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灿烂甲酚蓝二聚体作为荧光探针测定人血清白蛋白 总被引:2,自引:0,他引:2
阳离子染料灿烂甲酚蓝(BCB)在适宜浓度的阴离子表面活性剂十二烷基硫酸钠(SDS)或十二烷基磺酸钠(SLS)存在时形成二聚体。研究了以此形成的现场二聚体作为荧光探针测定蛋白质的可能性。结果表明,二聚体具有弱荧光,其荧光强度的回升与体系中蛋白质的量呈线性关系,线性范围为0~7.8μg·ml-1,检出限为3.89×10-3μg·ml-1。用于人血清白蛋白的测定,结果满意。 相似文献
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在最佳实验条件下,碘苷与血清白蛋白相互作用,导致血清白蛋白的内源荧光发生特异性变化,且体系的同步荧光强度和溶液中血清白蛋白的浓度呈线性关系.据此,建立了以碘苷为荧光探针,运用固定波长同步荧光光谱分析测定人血清白蛋白和牛血清白蛋白的新方法.体系的同步荧光强度与人血清白蛋白和牛血清白蛋白分别在1.38~579 mg/L和0.78~585 mg/L范围内呈良好的线性关系,检出限分别为0.612 mg/L和0.358 mg/L.对实际样品进行回收测定,回收率为97%~101%. 相似文献
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建立了以L-Cys-CdS纳米荧光探针测定芦丁含量的荧光分析方法。以L-半胱氨酸为稳定剂,在水溶液中合成了CdS纳米荧光探针,其粒径约为20nm。芦丁对CdS纳米荧光探针有荧光猝灭作用,且其紫外光谱图与变温实验表明其为静态猝灭。考察了酸度、反应时间、干扰物质对该方法的影响。在体系pH为10.5时,该方法的线性范围为2.004~48.096μg·mL-1,r=0.9992,检出限为0.672μg·mL-1,精密度为1.2%。该方法已成功用于复方芦丁片中芦丁的含量测定,与中国药典中的标准方法比较,结果满意。该方法简便,快捷,可用于芦丁含量的测定。 相似文献
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研究了在0.1 mol·L-1 Tris-HCl缓冲溶液(pH 8.2)中,人血清白蛋白(HSA)的荧光性质以及由于苏丹红Ⅰ的存在而使HSA的荧光猝灭现象进行了研究.结果表明:当HSA溶液在280 nm波长激发时,其发射波长为337 nm.当有苏丹红Ⅰ存在时其荧光发射的强度将出现猝灭,而且荧光猝灭程度(△F)与苏丹红Ⅰ的质量浓度在0.5~7.5 mg·L-1(r=0.999 7)之间呈线性关系,方法的检出限(3Sb/k)为0.1 mg·L-1,测定限(10Sb/k)为0.5 mg·L-1,相对标准偏差(m=6,n=11)为0.7%~1.8%,加标回收率为91.9%~109.3%. 相似文献
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喹诺酮类药物对人血清白蛋白的荧光猝灭研究 总被引:11,自引:0,他引:11
观察了喹诺酮药物萘啶酸及氟哌酸对人血清白蛋白荧光的猝灭现象。根据药物对天然白蛋白及变性白蛋白荧光猝灭的不同表现,研究了萘啶酸和氟哌酸对人血清白蛋白荧光的猝灭机理,并探讨了药物与血清白蛋白的结合情况。 相似文献
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CdS quantum dots (QD) were capped with SiO2 via a microemulsion method for reducing the toxicity and imparting the biocompatibility of the CdS QD. The resulting CdS/SiO2 core/shell nanoparticles (NP) showed an improved water‐solubility and stability even in pH 4.0 acidic medium. Their fluorescence could be effectively enhanced in the presence of bovine serum albumin (BSA), due to the passivation effect of BSA on the surface of the NP. Furthermore, the concentration dependence of the fluorescence intensity obeys the Langmuir‐type binding isotherm. Thus a novel fluorescence enhancement method for the determination of BSA has been developed using the less‐toxic CdS/SiO2 core/shell NP as probes. Under optimal conditions, the linear range of calibration curve is 0.6–30 µg·mL?1, and the detection limit is 0.18 µg·mL?1. Compared with the water‐soluble CdS NP without SiO2 shell, the CdS/SiO2 core/shell NP exhibited slightly lower fluorescence response to BSA as well as other coexisting substances, such as heavy and transition metals, due to the inhibition of SiO2 shell. The proposed method was applied to the quantification of BSA in synthetic and serum samples with satisfactory results. 相似文献
