Capillary Zone Electrophoretic Separation of Basic Proteins with Coated Columns Prepared by Sol-Gel Technology

l.1ntroductionItiswellknownthatthehighresolvingpowerofcaPillnyzoneelectrophoresistendstodecreaseserious1yin.theseparaionofproteinsduetotheadsorptionofproteinsontheinnerwallsoffusedsilicacaPillaries,whichleadtoconsiderablepeakbroadeningandpoorreproducibility[l].AtpresentseveralapproacheskavebeenemployedtoelhoinatethewalladsorptionofproteinsamongwhichcoatingthecaPillarysurfacewithaneutralweaklyhydrophilicpolyInercanalwaysgiveexcellent,esultS[2'3].Thispaperdescribesaone-stepmethodforthepreparai…     

Separation and Determination of Bases by Capillary Zone Electrophoresis

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Separation of Peptides by Pressurized Capillary Electrochromatography

A novel gradient pressurized capillary electrochromatography (pCEC) instrument was developed to separate peptides.Two gradient elution modes,hydrophobic and hydrophilic interaction mode in pCEC,were performed on this instrument.Baseline separation of six peptides was obtained on two gradient modes with C18 column and strong cationic exchange column respectively.The effects of mixer volume and total frow rate of pumps on resolution were also discussed.     

Electrochemical Determination of Cortisol by Capillary Electrophoretic Enzyme Immunoassay

In recent years, capillary electrophoretic immunoassay (CEIA) has become a primary analytical tool in clinical diagnostics. The primary detection methods in CEIA are UV absorbance detection and laser induced fluorescence detection (LIF)1. The major disadvantages of the UV detector are the lack of sensitivity and not available for many antigens which are small molecules without strong UV absorbance. LIF is a more general approach to improve sensitivity. However, it is difficult to u…     

Enantiomeric Separation and Determination of R,S-Salsolinol by Capillary Electrophoresis

A new method for the quantitative determination of the enantiomers of salsolinol, an endogenous neurotoxin, was reported. Enantiomeric separation of salsolinol was obtained by capillary zone electrophoresis using hydroxypropyl-β-cyclodextrin （HP-β-CD） as chiral selector. Coupled with UV detection, this separation was applied to the determination of R,S-salsolinol in dried banana chips, and both R- and S-salsolinol were found at 40 ktg/g level.     

Chiral Separation of Esmolol and Terazosin by Cyclodextrinmodified Capillary Zone Electrophoresis

IntroductionTheincreasingnecessity0fpharmacologicalresearchforchiraldrugshasrenderedchiralseparationanactiveareaincaPillaryelectroph0resis(CE)today.Variousmodes0fCE,includingcaPillaryzoneelectroph0resis(CZE),micellarelectrokineticchromatography(MEKC),caPillarygelelectrophoresis(CGE)andcapillaryisotachophoresis(CITP),havebeenappliedforthispurp0se',amongwhichCZEisthemostpopularoneforitshighefficiency,lowexpenseandeaseofoperation.Forsuccessfulchiralseparati0ninCZE,theselectortypeisofcr…     

On-line Separation and Detection of Peptides by Capillary Electrophoresis / Electrospray FT-ICR-MS

Recently, capillary electrophoresis/electrospray ionization mass spectrometry (CE/ESI- MS) has been rapidly developed as a powerful analytical tool for charged species ranged from small molecules such as carboxylic acids1, phenolic compounds2, metal species3, tetramines4, herbicides5, drugs and drug metabolites6 to peptides and proteins7, 8. The ESI mode has proven to be sensitive, versatile and relatively easy to use in combination with CE. CE confers rapid analysis and efficient separ…     

Separation of Aniline Derivatives by Micellar Electrokinetic Capillary Chromatography

A micellar electrokinetic capillary chromatography (MECC) was developed for the determination of aniline and 6 substituted anilines. The seven components were separated within 25min in the buffer solution of 40mmol/L sodium borate and 100mmol/L SDS. It was found that the separation was dependent on operating voltage, pH value, borate and SDS concentrations.The analytical performance was examined in terms of linear response and reproducibility.Wastewater was determined by the established method.     

On-line Separation and Detection of Peptides by Capillary Electrophoresis/Electrospary FT-ICR-MS

The coupling of capillary electrophoresis to electrospray Fourier transfrom ion cyclotron resonance mass spectrometry (FT-ICR-MS) was presented for the on-line separation and detection of mixture of peptides.With the ultra-high resolution and unmatchable mass measurement accuracy FT-ICR-MS provides,the separation and identification of angiotension Ⅲ,octreotide and elastin chemotactic were successfully achieved using this fabricated device,which showed its great application potential in detection and structural identification for multicomponent sample.     

DNA Separation by Capillary Electrophoresis with Ultraviolet Detection using Mixed Synthetic Polymers

The mixtures of two polymers, poly (N,N-dimethylacrylamide) (PDMA) and polyvinylpyrrolidone (PVP) were synthesized and used as the separation medium for double-stranded and single-stranded DNA fragments by capillary electrophoresis with UV detector. On optimal conditions, 2%w/v PDMA 2%w/v PVP can be used to separate the doublet 123/124bp in pBR322/Hae Ⅲ Markers.     

Molecularly Imprinted Polymer Monolithic Column Separation of Isomers and Analogues of Vanillin by Capillary Electrochromatography

A vanillin imprinted capillary monolithic column was synthesized by in situ polymerization reaction using ethylene-glycol dimethacrylate as cross-linking monomer and methacrylic acid as functional monomer. Under the optimum conditions of capillary electrochromatography, this molecularly imprinted polymer （MIP）-based column showed high selectivity and could recognize not only template molecule vanillin but also positional isomer o-vanillin from their structural analogues.     

