Evaluation of the radiation dose to a phantom for various X-ray exposure factors performed using the dose area product in digital radiography

The aim of this study is to measure the dose area product (DAP) in digital radiography by using a DAP meter to determine the X-ray exposure. Pediatric X-ray examinations can be obtained for any radiographic examinations using the selected radiographic examination parameters (kVp and mAs), the DAP information recorded. The best peak signal-to-noise ratio (PSNR) at a fixed tube voltage of 70 kVp was obtained at tube currents of 20 and 32 mA, whereas the best PSNR at a fixed tube current of 25 mA was obtained at a tube voltage of 73 kVp. The fixed tube voltage of 70 kVp and the fixed tube current of 25 mA could help to obtain the best image quality and depict the spatial resolution of an anthropomorphic torso phantom radiographic examination. The normalized data over the DAP were provided to determine the patient dose from radiography.     

BiodosEPR-2006 Meeting: Acute dosimetry consensus committee recommendations on biodosimetry applications in events involving uses of radiation by terrorists and radiation accidents

In the aftermath of a radiological terrorism incident or mass-casualty radiation accident, first responders and receivers require prior guidance and pre-positioned resources for assessment, triage and medical management of affected individuals [NCRP, 2005. Key elements of preparing emergency responders for nuclear and radiological terrorism. NCRP Commentary No. 19, Bethesda, Maryland, USA]. Several recent articles [Dainiak, N., Waselenko, J.K., Armitage, J.O., MacVittie, T.J., Farese, A.M., 2003. The hematologist and radiation casualties. Hematology (Am. Soc. Hematol. Educ. Program) 473–496; Waselenko, J.K., MacVittie, T.J., Blakely, W.F., Pesik, N., Wiley, A.L., Dickerson, W.E., Tsu, H., Confer, D.L., Coleman, C.N., Seed, T., Lowry, P., Armitage, J.O., Dainiak, N., Strategic National Stockpile Radiation Working Group, 2004. Medical management of the acute radiation syndrome: recommendations of the Strategic National Stockpile Radiation Working Group. Ann. Intern. Med. 140(12), 1037–1051; Blakely, W.F., Salter, C.A., Prasanna, P.G., 2005. Early-response biological dosimetry—recommended countermeasure enhancements for mass-casualty radiological incidents and terrorism. Health Phys. 89(5), 494–504; Goans, R.E., Waselenko, J.K., 2005. Medical management of radiation casualties. Health Phys. 89(5), 505–512; Swartz, H.M., Iwasaki, A., Walczak, T., Demidenko, E., Salikhov, I., Lesniewski, P., Starewicz, P., Schauer, D., Romanyukha, A., 2005. Measurements of clinically significant doses of ionizing radiation using non-invasive in vivo EPR spectroscopy of teeth in situ. Appl. Radiat. Isot. 62, 293–299; Weisdorf, D., Chao, N., Waselenko, J.K., Dainiak, N., Armitage, J.O., McNiece, I., Confer, D., 2006. Acute radiation injury: contingency planning for triage, supportive care, and transplantation. Biol. Blood Marrow Transplant. 12(6), 672–682], national [National Council of Radiation Protection and Measurements (NCRP), 1994. Management of persons accidentally contaminated with radionuclides. NCRP Report No. 65, Bethesda, Maryland, USA; NCRP, 2001. Management of terrorist events involving radioactive material. NCRP Report No. 138, Bethesda, Maryland, USA; NCRP, 2005. Key elements of preparing emergency responders for nuclear and radiological terrorism. NCRP Commentary No. 19, Bethesda, Maryland, USA] and international [IAEA, 2005. Generic procedures for medical response during a nuclear or radiological emergency. EPR-Medical 2005, IAEA, Vienna, Austria] agencies have reviewed strategies for acute-phase biodosimetry. Consensus biodosimetric guidelines include: (a) clinical signs and symptoms, including peripheral blood counts, time to onset of nausea and vomiting and presence of impaired cognition and neurological deficits, (b) radioactivity assessment, (c) personal and area dosimetry, (d) cytogenetics, (e) in vivo electron paramagnetic resonance (EPR) and (f) other dosimetry approaches (i.e. blood protein assays, etc.). Emerging biodosimetric technologies may further refine triage and dose assessment strategies. However, guidance is needed regarding which biodosimetry techniques are most useful for different radiological scenarios and consensus protocols must be developed.The Local Organizing Committee for the Second International Conference on Biodosimetry and Seventh International Symposium on EPR Dosimetry and Applications (BiodosEPR-2006 Meeting) convened an Acute Dosimetry Consensus Committee composed of national and international experts to: (a) review the current literature for biodosimetry applications for acute-phase applications in radiological emergencies, (b) describe the strengths and weaknesses of each technique, (c) provide recommendations for the use of biodosimetry assays for selected defined radiation scenarios, and (d) develop protocols to apply these recommended biological dosimetry techniques with currently available supplies and equipment for first responders.The Acute Dosimetry Consensus Committee developed recommendations for use of a prioritized multiple-assay biodosimetric-based strategy, concluding that no single assay is sufficiently robust to address all of the potential radiation scenarios including management of mass casualties and diagnosis for early medical treatment. These recommendations may be used by first responders/first receivers that span time-windows of (i.e. 0–5 days) after the radiological incident for three radiological scenarios including: (a) radiation exposure device (RED), (b) radiological dispersal device (RDD), and (c) an improvised (or otherwise acquired) nuclear device (IND). Consensus protocols for various bioassays (i.e. signs and symptoms recording, bioassay sampling for radioactivity analysis, nail-clipping sampling for EPR analysis and blood collection for hematology, cytogenetics, and blood chemistry analyses) are presented as Appendix materials. As stated in NCRP Commentary No. 19 [NCRP, 2005. Key elements of preparing emergency responders for nuclear and radiological terrorism. NCRP Commentary No. 19, Bethesda, Maryland, USA], multi-parameter triage (i.e. time to vomiting, lymphocyte kinetics, and other biodosimetry indicators) offers the current best strategy for early assessment of absorbed dose.     

