共查询到18条相似文献,搜索用时 156 毫秒
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TNT分子印迹聚合物微球的合成与性能研究 总被引:1,自引:0,他引:1
以三硝基甲苯(TNT)为模板分子,EDMA为交联剂,采用沉淀聚合法制备了TNT分子印迹微球.讨论了溶剂用量、模板分子用量、功能单体种类等对分子印迹微球的形貌及吸附性能的影响;利用紫外吸收光谱和BET表征了印迹聚合物微球的结合位点相互作用与印迹孔穴结构;通过平衡吸附和选择性吸附实验,研究了印迹聚合物微球的吸附性能和选择性识别性能.结果表明,以丙烯酰胺为功能单体制备的分子印迹聚合物为规则的球形,内部含有分子印迹孔穴,微球的粒径为1~2μm.印迹聚合物微球可在30 min内达到吸附平衡,在1 mmol/L的TNT乙醇溶液中,印迹聚合物微球的平衡吸附量为32.5 mmol/kg,对TNT分离系数为25.19,具有较好的特异性吸附能力,并可选择性识别TNT分子. 相似文献
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以表面修饰乙烯基团的SiO2微球为基体,白藜芦醇为模板分子,丙烯酰胺(AA)为功能单体,乙二醇二甲基丙烯酸酯(EGDMA)为交联剂,采用表面印迹技术制备核-壳型白藜芦醇印迹微球。采用红外光谱(IR)、扫描电子显微镜(SEM)对该分子印迹微球进行表征,结果表明,SiO2表面成功接枝一层厚度为200nm的印迹聚合物,该印迹微球颗粒分散均匀。采用高效液相色谱技术对印迹微球的吸附性进行研究表明,此印迹微球具有良好的识别性能,利用Scatchard模型分析得出印迹微球的最大吸附量分别为Qmax1=9.087mg/g和Qmax2=13.80mg/g。此印迹微球成功用于分离虎杖提取液中白藜芦醇。 相似文献
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以纤维素和纳米Fe3O4为原料制得磁性纤维素微球, 在纤维素微球表面选择合适的模板分子, 以甲基丙烯酸、 丙烯酰胺和N,N'-亚甲基双丙烯酰胺为功能单体, 采用水溶液聚合法制得表面分子印迹磁性纤维素微球. 采用傅里叶变换红外光谱(FTIR)、 X射线衍射(XRD)和振动样品磁强计(VSM)等表征了分子印迹聚合物微球的结构. 以罗丹明B(RhB)为模板分子, 通过吸附动力学与吸附热力学实验研究了表面分子印迹磁性纤维素微球对RhB的吸附性能, 结果表明, 制备的表面分子印迹磁性纤维素微球对罗丹明B具有特异性识别作用, 饱和吸附量达到0.542 mg/mg, 吸附平衡时间为10 h左右. 表面分子印迹磁性纤维素微球大大降低了对吸附环境的依赖, 并可重复利用. 相似文献
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壬基酚表面印迹聚合物微球的合成及分子识别特性 总被引:1,自引:0,他引:1
采用表面分子印迹技术,在二氧化硅微粒表面通过乙烯基三甲氧基硅烷接枝,以壬基酚(NP)为模板、α-甲基丙烯酸为功能单体制备了壬基酚印迹聚合物。扫描电镜及比表面分析仪测试结果表明制备的印迹聚合物呈均匀分散的微球,具有较大的比表面积。采用红外光谱表征印迹聚合物微球制备过程中的化学结构变化情况,并用平衡吸附法研究了聚合物对NP的结合性能与分子识别特性。研究结果表明,聚合物对壬基酚具有良好的结合亲和性,最大结合量可达184.6 mg/g。印迹聚合物对NP的吸附量高于其结构类似物对特辛基酚和双酚A的吸附量,表现出较高的选择性识别能力。 相似文献
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核-壳型厚朴酚印迹聚合物的制备及性能研究 总被引:1,自引:1,他引:0
以表面修饰功能基团的SiO2微球为基体,以厚朴酚为模板分子,丙烯酰胺为功能单体,丙烯酸乙二醇二甲酯为交联剂,在SiO2微球表面制备对厚朴酚具有较好选择识别能力的核-壳型印迹聚合物.采用红外光谱及扫描电镜等技术表征聚合物的结构及形态.结果表明,该印迹聚合物表面成功制备了壳层厚度约为200nm的均匀印迹层.通过静态吸附、Scatchard分析法以及竞争吸附实验研究了该聚合物的吸附性能和选择性,结果表明,它对厚朴酚形成均一结合位点,离解常数为0.19mg/mL. 相似文献
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表面接枝分子印迹聚合物微球的合成及评价 总被引:5,自引:0,他引:5
将聚苯乙烯-二乙烯苯微球表面功能基化, 引入引发转移终止剂(initiator-transfer-terminator agent, iniferter), 以活性自由基聚合方式研究了球形树脂表面合成印迹聚合物的方法. 在紫外光引发下, 以左旋麻黄碱为印迹分子, 甲基丙烯酸为功能单体, 在接枝Iniferter后的微球表面进行分子印迹聚合物接枝实验, 并使用不同的反应条件, 探讨了表面接枝印迹层微球制备条件对于识别能力的影响. 平衡吸附的结果表明, 表面接枝聚苯乙烯-二乙烯苯微球对于印迹分子具有亲和能力及选择性, 其识别能力来自于印迹得到的识别位点. 相似文献
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Qingqing Gai Qiuye Liu Wenyou Li Xiwen He Langxing Chen Yukui Zhang 《Frontiers of Chemistry in China》2008,3(4):370-377
Molecularly imprinted polymers (MIPs), based on photografting surface-modified polystyrene beads as matrices, were prepared
with acrylamide as the functional monomer, bovine hemoglobin as the template molecule and N, N′-methylene bisacrylamide as the crosslinker in a phosphate buffer. The results of IR, scanning electron microscope (SEM)
and elemental analyses demonstrated the formation of a grafting polymer layer on the polystyrene-bead surface. Subsequent
removal of the template left behind cavities on the surface of the polymer matrix with a shape and an arrangement of functional
groups having complementary binding sites with the original template molecule. The adsorption studies showed that the imprinted
polymers have a good adsorption capacity and specific recognition for bovine hemoglobin as the template molecule. Our results
demonstrated that the polymer prepared via the photografting surface-modified method exhibited better selectivity for the
template. Attempts to employ the new method in molecular imprinting techniques may introduce new applications for MIPs and
facilitate probable protein separation and purification.
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Translated from Chemical Journal of Chinese Universities, 2008, 29(1): 64–70 相似文献
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One‐step preparation of magnetic imprinted nanoparticles adopting dopamine‐cupric ion as a co‐monomer for the specific recognition of bovine hemoglobin 下载免费PDF全文
Ruixia Gao Lili Zhang Yi Hao Xihui Cui Dechun Liu Min Zhang Yuhai Tang 《Journal of separation science》2015,38(20):3568-3574
