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Xiao Yan Zhang Quan Ling Suo Xiong Hui Wei Department of Applied Chemistry College of Chemistry & Molecular Engineering Peking University Beijing China Department of Chemical Engineering Inner Mongolia University of Technology Huhhot China 《中国化学快报》2007,18(6):722-725
To study the possible anticancer mechanisms of chelerythrine (CHE), and its interactions with cytidine were investigated by UV-vis spectrophotometric and spectrofluorimetric measurements and by thermodynamic calculations. The binding of CHE to cytidine could be characterized by the hypochromic and bathochromic effects in the absorption bands, and the quenching of fluorescence intensity. The spectral data were fit by linear analysis, yielding a binding constant of 2.49×104 L mol-1 at 25℃of CHE and cytidine, and a van't Hoff enthalpy of -20.02 kJ/mol for the exothermic interaction in the standard state. In addition, with 相似文献
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