芯片式流通池顺序注射可更新表面反射光谱法用于酶反应检测

将芯片式流通池顺序注射可更新表面反射光谱法用于酶反应检测。HRP催化H2O2氧化BPR底物的反应用于对H2O2的检测。此反应体系与葡萄糖氧化酶联用，用于对血清中葡萄糖的检测。     

芯片式流通池顺序注射化学发光法测定水中的亚硝酸根

微流控芯片(Microfluidic chips)是微全分析系统(μTAS)研究中最为活跃的领域,在仪器微型化方面展现出很多的优点^[1].化学发光由于其自身的特性在微芯片检测中应用逐渐增多^[2,3].     

可更新表面分析技术进展

概述了流动注射及顺序注射可更新表面分析技术及仪器的进展。介绍了该技术原理、仪器系统、流通池等方面的新发展以及在传感器、免疫分析、生物配位作用检测、酶反应检测、细胞功能检测等领域的应用。参考文献32篇。     

芯片式流通池用于顺序注射可更新表面荧光免疫法测定人血清中的IgG

采用芯片式流通池作为非均相免疫反应和原位固相荧光检测的场所,用双岔光纤将芯片式流通池与荧光光度计耦联,以双抗夹心式非均相免疫反应的模式,研究建立了测定人血清中IgG的顺序注射可更新表面非均相免疫分析新方法.     

流动注射-可更新表面技术进展

概述了流动注射－可更新表面技术的进展。介绍了该技术的原理、仪器发展及其在免疫分析、生物配位作用检测、细胞功能检测、分离与预浓集等领域的应用。参考文献27篇。     

顺序注射可更新表面固相荧光免疫法测定人血清中免疫球蛋白G

建立了采用芯片式微型流通池的测定人血清中免疫球蛋白G(IgG)的顺序注射可更新表面非均相荧光免疫分析法。将羊抗人IgG抗体固定于包被有蛋白A的Sephamse CL4B凝胶微珠,然后制备成固相抗体。用标记FITC的抗人IgG抗体作为第二抗体。固相抗体、血清试样和荧光标记第二抗体由顺序注射系统注入芯片式微型流通池,并在其中进行免疫反应生成夹心式抗体．抗原荧光复合物。荧光分光光度计通过光纤与流通池耦合测定截留于流通池中的抗体一抗原复合物荧光强度。一次测定完成后,微珠即被排出流通池。流通池经缓冲液清洗后即可进行下一次测定。体系经优化后,检出限为0．1mg／L IgG,分析速率达到11次／h。3．9mg／L IgG的日内和日间精密度(RSD)分别为1．7％和5．2％;校正曲线的线性范围为0．3—7．0mg／L IgG。所建立的方法已成功地应用于人血清中IgG的测定。     

固相表面荧光光谱法在药物分析中的应用

本文综述了近十年来固相表面荧光光谱法、固相萃取-固相表面荧光联用技术以及流动注射分析、顺序注射分析和可更新表面技术与固相表面荧光联用技术在药物分析中的应用.展望了固相表面荧光光谱法在药物分析中的发展趋势和前景.     

Flow and Sequential Injection Manifolds for the Spectrophotometric Determination of Captopril Based on its Oxidation by Fe(III)

 Two new simple and rapid methods are reported for the accurate and precise spectrophotometric determination of captopril (CPL) using flow (FI) and sequential injection (SI) analysis. The methods are based on the fast oxidation of CPL by Fe(III). The produced Fe(II) reacts with 2,2′-dipyridyl-2-pyridylhydrazone (DPPH) in acidic medium to form a colored complex which is monitored spectrophotometrically at 535 nm. Both methods allow the determination of the analyte up to 1000 mg L⁻¹ at a sampling rate of 120 and 60 injections per hour for FI and SI, respectively. The methods are very precise [s _r=0.8 and 1.2% at 500 mg L⁻¹ CPL (n=12) for FI and SI, respectively] and the 3σ detection limits (c _L=4.0 and 7.0 mg L¹, respectively) are quite satisfactory. Their application to a variety of anti-hypertensive commercial pharmaceutical formulations showed excellent results (relative errors, e _r, < ± 1.6% in all cases compared to an official HPLC method), while common pharmaceutical excipients were found not to interfere. Recovery experiments further verified the accuracy of the developed methods, as the percent recoveries were in the range of 98.1–102.5%. Author for correspondence. E-mail: themelis@chem.auth.gr Received May 9, 2002; accepted January 8, 2003 Published online May 5, 2003     