A novel magnetic core–shell polydopamine–cupric ion complex imprinted polymer was prepared in one‐step through surface imprinting technology, which could specifically recognize bovine hemoglobin from the real blood samples. The polymerization conditions and adsorption performance of the resultant nanomaterials were investigated in detail. The results showed that the cupric ion played an important role in the recognition of template proteins. The saturating adsorption capacity of this kind of imprinted polymers was 2.23 times greater than those of imprinted polymers without cupric ion. The imprinting factor of the imprinted materials was as high as 4.23 for the template molecule. The selective separation bovine hemoglobin from the real blood sample is successfully applied. In addition, the prepared materials had excellent stability and no obvious deterioration after five adsorption–regeneration cycles. Easy preparation, rapid separation, high binding capacity and satisfactory selectivity for the template protein make this polymer attractive in the separation of high‐abundance proteins. 相似文献
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Selective adsorption of protein by a high‐efficiency Cu2+‐cooperated magnetic imprinted nanomaterial 下载免费PDF全文
Lu Shi Yuhai Tang Yi Hao Gaiyan He Ruixia Gao Xiaoshuang Tang 《Journal of separation science》2016,39(14):2876-2883
We report a core–shell magnetic molecularly imprinted polymer with high affinity through a facile sol–gel method for the selective adsorption of bovine hemoglobin from real bovine blood. Copper ions grafted on the surface of the matrix could immobilize template protein through chelation, which greatly enhances the orderliness of imprinted cavities and affinity of polymers. The obtained products exhibit a desired level of magnetic susceptibility, resulting in the highly efficient adsorption process. The results of adsorption experiments show that the saturation adsorption capacity of imprinted products could reach 116.3 mg/g within 30 min. Meanwhile, the specific binding experiment demonstrates the high selectivity of polymers for bovine hemoglobin. Furthermore, satisfactory reusability is demonstrated by ten adsorption–desorption cycles with no obvious deterioration in binding capacity. Electrophoretic analysis suggests the polymer could be used successfully in separation and enrichment of bovine hemoglobin from the bovine blood sample, which exhibits potential application in pretreatment of proteomics. 相似文献
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Jianmin Li Lijuan Zhao Chanling Wei Zhian Sun Shanwen Zhao Tianpei Cai Bolin Gong 《Journal of separation science》2019,42(15):2491-2499
We prepared ofloxacin restricted access media molecularly imprinted polymers using surface‐initiated atom transfer radical polymerization on the surface of brominated silica gel using ofloxacin as a template molecule, methacrylic acid as a functional monomer, and ethylene glycol dimethacrylate as a crosslinking agent. We then characterized and studied the surface morphology and adsorption properties of the polymer. Experimental results show that saturation is reached within 25 min, and that the saturated adsorption capacity was 80.67 mg/g and the imprinting factor was 1.94. Our findings also showed that the polymer surface had good hydrophilicity and an excellent protein exclusion rate, which was 98.49%. The restricted access media molecularly imprinted polymers were then successfully applied to the enrichment and separation of ofloxacin in bovine serum. When combined with high‐performance liquid chromatography, and the average recovery of ofloxacin was 95.6%, and the relative standard deviation was in the range of 2.47–3.38%. In a word, the restricted access media molecularly imprinted polymers is a method that involves a simple preparation procedure that results in excellent performance, which is a great improvement in the speed of detection of antibiotics. These qualities are what bestow upon this method its great potential for broad application. 相似文献
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Synthesis of Molecularly Imprinted Polymer for Removal of Effective Impurity (Benzhydrol) from Diphenhydramine Hydrochloride Drug 下载免费PDF全文