超流模型能级密度公式及其参数研究

对推广超流模型（GSM）能级密度公式作了研究,并根据评估的平均中子共振间距D0和分立能级累计数N0拟合出了一组GSM能级密度公式的参数,这组参数已被收入到国际原子能机构（IAEA）推荐输入参数库（RIPL）的起始数据文件. In this work, the Generalized Superfluid Model (GSM) level density formula has been studied. On the basis of the average neutron resonance level spacing D0 and cumulative level number N0 which were evaluated by ourselves, a set of GSM level density parameters has been obtained. These parameters have been included in the initial data file of IAEA s Reference Input Parameter Library (RIPL).     

Experimental investigation of commercially available lead composite aprons used for diagnostic X-rays

One of the principal diagnostic methods used in all fields of medical services is radiographic examination. To keep the radiation dose received by hospital personnel under normal working conditions as low as reasonably achievable, lead composite apron shields are provided as valuable aids. Intensive use of these accessories could lead to softening and surface defects due to poor handling and being worn-out over time, giving rise to multiple defects across the entire apron. Without routine control, these lead aprons will, within time, contribute significantly to the over-radiation burden to the wearer. However, local defects are highly likely to lead to gross changes in the radiation dose received by the wearer. For this reason, we evaluate the exposure dose resulting from diagnostic X-ray radiation during different imaging procedures. In this study, we used TLD LiF-700 chips to measure the attenuation percentage for four groups of commercial lead composite aprons and to calculate the effective doses to different organs during diagnostic radiological procedures. The results show the importance of lead composite aprons in minimizing effective doses, and the attenuation percentage varied for different vendors; this is due to variations in the constituent material. The average attenuation for lead composite aprons varies from 93.3% to 96.7%, and the average attenuation (%)/weight varies from 16.7% to 20.5%. Acceptance testing of lead composite aprons is essential to ensure that lead composite aprons meet their manufacturers’ specifications and provide the necessary radiation protection for their intended use. The combined and expanded uncertainties accompanying these measurements are 2.78% and 5.57%, respectively.     

Microfluidic Fabrication of Multi‐Drug‐Loaded Polymeric Microparticles for Topical Glaucoma Therapy

In this paper, the fabrication and characterization of multi‐drug‐loaded microparticles are demonstrated for topical glaucoma therapy. Specifically, latanoprost (“LAT”) and dexamethasone (“DEX”) are loaded in monodisperse microparticles (diameter ≈150 μm) of a biodegradable polymer–poly (lactic‐co‐glycolic) acid (PLGA)—using capillary microfluidics coupled with solvent evaporation. Both individual (LAT in PLGA and DEX in PLGA) and combined (LAT and DEX in PLGA) microparticle formulations are demonstrated. The morphology, size distribution and in vitro release kinetics are studied, and in vitro mucoadhesion of the formulated microparticles is also assessed. In addition, discussion is placed in how precise knowledge of the particle composition enabled by the microfluidic fabrication method and in vitro release rate measurements allow for facile topical formulation design and dose optimization. Such precision‐fabricated, multi‐drug loaded, sustained‐release microparticles are envisioned to serve as a promising platform for topical administration of ocular drugs. This could potentially reduce the frequency of eyedrop‐based drug administration from several times a day to merely once a day (or less), thus greatly facilitating patient compliance and adherence to a strict therapeutic drug regimen.     

