Tocainide Determination by High-Performance Liquid Chromatography

Abstract

Tocainide has been assayed after serum deproteinization with acetonitrile by HPLC. The pH was critical in separating the drug from other interferences in the serum. The method is simple and fast.     

Historical and radiopharmaceutical relevance of [18F]FDG

Journal of Radioanalytical and Nuclear Chemistry - The 2-[18F]fluoro-2-deoxy-d-glucose ([18F]FDG) is the most well-known radiopharmaceutical positron emitter, in both clinical and preclinical...     

Determination of Carbonyl Compounds by High-Performance Liquid Chromatography

Carbonyl compounds in the form of 2,4-dinitrophenylhydrazones in alcoholic solutions were analyzed by high-performance liquid chromatography. This simple procedure is characterized by high sensitivity and reproducibility.     

Determination of Monobromobimane-Labeled Phytochelatins by High-Performance Liquid Chromatography

A new method for phytochelatins by high-performance liquid chromatography (HPLC) was developed based on a condensation reaction with monobromobimane to produce fluorescent derivatives. Glutathione, H-(γ-glutamic acid-cysteine)₂-glycine-OH, H-(γ-glutamic acid-cysteine)₃-glycine-OH, H-(γ-glutamic acid-cysteine)₄-glycine-OH, H-(γ-glutamic acid-cysteine)₅-glycine-OH, and H-(γ-glutamic acid-cysteine)₆-glycine-OH were well separated, with retention times between 14.68 and 22.0 min. The HPLC method had good linearity (r < 0.9991) between 0.1 mg L^?1 and 100 mg L^?1. The limits of quantification for the analytes (S/N = 3) were 0.08, 0.3, 0.05, 0.3, 0.5, and 0.8 mg L^?1, respectively. The recoveries were between 83.0% and 101.33% with relative standard deviations less than 2%. The reported method is simple, accurate, and suitable for the determination of phytochelatins.     

高效液相色谱法测定环己铜过氧化物

以甲醇或甲醇与水作流动相,ODS为固定相,测定环己酮的多种过氧化物。平均回收率100.2％～101.6％,测定的相对标准偏差为1.02％～2.51％。     

高效液相色谱法测定邻苯二酚纯度

采用反相高效液相色谱法测定邻苯二酚纯度 ,以C18为固定相 ,甲醇∶0 .2 %乙酸 =5 0∶5 0 (V/V)为流动相 ,检测波长为 2 77nm。回收率为 94.6 %～ 10 1.8% ,标准偏差为 0 .19～ 0 .2 4(n =8)。     

Simultaneous Determination of Pioglitazone and Glimepiride by High-Performance Liquid Chromatography

A rapid and accurate HPLC method has been developed for simultaneous determination of pioglitazone and glimepiride. Chromatographic separation of the two pharmaceuticals was performed on a Cosmosil C₁₈ column (150 mm × 4.6 mm, 5 m) with a 45:35:20 (v/v) mixture of 0.01 m triammonium citrate (pH adjusted to 6.95 with orthophosphoric acid), acetonitrile, and methanol as mobile phase, at a flow rate of 1.0 mL min^–1, and detection at 228 nm. Separation was complete in less than 10 min. The method was validated for linearity, accuracy, precision, limit of quantitation, and robustness [1, 2]. Linearity, accuracy, and precision were found to be acceptable over the ranges 2.50–30.00 g mL^–1 for pioglitazone and 0.10–10.00 g mL^–1 for glimepiride.     

Simultaneous Determination of Food-Related Amines by High-Performance Liquid Chromatography

Abstract

Ten amines found in marine foods, dimethylamine, trimethylamine, trimethylamine oxide, ammonia, urea, histamine, cadaverine, putrescine, spermine and spermidine were separated by HPLC using an ion-moderated partition column. Optimum resolution and sensitivity were obtained using 0.003N sodium hydroxide as the mobile phase and UV detection at 208 nm.     

