高灵敏度双介体基胆固醇生物传感器的研制

研制了一种高灵敏度、高准确性、微小取样（－滴血,Ｖ≥２５μＬ）并可快速测量（≤６０ｓ）的实用型夹心式胆固醇生物传感器。它是由双介体、三酶生化氧还体系构成的电流型传感器。其中,第一介体含于敏感电极石墨电导层内,第二介体及三种酶、相试剂含于试剂纤维层中。本传感器可用来直接测量从正常人到异常人的血胆固醇含量（２～１０ｍｍｏｌ／Ｌ）,并将实现商品化。     

胆固醇电化学生物传感器的研究与进展

胆固醇作为一些疾病的重要生物标志物,开发准确且快速的胆固醇检测技术在生物医学应用中具有重要意义.电化学生物传感技术具有检测速度快、灵敏度高、操作方便等优点,成为研制新型高性能胆固醇传感器的主要研究方向.近年来,材料合成技术的提高,新型材料种类的丰富,促进了胆固醇电化学生物传感器在脑和心脏血管疾病临床分析中蓬勃发展.本文...     

以聚丙烯酰胺凝胶为基质的光导纤维pH传感器

以凝胶为基质的新型固定化试剂制备了3种pH荧光传感器.在实验条件下,其信号响应时间低于10s,pH突跃范围为1.83～7.54和1.39～2.97.基本上无试剂脱落,在试剂本身化学稳定期限内,具有一定的使用寿命.     

苦杏仁甙化学发光生物传感器

将具有分子识别功能的β－葡萄糖甙酶和能进行换能反应的Ｌｕｍｉｎｏｌ分别固定在壳质胺和大孔阴离子交换剂的柱中,组成流动注射系统。苦杏仁甙在β－葡萄糖甙酶催化下分解生成的ＣＮ＾－（分子识别反应）与溶解氧反应生成超氧阴离子自由基,继而同Ｌｕｎｉｎｌ反应产生化学发光（换能反应）。这一新型生物传感器的化学发光强度与苦杏杜甙量在１～２００ｕｇ之间呈良好线性关系,检出限为０．３ｕｇ,相对标准偏差为３．１％,并具     

化学发光生物传感器的研究进展

简要介绍了化学发光生物传感器在近几年取得的重要研究成果；内容主要化学发光生物传感器研究中生物组分的固定化方法及生物传感器在分析化学中的应用，引用参考文献49篇。     

用聚乙烯醇凝胶为基质的光导纤维铝传感器的研究

本文将桑色素通过氰脲酰氯固定在聚乙烯醇上,以戊二醛作为交联剂,在光导纤维上制得对铝离子产生荧光响应的聚乙烯醇凝胶传感膜,这种光纤传感器对铝离子的响应时间在10s以内,在3×10^-7～10^-4mol/L铝浓度范围内有良好的线性关系,实验结果与理论式相吻合。     

壳聚糖固定双酶的丝网印刷胆固醇传感器的制备及应用

以亚甲基蓝为电子媒介,通过壳聚糖固定胆固醇氧化酶和辣根过氧化酶在丝网印刷电极表面,制成了一种新型胆固醇生物传感器,实现了低电位下对胆固醇的间接测定.循环伏安法和恒电位法用于研究修饰电极的电化学特性,在优化的试验条件下,安培法对胆固醇响应的线性范围为2.0×10-5～3.0×10-3mol·L-1,检出限(3S/N)为5.0×10-6mol·L-1.用同一支电极对6.0×10-4mol·L-1胆固醇溶液进行10次连续测定,测定值的相对标准偏差为4.6%,用3支电极做相同试验,测得相对标准偏差为6.2%.应用此传感器测定了血清样品中胆固醇含量,测定值的相对标准偏差(n=5)以及回收率试验结果依次在2.3%～6.1%之间及94.3%～105.2%之间.     

A Novel Fiber Optic Biosensor for the Determination of Adrenaline Based on Immobilized Laccase Catalysis

Abstract

A novel fiber optic biosensor for the determination of adrenaline based on immobilized laccase catalysis and fluorescence quenching was designed and fabricated. The immobilized laccase formed by the immobilization of laccase on the CuTAPc-Fe₃O₄ nanoparticles composite were used to catalyze the oxidation of adrenaline and the fluorescent oxygen-sensing membrane was used to detect the consumption of oxygen. The effects of pH and temperature on laccase activity using adrenaline as the substrate were studied. The optimal pH and temperature for the activity of immobilized laccase are 5.0 and 55°C, respectively. The immobilized laccase has good thermal, storage and operation stability. The lock-in technology was used to detect the change of the life time of the oxygen-sensing membrane. By using ABTS as the electron mediator, the biosensor showed a response time of 30 sec. The biosensor has good performance in the adrenaline concentration ranges of 2.0 × 10^?7 to 9.0 × 10^?7 mol/l and 1.0 × 10^?8 to 9.0 × 10^?8 mol/l, and it also shows good stability.     

