Gerbil middle-ear sound transmission from 100 Hz to 60 kHz

Middle-ear sound transmission was evaluated as the middle-ear transfer admittance H(MY) (the ratio of stapes velocity to ear-canal sound pressure near the umbo) in gerbils during closed-field sound stimulation at frequencies from 0.1 to 60 kHz, a range that spans the gerbil's audiometric range. Similar measurements were performed in two laboratories. The H(MY) magnitude (a) increased with frequency below 1 kHz, (b) remained approximately constant with frequency from 5 to 35 kHz, and (c) decreased substantially from 35 to 50 kHz. The H(MY) phase increased linearly with frequency from 5 to 35 kHz, consistent with a 20-29 micros delay, and flattened at higher frequencies. Measurements from different directions showed that stapes motion is predominantly pistonlike except in a narrow frequency band around 10 kHz. Cochlear input impedance was estimated from H(MY) and previously-measured cochlear sound pressure. Results do not support the idea that the middle ear is a lossless matched transmission line. Results support the ideas that (1) middle-ear transmission is consistent with a mechanical transmission line or multiresonant network between 5 and 35 kHz and decreases at higher frequencies, (2) stapes motion is pistonlike over most of the gerbil auditory range, and (3) middle-ear transmission properties are a determinant of the audiogram.     

Swept-tone transient-evoked otoacoustic emissions

Transient-evoked otoacoustic emissions (TEOAE) are responses generated within the inner ear in response to acoustic stimuli and are indicative of normal cochlear function. They are commonly acquired by averaging post-stimulus acoustic responses recorded near the eardrum in response to brief stimuli such as clicks or tone pips. In this study a new long duration stimulus consisting of a frequency swept tone is introduced for the acquisition of TEOAEs. Like stimulus frequency generated OAEs, swept-tone responses contain embedded OAEs. With swept-tone analysis, OAEs can be recovered by convolving it with a time reversed swept-tone signal resulting in time-compression. In addition, higher order nonlinear OAE responses were removed from the linear TEOAE. The results show comparable phase and time-frequency properties between the click and swept-tone evoked OAEs. Swept-tone acquisition of TEOAEs has beneficial noise properties, improving the signal to noise ratio by 6 dB compared to click evoked responses thus offering testing time savings. Additionally, swept-tone analysis removed synchronized spontaneous OAE activity from the recordings of subjects exhibiting such responses in conventional click TEOAEs. Since swept-tone stimulus consists of a single frequency component at any instantaneous moment, its analysis also provides for direct comparison with stimulus-frequency OAEs and click evoked OAEs.     

Changes in auditory sensitivity with depth in a free-diving California sea lion (Zalophus californianus)

All current data on underwater hearing in pinnipeds are based on tests conducted in small tanks, and may not accurately represent the auditory functioning of free-ranging animals, especially if hearing sensitivity changes with water depth. Underwater auditory thresholds were determined for a California sea lion at depths ranging from 10 to 100 meters. The following results were obtained: (1) False alarm probabilities (responding in the absence of a signal) decreased significantly with depth, indicating that the sea lion adopted a more conservative response criterion in deeper water. (2) Hearing sensitivity generally worsened with depth. (3) There was a significant interaction between depth and frequency, the depth effect being most pronounced at 10 kHz and reversing at 35 kHz. Increasing pressure related to diving probably alters the impedance characteristics of the pinniped ear, in particular affecting the size of the middle-ear air space via expansion of cavernous tissue in the middle-ear cavity. These results show that the middle ear plays a functional role in underwater sound detection in sea lions. However, contrary to previous speculation, the presence of cavernous tissue in the sea lion middle ear does not appear to enhance sensitivity at depth.     

Further assessment of forward pressure level for in situ calibration

Quantifying ear-canal sound level in forward pressure has been suggested as a more accurate and practical alternative to sound pressure level (SPL) calibrations used in clinical settings. The mathematical isolation of forward (and reverse) pressure requires defining the The?venin-equivalent impedance and pressure of the sound source and characteristic impedance of the load; however, the extent to which inaccuracies in characterizing the source and/or load impact forward pressure level (FPL) calibrations has not been specifically evaluated. This study examined how commercially available probe tips and estimates of characteristic impedance impact the calculation of forward and reverse pressure in a number of test cavities with dimensions chosen to reflect human ear-canal dimensions. Results demonstrate that FPL calibration, which has already been shown to be more accurate than in situ SPL calibration, can be improved particularly around standing-wave null frequencies by refining estimates of characteristic impedance. Better estimates allow FPL to be accurately calculated at least through 10 kHz using a variety of probe tips in test cavities of different sizes, suggesting that FPL calibration can be performed in ear canals of all sizes. Additionally, FPL calibration appears a reasonable option when quantifying the levels of extended high-frequency (10-18 kHz) stimuli.     