Determination of Tetracycline and Related Compounds by High-Performance Liquid Chromatography

Abstract

An isocratic high-performance liquid chromatography method for the determination of tetracycline and its related compounds is described. The method uses a reverse phase (C₁₈) column, a modified acetonitrile/water mobile phase, and banzoic acid as the internal standard. Elution of all compounds of interest is complete within seven minutes. Results are presented for thirteen commercial capsule formulations and are compared with results by microbiological assay and thin-layer chromatographic methods.     

Quick and Simple Determination of Dihydroergotamine by High-Performance Liquid Chromatography

Abstract

A simple and rapid liquid chromatographic assay method using a fluorescence detector for quantitation of dihydroergotamine in plasma without extraction was developed. After precipitating the protein with acetonitrile, the supernatant liquid was directly injected for analysis. Chromatographic separation was achieved on C₁₈ reversed phase column and the mobile phase was the isocratic mixture of methanol, acetonitrile and glycine buffer (0.5:3.5:6.0). With this eluting solvent the drug and its internal standard were well separated from the interference of the plasma sample. The average recovery of dihydroergotamine from 6 replicate samples of different concentrations (5-30 ng/ml) were 92.2 ± 3.37%. The minimum amount of dihydroergotamine detectable by this method was 2 ng/ml of sample.     

高效液相色谱法测定肉制品中甘草抗氧化物的含量

建立了测定肉制品中甘草抗氧化物含量的方法。样品经流动相提取,采用C．8色谱柱分离,以甲醇-0．2mol／L乙酸铵-冰乙酸（体积比70：29：1）为流动相,流速为1．0mL／min,波长为250nm,以保留时间进行定性,峰面积进行定量。甘草酸在1．0-80．0μg／mL范围内线性关系良好,回归方程为y=8．55×10^-5x-0．599（r=0．9997）,检出限为1．0mg／kg,定量限为3．0mg／kg,加标回收率为95．7％-103．4％,测定结果的相对标准偏差为1．57％-3．53％（n=6）。该方法适用于检验机构日常检验中大批量肉制品中甘草抗氧化物含量的测定。     

Determination of Plasma Levels of Bupivacaine by High-Performance Liquid Chromatography

Abstract

We evaluate a method for determination of bupivacaine using HPLC, and the possibility of pharmacological interferences produced by seven commonly used drugs administered before, during and after surgery: diacepam, midazolam, epinephrine, naloxone, flumacenil, atropine and ephedrine. The method has an average recovery of 102.8 +/? 5.4%. The detection limit is 0.125 μg/mL. The within-day coefficient of variation is 5.88% and the between-day coefficient of variation is 15%. We haven't found drug interferences at generally encountered serum concentrations.     

甲壳素纯度的高效液相色谱法测定

由于生物材料中甲壳素或壳聚糖是大分子，不溶于水，以及与蛋白质等结合在一起而难以定量测定，工业生产中尚无甲壳素或壳聚糖纯度的标准测定方法；采用甲壳素水解生成氨基葡萄糖，再用氯甲酸芴甲酯(FMOC—C1)进行衍生，利用液相色谱技术对衍生产物进行分析；结果表明，HPLC法是生物材料中甲壳素的一种灵敏的、特有的定性定量分析方法，为生物材料及工业化生产中甲壳素纯度的测定提供了一个方便的手段。     

高效液相色谱法测定血清中茶碱浓度

采用高效液相色谱,分析柱：３μｍ,３．３ｃｍ＊４．６ｍｍ,Ｉ．Ｄ（Ｐｅｒｋｉｎ Ｅｌｍｅｒ,ＵＳＡ）;预柱：１０μｍ,１ｃｍ＊２．１ｍｍ,Ｉ．Ｄ（Ｐｅｒｋｉｎ ＥＬｍｅｒ,ＵＳＡ）;以乙酰氨基酚为内标对氯仿－异丙醇（９５：５,Ｖ／Ｖ）提取样品进行了分析,流动相：０．１ｍｏｌ／Ｌ醋酸缓冲液（ＰＨ＝４．５）－甲醇（７０：３０,Ｖ／Ｖ）;检测波长：２７０ｎｍ;流速０．５ｍＬ／ｍｉｎ,３ｍｉｎ即完成一次茶     

Determination of Pyridostigmine in Human Plasma by High-Performance Liquid Chromatography