Measurement of rectum dose by in vivo alanine/ESR dosimetry in gynecological 192Ir HDR brachytherapy

High dose rate (HDR) brachytherapy (BT) used in treatments of gynecological cancer often results in high doses in the pelvic organs at risk (OARs) and the complications in the rectum are a serious concern. Dosimetry procedures in vivo can be used as an evaluation method of calculated dose in treatment planning. One dosimetric method is the use of alanine with electron spin resonance (ESR) that has been used in different clinical practices. The aim of this study was to indicate the dose level in the female rectum volume, using alanine dosimeters during ¹⁹²Ir HDR gynecological BT, for cervical cancer. Doses were compared with the values obtained using the computational treatment planning system based on two orthogonal radiographic images. Firstly a phantom study in water was performed, enabling the in vivo study. Ten patients had the dose in rectum measured, resulting from 10 points properly referred; variations found were in the range of +60% and −50% of the delivered doses compared to the treatment planning system. Differences between planned and measured doses can be mainly due to uncertainty of dosimeter position determination, averaging of dose points specified over the whole dosimeter position, uncontrolled changes in detector position during treatment due to rectum movement and to a simplified treatment system planning, that do not take into account the details of the patient anatomy and the difference among the tissues. Results show that improvements of the protocol treatment should be done to enhance the relation between treatment planning system and experimental results, nevertheless the dose at the OARs was lower than the recommended by the ICRU Report 38.     

TLD occupational dose distribution study in nuclear medicine

The large and growing number of patients undergoing nuclear medicine procedures and workers involved in this practice requires continued efforts to improve the quality of diagnosis and to reduce the radiological risk associated. In Brazil, external individual monitoring with a dosemeter located on the thorax is compulsory for all workers in controlled areas. Extremity dosemeters are recommended in activities where hand dose can be much higher than on the thorax. This is typically the case for nuclear medicine procedures, but extremity dosemeters are not regularly used in Brazil. With the aim to study the occupational dose distribution in the nuclear medicine staff during their tasks of preparation and injection of radionuclides, Harshaw TLD chips of LiF:Mg,Cu,P (TLD-100H) were used for individual monitoring on different parts of the worker body. As expected, all doses measured on the thorax were much lower than on the hands. For both Single Photon Emission Computed Tomography (SPECT) and Positron Emission Tomography (PET), the dose to the fingers during preparation is about two orders of magnitude higher than in the thorax, and in the injection procedure, one order higher. The doses received by the workers' fingers varied widely depending on how they hold the syringe, but fingertips always received higher doses. The dose values measured in the eye lens were 200% higher than the one measured on the thorax.     

Conspicuity of bone metastases on fast Dixon-based multisequence whole-body MRI: Clinical utility per sequence

Purpose

The purpose of the study was to evaluate the conspicuity of bone metastases on each of the numerous sequences produced by fast Dixon-based multisequence whole-body (WB) magnetic resonance imaging (MRI) scanning in order to determine the most clinically useful sequences overall and per anatomic region.

Materials and Methods

Twenty-seven breast cancer patients with bone metastases were prospectively studied with fast Dixon-based WB MRI including head/neck, chest, abdominal, pelvic, thigh, calf/feet and either cervical, thoracic and lumbar or cervical/thoracic and thoracic/lumbar regions. Sequences included coronal T2, axial T1 without and with intravenous gadolinium (+ C), sagittal T1 spine + C, each associated fat-only (FO) and fat-saturated (FS) sequence, axial diffusion-weighted imaging (DWI) and short tau inversion recovery (STIR). Blinded reviewers evaluated lesion conspicuity, a surrogate of clinical utility, on a five-point scale per anatomic region. Sequences were compared using analysis of variance, differences were detected with Tukey's honestly significant difference test, and the four sequences with highest mean conspicuity were compared to the remainder overall and per anatomic region.