Calibration of ear canals for audiometry at high frequencies

A procedure is described for determining the absolute sound pressure at the inner end of the ear canal when a sound source is coupled to the ear, for frequencies in the range 8-20 kHz. The transducer that generates the sound is coupled to the ear canal through a lossy tube, yielding a source impedance that is approximately matched to the characteristic impedance of the ear canal. A small microphone is located in the coupling tube close to the entrance to the ear canal. Calibration is carried out by measuring the response at this microphone when an impulse is applied at the transducer. To estimate the sound pressure at the medial end of the ear canal, the Fourier transform of this impulse response is corrected by an all-pole function in which the poles are estimated from the minima in this Fourier transform. Data on individual ear canals are presented in terms of gain functions relating the sound pressure at the medial end of the ear canal to the sound pressure when the coupling tube is blocked. The average gain function for a group of adult ears increases from 2 to 12 dB over the frequency range 8-20 kHz, in rough agreement with data from ear-canal models. Possible sources of error in the calibration procedure are discussed.     

Distortion-product otoacoustic-emission suppression tuning in human infants and adults using absorbed sound power

The greatest difference in distortion product otoacoustic emission (DPOAE) suppression tuning curves (STCs) in infant and adult ears occurs at a stimulus frequency of 6 kHz. These infant and adult STCs are much more similar when constructed using the absorbed power level of the stimulus and suppressor tones rather than using sound pressure level. This procedure incorporates age-related differences in forward and reverse transmission of sound power through the ear canal and middle ear. These results support the theory that the cochlear mechanics underlying DPOAE suppression are substantially mature in full-term infants.     

Theory of forward and reverse middle-ear transmission applied to otoacoustic emissions in infant and adult ears

The purpose of this study is to understand why otoacoustic emission (OAE) levels are higher in normal-hearing human infants relative to adults. In a previous study, distortion product (DP) OAE input/output (I/O) functions were shown to differ at f2 = 6 kHz in adults compared to infants through 6 months of age. These DPOAE I/0 functions were used to noninvasively assess immaturities in forward/reverse transmission through the ear canal and middle ear [Abdala, C., and Keefe, D. H., (2006). J. Acoust Soc. Am. 120, 3832-3842]. In the present study, ear-canal reflectance and DPOAEs measured in the same ears were analyzed using a scattering-matrix model of forward and reverse transmission in the ear canal, middle ear, and cochlea. Reflectance measurements were sensitive to frequency-dependent effects of ear-canal and middle-ear transmission that differed across OAE type and subject age. Results indicated that DPOAE levels were larger in infants mainly because the reverse middle-ear transmittance level varied with ear-canal area, which differed by more than a factor of 7 between term infants and adults. The forward middle-ear transmittance level was -16 dB less in infants, so that the conductive efficiency was poorer in infants than adults.     

Time-domain observation of otoacoustic emissions during constant tone stimulation.

Observation of the otoacoustic emissions (OAEs) evoked during a continuous single stimulus tone have been made on humans using a nonlinear residual time domain technique. The technique, described in this paper, involved the digital summation of responses to contiguous stimulation intervals, some of which included short bursts of a suppressor, or probe, tone. Stimulus intervals are constructed so that both the stimulus and probe tones summed to zero cyclically, leaving a residual response. This residual is attributable to the nonlinearity of the whole acoustic response, as measured in the ear canal, to the stimulus and probe tone complex. A theoretical treatment of this paradigm is presented examining the relation of this residual to the OAE evoked by the stimulus tone. It is shown experimentally that the residual, found at the stimulus tone frequency, has a latency and saturating input-output growth functions indicative of an OAE. The detailed OAE amplitude-versus-frequency variations, and the general latencies of the OAEs in two human ears were measured using both the constant tone evoked residual method described and the click evoked delayed emission method. The results from both methods are in agreement. The frequency-dependent properties of the suppression of the OAE were investigated using various stimuli to probe frequency ratios. The continuous tone time domain residual method has advantages for the observation of stimulus frequency OAEs and for relating these to any distortion product simultaneously generated.     