Determination of Orciprenaline Using a Flow Injection Analysis System with Sequential Potentiometric and Spectrophotometric Detection

Abstract

This work describes an attempt to have a flow injection analysis (FIA) system for Orciprenaline with potentiometric and spectrophotometric detectors working sequentially. The potentiometric detection was performed using an orciprenaline ion-selective electrode made of orciprenaline ion-associate with phosphotungstic acid incorporated in a PVC matrix membrane, followed by sequential spectrophotometric detection of the same sample using the reaction of orciprenaline with phosphomolybdic acid in alkaline medium and measurement at 670 nm using a USB2000 fiber-optic spectrophotometer. The method was applied and validated for the assay of different samples that are 1.0 × 10^?2–1.0 × 10^?7 M orciprenaline, and the recovery values for Alupent® tablets, plasma and urine sample ranged from 99.39–100.93, 99.87–100.57, and 98.83–100.64 respectively for the potentiometric detector and 99.66–100.58, 99.78–100.69 and 99.12–100.92 respectively for the sequential spectrophotometric detector. It was found that using the double detection system compensated for both the unselectivity of the spectrophotometric method and the low detection limit of the potentiometric method (6.3 × 10^?4 M). Although two detectors were used in the measurements, the method is still very simple to design and apply, in addition to being rapid and less expensive than other more sophisticated techniques applied in the literature and can therefore be used for other pharmaceutical compounds as well.     

在线自发电源激发的流动注射电致化学发光测定异烟肼

采用了一种与流动注射体系组合的在线自发电池作为电致化学发光的激发源（ 简称FI－GCECL）。实验发现在碱性介质中，Al/Ag双金属对可以提供稳定的电位输 出并可激发鲁米诺在铂电极表面的电致化学发光。此自发电池具有使用寿命长、电 位可由碱性介质组成调节、可组合到流动注射体系中等特点，利用这种组合体系， 检测了三家厂生产的片剂中的异烟肼含量，将结果与药典标准方法进行了比较，证 明了这种电致发光分析的可行性。     

Automated Determination of Hydrogen Peroxide at the Micro-Molar Level in Rainwater and Snow Using a Stopped-Flow Approach in a Hybrid Sequential Injection/Flow Injection Manifold

A new automated method is reported for the determination of H₂O₂ in real samples. The method is based on the quenching effect of the analyte on the reaction between tris(2-carboxyethyl)phosphine (TCEP) and Ellman's reagent (DTNB). All necessary steps were accomplished under flow conditions using a hybrid sequential injection (SI)/flow injection (FI) setup. The sensitivity was enhanced by applying a stopped-flow step (120 s) in order to promote the reaction between H₂O₂ and TCEP. The proposed analytical protocol was validated for linearity (10–75 µmol L^?1), limits of detection (c _L = 1.0 µmol L^?1), quantitation (c _Q = 3.3 µmol L^?1), precision (s _r = 1.3–1.7%), accuracy, and selectivity. It was then applied successfully to the analysis of H₂O₂ in spiked rainwater and snow samples.     

Catalytic Determination of Trace Formaldehyde with a Flow Injection System Using the Indicator Reaction between Crystal Violet and Bromate

A sensitive flow injection method is proposed for the determination of formaldehyde based on its catalytic action upon the redox reaction between crystal violet and potassium bromate in phosphoric acid medium. The reaction is monitored spectrophotometrically by measuring the decrease in absorbance of crystal violet injected at the maximum absorption wavelength of 600nm. A calibration graph from 0.018 to 1.400µgmL^–1 is obtained, and the detection limit is 0.007µgmL^–1 in a sample volume of 30µL. Up to 15 samples can be analyzed per hour with a relative standard deviation of 1.2 and 2.3%, respectively, for the determination of 1.0 and 0.1µgmL^–1 of formaldehyde. The method is free from most interference. This method has been applied to the determination of formaldehyde in air, decoration boards and paints, and the results compare well with those provided by iodimetry.