共查询到20条相似文献,搜索用时 62 毫秒
1.
M. El-Tawoosy N. Farouk A. S. El-Bayoumy 《Journal of Radioanalytical and Nuclear Chemistry》2011,290(3):595-600
An adopted method for the preparation of high radiochemical purity 125I-atenolol was investigated. Direct radioiodination of atenolol was carried out using N-bromosuccinamide or hydrogen peroxide as an oxidizing agent. The reaction proceeds well within 30 min at room temperature
(25 ± 1 °C) and afforded a radiochemical yield up to 97% as pure as 125I-atenolol. Different chromatographic techniques (electrophoresis, TLC and HPLC) were used to determine the radiochemical
yield and purity of the labeled product. Biodistribution studies were carried out in normal Albino Swiss mice and the results
indicate that 125I-atenolol can be used safely as myocardial imaging agent. 相似文献
2.
K. M. El-Azony 《Journal of Radioanalytical and Nuclear Chemistry》2010,284(2):315-320
A preparation of 125I-celecoxib is carried out via an electrophilic substitution reaction. The reaction parameters studied were celecoxib concentration,
reaction temperature, pH of the reaction mixture and kinds of oxidizing agents in order to obtain a high radiochemical yield
of the 125I-celecoxib. Using 3.7 MBq of Na125I, 150 μg (3.9 mM (mmol/L)) of celecoxib, and 1.6 mM (mmol/L) of chloramine-T (CAT) as oxidant at pH 4 and 60 °C for 15 min
a maximum radiochemical yield of 125I-celecoxib (65%) was obtained. The labeled compound was separated and purified by means of high pressure liquid chromatography
(HPLC). The biological distribution in infected mice indicates the suitability of radioiodinated celecoxib as imaging of tumor. 相似文献
3.
Kh. M. Sallam H. M. Abdel-Aziz K. A. Moustafa T. Siyam 《Journal of Radioanalytical and Nuclear Chemistry》2010,284(1):29-35
The aim of the present study is to develop of chromatographic separation of some biological materials using poly(acrylamide-acrylic
acid-dimethylaminoethyl methacrylate) resin P(AAm-AA-DMAEMA). The copolymer was prepared by a template copolymerization of
DMAEMA and AA in aqueous solution on polyacrylamide (PAAm) as a template polymer and N,N-methylenebisacrylamide (NMBA) as a crosslinker using gamma rays as initiator. Some biological materials such as alpha-fetoprotein
(AFP), bovine serum albumin (BSA) and tri-iodothyronine (T3) were labeled using 125I and the purifications of all tracers were carried out using prepared resin compared with Sephadex. Factors affecting were
studied to reach the most efficiency purification including pH buffer, variable elution volumes, flow rate and temperature.
The radiochemical purity percent was determined using paper electrophoresis and immunoreactivity of tracer was checked. The
efficiency of purification that obtained using the prepared resin is nearly equivalent to that of Sephadex. 相似文献
4.
Y. M. Efimova B. Wierczinski S. Haemers A. A. van Well 《Journal of Radioanalytical and Nuclear Chemistry》2005,264(1):91-96
Summary Bovine serum albumin (BSA) and lysozyme (LSZ) were radiolabeled with 125I. Three different methods for protein iodination with 125I were optimized. Parameters like incubation time and ratio of oxidizing agent and amount of protein were established. During protein iodination with 125I, structural damages caused by the introduction of iodine into the protein may occur. These damages depend on the oxidizing agent used and may lead to considerable changes in the protein structure and, hence, their biological activity. Changes in secondary structure of LSZ and BSA were examined by circular dichroism (CD). Enzymatic activity tests were performed with lysozyme to check its biological activity. The Iodo Bead was found the best oxidizing agent for protein iodination. 相似文献
5.
Ethyl-substituted bis-triazinylpyridine (Et-BTP), a nitrogen containing soft-donor extractant, was used in investigations
pertaining to the separation of Am3+ and Eu3+ from dilute nitric acid feed solutions by extraction chromatography using XAD-4 as the inert support, chlorinated dicarbollide
as the modifier and 2-nitrophenyloctylether (NPOE) as the diluent. After carrying out a series of experiments, the optimum
composition of the extractant mixture for the resin was found out to be 0.1 M Et-BTP and 0.025 M CCD in NPOE. Separation factor
values were encouraging to carry out subsequent batch uptake studies at varying nitrate ion concentration which indicated
favourable separation behaviour up to NaNO3 concentration of 2 M. Column studies have been carried out and conditions for elution and separation of Am3+ from Eu3+ have been found out. Long term stability of the resin was also investigated. 相似文献
6.