Results

Overall, a significant lesion conspicuity difference was found (P < .0001), and lesion conspicuity was significantly higher on FS T1 + C, FO T1 + C, T1 + C sagittal and FS T1 + C axial sequences (P < .0001). Per-region results were the same in the head/neck. Other sequences overlapped with these and included the following: chest/abdomen — FO T2, DWI; pelvis — DWI, FO T2; thigh — FS T2, FO T2, FO T1 + C; calf/feet — FS T2, DWI, FO T2, STIR.

Conclusion

Overall, bone lesions were most conspicuous on FS T1 + C sagittal, FO T1 + C sagittal, T1 + C sagittal and FS T1 + C axial fast Dixon WB MRI sequences.     

Study on the quality assurance of diagnostic X-ray machines and assessment of the absorbed dose to patients

Radiation exposure and image quality in X-ray diagnostic radiology provide a clear understanding of the relationship between the radiation dose delivered to a patient and image quality in optimizing medical diagnostic radiology. Because a certain amount of radiation is unavoidably delivered to patients, this should be as low as reasonably achievable. Several X-ray diagnostic machines were used at different medical diagnostic centers in Egypt for studying the beam quality and the dose delivered to the patient. This article studies the factors affecting the beam quality, such as the kilo-volt peak (kVp), exposure time (mSc), tube current (mAs) and the absorbed dose in (μGy) for different examinations. The maximum absorbed dose measured per mAs was 594±239 and 12.5±3.7 μGy for the abdomen and the chest, respectively, while the absorbed dose at the elbow was 18±6 μGy, which was the minimum dose recorded. The compound and expanded uncertainties accompanying these measurements were 4±0.35% and 8±0.7%, respectively. The measurements were done through quality control tests as acceptance procedures.     

Scatter radiation dose at the height of the operator's eye in interventional cardiology

This paper presents the first experimental result for scatter dose at the height of the operator's eye measured for a Polymethyl methacrylate (PMMA) phantom simulating an adult patient in an interventional laboratory at Belo Horizonte, Brazil. Values for scattered radiation doses at the height of the operator's eye are reported for procedures performed with and without a ceiling-suspended screen. Correlations between scatter radiation doses and different angiographic projections, phantom entrance dose and kerma area product, were obtained.Experimental measurements were made in an angiography X-ray system equipped with flat-panel detector. A cine and three fluoroscopy modes: low, medium and high dose were available. Scattered radiation doses were measured at three angiographic projections: anterior-posterior (AP), left anterior oblique 90° and left anterior oblique 45° with cranial 30° (spider) angulations. The detector measuring scatter radiation was positioned at the usual distance of the cardiologist's eye and the detector measuring phantom entrance dose was positioned at the bottom of the PMMA phantom.The phantom entrance dose for fluoroscopy low, medium, high and cine were 15, 29, 36 ± 4 and 184 ± 18 mGy/min, respectively to AP projection. A good linear correlation exists between phantom entrance doses rate and scatter dose rate to AP projection. There is a good linear correlation between the kerma-area product and scatter dose at the height of the operator's eye, coefficient of determination R² were 0.9728 and 0.9913 with and without ceiling-suspended screen. An experimental correlation factor of 0.1 and 3.5 μSv/Gy*cm2 has been found for the AP projection with and without ceiling-suspended screen, respectively. Scatter dose at the eyes cardiologist position depends on the C-arm angulation an increase of the scatter radiation dose by a factor of 5 was found.The highest dose rate in the lens was 19.74 ± 1.97 mSv/h without ceiling-suspended screen in cine mode for “spider” projection. For lateral projection in cine mode, the ceiling-suspended screen reduced dose by a factor 0.01. Interventional operator may therefore easily exceed the lens dose limit if ceiling-suspended screen is not used.     

On experiments to detect possible failures of relativity theory

Two recently proposed experiments by Kolen and Torr (K-T), designed to detect possible failures of Einstein's special relativity (SR) are analyzed. Imprecisions in these papers are pointed out. Computation in Lorentz aether theory (LAT), with the K-T violation of SR, of the theoretical prediction for the proposed K-T clock experiment prove their results to be incorrect. Analytical computation of the proposed K-T rotor Doppler experiment using LAT confirms the order of magnitude of their prediction by numerical computation. For LAT in the K-T form, this experiment, using a turntable, is shown to give results which could be detected with the technology of 20 years ago. Another LAT model is considered which leads to deviations of SR smaller than in the K-T model. The central point of the approach adopted in this paper is the use of Einstein's coordinates in the comoving frame, even when SR is violated, leading always to isotropic propagation of light in vacuum; the anisotropy usually obtained in LAT is only a coordinate effect.     

