毛细管电泳非接触电导检测技术的新发展

毛细管电泳非接触电导检测(CE-CCD)是近年来发展迅速的一种检测技术。本文介绍了非接触式电导检测(CCD)的发展概况,着重阐述了CCD的原理、组成及应用情况。     

毛细管电泳及芯片毛细管电泳的电容耦合非接触电导检测

综述了毛细管电泳(CE)及芯片毛细管电泳(MCE)的电容耦合非接触电导检测(Capacitively Coupled Contactless Conductivity detection,C4D)的研究状况;并分别对其装置、检测的影响因素及其应用进行了评述。引用文献81篇。     

毛细管电泳涂层研究进展

近年来,随着毛细管电泳与质谱、激光诱导荧光检测等联用技术的飞速发展,毛细管电泳技术在生命科学、环境保护、食品检验等领域得到广泛应用.对毛细管内壁进行涂层改性是提高毛细管电泳的分离效果和重现性,抑制分析物与毛细管内壁间吸附作用的最有效、最常用的方法.该文根据涂层材料的种类和制备机理,分别综述了近年来非共价键合和共价键合毛...     

毛细管电泳电化学检测

综述了毛细管电泳的电化学检测,包括电位法、电导法和安培法检测的研究进展,重点讨论了电化学检测与毛细管电泳的耦联,并对电化学检测的原理及其应用进行了较详细的叙述,引用文献72篇。     

高效毛细管电泳电导检测器的研制

研制了一种毛细管电泳电导检测器。采用激光烧蚀毛细管涂层、HF腐蚀和阴离子交换膜封堵制作在柱导电接口连接电泳毛细管和电导池,高压电场被有效隔离,以铂丝为工作电极实现柱后电导检测,在内径为50μm毛细管上分离检测了几种氨酸和金属离子,结果表明该系统性能优良。     

体液中卡托普利的乙醚提取毛细管电泳法测定

体液中卡托普利的乙醚提取毛细管电泳法测定;毛细管电泳;高频电导检测;非接触式电导检测;卡托普利;体液     

毛细管电泳-荧光/非接触电导组合型检测器的研制

报道了一种毛细管电泳-荧光／非接触电导组合型检测器。该检测器共用非接触电导检测池,实现了双检测器响应同步。优化了非接触电导检测系统中激发电压信号及其频率;荧光检测是用发光二极管作为激发光源,用光纤收集并传输荧光信号至光电倍增管。用无机金属离子和异硫氰酸荧光素评价该体系,结果表明,该检测器达到了任一单类型检测器性能指标。     

微芯片毛细管电泳电化学检测方法及应用

阐述了微芯片毛细管电泳电化学检测(包括安培法、电导法、电化学发光法和联用电化学法)的研究进展；对各种电化学检测的原理和应用进行了较详细的叙述；着重讨论了不同材料检测电极在安培检测中的应用；接触式电导和非接触式电导的应用情况；展望了微芯片毛细管电泳电化学检测的前景。引用文献87篇。     

一种简易型毛细管电泳电导检测装置及其应用

报道了一种简便、实用的毛细管电泳电导检测装置 ,该装置使用毛细管微铂丝为敏感电极 ,大面积铂电极为参比电极 ,并以一根不锈钢针管为连接导管 ,成功地实现了毛细管电泳的柱端式电导检测 ,并对K 、Na 、Li 3种碱金属离子进行了分离检测。实践表明 ,该装置具有制作简单且成功率高、更换电极方便的特点。在所选的实验条件下 ,3种金属离子在1×10 -5～1×10 -3mol/L浓度范围内电泳峰与浓度呈良好线性关系 ,检出限为5×10-6 mol/L。应用该法对城市自来水样品进行了测定 ,其重现性和检测结果令人满意     

毛细管电泳安培及电导检测最新进展

对毛细管电泳安培检测和电导检测的研究近况进行了综述,分别介绍了电导法和安培法与毛细管电泳的联接及应用,并对其发展方向作了展望。     

Carbon nanotube and diamond as electrochemical detectors in microchip and conventional capillary electrophoresis

As two important polymorphs of carbon, carbon nanotube (CNT) and diamond have been widely employed as electrode materials for electrochemical sensing. This review focuses on recent advances and the key strategies in the fabrication and application of electrochemical detectors in microchip and conventional capillary electrophoresis (CE) using CNT and boron-doped diamond. The subjects covered include CNT-based electrochemical detectors in microchip CE, CNT-based electrochemical detectors in conventional CE, boron-doped diamond electrochemical detectors in microchip CE, and boron-doped diamond electrochemical detectors in conventional CE. The attractive properties of CNT and boron-doped diamond make them very promising materials for the electrochemical detection in microchip and conventional CE systems and other microfluidic analysis systems.     