Adsorption of long-lived 125Sb radioisotope (T
1/2 = 2.75 y) on alumina (Al2O3) and titania (TiO2) has been studied at different pH. Both the oxides have good adsorption capability for the 125Sb radioisotopes but the TiO2 is much superior. Adsorption kinetics of 125Sb radioisotopes on TiO2 surface and desorption of 125Sb radioisotopes from TiO2 surface in acidic and alkaline media have also been studied. The 125Sb-TiO2 phase has been subjected to γ-irradiation and found to be radiation stable against antimony release. 相似文献
7.
Chang Heon Lee Myung Ho Lee Sun Ho Han Yeoung-Keong Ha Kyuseok-Song 《Journal of Radioanalytical and Nuclear Chemistry》2011,288(2):319-325
A simple and rapid separation procedure was systemized for the determination of 99Tc, 90Sr, 94Nb, 55Fe and 59,63Ni in low and intermediate level radioactive wastes. The integrated procedure involves precipitation, anion exchange and extraction
chromatography for the separation and purification of individual radionuclide from sample matrix elements and from other radionuclides.
After separating Re (as a surrogate of 99Tc) on an anion change resin column, Sr, Nb, Fe and Ni were sequentially separated as follows; Sr was separated as Sr (Ca-oxalate)
co-precipitates from Nb, Fe and Ni followed by purification using Sr-Spec extraction chromatographic resin. Nb was separated
from Fe and Ni by anion exchange chromatography. Fe was separated from Ni by anion exchange chromatography. Ni was separated
as Ni-dimethylglyoxime precipitates after the removal of 134,137Cs and 110mAg by Cs-phosphotungstate and AgCl precipitation, respectively. Finally, the radionuclide sources were prepared by precipitation
for their radioactivity measurements. The reliability of the procedure was evaluated by measuring the recovery of chemical
carriers added to a synthetic radioactive waste solution. 相似文献
8.
Revital Sasson Dan Vaknin Avihai Bross Efraim Lavie 《Journal of Radioanalytical and Nuclear Chemistry》2010,283(3):753-756
A practical and reliable HPLC method was used for the determination of 2-[4-N-(2-hydroxyethyl)-1-piperazinyl]-N′-ethanesulfonic acid (HEPES) content in the 68Ga-labeled [1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid]-1-Nal3-octreotide (DOTANOC). Linearity of this method
was observed in a concentration range of 0.01–10 mg mL−1 and the quantitative limit (signal to noise = 11) was determined as 10 μg mL−1. The HEPES concentration in the final products of 68Ga-DOTANOC was typically lower than the detection limit. Pure water and HEPES buffer as reaction medium were investigated
using various activities of gallium-68. It was demonstrated that the presence of HEPES buffer consistently furnished very
high radiochemical purity of 68Ga-DOTANOC, which remained stable for several hours post-labeling. Evidence is provided that in addition to its role as a
buffer, HEPES also functions as a radioprotectant agent. 相似文献
9.
N. Farouk M. A. Motaleb H. A. Shweeta K. Farah M. T. Kolaly 《Journal of Radioanalytical and Nuclear Chemistry》2005,266(3):405-410
Summary [125I]iodepidepride, (s)-(-)-[(1-ethyl-2-pyrrolidinyl)methyl]-5-[125I]-iodo-2,3-dimethoxybenzamide is the iodine substituted analogue of isoremoxipride, both of which are very potent dopamine
D2-antagonists. Epidepride was radioiodinated using different oxidizing agents such as chloramine-T, iodogen, iodogen glass
frit and hydrogen peroxide. Chloramine-T is a powerful oxidizing agent compared to both iodogen and hydrogen peroxide so that
the side products, especially the chlorinated epidepride, decreases the radiochemical yield. This chlorinated epidepride is
minimized in the case of iodogen and iodogen glass frit and are not observed in case of the non-chlorinated oxidizing agent
hydrogen peroxide. TLC and HPLC were used to analyze the reaction components and to estimate both the radiochemical yield
and purity. The reaction parameters such as reaction time, pH, epidepride and oxidizing agent concentrations and the stabilty
of the final product were studied to optimize the radiochemical yield and purity. The optimized radiochemical yield was about
90% and the radiochemical purity of the final product was 99.9%. 相似文献
10.
Veronika Drábová Jana Strišovská Lívia Dvorská Jozef Kuruc 《Journal of Radioanalytical and Nuclear Chemistry》2012,292(3):1141-1145
Two separation techniques for strontium determination using AnaLig® Sr01 molecular recognition technology and extraction chromatography Sr® resin were tested. The methods performance was investigated by analysis of NPL (High Alpha–Beta 2003) intercomparison sample. The results obtained for both procedures were compared in terms of activities and recoveries. Data analysis proved a good agreement with the reference values. The AnaLig® Sr01 separation method for 90Sr determination was successfully validated with the same performance as the Sr® resin method. 相似文献
11.