In vivo diffusion tensor imaging of thoracic and cervical rat spinal cord at 7 T

In vivo diffusion tensor imaging (DTI) of rat cervical and thoracic spinal cord was performed using a three-element phased array coil at 7 T. The magnetic field was shimmed over the spinal cord in real time using an in-house developed automatic algorithm. Echo planar imaging (EPI)-based diffusion-weighted images (DWIs) were acquired with 21 gradient encoding directions. The DWIs were tensor encoded, and diffusion tensor metrics, fractional anisotropy (FA), mean diffusivity (MD), longitudinal diffusivity (λ₀) and transverse diffusivity (λ) were determined for both white matter (WM) and gray matter (GM). The results on six normal rats indicated no significant differences in the diffusion tensor metrics between thoracic and cervical regions. However, the DTI-derived metrics in cervical spinal cord from our study are somewhat different from the published results in rats. The possible reasons for these differences are suggested.     

A review of: "Isotopes in Water Resources Management"

Abstract Proceedings of the International Symposium, organized by the IAEA, in co-operation with UNESCO, Vienna, 20-24 March 1995. Vol. 1 (ISBN 92-0-105595-1) and Vol. 2 (ISBN 92-0-100796-5), edited by IAEA, Vienna, Austria, March 1996.     

Activities in the IAEA X‐ray fluorescence laboratory at Seibersdorf

This paper describes the specific philosophy behind the functioning of the IAEA XRF Laboratory at Seibersdorf and its role in the XRF community. Some examples of the research and development activities and the results obtained are given. They include methodological development and construction of XRF instruments in order to extend the applicability range of the XRF technique, particularly in support of applications of the analytical technique in developing IAEA member states. The contribution of the laboratory to the training in methodology and applications of XRF techniques and to development of QA/QC procedures is also emphasised. Copyright © 2006 John Wiley & Sons, Ltd.     

A review of: “Isotopes in Water Resources Management”

Abstract

Proceedings of the International Symposium, organized by the IAEA, in co-operation with UNESCO, Vienna, 20–24 March 1995. Vol. 1 (ISBN 92-0-105595-1) and Vol. 2 (ISBN 92-0-100796-5), edited by IAEA, Vienna, Austria, March 1996.     

Effectiveness of Physical Therapy in Patients with Tension-type Headache: Literature Review

Background: Tension-type headache (TTH) is a disease with a great incidence on quality of life and with a significant socioeconomic impact. Objectives: The aim of this review is to determine the effectiveness of physical therapy by using manual therapy (MT) for the relief of TTH. Data sources: A review was done identifying randomized controlled trials through searches in MEDLINE, PEDro, Cochrane and CINAHL (January 2002 – April 2012). Study selection: English-language studies, with adult patients and number of subjects not under 11, diagnosed with episodic tension-type headache (ETTH) and chronic tension-type headache (CTTH) were included. Data extraction: Initial search was undertaken with the words Effectiveness, Tension-type headache, and Manual therapy (39 studies). In addition, a search which included terms related to treatments such as physiotherapy, physical therapy, spinal manipulation was performed (25 studies). Results: From the two searches 9 studies met the inclusion criteria and were analysed finding statistically significant results: 1) myofascial release, cervical traction, neck muscles trigger points in cervical thoracic muscles and stretching; 2) Superficial heat and massage, connective tissue manipulation and vertebral Cyriax mobilization; 3) cervical or thoracic spinal manipulation and cervical chin-occipital manual traction; 4) massage, progressive relaxation and gentle stretching, program of active exercises of shoulder, neck and pericranial muscles; 5) massage, passive rhythmic mobilization techniques, cervical, thoracic and lumbopelvic postural correction and cranio-cervical exercises; 6) progressive muscular relaxation combined with joint mobilization, functional, muscle energy, and strain/counterstrain techniques, and cranial osteopathic treatment; 7) massage focused on relieving myofascial trigger point activity; 8) pressure release and muscle energy in suboccipital muscles; 9) combination of mobilizations of the cervical and thoracic spine, exercises and postural correction. All studies used a combination of different techniques and none analyzed treatments separately, also all the studies have assessed aspects related to TTH beyond frequency and intensity of pain. Conclusions: The findings from these studies showed evidence that physiotherapy with articulatory MT, combined with cervical muscle stretching and massage are effective for this disease in different aspects related with TTH. No evidence was found of the effectiveness of the techniques applied separately.     