Ear asymmetries in middle-ear, cochlear, and brainstem responses in human infants

In 2004, Sininger and Cone-Wesson examined asymmetries in the signal-to-noise ratio (SNR) of otoacoustic emissions (OAE) in infants, reporting that distortion-product (DP)OAE SNR was larger in the left ear, whereas transient-evoked (TE)OAE SNR was larger in the right. They proposed that cochlear and brainstem asymmetries facilitate development of brain-hemispheric specialization for sound processing. Similarly, in 2006 Sininger and Cone-Wesson described ear asymmetries mainly favoring the right ear in infant auditory brainstem responses (ABRs). The present study analyzed 2640 infant responses to further explore these effects. Ear differences in OAE SNR, signal, and noise were evaluated separately and across frequencies (1.5, 2, 3, and 4 kHz), and ABR asymmetries were compared with cochlear asymmetries. Analyses of ear-canal reflectance and admittance showed that asymmetries in middle-ear functioning did not explain cochlear and brainstem asymmetries. Current results are consistent with earlier studies showing right-ear dominance for TEOAE and ABR. Noise levels were higher in the right ear for OAEs and ABRs, causing ear asymmetries in SNR to differ from those in signal level. No left-ear dominance for DPOAE signal was observed. These results do not support a theory that ear asymmetries in cochlear processing mimic hemispheric brain specialization for auditory processing.     

Frequency characteristics of sound transmission in middle ears from Norwegian cattle, and the effect of static pressure differences across the tympanic membrane and the footplate

For 23 cadaver ears from Norwegian cattle, frequency characteristics for the round-window volume displacement relative to the sound pressure at the eardrum have been measured, and are compared to earlier results for human ears [M. Kringlebotn and T. Gundersen, J. Acoust. Soc. Am. 77(1), 159-164 (1985)]. For human as well as for cattle ears, mean amplitude curves have peaks at about 0.7 kHz. At lower frequencies, the mean amplitude for cattle ears is about 5 dB smaller than for human ears. The amplitude curves cross at about 2 kHz, and toward higher frequencies the amplitude for cattle ears becomes increasingly larger. If amplitude curves are roughly approximated by straight lines above 1 kHz, the slope for cattle ears is about -5 dB/octave as compared to about -15 dB/octave for human ears. The phase of the round-window volume displacement lags behind the phase of the sound pressure at the tympanic membrane. The phase lag is close to zero below 0.2 kHz, but increases to about 3.5 pi at 20 kHz for cattle ears, as compared to less than 2 pi for human ears. Further investigations are needed in order to explain the observed differences. Sound transmission in the ear decreases with an increasing static pressure difference across the tympanic membrane, especially at frequencies below 1 kHz, where pressure differences of 10 and 60 cm water cause mean transmission losses of about 10 and 26 dB, respectively, the losses being somewhat larger for overpressures than for underpressures in the ear canal. At higher frequencies, the transmission losses are smaller. For small overpressures, and in a limited frequency range near 3 kHz, even some transmission enhancement may occur. Static pressure variations in the inner ear have only a minor influence on sound transmission. Static pressures relative to the middle ear in the range 0-60 cm water cause mean sound transmission losses less than 5 dB below 1 kHz, and negligible losses at higher frequencies.     

Finite-element analysis of middle-ear pressure effects on static and dynamic behavior of human ear

A finite-element analysis for static behavior of middle ear under variation of the middle-ear pressure was conducted in a 3D model of human ear by combining the hyperelastic Mooney-Rivlin material model and geometry nonlinearity. An empirical formula was then developed to calculate material parameters of the middle-ear soft tissues as the stress-dependent elastic modulus relative to the middle-ear pressure. Dynamic behavior of the middle ear in response to sound pressure in the ear canal was predicted under various positive and negative middle-ear pressures. The results from static analysis indicate that a positive middle ear pressure produces the static displacements of the tympanic membrane (TM) and footplate more than a negative pressure. The dynamic analysis shows that the reductions of the TM and footplate vibration magnitudes under positive middle-ear pressure are mainly determined by stress dependence of elastic modulus. The reduction of the TM and footplate vibrations under negative pressure was caused by both the geometry changes of middle-ear structures and the stress dependence of elastic modulus.     