Determination of Thyroxine with Capillary Electrophoretic Enzyme Immunoassay

Capillary electrophoretic immunoassay (CEIA) is a new analytical technique1,2. In CEIA, the detection methods were UV absorbance detection1 and laser induced fluorescence detection (LIF)2. However, The UV detection is lack of sensitivity. The LIF is difficult to use due to its high cost, and furthermore, most biological fluids are strongly luminescent when excited by the laser in the blue or green region of the spectrum. In our present work, a capillary electrophoretic enzyme immuno…     

Chiral Separation of N-Benzoyl Phenylalanine Methyl Ester by Nonaqueous Capillary Electrophoresis

Theseparationofopticalisomersisarapidlygrowingareaincapillaryelectrophoresis(CE),andmostchiralseparationbyCEhavebeenperformedinaqueousbackgroundelectrolyte.Organicsolventssuchasmethanolandacetonitrilehavebeenappliedasbuffermodifiersatconcentrationtypicallynothigherthan40%inordertoincreasehydrophobicanalytesolubilityandtoimproveselectivity,resolution,ortoaltertheelectroosmoticflow(EOF)andelectrophoreticmobilityofanalyte.Buttherehavebeenonlyafewreportsonthechiralseparationusingpurenonaqueousme…     

Carboxymethyl-β-Cyclodextrin for Chiral Separation of Basic Drugs by Capillary Elcctrophoresis

Theseparationofchiralcompoundsisofkeyimportanceindifferentareasofresearch,e,g.,pharmaceutical,biological.,environmentaletc.'Capillaryelectrophoresis(CE)isapowerfultechniqueforchiralreparation.ThegeneralmeritsofCEsuchasimpressivepeakefficiency,rapidityofanalysieismallvolumesandlowcostsarethemostimportantfactorsjcontributingtothesucclessfuldevelopmentofthistechnique"=.Currentlycyclodextrins(CDs)arethemostcommonlyusedchiralresolvingagentsinCE.Enantiomericseparationisbasedonthedifferenceinincl…     

Optimized Separation of Pharmacologically Active Xanthones from Securidaca inappendiculata by Capillary Electrophoresis

Securidaca inappendiculata Hassk. is a traditional Chinese herbal medicine. Pharma- cological investigations have shown that the xanthones, as main components accumulated in S. inappendiculata have many bioactivities, such as monoamine oxidase inhibition, antitumor activity, cytotoxicity, antibacterial activity, antifungal activity, anti-inflammatory properties, antioxidant activity and tuberculoatatic activity, etc1. The contents of xanthones are very important and key factors for quality …     

Further Study on Separation of DNA Fragments by Capillary Electrophoresis by Quasi-interpenetrating Network of Polyacryamide and Polyvinylpyrrolidone with UV Detection

Quasi-interpenetrating network of polyacrylamide （PAA） and polyvinylpyrrolidone （PVP） had been successfully used for single-base resolution of double-stranded DNA （0.76 for 123 bp/124 bp） and single-stranded DNA fragments （0.97 for 123 b/124 b） with UV detection. This quasi-IPN （interpenetrating network） sieving matrix showed low viscosity （23.5 mPa·s at 25 ℃） and decreased with increasing temperature. This polymer also exhibited dynamically coating capacity and could be used in the uncoated capillary. The effects of temperature and electric field strength on the DNA separation of quasi-IPN matrix were also investigated and found that the temperature and electric field strength could markedly affected the mobility behavior of DNA fragments. This polymer matrix has also applied to separate the bigger DNA fragments by capillary electrophoresis with UV detection. Under the denaturing conditions, this matrix separated the samples with last fragment of 1353 base in 40 rain, in which the doublet of 309/310 base was partial separated and the resolution was 0.88.     

Optimized Separation of Pharmacologically Active Xanthones from Secuidaca inappendiculata by Capillary Electrophoresis

A capillary electrophoresis (CE) method has been firstly used for the separation of the therapeutically important xanthones from Securidaca inappendiculata. The separation of the nine xanthones was systematically optimized with respect to pH, concentration of running buffers,addition of sulfated β-CD,applied voltage and column temperature.Baseline separation was achieved for the nine xanthones in less than 15 minutes using a background electrolyte consisting of 200 mmol/L borate (pH9.5) and 10 mmol/L sulfated β-CD.     

Comparison of Carboxylic and Hydroxycarboxylic Acids as Complexing Agents for Transition Metal Separation with Nonaqueous Capillary Electrophoresis

Many papers have been published on the analysis of metal cations by capillary electrophoresis (CE), for example, lanthanide, transition metal, alkali, and alkaline earth metal ions. Separations of metal ions are based on the differences in their electrophoretic mobilities. Since most     

Study on the Interaction between Strychnine and Bovine Serum Albumin by Capillary Electrophoretic Frontal Analysis

Most drugs are usually bound to proteins such as serum albumin,α1-acid glycoprotein,lipoprotein,and other blood constituents.The plasma concentration of an unbound drug shows better correlation to the pharmaceutical activity1.It is therefore necessary to know the extent of drug-protein binding in order to adjust the optimal therapeutic dose of the drug.Strychnos nux-vomica L.(Loganiaceae)is an evergreen tree native to southeast Asia.Its dried seeds are used for treatment of nervous diseases…