K. F. Allan M. M. S. Ali H. A. Hanafi K. M. El-Azony 《Journal of Radioanalytical and Nuclear Chemistry》2010,284(2):297-301
The activated carbon was prepared by using corncobs and characterized by sorpatometer for using as an exchanger material to
separate the generated 113mIn from 113Sn and 124,125Sb. To optimize the separation process, the different parameters like acetone percentage, HCl concentration were studied.
The exchange capacity of Sn(IV) is 7.6 meq/g onto the activated carbon and the elution efficiency of 113mIn > 80% by using 10 mL of 0.2 M HCl-80% acetone with flow rate 1 mL/min. The radionuclidic purity and radiochemical purity
of the eluted 113mIn were examined and clarified the presence of 124,125Sb with relatively high level as radio impurities, so further separation was carried out by using Dowex 1×8 as an anion exchanger
below the activated carbon matrix on the same separation column to adsorb the 113Sn and 124,125Sb, which escape from the activated carbon matrix. 相似文献
12.
H. Aglan S. A. Kandil H. A. Hanafi M. A. Mousa Z. A. Saleh 《Journal of Radioanalytical and Nuclear Chemistry》2009,280(3):533-538
The radiochemical separation of no-carrier-added cerium from proton irradiated lanthanum was studied by solvent extraction
using DEE, TBP and TPPO, the latter reagent being employed for the first time for separation of radiocerium from bulk of lanthanum.
Distribution coefficients of cerium and lanthanum were investigated as a function of equilibrium time and concentration of
HNO3. A mixture of 0.05M K2Cr2O7 and 0.1M H2SO4 was used as an oxidizing agent to improve the separation efficiency of cerium. A comparative study of the three extractants
released that DEE is the best for separation of cerium from bulk of lanthanum oxide. The target was prepared by pressing.
The production of 139Ce of high radionuclidic purity and chemical purity via irradiation of lanthanum oxide target at MGC-20 cyclotron with protons
of energy 14.5 MeV is described. The experimental yield was found to be 153 kBq/μA·h. 相似文献
13.
Judit Groska Zsuzsa Molnár Edit Bokori Nóra Vajda 《Journal of Radioanalytical and Nuclear Chemistry》2012,291(3):707-715
The presence of 89Sr and 90Sr in the biosphere constitutes a biological hazard. There are several analytical methods for the determination of 89Sr and 90Sr. Three analytical methods of various application fields using selective Sr resin for Sr separation and DGA resin for Y
separation and measuring techniques, i.e. liquid scintillation spectrometry and Cerenkov counting are discussed in the paper.
The calculation techniques are compared in the aspects such as trueness and accuracy of the results and the limit of detection.
Uncertainties and detection limits are calculated using the spreadsheet method. 相似文献
14.
Chang Heon Lee Moo Yul Suh Kwang Yong Jee Won Ho Kim 《Journal of Radioanalytical and Nuclear Chemistry》2007,272(1):187-194
A sequential separation procedure has been developed for the determination of 99Tc, 94Nb, 55Fe, 90Sr and 59/63Ni in various radioactive wastes generated from nuclear power plants. Ion exchange and extraction chromatography were adopted
for individual separation of the radionuclides. Precipitation was supplementarily utilized for both purification of the individual
radionuclides and preparation of the radionuclide sources for use in a radioactivity measurement. The chromatographic separation
behavior of the radionuclides both from the sample matrix metals and from one another was investigated using stable metals,
Re (as a surrogate of 99Tc), Nb, Fe, Sr and Ni. The validity of the procedure for reliability and applicability was evaluated by measuring the recovery
of the metal carriers added to synthetic radioactive waste solutions. The recoveries by the chromatographic separation were
in the range of 84.8 to 102.2% with 2s of less than 8.6%, the recoveries by the precipitation being in the range of 84.3 to 97.3% with 2s of less than 10.9%. 相似文献
15.
