共查询到20条相似文献,搜索用时 15 毫秒
1.
Water ligand observed via gradient spectroscopy (WaterLOGSY), saturation transfer difference and NOE pumping NMR techniques were used to identify ligand binding with a receptor. Although these experiments were originally designed to observe ligands in complexes, their application is limited by the affinity of ligands towards target molecules. Here the improved WaterLOGSY pulse sequence was developed by incorporating the double pulsed field gradient spin-echo and gradient-tailored excitation WATERGATE sequences. The efficiency of these ligand-observed NMR screening techniques was investigated using the ribonuclease T1-inhibitor system. 相似文献
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C. Santambrogio F. Favretto M. D'Onofrio M. Assfalg R. Grandori H. Molinari 《Journal of mass spectrometry : JMS》2013,48(8):i-i
Human liver fatty acid binding protein (hL‐FABP) is the most abundant cytosolic protein in the liver. This protein plays important roles associated to partitioning of fatty acids (FAs) to specific metabolic pathways, nuclear signaling and protection against oxidative damage. The protein displays promiscuous binding properties and can bind two internal ligands, unlike FABPs from other tissues. Different topologies for the ligand located in the more accessible site have been reported, with either a ‘head‐in’ or ‘head‐out’ orientation of the carboxylate end. Electrospray‐ionization mass spectrometry and nuclear magnetic resonance titrations are employed here in order to investigate in further detail the binding properties of this system, the equilibria established in solution and the pH dependence of the complexes. The results are consistent with two binding sites with different affinity and a unique head‐out topology for the second molecule of either ligand. Competition experiments indicate a higher affinity for oleic acid relative to palmitic acid at each binding site. Copyright © 2013 John Wiley & Sons, Ltd. 相似文献
3.
A novel approach for characterizing protein ligand complexes: molecular basis for specificity of small-molecule Bcl-2 inhibitors 总被引:5,自引:0,他引:5
Lugovskoy AA Degterev AI Fahmy AF Zhou P Gross JD Yuan J Wagner G 《Journal of the American Chemical Society》2002,124(7):1234-1240
The increasing diversity of small molecule libraries has been an important source for the development of new drugs and, more recently, for unraveling the mechanisms of cellular events-a process termed chemical genetics.(1) Unfortunately, the majority of currently available compounds are mechanism-based enzyme inhibitors, whereas most of cellular activity regulation proceeds on the level of protein-protein interactions. Hence, the development of small molecule inhibitors of protein-protein interactions is important. When screening compound libraries, low-micromolar inhibitors of protein interactions can be routinely found. The enhancement of affinities and rationalization of the binding mechanism require structural information about the protein-ligand complexes. Crystallization of low-affinity complexes is difficult, and their NMR analysis suffers from exchange broadening, which limits the number of obtainable intermolecular constraints. Here we present a novel method of ligand validation and optimization, which is based on the combination of structural and computational approaches. We successfully used this method to analyze the basis for structure-activity relationships of previously selected (2) small molecule inhibitors of the antiapoptotic protein Bcl-xL and identified new members of this inhibitor family. 相似文献
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Virus-ligand interactions: identification and characterization of ligand binding by NMR spectroscopy
Benie AJ Moser R Bäuml E Blaas D Peters T 《Journal of the American Chemical Society》2003,125(1):14-15
We demonstrate the detection and characterization of ligand binding to viruses via NMR. To illustrate the methodology, the interaction of an antiviral compound with human rhinovirus serotype 2 (HRV2) was investigated. Specific interaction of a capsid-binding inhibitor and native HRV2 was monitored utilizing saturation transfer difference (STD) NMR. STD NMR experiments at atomic resolution allowed those regions of the ligand that are involved in the interaction with the virus to be determined. The approach allows for (i) the fast and robust assessment of binding, (ii) the determination of the ligand binding epitope at atomic resolution without the necessity to crystallize virus-ligand complexes, and (iii) the reuse of the virus in subsequent assays. This methodology enables one to easily identify binding of drugs, peptides, and receptor or antibody fragments to the viral capsid. 相似文献
5.