The present study describes the synthesis and preliminary testing of molecularly imprinted polymers (MIPs) as scavenger resins for removal of the genotoxic impurities (GTI) benzhydrol from active pharmaceutical ingredients (API). A new molecularly imprinted polymer was synthesized using benzhydrol (template molecule), methacrylic acid (functional monomer), ethylene glycol dimethacrylate (cross‐linker), 2,2′‐azobisisobutironitril (intiator) and chloroform (porogenic solvent). To compare the performance of this polymer, a control polymer or non‐imprinted polymer (NIP) was prepared under the same conditions without the use of template molecule. The synthesized polymers were characterized by FT‐IR spectroscopy. Selectivity of the molecularly imprinted polymer for absorption benzhydrol impurities through adsorption experiments reviews and the results were compared with the adsorption of impurities by NIP. Various parameters were optimized such as time, pH, type of eluent for elution of impurities from polymer, concentration of sample and saturation of polymer. The proposed method was applied for removal of benzhydrol from Diphenhydramine hydrochloride syrup and passing it through the MIPs led to the quantitative removal of benzhydrol. 相似文献
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Yang Chen Xi‐Wen He Jie Mao Wen‐You Li Yu‐Kui Zhang 《Journal of separation science》2013,36(20):3449-3456
Protein‐imprinted polymers with hollow cores that have a super‐high imprinting factor were prepared by etching the core of the surface‐imprinted polymers that used silica particles as the support. Lysozyme as template was modified onto the surface of silica particles by a covalent method, and after polymerization and the removal of template molecules, channels through the polymer layer were formed, which allowed a single‐protein molecule to come into the hollow core and attach to the binding sites inside the polymer layer. The adsorption experiments demonstrated that the hollow imprinted polymers had an extremely high binding capacity and selectivity, and thus a super‐high imprinting factor was obtained. The as‐prepared imprinted polymers were used to separate the template lysozyme from egg white successfully, indicating its high selectivity and potential application in the field of separation of protein from real samples. 相似文献
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Sulfonamide imprinted polymers using co-functional monomers 总被引:1,自引:0,他引:1
Ning Zheng Yuan-Zong Li Wen-Bao ChangZong-Mu Wang Tie-Jin Li 《Analytica chimica acta》2002,452(2):277-283
Molecular imprinted polymers (MIPs) prepared using combination of acrylamide (ACM) and 4-vinylpyridine (4-Vpy) as co-functional monomers exhibited efficient recognition properties in both organic and aqueous media as HPLC stationary phase. The results indicate that amide and pyridine groups in functional monomers formed strong hydrogen-bonding interaction with the template molecule, and specific recognition sites were created within the polymer matrix during the imprinting process. When sulfamethoxazole (SMO) was used as template, a MIP prepared in a polar organic solvent (acetonitrile) using the combination of ACM and 4-Vpy showed better recognition of template than the polymer prepared in the same solvent using the combination of acidic monomer (methacrylic acid) and basic monomer 4-Vpy. On the contrary, when sulfamethazine (SMZ) was used as template, a MIP prepared using the combination of methacrylic acid (MAA) and 4-Vpy showed better recognition of template than the polymer prepared using the combination of ACM and 4-Vpy. Our results indicate that in organic media the degree of retention of the sample molecules on the imprinted polymers was controlled by the hydrogen-bonding interaction between the sample molecules and the polymer, while in aqueous media it was determined to a considerable extent by hydrophobic interactions. In both media the shape, size and the electronic structure of the template molecule were all-important factors in the recognition process. 相似文献