C. Dirks B. Scholten S. Happel A. Zulauf A. Bombard H. Jungclas 《Journal of Radioanalytical and Nuclear Chemistry》2010,286(3):671-674
Cu isotopes (e.g. 64Cu) increasingly find use in radiopharmaceutical applications, accordingly fast and reliable methods for the production of
these isotopes are needed. The aim of the presented project is the characterization of a Cu selective extraction chromatographic
resin for the fast and selective separation of Cu radionuclides, e.g. from irradiated targets. The characterisation of the
resin includes the determination of weight distribution factors D
w
of Cu, Ni, Zn and other potentially interfering elements and impurities for varying acids and pH values, the influence of
macro amounts of Ni and Zn on the extraction of Cu as well as the influence of other potential interferents. Based on the
obtained results, a method for the separation of Cu and its purification from irradiated Ni or Zn targets was developed and
tested on simulated Ni and Zn targets. 相似文献
16.
I. I. Dovhyi D. A. Kremenchutskii V. Yu. Proskurnin O. N. Kozlovskaya 《Journal of Radioanalytical and Nuclear Chemistry》2017,311(3):1643-1648
90Y was separated from 90Sr using an extraction chromatographic resin consisting of 4, 4′(5′)-bis-t-butylcyclohexano-18-crown-6 (DtBuCH18C6), 1-ethyl-3-methylimidazolium bis(trifluoromethanesulfonyl) amide (C2mimNTf2), and a polymer (Amberlite XAD-7). Ionic liquid was introduced into the column to improve the separation efficiency. The column showed an excellent performance for the separation of Y from Sr. After the separation, the ratio of 90Sr/90Y was <2.0 × 10?5; the column was recycled for >18 times. This study provides preliminary results on columns to produce 90Y with a high purity in radiopharmaceuticals. 相似文献
17.
Rajesh Chakraborty Sanjoy Dhara Sukalyan Basu Pabitra Chattopadhyay 《Journal of Radioanalytical and Nuclear Chemistry》2011,287(1):55-59
An inorganic ion exchanger, quinolinephosphomolybdate has been synthesized and characterized by elemental analysis, infrared
(IR) and X-ray diffraction (XRD) spectroscopy. This compound is highly stable toward thermal, chemical and radiation dose.
This has been employed in the separation of carrier-free 90Y from its parent 90Sr from an equilibrium mixture. The absorbed daughter was recovered by using 0.0284 mol L−1 ascorbic acid solutions at pH 5.0 as eluting agent. 相似文献
18.
M. Javed I. U. Khan M. Naeem S. Asghar H. Naseer M. S. Iqbal J. Anwar 《Journal of Radioanalytical and Nuclear Chemistry》2008,277(2):303-309
A tetradentate set of N2O2 salicylaldehyde-amine-N-dione Schiff base was prepared by condensation with salicylaldehyde, ethylenediamine, 2,4-dione and
reduction with NaBH4. The ligand system was characterized by 1H-NMR and FT-IR spectroscopy and HPLC. Radiolabeling studies of the 99mTc-complex were performed using stannous ions as the reducing agent. The purity of the complex was determined by ascending
solvent system on paper chromatography and instant thin-layer chromatography (ITLC). The yield of the complex was >90%. Biodistribution
of the 99mTc-complex of the precursor was studied in rabbits. A significant uptake and retention of injected activity was observed in
the liver and cleared through the bladder. A faint activity was also observed in kidneys. These results indicate that the
proposed system may be suitable for development of a liver/spleen imaging agent for future clinical applications. 相似文献
19.
Electrochemical copolymerization of N
1,N
3-bis(thiophene-3-ylmethylene)benzene-1,3-diamine (TMBA) with 3,4-ethylenedioxythiophene (EDOT) was carried out in a CH3CN/LiClO4 (0.1 M) solvent-electrolyte via potentiodynamic electrolysis. Chemical structure of the monomer was determined by nuclear
magnetic resonance (1H NMR) and Fourier transform infrared (FTIR) spectroscopy. The resulting copolymer was characterized by cyclic voltammetry
(CV), FTIR, scanning electron microscopy (SEM), and thermogravimetry analyses (TGA). Conductivity measurements of the copolymer
and PEDOT (poly(3,4-ethylenedioxythiophene)) were carried out by the four-probe technique. 相似文献
20.
Binita Dutta Moumita Maiti Susanta Lahiri 《Journal of Radioanalytical and Nuclear Chemistry》2009,281(3):663-667
natY foil was irradiated by 20 MeV proton to produce no-carrier-added 88,89Zr. A comparative evaluation on radioanalytical separation methods of 88,89Zr was carried out from irradiated target matrix by both liquid–liquid (LLX) and solid–liquid (SLX) extraction methods using
di-(2-ethylhexyl) phosphoric acid (HDEHP) dissolved in cyclohexane as liquid cation exchanger and Dowex 50W-X8 H+ form (20–50 mesh) as solid cation exchanger. Both the methods offer good separation and high yield of nca 88,89Zr but SLX offers much higher separation factor and better yield. 相似文献