A Fiber Optic Lactate Biosensor with an Oxygen Optrode as the Transducer

Abstract

A biosensor for continuous determination of lactate is presented. Lactate monooxygenase was immobilized covalently on nylon membranes, and the consumption of oxygen was measured by following, via a fiber optic bundle, the changes in the fluorescence of an oxygen-sensitive dye dissolved in 10- and 25-um silicone membranes placed beneath the enzyme layer. Oxygen is consumed as a result of the oxidation of lactate by the enzyme, and the decrease in its partial pressure is indicated by the fluorescent dye. For two types of sensors (with different nylon membranes and different thicknesses of the indicator layer) the analytical ranges were 2–50 mM and 0.3–6.0 mM, with response times (t₉₀) of 2.3–3.0 and 4.0–6.0 min, respectively.     

菲涅耳反射型光纤生物传感器研究

构建了一种基于菲涅耳反射原理的光纤生物传感器,以生物素-链霉亲和素体系为模型验证了其进行生物传感的可行性.首先,利用硅烷偶联剂,在切平整的普通单模光纤端面修饰上生物素,将其作为吸附链霉亲和素的生物敏感膜.然后,将光纤端面浸入不同浓度的链霉亲和素溶液中,测量宽带光源经过光纤端面反射后的光谱.实验结果表明,反射光谱的相对功率与链霉亲和素溶液浓度之间呈线性关系,测量得到灵敏度为15.38 dB/(mg/mL).用牛血清白蛋白代替链霉亲和素,测量得到的反射光谱曲线与在之前浸入生物素后的反射光谱曲线基本重合,证明此生物传感器的选择性好.最后以人免疫球蛋白G与山羊抗人免疫球蛋白G体系验证了此结构进行生物传感的实用性.     

光纤化学发光和生物发光传感器

本文综述国内外有关光纤华东安光和生物发光传感器的研究成果，涉及传感器的种类传感层的制作方法及传感器的设计等。     

基于蛋白质直接电子转移的全胆固醇传感器

以壳聚糖为载体, 将血红蛋白、胆固醇氧化酶及胆固醇酯酶固定在玻碳电极表面, 在不使用任何电子媒介体的条件下, 利用血红蛋白和电极之间的直接电子转移, 制备出高选择性的全胆固醇生物传感器, 并用于测定血清中总胆固醇含量. 利用循环伏安法和恒电位法研究了该传感器的电化学特性. 在优化的实验条件下, 该传感器对胆固醇响应的线性范围为10~110 mg/dL, 检出限为5 mg/dL(信噪比的3倍), 响应时间为60 s. 对人血清中总胆固醇的测定表明, RSD小于6.2%, 回收率为95%~106%.     

金属酞菁仿生催化儿茶酚胺氧化性能研究及其用于肾上腺素浓度的光学检测(英文)

采用电子吸收光谱法研究了5种金属酞菁MPcs(M=Mn(Ⅱ),Fe(Ⅱ),Ni(Ⅱ),Cu(Ⅱ))仿生催化肾上腺素(Adrenaline,AD)和去甲肾上腺素(Noradrenaline,NA)2种儿茶酚胺的氧化性质,相应的氧化产物分别为三羟基-N-甲基-吲哚和三羟基-吲哚。用氧化产物的特征吸收峰强度评价金属酞菁的催化能力,实验表明,在最佳催化条件下,金属酞菁催化效率有以下顺序ηMnPcηFePcηNiPcηCuPcηCoPc。以酞菁锰仿生酶为催化剂,采用锁相放大技术构建了一种新型光纤生物传感器实现对肾上腺素浓度的测定,系统地研究了光纤肾上腺素传感器的性质:在2.0×10-6~9.0×10-5mol·L-1范围,滞后相移φ与肾上腺素的浓度有较好的线性关系,检测下限为4.0×10-7mol·L-1,响应时间为10min,该传感器有良好地重复性和稳定性。     

Direct Electrochemistry of Cholesterol Oxidase Immobilized on a Conducting Polymer: Application for a Cholesterol Biosensor

Direct electrochemistry of cholesterol oxidase (ChOx) immobilized on the conductive poly‐3′,4′‐diamine‐2,2′,5′,2″‐terthiophene (PDATT) was achieved and used to create a cholesterol biosensor. A well‐defined redox peak was observed, corresponding to the direct electron transfer of the FAD/FADH₂ of ChOx, and the rate constant (k_s) was determined to be 0.75 s^?1. Glutathione (GSH) covalently bonded with PDATT was used as a matrix for conjugating AuNPs, ChOx, and MP, simultaneously. MP co‐immobilized with ChOx on the AuNPs‐GSH/PDATT exhibited an excellent amperometric response to cholesterol. The dynamic range was from 10 to 130 μM with a detection limit of 0.3±0.04 μM.     

Voltammetric Sensor for Total Cholesterol Determination

We report on a voltammetric sensor for the detection of total cholesterol. The sensor was fabricated by co-immobilization of two enzymes: cholesterol oxidase (ChOx) and horseradish peroxidase (HRP) on porous graphite. The electrochemical behavior of the sensor was studied with the use of linear sweep voltammetry. It has been shown that the sensor has high stability and high sensitivity (16 μA mM⁻¹ cm⁻²). The biosensor exhibited a wide linear range up to 300 mol/dm³ in a condition close to physiological (pH=6.86). Besides, the interferences of some key analytes containing in the blood were studied. As a matter of fact, making a fabricated sensor is rather promising for using in clinical practice.