Isolation and characterization of phytokinin-binding proteins from the cotton plant

A highly purified site of a cytokinin-binding protein with a K_D value for³H-BAP of 4.3 nM has been isolated from cotton-plant seedlings by the methods of biospecific and hydrophobic chromatography. According to the results of electrophoretic analysis under denaturing conditions its molecular mass is 43 kDa. A. S. Sadykov Institute of Bioorganic Chemistry, Uzbekestan Academy of Sciences, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 702–706, November–December, 1992.     

Water-soluble ethylene-binding proteins of the cotton plant: Isolation and functional activity

A protein possessing a high affinity for ethylene has been isolated from cottonplant shoots by hydrophobic chromatography on Polikhrom-1 and gel chromatography on TSK gel HW-50F. A number of proofs of the receptor nature of the protein isolated are given.S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax 627071. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 92–96, January–February, 1994.     

Isolation and characterization of protease inhibitors from cotton seeds

Protease inhibitors of protein nature have been isolated from dormant cotton seeds. The participation of protease inhibitors in the mechanism of protecting the plant from wilt damage is discussed.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent, fax (3712) 89 14 75. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 445–448, May–June, 1995. Original article submitted June 27, 1994.     

Isolation of immunosimilar proteins from cotton seeds by immunoaffinity chromatograph

A sorbent highly specific forVerticillium proteins has been obtained from BrCN-Sepharose and rabbit immunoglobulins. By affinity chromatography using this sorbent a protein immunologically similar to the proteins of the mycelium of the fungusV. dahliae has been isolated from cotton seeds of the Tashkent-1 variety. The molecular mass of the protein has been determined, and its proteolytic activity has been established. Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 746–749, September–October, 1993.     

Isolation of immunosimilar proteins from cotton seeds by immunoaffinity chromatograph

A sorbent highly specific forVerticillium proteins has been obtained from BrCN-Sepharose and rabbit immunoglobulins. By affinity chromatography using this sorbent a protein immunologically similar to the proteins of the mycelium of the fungusV. dahliae has been isolated from cotton seeds of the Tashkent-1 variety. The molecular mass of the protein has been determined, and its proteolytic activity has been established.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Republic of Uzbekistan, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 746–749, September–October, 1993.     

Isolation of hemigossypol quinone from a cotton plant infected with wilt

Summary From the stems of the cotton plant infected with the fungusVerticillium dahliae Kleb. has been isolated a quinone derivative of hemigossypol — hemigossypolone — for which the structure 8-formyl-6,7-dihydroxy-5-isopropyl-3-methyl-1,4-naphthoquinone is proposed, and its fungitoxicity has been determined.Division of Bioorganic Chemistry of the Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 341–344, May–June, 1977.     

Isolation of the phospholipids of the cotton plant and their composition

Summary The yields of the free and bound lipids of the seed kernels of cotton plants of varieties S-4727 and S-6029 have been determined by their successive treatment with selective solvent, and the total and fractional proportions of lipid phosphorus have been found.The phospholipids have been solated and their group composition has been determined; it has been established that the main components are phosphatidylcholines, phosphatidylinositols, and phosphatidylethanolamines, while polyglycerophosphatides, lysophosphatidylcholines, and unidentified polar phospholipid are present in small amounts.The fatty-acid compositions of the free and bound lipids and phospholipids have been studied.Institute of the Chemistry of Plant Substances, Academy of the Uzbek SSR. Translated from Khimiya Prirodnykh Soedinenii, No. 4, pp. 449–453, July–August, 1975.     

Conformational changes in the reserve proteins of the cotton plant

A quantitative study of the secondary structures of the 11S and 7S globulins from cotton seeds has been made by the circular dichroism (CD) method. It has been established that the 11S and 7S globulins contain, respectively: 16 and 14% of -helices; 15% each of -structures; 18 and 20% of -bends; and 51% each of irregular sections.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Institute of Molecular Biology, Academy of Sciences of the USSR, Moscow. Translated from Khimiya Prirodnykh Soedinenii, No. 3, pp. 355–357, May–June, 1984.     

Study of the membrane proteins of the cotton plant using monoclonal antibodies

The total membranes from cottonplant shoots have been used as the antigenic material for obtaining monoclonal antibodies (mcABs). Eleven stable hybrids producing mcABs to components of the membrane proteins have been selected. Antibodies to various membrane fractions and to fractions of lectin-like proteins have been revealed. Structural characteristics of the carbohydrate components of cottonplant glycoproteins have been determined by cross-immunochemical analysis.A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, Fax 627071. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 83–87, January–February, 1994.     

Isolation of ribosomes from cotton seeds

Summary 1. It has been established that the method of precipitation with ethanol in the presence of an increased concentration of Mg²⁺ can be used for the rapid isolation of the ribosome fraction from plant material.2. The optimum conditions for the complete precipitation of the ribosome fraction from a homogenate of cotton seeds have been selected.3. By treating an extract of the seeds with 0.5 M KCl it was possible to reduce the amount of tRNA in a preparation of the total rRNA.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 6, pp. 852–856, November–December, 1977.     

Isolation of the total tRNA from cotton seeds

Summary 1. A comparative study has been made of various methods of isolating the tRNA from cotton seeds. It has been found that the best results are given by extraction of cotton seeds with a mixture of 80% phenol and 0.14 M NaCl.2. The conditions have been worked out for the preparative isolation of the total tRNA.Institute of the Chemistry of Plant Substances, Academy of Sciences of the Uzbek SSR, Tashkent. Translated from Khimiya Prirodnykh Soedinenii, No. 5, pp. 621–624, September–October, 1978.