不同产地中药赤芍在振荡体系中的化学指纹图谱研究

采用H2 SO4 - CH2 (COOH)2 - Ce2( SO4)3- KBrO3化学振荡体系,研究了中药赤芍的化学指纹图谱,并对温度、赤芍用量进行了考察,确定体系的最佳实验条件为12 mL 3.0 mol/L H2SO4溶液、6 mL 0.4 mol/LCH2( COOH)2溶液、3 mL 0.005 mol/L ...     

大黄的非线性化学指纹图谱研究

通过别洛索夫-扎鲍京斯基(B-Z)振荡反应对不同来源大黄进行定性分析,将大黄粉末加入H2SO4-MnSO4-CH3COCH3-NaBrO3中,组成化学振荡体系,用电化学工作站记录数据,并对化学试剂的浓度、反应温度、大黄用量等条件进行了考察,确定体系的最佳实验条件,获得了不同来源大黄的非线性化学指纹图谱.通过对大黄非线性化学指纹图谱的研究,发现不同来源的大黄的指纹图谱主要参数有较大区别.该方法简便,准确,可靠,可方便地用于不同来源大黄的定性分析.     

复合有机底物化学振荡体系的中药非线性电化学指纹图谱研究

本文提出了BrO3-Ce4+-H2SO4-丙二酸/苹果酸复合有机底物化学振荡体系,并将其用于研究中药的电化学指纹图谱.考察了振荡体系中各组分的浓度、温度、中药加入量、浸泡时间等因素对振荡图谱特异性的影响,确定了最佳组成及测试条件.研究结果表明,非线性电化学指纹图谱可用于鉴别和评价中药的真伪、药效、道地性等.该方法快速、...     

阿莫西林对B-Z振荡反应的影响及其振荡指纹图谱

研究了阿莫西林对B-Z振荡反应的影响,结果表明,阿莫西林的加入明显地改变了振荡体系的周期和振幅,即对振荡体系产生扰动,且阿莫西林的浓度分别在1.5×10-5~2.4×10-3mol/L和4.7×10-5~4.8×10-3mol/L范围内与振荡周期改变值Δt和振幅改变值ΔA均呈良好的线性关系,相关系数分别为0.9822和0.9846,同时结合FKN机理,初步探讨了阿莫西林扰动B-Z振荡反应的机理。     

高效液相色谱指纹图谱应用于板蓝根的鉴定

利用高效液相色谱建立了板蓝根的色谱指纹图谱。采用反相C18柱，流动相为水：甲醇（V/V=96：4），检测波长为260nm；流速1.0mL/min进行试验。根据相对保留值a和相对面积Sr对色谱指纹图谱进行分析对比研究，建立了一个快速、简易鉴别板蓝根样品的色谱指纹图谱分析方法。该法为中药样品的鉴定提供了较全面的信息，并为HPLC在复杂组分的样品分析和鉴别领域开拓了新的应用。     

中草药板蓝根粉末的X射线衍射傅立叶指纹图谱分析

对来自不同地区的板蓝根粉末样品用X射线衍射傅立叶(Fourier)指纹图谱技术进行鉴定.由于不同产地的板蓝根样品的指纹图谱不同,因此,采用这一技术可对板蓝根样品作出区别和鉴定.试验结果表明该方法尚可应用于其他中药的鉴别.     

中药指纹图谱技术

介绍中药指纹图谱的研究内容、意义和特点 ,以及中药指纹图谱采集和信息处理的主要技术手段     

复方板蓝根颗粒抗病毒成分HPLC-UV指纹图谱及LC-ESI-MSn研究

建立了复方板蓝根颗粒提取物高效液相色谱(HPLC-UV)指纹图谱分析方法. 确定了10批不同来源复方板蓝根颗粒提取物的19个共有峰. 各提取物的HPLC-UV指纹图谱与对照指纹图谱比较, 相似度均在93%以上. 利用液相色谱与质谱联用(LC-ESI-MSⁿ)技术对主要共有峰的结构进行了鉴定. 药效学研究表明, 复方板蓝根颗粒提取物有抗病毒活性. 该指纹图谱精密度、稳定性和重现性良好, 可作为复方板蓝根颗粒的质量评价方法.     