Acoustic mechanisms that determine the ear-canal sound pressures generated by earphones

In clinical measurements of hearing sensitivity, a given earphone is assumed to produce essentially the same sound-pressure level in all ears. However, recent measurements [Voss et al., Ear and Hearing (in press)] show that with some middle-ear pathologies, ear-canal sound pressures can deviate by as much as 35 dB from the normal-ear value; the deviations depend on the earphone, the middle-ear pathology, and frequency. These pressure variations cause errors in the results of hearing tests. Models developed here identify acoustic mechanisms that cause pressure variations in certain pathological conditions. The models combine measurement-based Thévenin equivalents for insert and supra-aural earphones with lumped-element models for both the normal ear and ears with pathologies that alter the ear's impedance (mastoid bowl, tympanostomy tube, tympanic-membrane perforation, and a "high-impedance" ear). Comparison of the earphones' Thévenin impedances to the ear's input impedance with these middle-ear conditions shows that neither class of earphone acts as an ideal pressure source; with some middle-ear pathologies, the ear's input impedance deviates substantially from normal and thereby causes abnormal ear-canal pressure levels. In general, for the three conditions that make the ear's impedance magnitude lower than normal, the model predicts a reduced ear-canal pressure (as much as 35 dB), with a greater pressure reduction with an insert earphone than with a supra-aural earphone. In contrast, the model predicts that ear-canal pressure levels increase only a few dB when the ear has an increased impedance magnitude; the compliance of the air-space between the tympanic membrane and the earphone determines an upper limit on the effect of the middle-ear's impedance increase. Acoustic leaks at the earphone-to-ear connection can also cause uncontrolled pressure variations during hearing tests. From measurements at the supra-aural earphone-to-ear connection, we conclude that it is unusual for the connection between the earphone cushion and the pinna to seal effectively for frequencies below 250 Hz. The models developed here explain the measured pressure variations with several pathologic ears. Understanding these mechanisms should inform the design of more accurate audiometric systems which might include a microphone that monitors the ear-canal pressure and corrects deviations from normal.     

Specification of absorbed-sound power in the ear canal: application to suppression of stimulus frequency otoacoustic emissions

An insert ear-canal probe including sound source and microphone can deliver a calibrated sound power level to the ear. The aural power absorbed is proportional to the product of mean-squared forward pressure, ear-canal area, and absorbance, in which the sound field is represented using forward (reverse) waves traveling toward (away from) the eardrum. Forward pressure is composed of incident pressure and its multiple internal reflections between eardrum and probe. Based on a database of measurements in normal-hearing adults from 0.22 to 8 kHz, the transfer-function level of forward relative to incident pressure is boosted below 0.7 kHz and within 4 dB above. The level of forward relative to total pressure is maximal close to 4 kHz with wide variability across ears. A spectrally flat incident-pressure level across frequency produces a nearly flat absorbed power level, in contrast to 19 dB changes in pressure level. Calibrating an ear-canal sound source based on absorbed power may be useful in audiological and research applications. Specifying the tip-to-tail level difference of the suppression tuning curve of stimulus frequency otoacoustic emissions in terms of absorbed power reveals increased cochlear gain at 8 kHz relative to the level difference measured using total pressure.     

High-frequency audiometric assessment of a young adult population

The hearing thresholds of 37 young adults (18-26 years) were measured at 13 frequencies (8, 9,10,...,20 kHz) using a newly developed high-frequency audiometer. All subjects were screened at 15 dB HL at the low audiometric frequencies, had tympanometry within normal limits, and had no history of significant hearing problems. The audiometer delivers sound from a driver unit to the ear canal through a lossy tube and earpiece providing a source impedance essentially equal to the characteristic impedance of the tube. A small microphone located within the earpiece is used to measure the response of the ear canal when an impulse is applied at the driver unit. From this response, a gain function is calculated relating the equivalent sound-pressure level of the source to the SPL at the medial end of the ear canal. For the subjects tested, this gain function showed a gradual increase from 2 to 12 dB over the frequency range. The standard deviation of the gain function was about 2.5 dB across subjects in the lower frequency region (8-14 kHz) and about 4 dB at the higher frequencies. Cross modes and poor fit of the earpiece to the ear canal prevented accurate calibration for some subjects at the highest frequencies. The average SPL at threshold was 23 dB at 8 kHz, 30 dB at 12 kHz, and 87 dB at 18 kHz. Despite the homogeneous nature of the sample, the younger subjects in the sample had reliably better thresholds than the older subjects. Repeated measurements of threshold over an interval as long as 1 month showed a standard deviation of 2.5 dB at the lower frequencies (8-14 kHz) and 4.5 dB at the higher frequencies.     