Electrochemical Detection for Capillary Electrophoresis Microchips: A Review

Electrochemistry detection offers considerable promise for capillary‐electrophoresis (CE) microchips, with features that include remarkable sensitivity, portability, independence of optical path length or sample turbidity, low cost and power requirements, and high compatibility with modern micromachining technologies. This article highlights key strategies in controlled‐potential electrochemical detectors for CE microchip systems, along with recent advances and directions. Subjects covered include the design of the electrochemical detection system, its requirements and operational principles, common electrode materials, isolation from the separation voltage, derivatization reactions, typical applications, and future prospects. It is expected that electrochemical detection will become a powerful tool for CE microchip systems and will lead to the creation of truly portable (and possibly disposable) devices.     

芯片毛细管电泳电化学检测

评述了芯片毛细管电泳各种电化学检测尤其是安培检测中工作模式、工作电极、分离电流的消除、应用等方面的进展,并进行了展望。     

毛细管电泳-电容耦合非接触电导检测方法

介绍了电容耦合非接触电导检测(C4D)的检测原理及其最新的研究进展,引用文献50篇。C4D是近几年发展起来的一种用于毛细管电泳和微流控芯片电泳的新检测技术。C4D检测器的原理清楚,结构简单,易于微型化、集成化,不污染检测电极,因而很有应用价值。     

Integrated circuit microchip system with multiplex capillary electrophoresis module for DNA analysis

In this paper, we describe the use of an integrated circuit (IC) microchip system as a detector in multiplex capillary electrophoresis (CE). This combination of multiplex capillary gel electrophoresis and the IC microchip technology represents a novel approach to DNA analysis on the microchip platform. Separation of DNA ladders using a multiplex CE microsystem of four capillaries was monitored simultaneously using the IC microchip system. The IC microchip-CE system has advantages such as low cost, rapid analysis, compactness, and multiplex capability, and has great potential as an alternative system to conventional capillary array gel electrophoresis systems based on charge-coupled device (CCD) detection.     

Pyrolyzed Photoresist Carbon Electrodes for Microchip Electrophoresis with Dual‐Electrode Amperometric Detection

The fabrication and evaluation of pyrolyzed photoresist films (PPF) for microchip capillary electrophoresis (CE) with dual‐electrode electrochemical (EC) detection is described. The sensitivity, linearity, and reproducibility were evaluated using catecholamines and related compounds, including dopamine (DA), 5‐hydroxyindole‐3‐acetic acid (5‐HIAA), ascorbic acid (AA), and catechol. Initial studies with DA show the response of the PPF electrodes to be linear between 25 and 500 μM (r²=0.999) with a limit of detection (LOD) of 5 μM (S/N=3) and sensitivity of 5.8 pA/μM. Selectivity was further enhanced by employing dual‐electrode detection in the series configuration for detection of species exhibiting chemically reversible redox reactions.     

微量金属元素的毛细管电泳分析方法及应用

综述了毛细管电泳分析微量金属元素的基本原理、分离模式(CZE、MKEC、非水电泳、芯片分离等)、检测方法(紫外、荧光、化学发光、安培、电导、质谱联用技术)等的进展和该技术在环境、生物医学领域的研究与应用。引用文献94篇。     

Carbon paste-based electrochemical detectors for microchip capillary electrophoresis/electrochemistry

The first reported use of a carbon paste electrochemical detector for microchip capillary electrophoresis (CE) is described. Poly(dimethylsiloxane) (PDMS)-based microchip CE devices were constructed by reversibly sealing a PDMS layer containing separation and injection channels to a separate PDMS layer that contained carbon paste working electrodes. End-channel amperometric detection with a single electrode was used to detect amino acids derivatized with naphthalene dicarboxaldehyde. Two electrodes were placed in series for dual electrode detection. This approach was demonstrated for the detection of copper(II) peptide complexes. A major advantage of carbon paste is that catalysts can be easily incorporated into the electrode. Carbon paste that was chemically modified with cobalt phthalocyanine was used for the detection of thiols following a CE separation. These devices illustrate the potential for an easily constructed microchip CE system with a carbon-based detector that exhibits adjustable selectivity.     

芯片毛细管电泳中组分的迁移行为及其特征

在自组装的芯片毛细管电泳-激光诱导荧光检测装置上,以单个染料和一组荧光素异硫氰酸酯(FITC)标记的氨基酸为对象,研究了芯片毛细管电泳与传统毛细管电泳之间的差别,考察了玻璃芯片上微通道内的伏安特性以及抑制电压、进样方式和检测点的位置等对芯片毛细管电泳分离分析的影响,特别注意到了其有别于传统毛细管电泳的各种行为特征.