Kouvatsos N Meldrum JK Searle MS Thomas NR 《Chemical communications (Cambridge, England)》2006,(44):4623-4625
We have engineered a variant of the beta-clam shell protein ILBP which lacks the alpha-helical motif that caps the central binding cavity; the mutant protein is sufficiently destabilised that it is unfolded under physiological conditions, however, it unexpectedly binds its natural bile acid substrates with high affinity forming a native-like beta-sheet rich structure and demonstrating strong thermodynamic coupling between ligand binding and protein folding. 相似文献
6.
In the past few years, NMR has been extensively utilized as a screening tool for drug discovery using various types of compound libraries. The designs of NMR specific chemical libraries that utilize a fragment-based approach based on drug-like characteristics have been previously reported. In this article, a new type of compound library will be described that focuses on aiding in the functional annotation of novel proteins that have been identified from various ongoing genomics efforts. The NMR functional chemical library is comprised of small molecules with known biological activity such as: co-factors, inhibitors, metabolites and substrates. This functional library was developed through an extensive manual effort of mining several databases based on known ligand interactions with protein systems. In order to increase the efficiency of screening the NMR functional library, the compounds are screened as mixtures of 3-4 compounds that avoids the need to deconvolute positive hits by maintaining a unique NMR resonance and function for each compound in the mixture. The functional library has been used in the identification of general biological function of hypothetical proteins identified from the Protein Structure Initiative. 相似文献
7.
Rademacher C Krishna NR Palcic M Parra F Peters T 《Journal of the American Chemical Society》2008,130(11):3669-3675
The analysis of virus-receptor interactions at atomic resolution is of fundamental importance to understand infection processes, and to establish novel anti-viral therapies. As an example, rabbit hemorrhagic disease virus (RHDV), a member of the Caliciviridae family and considered as an "emerging" virus, attaches to histo-blood group antigens (HBGA) on the surface of adult rabbit epithelial cells of the upper respiratory and digestive tracts. It appears that this attachment is a key step in the process of infection with RHDV. Here, we report NMR experiments that reveal the atomic details of the recognition of HBGAs and fragments thereof by RHDV virus-like particles (VLP). The experiments yield binding epitopes of several HBGAs and show that L-fucose is a minimal structural requirement for specific molecular recognition by the VLPs. As the methodology is general, these studies may pave the way for the development of novel anti-viral entry inhibitors. 相似文献
8.
Qing Dai Chun-Yan Xu Yusuke Sato Keitaro Yoshimoto Seiichi Nishizawa Norio Teramae 《Analytical sciences》2006,22(2):201-203
The recognition ability of pteridine derivatives for nucleobases opposite an abasic (AP) site in an oligodeoxynucleotide (ODN) duplex is enhanced by using a propylene residue (Spacer-C3) as an AP site. The recognition ability is further enhanced both by attaching methyl groups to a fluorescent ligand and by measuring the fluorescence response at 5 degrees C; 6.2 x 10(6) M(-1) of the binding constant is attained between 2-amino-6,7-dimethyl-4-hydroxypteridine and guanine opposite the AP site in water. 相似文献
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[reaction: see text] Here we describe a miniature protein (1) that presents the cAMP-dependent protein kinase (PKA) recognition epitope found within the heat-stable Protein Kinase Inhibitor protein (PKI) and a miniature protein conjugate (1-K252a) in which 1 is joined covalently to the high-affinity but nonselective kinase inhibitor K252a. Miniature protein 1 recognizes PKA with an affinity that rivals that of PKI and, in the context of 1-K252a, leads to a dramatic increase in kinase specificity. 相似文献
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Tiago Vicente José P.B. Mota Cristina Peixoto Paula M. Alves Manuel J.T. Carrondo 《Journal of chromatography. A》2010,1217(13):2032-2041