Middle ear cavity and ear canal pressure-driven stapes velocity responses in human cadaveric temporal bones

Drive pressure to stapes velocity (V(st)) transfer function measurements are collected and compared for human cadaveric temporal bones with the drive pressure alternately on the ear canal (EC) and middle ear cavity (MEC) sides of the tympanic membrane (TM), in order to predict the performance of proposed middle-ear implantable acoustic hearing aids, as well as provide additional data for examining human middle ear mechanics. The chief finding is that, in terms of the V(st) response, MEC stimulation performs at least as well as EC stimulation below 8 kHz, provided that the EC is unplugged. Plugging the EC causes a reduced response for MEC drive below 2 kHz, due to a corresponding reduction of the pressure difference between the two sides of the TM. Between 8 and 11 kHz, the MEC drive transfer functions feature an approximately 17 dB drop in magnitude below the EC drive case, the cause of which remains unknown. The EC drive transfer functions reported here feature significantly less magnitude roll-off above 1 kHz than previous studies [with a slope of -2.3 vs -6.7 dB/octave for Aibara et al., Hear. Res. 152, 100-109 (2001)], and significantly more phase group delay (134 vs 62 micros for Aibara et al.).     

Wideband reflectance tympanometry in normal adults.

Acoustic impedance/reflectance measurements were made at various ear-canal pressures in 20 subjects with a clinical acoustic immittance instrument and an experimental impedance/reflectance system. Measurements were made over a frequency range of 226-2000 Hz with the clinical system and 125-11,310 Hz with the experimental system. For frequencies < or = 2.0 kHz, tympanograms obtained with the two systems are similar, with patterns that progress through the same orderly sequence with increasing frequency. Eardrum impedance measurements were also similar. There are small gender differences in middle-ear impedance. Reflectance patterns (reflectance versus frequency) at ambient ear-canal air pressure are characterized by high reflectance at low frequencies, two district minima at 1.2 and 3.5 kHz, increasing reflectance to 8.0 kHz, and decreasing reflectance above that frequency. Ear-canal pressure increases reflectance at low frequencies, decreases reflectance in the region of the minimum, and increases reflectance slightly at high frequencies. Reflectance tympanograms (reflectance versus ear-canal pressure) progress through a sequence of three patterns. At low frequencies, reflectance tympanograms are "V" shaped, indicating that pressure increases reflectance. At frequencies near the minimum reflectance, the pattern inverts, indicating that pressure decreases reflectance. At high frequencies, the patterns are flat, indicating that ear-canal pressure has little effect. Results presented for one patient suggest that reflectance tympanometry may be useful for detecting middle-ear pathology.     

The spatial distribution of sound pressure within scaled replicas of the human ear canal

A theoretical model for calculating the variation of sound pressure within the ear canal is presented. The theory is an extension of the horn equation approach, and accounts for the variation of cross-sectional area and curvature of the ear canal along its length. Absorption of acoustic energy at the eardrum is included empirically through an effective eardrum impedance that acts at a single location in the canal. For comparison, measurements of the distribution of sound pressure have been made in two replica ear canals. Both replicas have geometries that duplicate, as nearly as possible, that of a real human ear canal, except that they have been scaled up in size to increase the precision of measurements. One of the replicas explicitly contains a load impedance to provide acoustical absorption at a single eardrum position. Agreement between theory and experiment was good. It is clear that at higher frequencies (above about 6 kHz in human ear canals), this theoretical approach is preferable to the more usual "uniform cylinder" approximation for the ear canal. At higher frequencies, there is no unique eardrum pressure; rather, very large variations of sound pressure are found over the tympanic membrane surface.     

Effects of draining cochlear fluids on stapes displacement in human middle-ear models.

Displacement-frequency characteristics of the stapes footplate were measured in five human temporal bones before and after draining the vestibule. Measurements were made in the 0.125-8 kHz range at 80 dB input sound pressure level, using a laser Doppler vibrometer. A circuit model was also used to predict stapes displacement. The temporal bone studies show a slight decrease in stapes footplate displacement at low frequency, and little change above 1 kHz. The displacement change is not as great as that found by other investigators or predicted by the model. There is little difference in stapes motion in temporal bones when the inner ear is intact or drained.     

Resonant modes in transiently evoked otoacoustic emissions and asymmetries between left and right ear

A number of single-frequency resonant modes in click evoked otoacoustic emissions (OAEs) was investigated. The OAE modes were identified by means of an adaptive approximation method based on the matching pursuit (MP) algorithm. The signals were decomposed into basic waveforms coming from a very large and redundant dictionary of Gabor functions. The study was performed on transiently evoked otoacoustic emissions (TEOAEs) from left and right ears of 108 subjects. The correspondence between waveforms found by the procedure and resonant modes was shown (both for simulated noisy data and for single-person TEOAEs). The decomposition of TEOAEs made distinction between short and long-lasting components possible. The number of main resonant modes was studied by means of different criteria and they all led to similar results, indicating that the main features of the signal are explained on average by 10 waveforms. The same number of resonant modes for the right ear accounted for more energy than for the left ear.