Abstract

An easy to perform, specific, reproducible and sensitive high performance liquid chromatographic (HPLC) method to measure pyridostigmine concentration in human plasma was developed and validated. Sample clean-up consists of ion-pair extraction into dichloromethane in the presence of neostigmine as internal standard, followed by back extraction into an aqueous phase. Mean recovery of 110% (with a standard deviation of 10%) was determined for concentrations of 5 – 100 ng/ml. Chromatography on a 125·4 mm CN-propyl column using a mobile phase composed of 10% acetonitrile in 3.5×10^?4M NaH₂PO₄ and UV detection at 270 nm, yields clean chromatograms without any interferences from endogenous plasma components. Using 1 ml plasma samples the method has a limit of detection (LD) of 3 ng/ml, with %CV (precision) and bias (accuracy) ≥ 10% for concentrations in the range of 0–100 ng/ml. The method is being used in human pharmacokinetic studies of oral dosage forms of pyridostigmine.     

Determination of Cefpirome in Human Plasma by High-Performance Liquid Chromatography

Abstract

Cefpirome is an investigational third-generation cephalosporin, which appears promising for the treatment of various pediatric infections. A high performance liquid chromatographic method was developed to measure cefpirome in small volumes of plasma for conducting pharmacokinetics studies in infants and children. the assay involved precipitation of plasma proteins with acetonitrile, using cefaclor as an internal standard. Chromatographic separation was accomplished using a reverse-phase     

Determination of Whiteners in Cosmetics by Microdialysis and High-Performance Liquid Chromatography

A simple method was developed for the determination of hydrophilic whitening agents (ascorbyl glucoside, magnesium ascorbyl phosphate, arbutin, hydroquinone, and kojic acid) in bleaching cosmetics by high performance liquid chromatography with electrochemical detection. The samples were prepared using microdialysis and were separated within ten minutes using isocratic elution. Linear calibration was achieved for concentrations between 0.10 and 10.00 micromolar. The limits of detection were from 0.6 to 2.0 nanomolar; the recoveries were between 91.43 and 108.04 percent, and the relative standard deviation was less than 6.88 percent. This method was used to determine whitening compounds in several cosmetics with on-line microdialysis and the zero net flux method. The procedure was fast, simple, selective, and suitable for routine analysis.     

Determination of Acyclovir in Human Serum by High-Performance Liquid Chromatography

Abstract

A method was developed for determination of concentrations of acyclovir in serum. Serum proteins were precipitated with equal part of 5% perchloric acid. High-performance liquid chromatography was used for separation from endogenous compounds and detection was done with spectrophotometry. The assay is simple and precise and seems well suited for pharmacokinetic studies.     

Determination of Serum Phospholipid Metabolic Profiles by High-Performance Liquid Chromatography

Abstract

A method for the separation of serum phospholipid molecular species by reversed-phase high-performance liquid chromatography is described. Total serum phospholipid extracts were hydrolyzed with phospholipase C and benzoylated, and the resulting monoglyceride, diglyceride, and ceramide derivatives were separated by using gradient elution with detection at 230nm. Both human and rat serum phospholipid extracts were examined, and the use of this method for the evaluation of serum phospholipid metabolic alterations accompanying exposure to an environmental toxicant (polybrominated biphenyls) is described.     

高效液相色谱法测定有机肥中洛克沙胂

建立了高效液相色谱(HPLC)-串联紫外检测法检测有机肥中洛克沙胂的分析方法。选用20g·L-1K2HPO4作为提取剂,于60℃温度下超声提取有机肥中的洛克沙胂,采用MAX固相萃取柱净化。结果表明,采用Symmetry ShieldTMRP 18色谱柱,以含0.1%甲酸的0.05mol·L-1 KH2PO4∶甲醇=95∶5(V/V)溶液作流动相,等度洗脱,紫外266nm检测,洛克沙胂的保留时间短,且可完全避开杂质峰的干扰;其平均加标回收率介于81.14%~82.75%之间,相对标准偏差(RSD)7.0%,检出限为20μg·L-1。方法重现性好,精密度高,操作简便,适用于有机肥中洛克沙胂检测。