样本空间排序法评价瞬时辐照无失效数据研究

为降低瞬时辐照抽样检验中现行经典非参数法的评价结果的保守性,引入样本空间排序法,从理论和实例两方面计算无失效数据的生存概率置信下限,并与经典非参数法进行对比。结果是,样本空间排序法不仅提高生存概率的置信下限,还增大这些置信限所对应的剂量率范围。该研究结果表明样本空间排序法提高了数据利用率,降低了保守性,可减少试验成本。     

X‐ray fluorescence spectrometry in Dar es Salaam

Energy‐dispersive x‐ray fluorescence (EDXRF) analysis has been established at the University of Dar es Salaam, Faculty of Science, Department of Physics. Calibration was conducted using thin films from Micromatter (USA) for secondary target XRF. We report on the performance of the spectrometer including the detection limits attained, which range from 0.01 to 10 ng cm^?2 using collimators of 6 and 8 mm diameter under excitation conditions of 50 kV, 35 mA. The accuracy of the measurements was checked using IAEA SOIL‐7 and NIST 3087a Certified Reference Materials. The experimental values differed by <5% from the certified values. The total reflection x‐ray fluorescence (TXRF) facility added as a module to the existing XRF system provides detection limits between 0.1 and 100 pg for most of the elements measured. Copyright © 2005 John Wiley & Sons, Ltd.     

Evaluation of medical exposure and exposure by the public in a typical scenario of examinations using mobile X-ray equipment through the Monte Carlo simulation

In this work irradiation scenarios that simulated chest and abdomen examinations involving mobile X-ray equipment in hospitals were modeled with the purpose of calculating conversion coefficient for effective dose (CC_E), normalized to entrance surface dose (ESD), applied to patients and public individuals. These coefficients can easily be used in this practice. Patients and public individuals were represented by a pair of anthropomorphic phantoms inserted in the MCNPX 2.7.0 radiation transport code. One of the phantoms (patient) was irradiated with the direct beam simulating examinations of the chest and abdomen, each with two fields of irradiation, ideal (IF) and extrapolated (EF). Using the software SPECGEN X-ray spectra from 60 to 100 kVp at 10 kVp intervals were generated and used in this work. The other phantom (public individual) was positioned 50–200 cm from the patient. In relation to the CC_E calculated in the patient, the average increase obtained between the irradiation fields was 62.4% for the chest examinations, and for the same conditions the CC_E was calculated for abdomen examinations and found to be 8.0%. Increasing the distance between public individual and patient, reductions of up to 81.7% in the CC_E in abdomen examinations and 83.4% in chest examinations were observed. Through the assessment of CC_E of these scenarios, it is possible to measure the damages relating to this practice for both patients and public individuals.     

Quality assurance and quality control in light stable isotope laboratories: A case study of Rio Grande,Texas, water samples†

New isotope laboratories can achieve the goal of reporting the same isotopic composition within analytical uncertainty for the same material analysed decades apart by (1) writing their own acceptance testing procedures and putting them into their mass spectrometric or laser-based isotope-ratio equipment procurement contract, (2) requiring a manufacturer to demonstrate acceptable performance using all sample ports provided with the instrumentation, (3) for each medium to be analysed, prepare two local reference materials substantially different in isotopic composition to encompass the range in isotopic composition expected in the laboratory and calibrated them with isotopic reference materials available from the International Atomic Energy Agency (IAEA) or the US National Institute of Standards and Technology (NIST), (4) using the optimum storage containers (for water samples, sealing in glass ampoules that are sterilised after sealing is satisfactory), (5) interspersing among sample unknowns local laboratory isotopic reference materials daily (internationally distributed isotopic reference materials can be ordered at three-year intervals, and can be used for elemental analyser analyses and other analyses that consume less than 1 mg of material) – this process applies to H, C, N, O, and S isotope ratios, (6) calculating isotopic compositions of unknowns by normalising isotopic data to that of local reference materials, which have been calibrated to internationally distributed isotopic reference materials, (7) reporting results on scales normalised to internationally distributed isotopic reference materials (where they are available) and providing to sample submitters the isotopic compositions of internationally distributed isotopic reference materials of the same substance had they been analysed with unknowns, (8) providing an audit trail in the laboratory for analytical results – this trail commonly will be in electronic format and might include a laboratory information management system, (9) making at regular intervals a complete backup of laboratory analytical data (both of samples logged into the laboratory and of mass spectrometric analyses), being sure to store one copy of this backup offsite, and (10) participating in interlaboratory comparison exercises sponsored by the IAEA and other agencies at regular intervals.