Surface plasmon resonance (SPR) spectroscopy is used as a scaled-down, analytical, pseudo-chromatography tool for analyzing protein binding and elution over an ion-exchange surface under cyclic sorption conditions. A micrometric-scale adsorption surface was produced by immobilizing a typical ion exchange ligand – diethylaminoethyl (DEAE) – onto commercially available planar gold sensor chip surfaces pre-derivatized with a self-assembled monolayer of 11-mercaptoundecanoic acid with known density. An explicit mathematical formulation is provided for the deconvolution and interpretation of the SPR sensorgrams. An adsorption rate model is proposed to describe the SPR sensorgrams for bovine serum albumin, used here as model protein, when the DEAE surface is subjected to a cyclic series of binding and elution steps. Overall, we demonstrate that the adsorption rate model is capable of quantitatively describing BSA binding and elution for protein titers from dilute conditions up to overloaded conditions and a broad range of salt concentrations. 相似文献
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Wiseman RL Johnson SM Kelker MS Foss T Wilson IA Kelly JW 《Journal of the American Chemical Society》2005,127(15):5540-5551
Protein native state stabilization imposed by small molecule binding is an attractive strategy to prevent the misfolding and misassembly processes associated with amyloid diseases. Transthyretin (TTR) amyloidogenesis requires rate-limiting tetramer dissociation before misassembly of a partially denatured monomer ensues. Selective stabilization of the native TTR tetramer over the dissociative transition state by small molecule binding to both thyroxine binding sites raises the kinetic barrier of tetramer dissociation, preventing amyloidogenesis. Assessing the amyloidogenicity of a TTR tetramer having only one amyloidogenesis inhibitor (I) bound is challenging because the two small molecule binding constants are generally not distinct enough to allow for the exclusive formation of TTR.I in solution to the exclusion of TTR.I(2) and unliganded TTR. Herein, we report a method to tether one fibril formation inhibitor to TTR by disulfide bond formation. Occupancy of only one of the two thyroxine binding sites is sufficient to inhibit tetramer dissociation in 6.0 M urea and amyloidogenesis under acidic conditions by imposing kinetic stabilization on the entire tetramer. The sufficiency of single occupancy for stabilizing the native state of TTR provides the incentive to search for compounds displaying striking negative binding cooperativity (e.g., K(d1) in nanomolar range and K(d2) in the micromolar to millimolar range), enabling lower doses of inhibitor to be employed in the clinic, mitigating potential side effects. 相似文献
16.
Mathias EV Liu X Franco O Khan I Ba Y Kornfield JA 《Langmuir : the ACS journal of surfaces and colloids》2008,24(3):692-700
Rf-IPDU-PEGs belong to a class of fluoroalkyl-ended poly(ethylene glycol) polymers (Rf-PEGs), where the IPDU (isophorone diurethane) functions as a linker to connect each end of the PEG chain to a fluoroalkyl group. The Rf-IPDU-PEGs form hydrogels in water with favorable sol-gel coexistence properties. Thus, they are promising for use as drug delivery agents. In this study, we introduce an electron-spin induced 19F relaxation NMR technique to probe the location and drug-loading capacity for an electron-spin labeled hydrophobic drug, CT (chlorambucil-tempol adduct), enclosed in the Rf-IPDU-PEG micelle. With the assistance of molecular dynamics simulations, a clear idea regarding the structures of the Rf-IPDU-PEG micelle and its CT-loaded micelle was revealed. The significance of this research lies in the finding that the hydrophobic drug molecules were loaded within the intermediate IPDU shells of the Rf-IPDU-PEG micelles. The molecular structures of IPDU and that of CT are favorably comparable. Consequently, it appears that this study opens a window to modify the linker between the Rf group and the PEG chain for achieving customized structure-based drug-loading capabilities for these hydrogels, while the advantage of the strong affinity among the Rf groups to hold individual micelles together and to interconnect the micellar network is still retained in hopes of maintaining the sol-gel coexistence of the Rf-PEGs. 相似文献
17.
