一种转基因猪血中重组人血清白蛋白分离纯化新方法

基因工程技术已经成为研究和生产重组人血清白蛋白(rHSA)替代人血清白蛋白(HSA)的重点技术,而白蛋白的纯化则是该技术的关键。本文主要介绍了从转基因猪血中纯化rHSA的一种新方法,即热乙醇沉淀与多级色谱分离相结合的rHSA纯化方法。热乙醇沉淀法可从猪血浆中获得rHSA粗提取液,此时rHSA的纯度可达69.5%,回收率达51.3%。进一步采用多级色谱分离法,即阴离子交换色谱和反相色谱法进一步纯化,得到rHSA的最终纯度约为100.0%,总回收率为41.1%。该方法为从转基因猪血浆中大规模纯化用于临床和生化研究的高纯度rHSA提供可能,同时也为rHSA替代HSA奠定了基础。     

A Novel Method of Preparing Unsymmetrical Thioether from Disulfides

The disulfides reacted with zinc in DMF, followed by alkyl halides, giving unsymmetrical thioether in excellent yields. This reaction takes place under mild and neutral conditions.     

快速测定酶法动力学拆分中转化率和ee值的新方法

在动力学拆分反应中 ,转化率和对映体过量值( ee值 )是经常需要测量的两个重要参数 .传统的测定转化率的方法包括色谱法、分光光度法和滴定法等 .而测定 ee值主要应用手性色谱柱、手性毛细管电泳、核磁以及旋光法等 .在动力学拆分过程中 ,需要对反应进程进行监测 ,而应用传统的方法进行监测需要同时测定两个量 (转化率和 ee值 ) ,工作量较大 ,且使用手性色谱柱进行监测成本也较高 ,不适合大量实验数据的常规分析 .为此 ,需要发展一种快速、灵活、经济的测量方法 ,用以实验前期筛选条件时的常规分析 .基于此思路 ,我们借助于自己编制的计算机…     

A Novel Electrocatalysis Method for Organic Pollutants Degradation

As typical contaminants, phenolic pollutants are wide spread and are toxic to aquatic life. Thus the demand for new technology to remove or detoxify such organics should be widely recognized. Recently, electrochemical method has attracted a great deal of attention for treating such kinds of wastewater, mainly because of its amenability to automation, high efficiency and environmental compatibility1. Various electrochemical processes have been developed and anodic oxidation might be the most w…     

一种顶空气相色谱测定乳液聚合过程单体转化率的新方法

介绍了一种通过顶空气相色谱技术测定乳液聚合过程中单体转化率的新方法.通过对聚合乳液样品进行称量方式的采样和大比例的稀释,将已稀释的待测溶液移入顶空瓶中,在设定温度下达到气液平衡后进入气相色谱中测定,其消除了采样的波动性和顶空气相色谱测定的误差,并导出了由顶空气相色谱对样品中单体的测定信号直接计算聚合过程转化率的方程式.该方法具有实验操作简单、快速、检测准确等优点,非常适合乳液聚合反应动力学和机理的研究.     

A Novel Method for Fabrication of a Glass-Electrode-Based Lipase Sensor

Lipase is one of the hydrolases. It catalyzes the hydrolysis of triglycerides, liberating fat acids. Based on its catalysis and the response of a glass electrode to the acids, a glass-electrode-based lipase sensor can be fabricated1, which has great potential in clinical medicine for the detection of blood fat in human serum samples.The immobilization of lipase on the bulb of a glass electrode is the key to the development of the sensor. In addition to its response time, which has a lot to do …     

Novel Synthetic Method for Triacetylbenzene

Triketones are an important class of compounds in view of the distinct structure feature and wide utility in macromolecular material, organic synthesis and coordination chemistry1. The synthetic method for preparing symmetrical triketones 1, 3, 5- triacetylbenzene reported by Robert et al. is via condensation of acetone with ethyl formate followed by the trimerization of the intermediate acetylacetaldehyde2. We reported the reaction of benzimidazolium salts with Grignard reagents, providing a …     

A Novel Method for Modification of SMPU by Micro-phase Separation Promoters

Shape memory polyurethane(SMPU)is a typical temperature-sensitive functional polymer having an excellent shape memory behavior1,which has been extensively studied since19882,10.B.K.Kim et al.3reported the effect of hard segment contents(HSC)and soft segment length(SSL)of SMPU on its modulus,hardness and recovery strain.They attributed these effects to its micro-phase separation of its soft segment and hard segment as well as its soft segment crystallization.F.K.Li et al.4found that high…     

A Novel Method to Synthesize Polyorganozircosilazane

A new kind of polyorganozircosilazane as Si/Zr/C/N-based ceramic precursor was synthesized from the condensation reaction of hexamethyleyclotrisilazane lithium salts(D3^NLi）and zirconium tetrachloride(ZrCl4).In the presence of N,N,N’,N’-tetramethyl ethylene diamine(TMEDA),polyorganozircosilazane was obtained in high yield.     

A Novel Method for the Synthesis of Carboxylic Esters Catalyzed by Expandable Graphite

Esterification reaction of carboxylic acid and alcohol has been carried out in excellent yield with expandable graphite as a catalyst.