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利用荧光光谱法研究了中药有效成分山豆根碱与人血清白蛋白(HSA)间的非共价结合特性。在37℃和47℃两个作用温度下,山豆根碱与HSA间的结合常数K分别为1.5671×103L.mol-1和2.4923×103L.mol-1,结合位点数n分别为0.8309和0.8630,表明温度升高有利于两者的结合。计算了热力学参数,表明该药物与蛋白的相互作用是一个熵增加和吉布斯自由能降低的自发过程,并由此推断山豆根碱与HSA之间的作用力是以疏水相互作用为主,为研究山豆根碱的药理作用和生物学效应,以及山豆根碱对蛋白质构像的影响等提供了重要信息。 相似文献
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A novel method has been developed for uric acid analysis based on the quenching of fluorescence emission from CdS quantum dots by uric acid. Also, the effect of the presence of different surfactant agents, in order to improve the fluorescent signals of the CdS QDs, has been investigated, and the cetyltrimethyl ammonium bromide (CTAB) was selected. Under optimum conditions, the calibration graph was linear over the range of 0.1 ng/mL to 12.0 ng/mL (r = 0.9950). The limit of detection (S/N = 3) was 0.1 ng/mL. The RSD for ten determinations of 5.0 ng/mL uric acid was 3.5%. The method was applied to determine uric acid in human serum and urine sample with satisfactory results. 相似文献
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CdTe/CdS量子点荧光探针测定司帕沙星含量 总被引:1,自引:1,他引:1
在水溶液中合成了巯基乙酸修饰的CdTe/CdS量子点(QDs), 基于喹诺酮类抗生素司帕沙星与CdTe/CdS量子点的荧光猝灭作用, 建立了用CdTe/CdS量子点作为荧光探针检测微量司帕沙星的新方法. 用荧光光谱、紫外光谱研究了CdTe/CdS QDs与司帕沙星的相互作用. 研究表明: 该荧光猝灭的机理属于静态猝灭, 反应的作用机理可能是司帕沙星促使QDs表面键合的有机分子发生变化, 在Cd的电子空穴上形成了碲氧复合物, 致使荧光猝灭. 实验发现, pH为6.50的磷酸缓冲溶液中, 量子点的浓度为3.75×10-4 mol/L时, 司帕沙星的浓度在0.1~50 μg/mL范围与CdTe/CdS量子点荧光猝灭强度呈良好的线性关系, 相关系数0.9992, 检出限0.01399 μg/mL. 该方法简便、快捷、灵敏、线性范围宽, 应用于司帕沙星片剂司帕沙星含量的测定, 分析结果与标示量一致; 用于牛奶中司帕沙星残留量的检测, 回收率在93.1%~102.4%, 结果满意. 相似文献
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This study was based on the use of fluorescence quenching and differentiation between static and dynamic quenching, as well as energy transfer for distance measurements. The interactions of human serum albumin with phenothiazine drugs, i.e. phenothiazine, chlorpromazine, perphenazine and promethazine, were studied and extrapolated important information on quenching mechanism, types of interaction force, binding‐site number and distance between Trp214 in HSA and the bound drugs. This study has great significance in methodology and understanding the protein‐drug interaction mechanism. 相似文献
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测定人血清白蛋白的荧光光纤流动免疫分析系统 总被引:5,自引:2,他引:3
用新型荧光光纤免疫传感流动分析测试系统,对人血清白蛋白(HSA)进行测试;将偶联于微晶纤维素表面形成的HSA固相抗体与待测HSA(抗原)、荧光标记抗原(FTTC-HSA)竞争性结合。经稀碱液处理,固相载体与抗原抗体复合物分离,用新型荧光光纤免疫传感流动分析系统测定解析液的荧光值,以求得待测HSA的含量;该法检出限为0.1g/L,日内RSD0.91%-6.4%,日间RSD1.8%-8.0%,加标回收率91%-120%;用该法测定注射用HSA,与临床检验常用的溴甲酚绿化对照,具有良好的相关性(r=0.9808)。 相似文献