中药指纹图谱技术研究现状

中药指纹图谱特指中药样本经光谱或色谱等现代分析技术得到的中药各组分群体特征的图谱或图象。凭借实用的指纹图谱既可确认中药材或中药制剂的真伪,同时又能判断其质量的稳定与否。本文对中药指纹图谱技术研究领域内的国内外现状及主要技术进行了综述。     

薄层扫描法测定板蓝根中精氨酸含量

板蓝根中除含有靛蓝、靛玉红、β－谷甾醇、锌、铜、铁、镁、钾等25种物质之外,还含有14种氨基酸,总氨基酸含量2．75％,其中精氨酸含量最高（1．94％）^[1-7],本文采用薄层扫描法分析了精氨酸含量。     

Improved liquid chromatography fingerprint of fat-soluble Radix isatidis extract using multi-wavelength combination technique

We first investigated liquid chromatography (LC) fingerprint method using multi-wavelength combination technique, and successfully used this method for the analysis of a fat-soluble extract from Radix isatidis. LC fingerprints of fat-soluble R. isatidis extracts from 11 origins were established using the Origin software and Similarity Evaluation System for Chromatographic Fingerprints of traditional Chinese medicine (TCM). The typical LC fingerprints of fat-soluble extracts from R. isatidis were first established, and the reference chromatogram was also generated with 24 common peaks showing large peak areas and good separation from adjacent peaks. Seven common characteristic peaks were identified for the first time: anthranilic acid, syringic acid, benzoic acid, salicylic acid, tryptanthrin, indigo and indirubin. The total peak areas of 24 common peaks were more than 80% of the total peak areas. Hierarchical clustering analysis (HCA) of 11 R. isatidis samples was performed, and the results show that the differences between 11 origin R. isatidis were large. Principal component analysis (PCA) on 24 common peaks was obtained to find the possible chemical markers for the discrimination of different samples. The loading plot indicated that peaks 8, 11, 13 and 14 may have more influence on the discrimination of the samples. All these were useful for evaluating and controlling the quality of R. isatidis. Our work provides a general model of chromatogram combination at multi-wavelength detection to study the complex or the undeveloped materials, which can be used to scientifically ensure the quality of such samples and deeply do qualitative, quantitative and multicomponent pharmacodynamic research combined with modern advanced chromatographic technique.     

Comparison of reversed‐phase liquid chromatography and hydrophilic interaction chromatography for the fingerprint analysis of Radix isatidis

Radix isatidis is a famous anti‐influenza virus herbal medicine traditionally taken as a water decoction. However, the chemical fingerprint analysis of Radix isatidis is dominantly based on RPLC, from which it is difficult to obtain fingerprint information of hydrophilic compounds. Here, we developed the separation of Radix isatidis by RPLC and hydrophilic interaction chromatography, comparing the traditional RPLC fingerprint with the hydrophilic interaction chromatography fingerprint. Besides, an anti‐viral assay of Radix isatidis was conducted to evaluate its efficacy. The fingerprint–efficacy relationships between the fingerprints and the anti‐viral activity were further investigated with principal component regression analysis. The results showed that the anti‐viral activity correlated better with the hydrophilic interaction chromatography fingerprint than with the RPLC fingerprint. This study indicates that hydrophilic interaction chromatography could not only be a complementary method to increase the fingerprint coverage of conventional RPLC fingerprint, but also can better represent the efficacy and quality of Radix isatidis.     

甘草参与的B-Z振荡反应研究

以H+-Mn2+-BrO-3-CH3COCH3-甘草组成的化学振荡体系,应用电化学工作站记录电位(E)随时间(t)的变化,绘制化学振荡曲线.通过考察反应条件及反应物的浓度等因素对振荡曲线的影响,优化最佳的振荡反应条件,计算反应的活化能,推断反应的机理.为利用此方法鉴别中草药及研究中药治疗疾病提供理论依据.     

Study on the oscillating phenomena of electrical potential across a liquid membrane

The electrical oscillations across a liquid membrane in water/oil/water system was studied with octanol as oil phase by introducing two opposite charged surfactants in oil and aqueous phase, respectively. The sustained and rhythmic oscillation was observed. To a certain extent, the features of the oscillation (e.g. induction time, frequency, life time and orientation of the pulse pikes) strongly depend on the property of surfactant, dissolved in octanol. The mechanism may be explained by the formation and destruction of dual-ion surfactant membrane accompanying with emulsification at the interface and considering the coupling effect of diffusion and associated reaction in the vicinity of the interface.     

基于多元统计学的青稞HPLC指纹图谱研究

为了筛选品质良好的青稞,选择不同产地的10批青稞样品,建立了青稞的高效液相指纹图谱,并进行了聚类分析和主成分分析,测定了儿茶素、表儿茶素和芦丁3种指标成分的含量.首先采用高效液相色谱法测得了10批青稞的色谱图,建立其共有模式,确定了11个共有峰,蓝青稞指标成分含量均高于白青稞.对共有峰数据进行聚类分析和主成分分析,10...     

唐古特大黄乙醇提取物的HPLC指纹图谱研究

用高效液相色谱法建立唐古特大黄乙醇提取物的指纹图谱分析方法。采用Phenomenex Kromasil C18色谱柱(4.6 mm×250 mm,5μm);甲醇-0.1%H3PO4为流动相,梯度洗脱,检测波长为270 nm,以大黄酸为参照峰。结果共有18个共有峰。此法为有效地控制唐古特大黄乙醇提取物的质量提供了依据。