Buchmueller KL Staples AM Howard CM Horick SM Uthe PB Le NM Cox KK Nguyen B Pacheco KA Wilson WD Lee M 《Journal of the American Chemical Society》2005,127(2):742-750
Pyrrole (Py) and imidazole (Im) polyamides can be designed to target specific DNA sequences. The effect that the pyrrole and imidazole arrangement, plus DNA sequence, have on sequence specificity and binding affinity has been investigated using DNA melting (DeltaT(M)), circular dichroism (CD), and surface plasmon resonance (SPR) studies. SPR results obtained from a complete set of triheterocyclic polyamides show a dramatic difference in the affinity of f-ImPyIm for its cognate DNA (K(eq) = 1.9 x 10(8) M(-1)) and f-PyPyIm for its cognate DNA (K(eq) = 5.9 x 10(5) M(-1)), which could not have been anticipated prior to characterization of these compounds. Moreover, f-ImPyIm has a 10-fold greater affinity for CGCG than distamycin A has for its cognate, AATT. To understand this difference, the triamide dimers are divided into two structural groupings: central and terminal pairings. The four possible central pairings show decreasing selectivity and affinity for their respective cognate sequences: -ImPy > -PyPy- > -PyIm- approximately -ImIm-. These results extend the language of current design motifs for polyamide sequence recognition to include the use of "words" for recognizing two adjacent base pairs, rather than "letters" for binding to single base pairs. Thus, polyamides designed to target Watson-Crick base pairs should utilize the strength of -ImPy- and -PyPy- central pairings. The f/Im and f/Py terminal groups yielded no advantage for their respective C/G or T/A base pairs. The exception is with the -ImPy- central pairing, for which f/Im has a 10-fold greater affinity for C/G than f/Py has for T/A. 相似文献
18.
The concept used to realize the modulation of molecular recognition in a molecularly imprinted polymer is presented. Creating a thermal phase transition within the binding framework, the imprinted material was prepared using Boc-phenylalanine as the template and pNIPAM as the sensitive unit. The results indicate that such a transition causes a clear modulation in the recognition behavior of the prepared polymer which depends on the operation temperature. At a relatively low temperature, the prepared polymer acts like a traditionally imprinted one, showing a highly specific recognition for the imprint species. However, the prepared polymer does not present any notable resolution at 40 degrees C. This recognition behavior is comparable to a process that can be switched on and off, thus making modulated recognition feasible. 相似文献
19.
Cage-type cyclophanes, which are constructed with two rigid 2,11,20-triaza[3.3.3]paracyclophane skeletons and three chiral bridging components, were prepared. Temperature-dependent 1H NMR measurements in (CD3)2SO indicate that the molecular framework of the cage-type cyclophane having a cylindrical internal cavity is more rigid than those of the corresponding non-cage hosts. The guest-binding behavior of the cage-type hosts toward various guests was examined by electronic absorption spectroscopy and electrospray ionization (ESI) mass spectrometry. The present hosts were found to bind anionic guests, such as 1-hydroxy-2,4-dinitronaphthalene-7-sulfonate, 2-hydroxy-1-(phenylazo)naphthalene-6,8-disulfonate, 2,7-bis[(4-methyl-2-sulfophenyl)azo]-1,8-dihydroxynaphthalene-3,6-disulfonate, 8-anilinonaphthalene-1-sulfonate, 6-p-toluidinonaphthalene-2-sulfonate, naphthalene-1-sulfonate, and 3,5-bis(methoxycarbonyl)benzene-1-sulfonate, to form host-guest complexes. The computer-aided molecular modeling study reveals that the three pyridinium moieties bound to the chiral
- and
-valine residues in the bridging segments undergo chiral twist in the same directions. However, the twisted direction in the host bearing
-valine residues is opposite to that evaluated for the host bearing
-valine residues so that the former and latter cage-type cyclophanes furnish M and P-helical cavities, respectively, as reflected in their circular dichroism (CD) spectra. The chirality-based molecular recognition of the cage-type hosts toward enantiomeric guests such as bilirubin-IX and pamoic acid in aqueous media was investigated by CD spectroscopy. 相似文献